Selenium recovery from kiln powder of cement manufacturing by chemical leaching and bioreduction

2015 ◽  
Vol 72 (8) ◽  
pp. 1294-1300 ◽  
Author(s):  
S. Soda ◽  
A. Hasegawa ◽  
M. Kuroda ◽  
A. Hanada ◽  
M. Yamashita ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Pseudomonas Stutzeri ◽  
Treated Wastewater ◽  
Elemental Selenium ◽  
Chemical Leaching ◽  
Slurry Concentration ◽  
Effluent Concentration ◽  
Cement Manufacturing

A novel process by using chemical leaching followed by bacterial reductive precipitation was proposed for selenium recovery from kiln powder as a byproduct of cement manufacturing. The kiln powder at a slurry concentration of 10 w/v% with 0.25 M Na2CO3 at 28°C produced wastewater containing about 30 mg-Se/L selenium. The wastewater was diluted four-fold and adjusted to pH 8.0 as preconditioning for bioreduction. A bacterial strain Pseudomonas stutzeri NT-I, capable of reducing selenate and selenite into insoluble elemental selenium, could recover about 90% selenium from the preconditioned wastewater containing selenium of 5 mg-Se/L when supplemented with lactate or glycerol. The selenium concentrations in the treated wastewater were low around the regulated effluent concentration of 0.1 mg-Se/L in Japan.

scholarly journals Pyridine-2,6-Bis(Thiocarboxylic Acid) Produced by Pseudomonas stutzeri KC Reduces and Precipitates Selenium and Tellurium Oxyanions

2006 ◽  
Vol 72 (5) ◽  
pp. 3119-3129 ◽  
Author(s):  
Anna M. Zawadzka ◽  
Ronald L. Crawford ◽  
Andrzej J. Paszczynski
Keyword(s):  
Wild Type Strain ◽  
Hydrolysis Product ◽  
Pseudomonas Stutzeri ◽  
Elemental Selenium ◽  
Bacterial Cells ◽  
Wild Type ◽  
Chemical Structures ◽  
Thiocarboxylic Acid ◽  
Insoluble Compounds ◽  
Initial Line

ABSTRACT The siderophore of Pseudomonas stutzeri KC, pyridine-2,6-bis(thiocarboxylic acid) (pdtc), is shown to detoxify selenium and tellurium oxyanions in bacterial cultures. A mechanism for pdtc's detoxification of tellurite and selenite is proposed. The mechanism is based upon determination using mass spectrometry and energy-dispersive X-ray spectrometry of the chemical structures of compounds formed during initial reactions of tellurite and selenite with pdtc. Selenite and tellurite are reduced by pdtc or its hydrolysis product H2S, forming zero-valent pdtc selenides and pdtc tellurides that precipitate from solution. These insoluble compounds then hydrolyze, releasing nanometer-sized particles of elemental selenium or tellurium. Electron microscopy studies showed both extracellular precipitation and internal deposition of these metalloids by bacterial cells. The precipitates formed with synthetic pdtc were similar to those formed in pdtc-producing cultures of P. stutzeri KC. Culture filtrates of P. stutzeri KC containing pdtc were also active in removing selenite and precipitating elemental selenium and tellurium. The pdtc-producing wild-type strain KC conferred higher tolerance against selenite and tellurite toxicity than a pdtc-negative mutant strain, CTN1. These observations support the hypothesis that pdtc not only functions as a siderophore but also is involved in an initial line of defense against toxicity from various metals and metalloids.

scholarly journals Identification of Anaerobic Selenate-Respiring Bacteria from Aquatic Sediments

2007 ◽  
Vol 73 (11) ◽  
pp. 3519-3527 ◽  
Author(s):  
Priya Narasingarao ◽  
Max M. Häggblom
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Anaerobic Respiration ◽  
Pseudomonas Stutzeri ◽  
Elemental Selenium ◽  
Rrna Gene ◽  
Terminal Electron Acceptor ◽  
Isolation And Characterization ◽  
Anaerobic Biotransformation ◽  
Gene Similarity

