Effect of side-stream phosphorus recovery on biological phosphorus removal performance investigated by chemical and microbial analyses in a novel BNR-IC process

2014 ◽  
Vol 70 (9) ◽  
pp. 1441-1447 ◽  
Author(s):  
H. M. Zou ◽  
X. W. Lu ◽  
T. Li
Keyword(s):  
Microbial Community ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Phosphorus Recovery ◽  
Biological Phosphorus Removal ◽  
Side Stream ◽  
Negative Effect ◽  
Gradient Gel Electrophoresis ◽  
P Recovery ◽  
Biological Phosphorus ◽  
P Removal

The aim of this study was to assess the effect of side-stream ratio (SSR) on performance of phosphorus (P) removal and recovery in a novel process linking biological nutrients removal (BNR) and induced crystallization (IC). Results showed that P removal efficiency was significantly enhanced when given an appropriate SSR, resulting in effluent P concentrations decreasing from 0.75 to 0.39 mg/L with an increase of SSR from 0 to 35%, where a maximum of 7.19 mg/L P recovery amount was obtained at 35% of SSR. Increasing the SSR can favor the P recovery, while an excessively high SSR (more than 35%) would have a negative effect on the subsequent biological P removal in the BNR-IC system. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis showed that in total, 11 DGGE bands of highest species richness were visually detected and significant changes in microbial community structure were found, with SSR variations ranging from 0 to 55%. Moreover, an increase in SSR can cause an increase in microbial community biodiversity; where microbial populations correspond to the 11 bands, they were generally classified into five different phyla or classes (Beta-, Gamma-, and Deltaproteobacteria, as well as Clostridia and Flavobacteria) based on the evolutionary tree analysis.

Identification of a Novel Group of Bacteria in Sludge from a Deteriorated Biological Phosphorus Removal Reactor

1999 ◽  
Vol 65 (3) ◽  
pp. 1251-1258 ◽  
Author(s):  
Alex T. Nielsen ◽  
Wen-Tso Liu ◽  
Carlos Filipe ◽  
Leslie Grady ◽  
Søren Molin ◽  
...  
Keyword(s):  
Phosphorus Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Weight Ratio ◽  
Microscopic Analysis ◽  
The Novel ◽  
Biological Phosphorus Removal ◽  
Close Relationship ◽  
Gradient Gel Electrophoresis ◽  
Biological Phosphorus

ABSTRACT The microbial diversity of a deteriorated biological phosphorus removal reactor was investigated by methods not requiring direct cultivation. The reactor was fed with media containing acetate and high levels of phosphate (P/C weight ratio, 8:100) but failed to completely remove phosphate in the effluent and showed very limited biological phosphorus removal activity. Denaturing gradient gel electrophoresis (DGGE) of PCR-amplified 16S ribosomal DNA was used to investigate the bacterial diversity. Up to 11 DGGE bands representing at least 11 different sequence types were observed; DNA from the 6 most dominant of these bands was further isolated and sequenced. Comparative phylogenetic analysis of the partial 16S rRNA sequences suggested that one sequence type was affiliated with the alpha subclass of theProteobacteria, one was associated with theLegionella group of the gamma subclass of theProteobacteria, and the remaining four formed a novel group of the gamma subclass of the Proteobacteria with no close relationship to any previously described species. The novel group represented approximately 75% of the PCR-amplified DNA, based on the DGGE band intensities. Two oligonucleotide rRNA probes for this novel group were designed and used in a whole-cell hybridization analysis to investigate the abundance of this novel group in situ. The bacteria were coccoid and 3 to 4 μm in diameter and represented approximately 35% of the total population, suggesting a relatively close agreement with the results obtained by the PCR-based DGGE method. Further, based on electron microscopy and standard staining microscopic analysis, this novel group was able to accumulate granule inclusions, possibly consisting of polyhydroxyalkanoate, inside the cells.

scholarly journals AN INNOVATIVE CONTINUOUS FLOW BNR-IC PROCESS FOR NUTRIENTS REMOVAL AND PHOSPHORUS RECOVERY FROM SYNTHETIC AND REAL DOMESTIC WASTEWATER

2016 ◽  
Vol 24 (2) ◽  
pp. 116-123
Author(s):  
Haiming ZOU ◽  
Xiwu LU
Keyword(s):  
Continuous Flow ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Domestic Wastewater ◽  
Domestic Sewage ◽  
Phosphorus Recovery ◽  
Synthetic Wastewater ◽  
Nutrients Removal ◽  
Flow Process ◽  
Gradient Gel Electrophoresis ◽  
P Recovery

