Copaiba oil-resin (Copaifera langsdorfii Desf.: Caesalpiniaceae) associated with laser therapy for skin wound treatment in Wistar rats

Alternative protocols for the treatment of skin lesions have been developed with the use of techniques such as photobiomodulation and phytotherapy, aiming to optimize this process. To evaluate the effectiveness of copaiba (Copaiferalangsdorffii) oil-resin and low-level laser therapy for treating cutaneous wounds, 15 Wistar rats (Rattusnorvergicus) were used, in whom five 8-mm lesions were produced. The following protocols were applied: negative control group (T1); positive control group (T2); laser therapy with AsGa (904 nm), continuous, focal mode for 10 s, dosage of 4 J/cm² (T3); copaiba oil-resin (T4); and association group (copaiba and low-level laser) (T5). The efficacy of each technique was evaluated based on macroscopic aspects of the lesion, wound healing rate, and histopathological analysis (inflammatory infiltrate and collagen expression). The Kruskal-Wallis test was used for statistical analyses (P> 0.05). Copaiba treatment showed an advantage in type III collagen expression, whereas laser therapy demonstrated an enhanced capacity for tissue regeneration. The significant advantage obtained from the association treatment is the improvement of the macroscopic aspect of the wound, with a reduction in crust formation.

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Low-level laser therapy on the rat’s gastrocnemius morphometry submitted to a rheumatoid arthritis model

European Journal of Clinical and Experimental Medicine ◽

10.15584/ejcem.2021.2.1 ◽

2021 ◽

Vol 19 (2) ◽

pp. 111-115

Author(s):

Estéfani Marin ◽

◽

Jacqueline Lumy Fuse ◽

Larissa Pereira Lopes ◽

Morgana Neves ◽

...

Keyword(s):

Rheumatoid Arthritis ◽

Laser Therapy ◽

Wistar Rats ◽

Low Level Laser Therapy ◽

Lesion Group ◽

Control Group ◽

Low Level ◽

Low Level Laser ◽

Level Laser ◽

Significant Difference

Introduction. Rheumatoid arthritis (RA) is a chronic, systemic, autoimmune inflammatory disease of unknown origin, mainly affecting synovial joints and related structures, including the adjacent musculature, generating great disability and reduction in quality of life. Aim. This study was designed to investigate the effect of low-level laser therapy (LLLT) on gastrocnemius of Wistar rats subjected to an experimental model of RA. Material and methods. Forty male Wistar rats were used, separated into: acute and chronic, being subdivided into Control Group (CG): without intervention, Lesion Group (LG): submitted to lesion, Laser Control Group (LCG): without lesion and with treatment, and Laser Lesion Group (LLG): submitted to lesion and LLLT. The treatment with LLLT occurred in four points of the right knee, wavelength of 660 nm, energy density of 5 J/cm2, energy per point of 0.003 J. Morphometric analysis was performed using a 40x magnification photomicrograph and analyzed using the Image-Pro-Plus 6.0 program. Results. As result of the acute group there was a difference only for muscle mass, being higher in CG. For the chronic group there was significant difference for cross-sectional area, larger and smaller diameter, again with the control group obtaining higher values than the others, for the number of nuclei LG was lower than CG and LCG, but LLG was not different from any of them. Conclusion. It is concluded that treatment with LLLT was not very effective in reversing the harmful effects of RA on the gastrocnemius muscle.

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Assessment of healing of dental implant surgical site following Low-Level Laser Therapy using Bio-Clinical parameters: An Exploratory Study

Journal of Oral Implantology ◽

10.1563/aaid-joi-d-18-00316 ◽

2020 ◽

Author(s):

VARSHA PALLED ◽

DR. JITENDRA RAO ◽

DR. RAGHUWAR DAYAL SINGH ◽

DR. SHUCHI TRIPATHI ◽

DR. KALPANA SINGH ◽

...

