Analysis of microbial community that performs enhanced biological phosphorus removal in activated sludge fed with acetate

2002 ◽  
Vol 46 (1-2) ◽  
pp. 145-154 ◽  
Author(s):  
M. Onuki ◽  
H. Satoh ◽  
T. Mino
Keyword(s):  
Microbial Community ◽  
Activated Sludge ◽  
Phosphorus Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Batch Reactor ◽  
Phosphate Removal ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Dominant Band ◽  
Biological Phosphorus

Enhanced biological phosphorus removal (EBPR) activated sludge was operated in a laboratory-scale sequencing batch reactor (SBR) fed with acetate as the sole carbon source. The microbial community of the sludge was analyzed using the polymerase chain reaction (PCR) – denaturing gradient gel electrophoresis (DGGE) method for about 2 months of start-up period. As a result, the number of major bands decreased during the enrichment, indicating that the microbial community structure was getting simpler. Since the phosphate removal activity was maintained at a high level, the bacteria which still remained at the end can be considered as the important bacteria playing key roles in the present EBPR sludge, maybe polyphosphate accumulating organisms (PAOs). The dominant band in the last sample on the DGGE gel was excised and the DNA recovered from it was sequenced. The sequence was closely related to one of the putative PAOs group which Crocetti et al. (2000) and Hesselmann et al. (1999) have proposed. This PAOs group is closely related to the Rhodocyclus group (b-Proteobacteria). The fluorescence in situ hybridization (FISH) method with the probe specific for this PAOs group and the DAPI staining at a phosphate-probing concentration indirectly showed that these Rhodocyclus related bacteria really accumulated polyphosphate.

Bacteria and Protozoa Population Dynamics in Biological Phosphate Removal Systems

1994 ◽  
Vol 29 (7) ◽  
pp. 109-117 ◽  
Author(s):  
J. S. Čech ◽  
P. Hartman ◽  
M. Macek
Keyword(s):  
Population Dynamics ◽  
Phosphorus Removal ◽  
Sequencing Batch Reactor ◽  
Batch Reactor ◽  
Sole Source ◽  
Phosphate Removal ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
System Collapse ◽  
Biological Phosphorus

Population dynamics of polyphosphate-accumulating bacteria (PP bacteria) was studied in a laboratory sequencing batch reactor simulating anaerobic-oxic sludge system. The competition between PP bacteria and another microorganism (“G bacteria”) for anaerobic-oxic utilization of acetate as the sole source of organic carbon was observed. The competition was found to be seriously influenced by protozoan and metazoan grazing: Predation-resistant “G bacteria” forming large compact flocs outcompeted PP bacteria. Several breakdowns of enhanced biological phosphorus removal were observed. The first one was related to the development of an euglenid flagellate Entosiphon sulcatus and attached ciliates Vorticella microstoma and V. campanula. The second system collapse was connected with a rapid proliferation of rotifers. An alternative-prey predation was thought to be a mechanism of PP bacteria elimination.

scholarly journals Performance of enhanced biological phosphorus removal and population dynamics of phosphorus accumulating organisms in sludge-shifting sequencing batch reactors

2018 ◽  
Vol 78 (4) ◽  
pp. 886-895 ◽  
Author(s):  
Yang Pan ◽  
Wenquan Ruan ◽  
Yong Huang ◽  
Qianqian Chen ◽  
Hengfeng Miao ◽  
...  
Keyword(s):  
Removal Efficiency ◽  
Phosphorus Removal ◽  
Denaturing Gradient Gel Electrophoresis ◽  
Batch Reactor ◽  
Batch Reactors ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
High Concentration ◽  
Gradient Gel Electrophoresis ◽  
Biological Phosphorus

Abstract The sludge-shifting sequencing batch reactor (SBR) is an enhanced biological phosphorus removal (EBPR) process for wastewater treatment. In this study, the enrichment of phosphorus accumulating organisms (PAOs) will be attempted by using different high concentration of substrates. In sludge-shifting SBR, activated sludge can be continuously shifted from the bottom of SBR to anaerobic zone/selector, which contains high concentration of substrates, through an orderly reflux between the paralleled SBRs. Denaturing gradient gel electrophoresis (DGGE) methods were used to monitor microbial diversity in sludge. Fluorescence in situ hybridization (FISH) was used to determine the microbial population profile and distribution map under different sludge shifting volumes. The synthesis of intracellular polymers in this process was also analyzed. Phosphorus removal efficiency as high as 96% ± 1.3% was achieved under a sludge shifting ratio of 30%. Synthetic efficiencies of polyhydroxybutyrate (PHB) by PAOs were improved at high sludge shifting ratios. FISH results demonstrated that the population of PAOs in the process increased under properly sludge shifting ratio and it significantly improved phosphorus removal efficiency. Sequencing results indicated that determined sequences (11 OTUs) belonged to Proteobacterium, Actinobacteria and Firmicutes, Pseudomonas kuykendallii, which played an important role in the process of P removal.