An innovative continuous flow process linking biological nutrients removal (BNR) with induced crystallization (IC) was used to remove nutrients and recover phosphorus (P) from synthetic and real domestic wastewater. The results showed that a good nutrients removal performance was found regardless of feeding solutions. P recovery efficiency from synthetic wastewater was 70.2% slightly less than that from real domestic sewage (74.2%). Importantly, P recovery can effectively enhance the subsequent biological P removal. Polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) analysis displayed an obvious shift in microbial community structure when switching feeding synthetic solution to real wastewater. A total of 13 bands were detected in sludge samples using synthetic and real domestic sewage, affiliated with 8 phyla or classes domain Bacteria (Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria, Flavobacteria, Actinobacteria, Sphingobacteria, Epsilonproteobacteria and Chlorobia). The results obtained here suggest that the continuous flow BNR-IC process is feasible for nutrients removal and P recovery from domestic sewage and is a promising technology for wastewater treatment combined with recycling of P elements.

scholarly journals Performance of enhanced biological phosphorus removal and population dynamics of phosphorus accumulating organisms in sludge-shifting sequencing batch reactors

2018 ◽  
Vol 78 (4) ◽  
pp. 886-895 ◽  
Author(s):  
Yang Pan ◽  
Wenquan Ruan ◽  
Yong Huang ◽  
Qianqian Chen ◽  
Hengfeng Miao ◽  
...  
Keyword(s):  
Removal Efficiency ◽  
Phosphorus Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Batch Reactor ◽  
Batch Reactors ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
High Concentration ◽  
Gradient Gel Electrophoresis ◽  
Biological Phosphorus

Abstract The sludge-shifting sequencing batch reactor (SBR) is an enhanced biological phosphorus removal (EBPR) process for wastewater treatment. In this study, the enrichment of phosphorus accumulating organisms (PAOs) will be attempted by using different high concentration of substrates. In sludge-shifting SBR, activated sludge can be continuously shifted from the bottom of SBR to anaerobic zone/selector, which contains high concentration of substrates, through an orderly reflux between the paralleled SBRs. Denaturing gradient gel electrophoresis (DGGE) methods were used to monitor microbial diversity in sludge. Fluorescence in situ hybridization (FISH) was used to determine the microbial population profile and distribution map under different sludge shifting volumes. The synthesis of intracellular polymers in this process was also analyzed. Phosphorus removal efficiency as high as 96% ± 1.3% was achieved under a sludge shifting ratio of 30%. Synthetic efficiencies of polyhydroxybutyrate (PHB) by PAOs were improved at high sludge shifting ratios. FISH results demonstrated that the population of PAOs in the process increased under properly sludge shifting ratio and it significantly improved phosphorus removal efficiency. Sequencing results indicated that determined sequences (11 OTUs) belonged to Proteobacterium, Actinobacteria and Firmicutes, Pseudomonas kuykendallii, which played an important role in the process of P removal.

Analysis of microbial community that performs enhanced biological phosphorus removal in activated sludge fed with acetate

2002 ◽  
Vol 46 (1-2) ◽  
pp. 145-154 ◽  
Author(s):  
M. Onuki ◽  
H. Satoh ◽  
T. Mino
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Phosphorus Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Batch Reactor ◽  
Phosphate Removal ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Dominant Band ◽  
Biological Phosphorus

Enhanced biological phosphorus removal (EBPR) activated sludge was operated in a laboratory-scale sequencing batch reactor (SBR) fed with acetate as the sole carbon source. The microbial community of the sludge was analyzed using the polymerase chain reaction (PCR) – denaturing gradient gel electrophoresis (DGGE) method for about 2 months of start-up period. As a result, the number of major bands decreased during the enrichment, indicating that the microbial community structure was getting simpler. Since the phosphate removal activity was maintained at a high level, the bacteria which still remained at the end can be considered as the important bacteria playing key roles in the present EBPR sludge, maybe polyphosphate accumulating organisms (PAOs). The dominant band in the last sample on the DGGE gel was excised and the DNA recovered from it was sequenced. The sequence was closely related to one of the putative PAOs group which Crocetti et al. (2000) and Hesselmann et al. (1999) have proposed. This PAOs group is closely related to the Rhodocyclus group (b-Proteobacteria). The fluorescence in situ hybridization (FISH) method with the probe specific for this PAOs group and the DAPI staining at a phosphate-probing concentration indirectly showed that these Rhodocyclus related bacteria really accumulated polyphosphate.

