scholarly journals Improved maceral separation method using a continuous flow centrifuge

1994 ◽  
Author(s):  
G.R. Dyrkacz ◽  
C.A.A. Bloomquist
Keyword(s):  
Continuous Flow ◽  
Separation Method ◽  
Continuous Flow Centrifuge

The Role of Continuous Flow Centrifuge Leucapheresis in the Management of Chronic Lymphocytic Leukaemia

1976 ◽  
Vol 32 (4) ◽  
pp. 609-616 ◽  
Author(s):  
I. E. Fortuny ◽  
D. C. Hadlock ◽  
B. J. Kennedy ◽  
A. Theologides ◽  
J. McCullough
Keyword(s):  
Chronic Lymphocytic Leukaemia ◽  
Continuous Flow ◽  
Lymphocytic Leukaemia ◽  
Continuous Flow Centrifuge

Colony-forming cell assay for human hematopoietic progenitor cells harvested by a novel continuous-flow cell separation method

2007 ◽  
Vol 1151 (1-2) ◽  
pp. 153-157 ◽  
Author(s):  
Hiroyuki Shiono ◽  
Hong Miao Chen ◽  
Tadashi Okada ◽  
Yoichiro Ito
Keyword(s):  
Progenitor Cells ◽  
Continuous Flow ◽  
Cell Separation ◽  
Flow Cell ◽  
Separation Method ◽  
Hematopoietic Progenitor Cells ◽  
Hematopoietic Progenitor ◽  
Cell Assay ◽  
Colony Forming Cell

scholarly journals Increased Efficiency of Leukocyte Collection by the Addition of Hydroxyethyl Starch to the Continuous Flow Centrifuge

Blood ◽  
1974 ◽  
Vol 44 (4) ◽  
pp. 571-581 ◽  
Author(s):  
John Milton Mishler ◽  
Daniel C. Hadlock ◽  
Ignacio E. Fortuny ◽  
Robert W. Nicora ◽  
Jeffrey J. McCullough
Keyword(s):  
Hydroxyethyl Starch ◽  
Continuous Flow ◽  
Malignant Disease ◽  
Supportive Therapy ◽  
Control Group ◽  
Input Line ◽  
Healthy Donors ◽  
And Control ◽  
Continuous Flow Centrifuge ◽  
Differential Counts

Abstract A total of 67 leukaphereses were performed with the continuous-flow centrifuge (CFC) on 27 healthy donors for the purpose of obtaining increased yields of granulocytes for infusion into septic patients with acute leukemia accompanied by severe granulocytopenia. The addition of hydroxyethyl starch (HES) to the input line of the CFC significantly (p < 0.005) increased the total number of leukocytes and/or granulocytes collected per donation. A mean yield of 9.72 x 109 and 4.65 x 109 total granulocytes were collected by the HES-treated and control-group donors, respectively. The efficiency of cell collection as evidenced by the total number of leukocytes and/or granulocytes harvested per liter of processed blood was also significantly (p < 0.005) improved by the addition of HES to the continuous-flow centrifuge. Significant alterations in hematologic parameters were not experienced by HES-treated donors undergoing initial and multiple procedures. Pre- and postdonation leukocyte and platelet counts, hemoglobin, prothrombin time, partial thromboplastin time, and leukocyte differential counts were no different whether or not HES was employed for granulocyte collection. The results of the present study demonstrate that increased yields of granulocytes can be harvested by the addition of HES to the continuous-flow centrifuge and made available for supportive therapy to patients experiencing granulocytopenia induced by malignant disease or its treatment.

Systematic Use Of Heparin In The Continuous Flow Centrifuge (CFC) For Blood Cell Separation

1981 ◽  
Author(s):  
M R Morales ◽  
J Pizzuto ◽  
Ma Reyna ◽  
G Castro
Keyword(s):  
Blood Cell ◽  
Continuous Flow ◽  
Cell Separation ◽  
Reliable Method ◽  
Dosage Level ◽  
Thrombin Time ◽  
Coagulation Disorders ◽  
Time Dilution ◽  
Continuous Flow Centrifuge ◽  
Simultaneous Assessment

To date the use of heparin in the CFC has not been adequately controlled, thus exposing donors and patients to coagulation disorders. For this reason, we decided to evaluate the use of heparin by continuous infusion in dosages that would be modified by a simultaneous assessment of its anticoagulant effect, as shown by the thrombin time dilution test (TTDT).The study was performed during 46 leukopher- esis and 27 plasmapheresis. It was ascertained that heparin is an efficient anticoagulant in the CFC, using the TTDT as an effective and reliable method for its control. The initial dose in leukopheresis is one unit per milliliter of blood during the first hour, then half the dose during the next hour, and then a quarter of the dose until the procedure is completed. A TTDT performed every hour will indicate whether the amount of heparin used should be modified. For plasmapheresis, it is neccesary to establish a specific dose in each instance, using the TTDT as described. In most of the subjects, the anticoagulant level was exactly right. There was no case of bleeding or extracorporeal coagulation of the blood.On the basis of these findings, we recommend the use of heparin in the CFC, applying the results of the TTDT as a guide for its dosage level.

The Effect of a Single or Double Dose of Dexamethasone on Granulocyte Collection with the Continuous Flow Centrifuge

Vox Sanguinis ◽  
1975 ◽  
Vol 28 (3) ◽  
pp. 243-248 ◽  
Author(s):  
D. J. Higby ◽  
John Milton Mishler ◽  
W. Rhomberg ◽  
R. W. Nicora ◽  
J. F. Holland
Keyword(s):  
Continuous Flow ◽  
Double Dose ◽  
Continuous Flow Centrifuge

Design and construction of a ventilated cabinet for a continuous flow centrifuge

1974 ◽  
Vol 16 (12) ◽  
pp. 1681-1687 ◽  
Author(s):  
C. G. T. Evans ◽  
R. Harris-Smith ◽  
J. E. D. Stratton ◽  
J. Melling
Keyword(s):  
Continuous Flow ◽  
Continuous Flow Centrifuge ◽  
Design And Construction

Use of heparin for cytapheresis and plasmapheresis in a continuous flow centrifuge

Transfusion ◽  
1982 ◽  
Vol 22 (5) ◽  
pp. 384-387 ◽  
Author(s):  
M Morales ◽  
J Pizzuto ◽  
M Reyna ◽  
R Ambriz ◽  
A Aviles ◽  
...  
Keyword(s):  
Continuous Flow ◽  
Continuous Flow Centrifuge

Application of a Novel Continuous‐Flow Cell Separation Method for Separation of Cultured Human Mast Cells

2005 ◽  
Vol 28 (12-13) ◽  
pp. 2071-2083 ◽  
Author(s):  
Hiroyuki Shiono ◽  
Tadashi Okada ◽  
Yoichiro Ito
Keyword(s):  
Mast Cells ◽  
Continuous Flow ◽  
Cell Separation ◽  
Flow Cell ◽  
Separation Method ◽  
Human Mast Cells

Fractional Cleanout in a Continuous-Flow Centrifuge

1970 ◽  
Vol 5 (2) ◽  
pp. 137-143 ◽  
Author(s):  
W. K. Sartory
Keyword(s):  
Continuous Flow ◽  
Continuous Flow Centrifuge
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