The Influence of Nitrogen on Phosphorus Removal in Activated Sludge Plants

1982 ◽  
Vol 14 (1-2) ◽  
pp. 31-45 ◽  
Author(s):  
J L Barnard
Keyword(s):  
Organic Carbon ◽  
Activated Sludge ◽  
Phosphorus Removal ◽  
Complete Removal ◽  
Phosphate Removal ◽  
Electron Donors ◽  
Biological Phosphorus Removal ◽  
Anaerobic Conditions ◽  
Low Level ◽  
Biological Phosphorus

This paper discusses the need for anaerobiases as a pre-requisite for phosphorus removal in activated sludge plants and the effect of nitrates on the anaerobic conditions. If the plant could be operated to avoid nitrification, biological phosphorus removal presents no problems. When nitrification is required, the nitrates must be reduced to a low level through internal denitrification. If sufficient carbon is available to ensure complete removal of the nitrates and anaerobic conditions in a specific zone in the plant, good phosphate removal can be ensured. Below COD : TKN ratios of 10 : 1 it is becoming more difficult to control the plant and special care should be taken to determine not only the quantity of organic carbon available as electron donors for removal of the nitrates but also the form in which it arrives at the plant.

Biochemical Aspects of Enhanced Biological Phosphorus Removal from Wastewater

1985 ◽  
Vol 17 (11-12) ◽  
pp. 23-41 ◽  
Author(s):  
M. C. Hascoet ◽  
M. Florentz ◽  
P. Granger
Keyword(s):  
Activated Sludge ◽  
Phosphorus Removal ◽  
Phosphorus Uptake ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
The Past ◽  
Nitrate Addition ◽  
The Subject ◽  
Biological Phosphorus ◽  
Microbiological Analyses

Enhanced biological phosphorus removal from wastewater by means of microorganisms found in activated sludge has for the past few years been the subject of much research and it is now commonly recognized that an activated sludge system must include alternating anaerobic-aerobic periods. The present article covers biochemical aspects of this phenomenon using a phosphorus removing biomass obtained in a laboratory-scale pilot with alternating phases and a synthetic substrate feed. The percentage of phosphorus obtained in the pilot sludge was four times greater than that of a conventional sludge plant. By exposing the same biomass to different conditions and using 31P Nuclear Magnetic Resonance, we were able to accurately pin-point the various forms of phosphorus found within cells and follow their development during the course of alternating phases. The following results were obtained:the transformation of phosphorus in its inorganic to polyphosphate form depends on the medium's level of oxygenation,the presence of nitrates disturbs the anaerobic period but does not affect phosphorus uptake in the aerated period.Continuous nitrate addition alters biomass behaviour in the anaerobic phase, which loses the capacity to release phosphorus,copper at a concentration of over 1 mg Cu2+/1 inhibits phosphorus uptake in the aerated phase. Various microbiological analyses made on the pilot biomass isolated conventional bacteria found in activated sludge.

An enhanced biological phosphorus removal (EBPR) control strategy for sequencing batch reactors (SBRs)

2001 ◽  
Vol 43 (3) ◽  
pp. 183-189 ◽  
Author(s):  
C. Y. Dassanayake ◽  
R. L. Irvine
Keyword(s):  
Control Strategy ◽  
Phosphorus Removal ◽  
Batch Reactor ◽  
Past Research ◽  
P Uptake ◽  
Batch Reactors ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
Aeration Time ◽  
Biological Phosphorus

A control strategy was developed for enhanced biological phosphorus removal (EBPR) in a Sequencing Batch Reactor (SBR). Unlike past research that focused on maximizing polyhdroxyalkanoate (PHA) formation during the anaerobic period, this study investigated some of the factors that govern aerobic PHA dynamics and its efficient regulation during phosphate (P) uptake. Influent COD, influent P, and the time for aeration were critical factors that governed PHA use and P uptake during aerated react. Unnecessary PHA oxidation (i.e., in the absence of extracellular P) occurred if the time for aerated react exceeded the time required for P uptake. By adjusting the aeration time to that required for P uptake, residual PHA was sustained in the SBR and excess phosphate uptake reaction potential (PRP) was generated for use during transient influent excursions in P. Unlike space oriented systems, the time for react is simply adjusted in the SBR. Because residual PHA is easily maintained once achieved, high influent COD events can be harnessed to increase or sustain excess PRP for management of expected variations in influent P.

Design of nutrient removal activated sludge systems

2003 ◽  
Vol 47 (11) ◽  
pp. 115-122 ◽  
Author(s):  
J. Manga ◽  
J. Ferrer ◽  
A. Seco ◽  
F. Garcia-Usach
Keyword(s):  
Mathematical Model ◽  
Activated Sludge ◽  
Nutrient Removal ◽  
Phosphorus Removal ◽  
Pilot Plant ◽  
Enhanced Biological Phosphorus Removal ◽  
Biological Phosphorus Removal ◽  
User Friendly ◽  
Biological Phosphorus ◽  
Activated Sludge Systems

A mechanistic mathematical model for nutrient and organic matter removal was used to describe the behavior of a nitrification denitrification enhanced biological phosphorus removal (NDEBPR) system. This model was implemented in a user-friendly software DESASS (design and simulation of activated sludge systems). A 484-L pilot plant was operated to verify the model results. The pilot plant was operated for three years over three different sludge ages. The validity of the model was confirmed with data from the pilot plant. Also, the utility of DESASS as a valuable tool for designing NDEBPR systems was confirmed.

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