Role of “G-bacteria” in anaerobic substrate uptake in a SBR with no phosphorus removal

2002 ◽  
Vol 46 (1-2) ◽  
pp. 171-178 ◽  
Author(s):  
Y.H. Kong ◽  
M. Beer ◽  
R.J. Seviour ◽  
K.C. Lindrea ◽  
G.A. Rees
Keyword(s):  
16S Rdna ◽  
Phosphorus Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Phylogenetic Group ◽  
Substrate Uptake ◽  
Biological Phosphorus Removal ◽  
Gram Positive Bacteria ◽  
Gradient Gel Electrophoresis ◽  
Biological Phosphorus

Biomass from an SBR running with no enhanced biological phosphorus removal (EBPR) but which exhibited anaerobic assimilation of glucose and acetate, was dominated by “G-bacteria”, cocci in tetrads and clusters. Extracted 16S rDNA was amplified by PCR and then analysed using Denaturing Gradient Gel Electrophoresis (DGGE). Major bands were extracted and their sequences determined. Clone libraries were also prepared, the 16S rDNA extracted, PCR performed and the resultant fragments run by DGGE to aid in identifying the DGGE bands and provide fuller sequences than available by DGGE alone. The two approaches together allowed several bands to be identified. Probes for FISH analyses were designed for some of these in attempts to see to which phylogenetic group “G-bacteria” belonged, and whether they represented the dominant bands detected by DGGE. Then FISH/Microautoradiography (MAR) was used in attempts to see which bacteria there were assimilating substrates anaerobically. Results indicated that the “G-bacteria” were phylogenetically diverse, but mainly α-proteobacteria and members of the high G+C% Gram-positive bacteria. Not all of these could assimilate glucose and/or acetate anaerobically, and Amaricoccus, the original “G-bacteria” of Cech and Hartman, was not detected.

Full Scale Investigations on Enhanced Biological Phosphorus Removal – P-Release in the Anaerobic Reactor

1994 ◽  
Vol 29 (7) ◽  
pp. 153-156 ◽  
Author(s):  
D. Wedi ◽  
P. A. Wilderer
Keyword(s):  
Phosphorus Removal ◽  
Full Scale ◽  
Process Conditions ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Anaerobic Reactor ◽  
P Release ◽  
Acinetobacter Sp ◽  
Biological Phosphorus ◽  
P Removal

Most of the fundamental processes responsible for enhanced biological phosphorus removal (EBPR) were obtained through laboratory tests under defined conditions with pure or enriched cultures. Acinetobacter sp. was identified as the most important group of bacteria responsible for bio-P removal. Full scale data showed, however, that laboratory results do not match full scale results well enough. There is a lack of data on the effects of sub-optimal process conditions such as inadequate availability of volatile fatty acids (VFA), high nitrate recycle, storm water inflow or low temperatures. In this paper the results of full scale experiments on P-release are presented and compared with theoretical values. Measurements at a full scale Phoredox-system showed a surprisingly low P-release in the anaerobic reactor. Only 4 to 10% of the phosphorus in the activated sludge was released in the bulk liquid. With laboratory batch-tests, a maximum of 20% of the P in the sludge could be released. It is assumed that under the prevailing process conditions either the fraction of Acinetobacter sp. was very small, or bacteria other than Acinetobacter sp. were responsible for the P-removal, or most of the phosphorus was bound chemically but mediated by biological processes.

Bio-P and non-bio-P bacteria identification by a novel microbial approach

1999 ◽  
Vol 39 (6) ◽  
pp. 13-20 ◽  
Author(s):  
Philip L. Bond ◽  
Jürg Keller ◽  
Linda L. Blackall
Keyword(s):  
In Situ Hybridisation ◽  
Microbial Populations ◽  
Biological Phosphorus Removal ◽  
Gram Positive Bacteria ◽  
P Content ◽  
Identification Of Bacteria ◽  
Widespread Belief ◽  
Biological Phosphorus ◽  
P Removal

Culturing bacteria from activated sludge with enhanced biological phosphorus removal (EBPR) has strongly implicated Acinetobacter with the process. However, using fluorescent in-situ hybridisation (FISH) probing to analyse microbial populations, we have shown evidence opposing this widespread belief. We describe the phosphorus (P) removing performance and microbial population analyses of sludges obtained in a laboratory scale EBPR reactor. Two sludges with extremely high P removing capabilities were examined, the P content of these sludges was 8.6% (P sludge) and 12.3% (S sludge) of the MLSS. Identification of bacteria using FISH probing indicated both sludges were dominated by microbes from the beta proteobacteria and high mol% G+C Gram positive bacteria. Acinetobacter could make up only a small proportion of the cells in these sludges. Sludge with extremely poor P removal (P content of 1.5%, referred to as T sludge) was then generated by reducing the P in the influent. Bacteria resembling the G-bacteria became abundant in this sludge and these were identified using FISH probing. The anaerobic transformations of the T and P sludges correlated well with that of the non-EBPR and EBPR biological models respectively, indicating that bacteria in the T sludge have the potential to inhibit P removal in EBPR systems.

Sign in / Sign up

Export Citation Format

Share Document