Keyword(s):

Laser Therapy ◽

Test Group ◽

Low Level Laser Therapy ◽

Control Group ◽

Clinical Parameters ◽

Implant Stability ◽

Low Level ◽

Implant Stability Quotient ◽

Low Level Laser ◽

Level Laser

The purpose of this study was to evaluate whether Low-Level Laser Therapy (LLLT) improves the healing of the implant surgical site with clinical and biochemical parameters.Thirty patients with an edentulous space spanning a single tooth were selected. The patients were randomly allocated to two groups - the control group and the test group. The test group received laser energy at a power of 2J/cm 2 with a total of 4-6J energy over each implant. Clinical parameters (Implant Stability Quotient, probing index, modified sulcus bleeding index)and osteoprotegerin (OPG) were assessed at baseline and follow-up intervals (2 weeks, 6 weeks, and 3 months).The test group showed significantly higher implant stability quotient compared to thecontrol group at 2 weeks(57.93±3.95 and 35.67±3.08; p<0.01) and 3months(58.86±3.75 and 67.06±3.78; p<0.01). A significant rise in OPG levels of the test group(686.30±125.36pg/ml at baseline and 784.25±108.30pg/ml at 3months;p<0.01) was seen contrary to significant decline in the control group (839.50±249.08pg/ml at baseline,415.30±78.39pg/ml at 3months;p<0.01). Within the limitations of the study, the study suggests that the healing of peri-implant hard and soft tissues may be enhanced with the use of LLLT as an explicit modality during the post-operative period.

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Effects of Photobiomodulation with Low‐level Laser Therapy on Muscle Repair Following a Peripheral Nerve Injury in Wistar Rats

Photochemistry and Photobiology ◽

10.1111/php.13255 ◽

2020 ◽

Vol 96 (5) ◽

pp. 1124-1132

Author(s):

Lucas Andreo ◽

Beatriz G. Ribeiro ◽

Agnelo N. Alves ◽

Andréia S.A. Martinelli ◽

Carla B. Soldera ◽

...

Keyword(s):

Peripheral Nerve ◽

Laser Therapy ◽

Nerve Injury ◽

Peripheral Nerve Injury ◽

Wistar Rats ◽

Low Level Laser Therapy ◽

Muscle Repair ◽

Low Level ◽

Low Level Laser ◽

Level Laser

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Effects of Low-Level Laser Therapy Applied Before Treadmill Training on Recovery of Injured Skeletal Muscle in Wistar Rats

Photomedicine and Laser Surgery ◽

10.1089/pho.2015.4031 ◽

2016 ◽

Vol 34 (5) ◽

pp. 187-193 ◽

Cited By ~ 7

Author(s):

Mayna Adabbo ◽

Fernanda Rossi Paolillo ◽

Paulo Sérgio Bossini ◽

Natalia Camargo Rodrigues ◽

Vanderlei Salvador Bagnato ◽

...

Keyword(s):

Skeletal Muscle ◽

Laser Therapy ◽

Wistar Rats ◽

Treadmill Training ◽

Low Level Laser Therapy ◽

Low Level ◽

Low Level Laser ◽

Level Laser

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ASSESSMENT ON RESULTS OF SURGICAL EXTRACTION OF&NBSP; IMPACTED LOWER THIRD MOLARS WITH POSTOPERATIVE LOW - LEVEL LASER THERAPY

Journal of Medicine and Pharmacy ◽

10.34071/jmp.2018.6.17 ◽

2018 ◽

Vol 8 (6) ◽

pp. 130-137

Author(s):

Huong Nguyen Thi Mai ◽

Tai Tran Tan ◽

Khanh Hong Quoc

Keyword(s):

