Microbial community structure of activated sludge during aerobic granulation in an annular gap bioreactor

2006 ◽  
Vol 54 (1) ◽  
pp. 139-146 ◽  
Author(s):  
J.C. Williams ◽  
F.L. de los Reyes
Keyword(s):  
Loading Rate ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Granular Sludge ◽  
Aerobic Granular Sludge ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Annular Gap ◽  
Gradient Gel Electrophoresis ◽  
And Behavior ◽  
Cultivation Experiment

A novel annular gap reactor was designed to create a controlled shear environment in which aerobic granular sludge could be developed. The bacterial and eukaryal community structures during two aerobic granular sludge experiments were tracked using denaturing gradient gel electrophoresis (DGGE). The first granule cultivation experiment, using an organic loading rate of 1.6 kg/m3d COD, resulted in biomass that was dominated by filamentous bacteria and Zoogloea ramigera colonies. A second experiment with a higher organic loading rate of 6 kg/m3d COD developed a granule-like morphology but was ultimately dominated by filamentous fungi. Species identification via DGGE band purification and DNA sequencing closely matched the observed sludge morphology and behavior.

Aerobic granular sludge in an SBR-system treating wastewater rich in particulate matter

2004 ◽  
Vol 49 (11-12) ◽  
pp. 41-46 ◽  
Author(s):  
N. Schwarzenbeck ◽  
R. Erley ◽  
P.A. Wilderer
Keyword(s):  
Particle Size ◽  
Organic Matter ◽  
Primary Particle ◽  
Loading Rate ◽  
Granular Sludge ◽  
Aerobic Granular Sludge ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Particle Uptake ◽  
Tracer Experiments

Aerobic granular sludge was successfully cultivated in a lab-scale SBR-system treating malting wastewater with a high content of particulate organic matter (0.9 gTSS/L). At an organic loading rate (CODtotal) of 3.4 kg/(m3·d) an average removal efficiency of 50% in CODtotal and 80% in CODdissolved was achieved. Fractionation of the COD by means of particle size showed that particles with a diameter less than 25–50 μm could be removed at 80% efficiency, whereas particles bigger than 50 μm were only removed at 40% efficiency. Tracer experiments revealed a dense sessile protozoa population covering the granules. The protozoa appeared to be responsible for primary particle uptake from the wastewater.

Aerobic granular sludge: a promising technology for decentralised wastewater treatment

2006 ◽  
Vol 53 (9) ◽  
pp. 79-85 ◽  
Author(s):  
Z.H. Li ◽  
T. Kuba ◽  
T. Kusuda
Keyword(s):  
Sequencing Batch Reactor ◽  
Loading Rate ◽  
Batch Reactor ◽  
Granular Sludge ◽  
Volume Index ◽  
Synthetic Wastewater ◽  
Aerobic Granular Sludge ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Fast Settling

In order to evaluate the characteristics of aerobic granular sludge, a sequencing batch reactor, feeding with synthetic wastewater at the organic loading rate of 8 kg COD/m3 d, was employed on the laboratory scale. Granules occurred in the reactor within 1 week after the inoculation from conventional flocculent sludge. Aerobic granular sludge was characterised by the outstanding settling properties and considerable contaminates removal efficiencies. The SVI30 values were in the range of 20 to 40 ml g−1. However, the sludge volume index of short settling time (e.g. SVI10 – 10 min) is suggested to describe the fast settling properties of aerobic granular sludge. The potential application in the decentralised system is evaluated from the point view of footprint and high bioactivity. The occurrence of sloughing, resulting from the outgrowth of filamentous organisms, would be responsible for the instability of aerobic granules. The starvation phase should therefore be carefully controlled for the maintenance and stability of aerobic granular sludge system.

scholarly journals Characterization of Aerobic Granular Sludge Developed under Variable and Low Organic Loading Rate

2017 ◽  
Vol 46 (12) ◽  
pp. 2497-2506 ◽  
Author(s):  
Nik Azimatolakma Awang ◽  
Md Ghazaly Shaaban ◽  
Choon Weng Lee ◽  
Bong Chui Wei
Keyword(s):  
Loading Rate ◽  
Granular Sludge ◽  
Aerobic Granular Sludge ◽  
Organic Loading Rate ◽  
Organic Loading