ABSTRACT The diversity population of microorganisms with the capability to use selenate as a terminal electron acceptor, reducing it to selenite and elemental selenium by the process known as dissimilatory selenate reduction, is largely unknown. The overall objective of this study was to gain an in-depth understanding of anaerobic biotransformation of selenium in the environment, particularly anaerobic respiration, and to characterize the microorganisms catalyzing this process. Here, we demonstrate the isolation and characterization of four novel anaerobic dissimilatory selenate-respiring bacteria enriched from a variety of sources, including sediments from three different water bodies in Chennai, India, and a tidal estuary in New Jersey. Strains S5 and S7 from India, strain KM from the Meadowlands, NJ, and strain pn1, categorized as a laboratory contaminant, were all phylogenetically distinct, belonging to various phyla in the bacterial domain. The 16S rRNA gene sequence shows that strain S5 constitutes a new genus belonging to Chrysiogenetes, while strain S7 belongs to the Deferribacteres, with greater than 98% 16S rRNA gene similarity to Geovibrio ferrireducens. Strain KM is related to Malonomonas rubra, Pelobacter acidigallici, and Desulfuromusa spp., with 96 to 97% 16S rRNA gene similarity. Strain pn1 is 99% similar to Pseudomonas stutzeri. Strains S5, S7, and KM are obligately anaerobic selenate-respiring microorganisms, while strain pn1 is facultatively anaerobic. Besides respiring selenate, all these strains also respire nitrate.

scholarly journals Biodegradation of Alprazolam in Pharmaceutical Wastewater Using Mesoporous Nanoparticles-Adhered Pseudomonas stutzeri

Molecules ◽  
2021 ◽  
Vol 27 (1) ◽  
pp. 237
Author(s):  
Mahdi Shahriarinour ◽  
Faten Divsar ◽  
Fereshteh Kamalpour Dahka ◽  
Sharareh Nezamivand Chegini ◽  
Mohamad Mahani ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Mesoporous Silica Nanoparticles ◽  
Pseudomonas Stutzeri ◽  
Degradation Process ◽  
Inorganic Nanoparticles ◽  
Bacterial Cells ◽  
Pharmaceutical Wastewater ◽  
Bacterial Immobilization ◽  
Freely Suspended ◽  
Quantitative Hplc Analysis

The release of pharmaceutical wastewaters in the environment is of great concern due to the presence of persistent organic pollutants with toxic effects on environment and human health. Treatment of these wastewaters with microorganisms has gained increasing attention, as they can efficiently biodegrade and remove contaminants from the aqueous environments. In this respect, bacterial immobilization with inorganic nanoparticles provides a number of advantages, in terms of ease of processing, increased concentration of the pollutant in proximity of the cell surface, and long-term reusability. In the present study, MCM-41 mesoporous silica nanoparticles (MSN) were immobilized on a selected bacterial strain to remove alprazolam, a persistent pharmaceutical compound, from contaminated water. First, biodegrading microorganisms were collected from pharmaceutical wastewater, and Pseudomonas stutzeri was isolated as a bacterial strain showing high ability to tolerate and consume alprazolam as the only source for carbon and energy. Then, the ability of MSN-adhered Pseudomonas stutzeri bacteria was assessed to biodegrade alprazolam using quantitative HPLC analysis. The results indicated that after 20 days in optimum conditions, MSN-adhered bacterial cells achieved 96% biodegradation efficiency in comparison to the 87% biodegradation ability of Pseudomonas stutzeri freely suspended cells. Kinetic study showed that the degradation process obeys a first order reaction. In addition, the kinetic constants for the MSN-adhered bacteria were higher than those of the bacteria alone.

scholarly journals Stenotrophomonas maltophilia SeITE02, a New Bacterial Strain Suitable for Bioremediation of Selenite-Contaminated Environmental Matrices

2007 ◽  
Vol 73 (21) ◽  
pp. 6854-6863 ◽  
Author(s):  
Paolo Antonioli ◽  
Silvia Lampis ◽  
Irene Chesini ◽  
Giovanni Vallini ◽  
Sara Rinalducci ◽  
...  
Keyword(s):  
Bacterial Strain ◽  
Stenotrophomonas Maltophilia ◽  
Maximum Activity ◽  
Mass Spectrometry Analysis ◽  
Elemental Selenium ◽  
Cellular Transport ◽  
Environmental Matrices ◽  
Nucleotide Synthesis ◽  
Selenite Reduction