Laser Therapy ◽

Third Molar ◽

Low Level Laser Therapy ◽

Control Group ◽

Low Level ◽

Impacted Third Molar ◽

Low Level Laser ◽

Level Laser ◽

Surgical Extraction ◽

Wisdom Teeth

Background: The most frequently performed surgical procedure in dentistry is impacted third molar extraction with difficulty varies according to the location of the tooth. Laser therapy after surgery can accelerate cell and tissue reconstruction along with relieve post-operative pain. The objective of this study was to investigate the clinical and radiographic characteristics of impacted lower third molar and to evaluate the results of surgical extraction of impacted lower third molar with post-surgical low-level laser therapy (LLLT). Subjects and Methods: Clinical and radiographic data from 90 patients (average age 28.13 ± 5.38) subjected to a surgical extraction of lower third molar were pooled and divided randomly into 3 groups: group 1 received LLLT immediately after surgery intraorally, group 2 treated with LLLT immediately after the extraction extraorally. Patients received routine management with nonactivated laser were inserted in the control group. Assessments of pain, swelling and trismus level were carried out at 24, 48 hours and on the 7th day after surgery. Results: Correlation of wisdom teeth to ramus and adjacent teeth mainly type II (88.9%), type III accounted for 11.1%. Relative depth of wisdom teeth in the bone mainly position B (81.1%), position C (18.9%). Correlation of wisdom teeth axis to adjacent teeth: horizontal (58.9%), mesioangular impactions (40%) and distoangular impaction (1.1%). There were statistically significant decreases in the level of pain, edema and interincisal opening between the laser-treated groups and the control group on the 1st and 2nd postoperative day. Conclusions: Intraoral and extraoral post-surgical low-level laser therapy treatment was useful in reducing swelling, pain and trismus compared to placebo group in impacted third molar surgery. Key words: Impacted lower third molar, low- level laser therapy

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Application of a Low-Level Laser Therapy and the Purified Protein from Natural Latex (Hevea brasiliensis) in the Controlled Crush Injury of the Sciatic Nerve of Rats: A Morphological, Quantitative, and Ultrastructural Study

BioMed Research International ◽

10.1155/2013/597863 ◽

2013 ◽

Vol 2013 ◽

pp. 1-11 ◽

Cited By ~ 5

Author(s):

Fernando José Dias ◽

João Paulo Mardegan Issa ◽

Mamie Mizusaki Iyomasa ◽

Joaquim Coutinho-Netto ◽

Ricardo Alexandre Junqueria Calzzani ◽

...

Keyword(s):

Sciatic Nerve ◽

Laser Therapy ◽

Ultrastructural Analysis ◽

Crush Injury ◽

Low Level Laser Therapy ◽

Control Group ◽

Low Level ◽

Injured Nerve ◽

Low Level Laser ◽

Level Laser

This study analyzed the effects of a low-level laser therapy (LLLT, 15 J/cm2, 780 nm wavelength) and the natural latex protein (P1, 0.1%) in sciatic nerve after crush injury (15 Kgf, axonotmesis) in rats. Sixty rats (male, 250 g) were allocated into the 6 groups (n=10): CG—control group; EG—nerve exposed; IG—injured nerve without treatment; LG—crushed nerve treated with LLLT; PG—injured nerve treated with P1; and LPG—injured nerve treated with LLLT and P1. After 4 or 8 weeks, the nerve samples were processed for morphological, histological quantification and ultrastructural analysis. After 4 weeks, the myelin density and morphological characteristics improved in groups LG, PG, and LPG compared to IG. After 8 weeks, PG, and LPG were similar to CG and the capillary density was higher in the LG, PG, and LPG. In the ultrastructural analysis the PG and LPG had characteristics that were similar to the CG. The application of LLLT and/or P1 improved the recovery from the nerve crush injury, and in the long term, the P1 protein was the better treatment used, since only the application of LLLT has not reached the same results, and these treatments applied together did not potentiate the recovery.