Performance and stability of an expanded granular sludge bed reactor modified with zeolite addition subjected to step increases of organic loading rate (OLR) and to organic shock load (OSL)

2017 ◽  
Vol 77 (1) ◽  
pp. 39-50 ◽  
Author(s):  
T. Pérez-Pérez ◽  
I. Pereda-Reyes ◽  
E. Pozzi ◽  
D. Oliva-Merencio ◽  
M. Zaiat
Keyword(s):  
Volatile Fatty Acids ◽  
Loading Rate ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Stable Process ◽  
Oxygen Demand ◽  
Granular Sludge ◽  
Swine Wastewater ◽  
Organic Loading Rate ◽  
Organic Loading ◽  
Microbial Composition

Abstract This paper shows the effect of organic shock loads (OSLs) on the anaerobic digestion (AD) of synthetic swine wastewater using an expanded granular sludge bed (EGSB) reactor modified with zeolite. Two reactors (R1 and R2), each with an effective volume of 3.04 L, were operated for 180 days at a controlled temperature of 30 °C and hydraulic retention time of 12 h. In the case of R2, 120 g of zeolite was added. The reactors were operated with an up-flow velocity of 6 m/h. The evolution of pH, total Kjeldahl nitrogen, chemical oxygen demand (COD) and volatile fatty acids (VFAs) was monitored during the AD process with OSL and increases in the organic loading rate (OLR). In addition, the microbial composition and changes in the structure of the bacterial and archaeal communities were assessed. The principal results demonstrate that the presence of zeolite in an EGSB reactor provides a more stable process at higher OLRs and after applying OSL, based on both COD and VFA accumulation, which presented with significant differences compared to the control. Denaturing gradient gel electrophoresis band profiles indicated differences in the populations of Bacteria and Archaea between the R1 and R2 reactors, attributed to the presence of zeolite.

Tolerance to organic loading rate by aerobic granular sludge in a cyclic aerobic granular reactor

2015 ◽  
Vol 182 ◽  
pp. 314-322 ◽  
Author(s):  
Bei Long ◽  
Chang-zhu Yang ◽  
Wen-hong Pu ◽  
Jia-kuan Yang ◽  
Fu-biao Liu ◽  
...  
Keyword(s):  
Loading Rate ◽  
Granular Sludge ◽  
Aerobic Granular Sludge ◽  
Organic Loading Rate ◽  
Organic Loading

Performance of an anaerobic baffled reactor (ABR) as a hydrolysis-acidogenesis unit in treating landfill leachate mixed with municipal sewage

2000 ◽  
Vol 42 (12) ◽  
pp. 115-121 ◽  
Author(s):  
B. Wang ◽  
Y. Shen
Keyword(s):  
Landfill Leachate ◽  
Loading Rate ◽  
Granular Sludge ◽  
Operational Performance ◽  
Municipal Sewage ◽  
Organic Loading Rate ◽  
Stable Operation ◽  
Organic Loading ◽  
Anaerobic Baffled Reactor ◽  
Biological Treatability

A study on the performance of an Anaerobic Baffled Reactor(ABR) as a hydrolysis-acidogenesis unit in treating the mixed wastewater of landfill leachate and municipal sewage in different volumetric ratios was carried out. The results showed that ABR substantially improved the biological treatability of the mixed wastewater by increasing its BOD5/COD ratio to 0.4–0.6 from the initial values of 0.15–0.3. The formation of bar-shaped granular sludge of 0.5–5 mm both in diameter and length with an SVI of 7.5–14.2 ml/g was observed in all compartments of the ABR when the organic loading rate reached 4.71 kgCOD/m3 · d. The effects of the ratios of NH4+-N/COD and COD/TP in mixed wastewater on the operational performance were also studied, from which it was found that a reasonable NH4+-N/COD ratio should be lower than 0.02, and the phosphorus supplement was needed when the volumetric ratio was higher than 4:6 for stable operation of ABR.

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