ABSTRACT Biochemical and proteomic tools have been utilized for investigating the mechanism of action of a new Stenotrophomonas maltophilia strain (SeITE02), a gammaproteobacterium capable of resistance to high concentrations of selenite [SeO3 2−, Se(IV)], reducing it to nontoxic elemental selenium under aerobic conditions; this strain was previously isolated from a selenite-contaminated mining soil. Biochemical analysis demonstrated that (i) nitrite reductase does not seem to take part in the process of selenite reduction by the bacterial strain SeITE02, although its involvement in this process had been hypothesized in other cases; (ii) nitrite strongly interferes with selenite removal when the two oxyanions (NO2 − and SeO3 2−) are simultaneously present, suggesting that the two reduction/detoxification pathways share a common enzymatic step, probably at the level of cellular transport; (iii) in vitro, selenite reduction does not take place in the membrane or periplasmic fractions but only in the cytoplasm, where maximum activity is exhibited at pH 6.0 in the presence of NADPH; and (iv) glutathione is involved in the selenite reduction mechanism, since inhibition of its synthesis leads to a considerable delay in the onset of reduction. As far as the proteomic findings are concerned, the evidence was reached that 0.2 mM selenite and 16 mM nitrite, when added to the culture medium, caused a significant modulation (ca. 10%, i.e., 96 and 85 protein zones, respectively) of the total proteins visualized in the respective two-dimensional maps. These spots were identified by mass spectrometry analysis and were found to belong to the following functional classes: nucleotide synthesis and metabolism, damaged-protein catabolism, protein and amino acid metabolism, and carbohydrate metabolism along with DNA-related proteins and proteins involved in cell division, oxidative stress, and cell wall synthesis.

scholarly journals Reduction of Selenate and Selenite to Elemental Selenium by a Pseudomonas stutzeri Isolate

1992 ◽  
Vol 58 (12) ◽  
pp. 4042-4044 ◽  
Author(s):  
L. Lortie ◽  
W. D. Gould ◽  
S. Rajan ◽  
R. G. L. McCready ◽  
K.-J. Cheng
Keyword(s):  
Pseudomonas Stutzeri ◽  
Elemental Selenium

scholarly journals Biodegradation of Methyl Parathion Using Pseudomonas stutzeri (MTCC 2643)

2020 ◽  
Vol 2 (2) ◽  
pp. 19-33
Author(s):  
Archana S ◽  
Surendran A ◽  
Thatheyus AJ
Keyword(s):  
Bacterial Strain ◽  
Hplc Analysis ◽  
Methyl Parathion ◽  
Crop Protection ◽  
Pseudomonas Stutzeri ◽  
Cost Effective ◽  
Agricultural Fields ◽  
Microbial Remediation ◽  
Uv Visible

Pesticides applied in agricultural fields for crop protection result in the contamination of the environment. They also affect the flora and fauna as well as the quality of air, water and soil. Hence their remediation is of concern. Among different strategies available, microbial remediation is cost effective and ecofriendly. The present work is designed to test the efficiency of Pseudomonas stutzeri, a bacterial strain obtained from MTCC, IMTECH, Chandigarh, India. After treating with 50, 100, 150 and 200 ppm of methyl parathion, parameters like, orthophosphate released, pH and turbidity changes were monitored upto thirty hours. Degradation of 200 ppm concentration was confirmed by UV-Visible spectrophotometry and HPLC analysis. This strain effectively degraded methyl parathion and it can be used in the remediation of contaminated agricultural fields.

DECOLOURIZATION AND COD REMOVAL OF WASEWATER FROM ETHANOL PRODUCTION PROCESS FROM MOLASSES BY COAGULANTION USING INORGANIC ALUM

2010 ◽  
Vol 13 (3) ◽  
pp. 92-102
Author(s):  
Trung Duc Le
Keyword(s):  
Ethanol Production ◽  
Oxygen Demand ◽  
Cod Removal ◽  
Treated Wastewater ◽  
Treatment Processes ◽  
Physico Chemical ◽  
Biological Methods ◽  
Treatment Step ◽  
Main Material

The industrial production of ethanol by fermentation using molasses as main material that generates large quantity of wastewater. This wastewater contains high levels of colour and chemical oxygen demand (COD), that may causes serious environmental pollution. Most available treatment processes in Vietnam rely on biological methods, which often fail to treat waste water up to discharge standard. As always, it was reported that quality of treated wastewater could not meet Vietnameses discharge standard. So, it is necessary to improve the treatment efficiency of whole technological process and therefore, supplemental physico-chemical treatment step before biodegradation stage should be the appropriate choice. This study was carried out to assess the effect of coagulation process on decolourization and COD removal in molasses-based ethanol production wastewater using inorganic coaglutant under laboratory conditions. The experimental results showed that the reductions of COD and colour with the utilization of Al2(SO4)3 at pH 9.5 were 83% and 70%, respectively. Mixture FeSO4 – Al2(SO4)3 at pH 8.5 reduced 82% of colour and 70% of COD. With the addition of Polyacrylamide (PAM), the reduction efficiencies of colour, COD and turbidity by FeSO4 – Al2(SO4)3 were 87%, 73.1% and 94.1% correspondingly. It was indicated that PAM significantly reduced the turbidity of wastewater, however it virtually did not increase the efficiencies of colour and COD reduction. Furthermore, the coagulation processes using PAM usually produces a mount of sludge which is hard to be deposited.

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