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Equine wound healing: influence of low level laser therapy on an equine metacarpal wound healing model

Photonics and Lasers in Medicine ◽

10.1515/plm-2012-0004 ◽

2012 ◽

Vol 1 (2) ◽

Cited By ~ 8

Author(s):

Henry W. Jann ◽

Kenneth Bartels ◽

Jerry W. Ritchey ◽

Mark Payton ◽

John M. Bennett

Keyword(s):

Wound Healing ◽

Laser Therapy ◽

Low Level Laser Therapy ◽

Energy Output ◽

Control Group ◽

Skin Wounds ◽

Full Thickness ◽

Low Level ◽

Low Level Laser ◽

Level Laser

AbstractTo evaluate the effects of low level laser therapy (LLLT) on healing of full thickness symmetrical skin wounds in horses. LLLT is a therapeutic modality using the application of light, usually a low power laser or light emitting diode in the power range of 1 mW to 12 W that, in practical terms, promotes tissue regeneration as well as reducing inflammation and pain.Experimental study.Healthy horses (n=8).Full thickness, 2.5 cm square skin wounds were created in the mid-metacarpal region on one leg of eight normal horses. LLLT was used on limbs assigned to the experimental group and limbs assigned to the control group were allowed to heal without treatment. LLLT was administered using a line generated optical scanner with a dual diode laser system (model EML; Erchonia Laser Healthcare, McKinney, TX, USA) at a wavelength of 635 nm and an energy output of 17 mW per diode. Wound size was measured for an 80-day period post operatively. Eighty days after surgery incisional biopsies were examined histologically.Wounds treated with LLLT healed faster than the control wounds (LLLT increased the rate of wound healing.

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Effects of low-level laser therapy on collagen expression and neutrophil infiltrate in 5-fluorouracil-induced oral mucositis in hamsters

Lasers in Surgery and Medicine ◽

10.1002/lsm.20920 ◽

2010 ◽

Vol 42 (6) ◽

pp. 546-552 ◽

Cited By ~ 43

Author(s):

Nilza Nelly Fontana Lopes ◽

Hélio Plapler ◽

Rajesh V. Lalla ◽

Maria Cristina Chavantes ◽

Elisabeth Mateus Yoshimura ◽

...

Keyword(s):

Laser Therapy ◽

Oral Mucositis ◽

Low Level Laser Therapy ◽

Low Level ◽

Collagen Expression ◽

Low Level Laser ◽

Level Laser ◽

Neutrophil Infiltrate

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Effects of low-level laser therapy on cartilage repair in an experimental model of osteoarthritis

Photonics and Lasers in Medicine ◽

10.1515/plm-2013-0063 ◽

2014 ◽

Vol 3 (3) ◽

Author(s):

Anderson Amaro dos Santos ◽

Poliani Oliveira ◽

Kelly Rossetti Fernandes ◽

Lara Rhon ◽

Carla Roberta Tim ◽

...

Keyword(s):

Laser Therapy ◽

Cruciate Ligament ◽

Treated Group ◽

Male Rats ◽

Low Level Laser Therapy ◽

Control Group ◽

Low Level ◽

Low Level Laser ◽

Post Surgery ◽

Level Laser

AbstractObjective:The aim of this study was to evaluate the effects of low level laser therapy (LLLT) on the degenerative process in the articular cartilage after an anterior cruciate ligament transection (ACLT) model in rats.Methods:Eighty male rats (Wistar) were divided into four groups: 1.) intact control group (CG), 2.) injured control group (ICG), 3.) injured laser-treated group at 10 J/cmResults:Initial signs of tissue degradation could be observed 5 weeks post-ACLT, evidenced by the decrease of proteoglycan concentration and increase in cartilage thickness of the ICG. After 8 weeks post-surgery, analysis showed a progression of the degenerative processes in the ICG revealed by the increased cellularity and higher TNF-α, IL1-β and MMP-13 immunoexpression. LLLT was able to modulate some of the aspects relating to the degradative process, such as biomodulation of the number of chondrocyte proliferation, prevention of proteoglycan loss, and decrease of MMP-13 immunoexpression.Conclusion:This study showed that the 685-nm laser irradiation, especially at 10 J/cm

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