MEET THE GUEST EDITOR [Hot Topic: Modern Omics-Based Platform for High Throughput Screening for Novel Drug Targets (Part 2) (Guest Editor: Canhua Huang)]

2012 ◽  
Vol 15 (4) ◽  
pp. 338-338
Author(s):  
Canhua Huang
Keyword(s):  
High Throughput ◽  
Guest Editor ◽  
High Throughput Screening ◽  
Drug Targets ◽  
Novel Drug ◽  
Novel Drug Targets

Abstract 1925: High-throughput drug library screening identifies potent drugs and novel drug targets for high-risk acute leukemia in children

2018 ◽  
Author(s):  
Priscilla Wander ◽  
Sandra S. Pinhanços ◽  
Bianca Koopmans ◽  
M. Emmy M. Dolman ◽  
Pauline Schneider ◽  
...  
Keyword(s):  
High Risk ◽  
Acute Leukemia ◽  
High Throughput ◽  
Drug Targets ◽  
Library Screening ◽  
Novel Drug ◽  
Novel Drug Targets ◽  
Leukemia In Children

Designing, optimizing, and implementing high-throughput siRNA genomic screening with glioma cells for the discovery of survival genes and novel drug targets

2010 ◽  
Vol 185 (2) ◽  
pp. 204-212 ◽  
Author(s):  
Nikhil G. Thaker ◽  
Peter R. McDonald ◽  
Fang Zhang ◽  
Carolyn A. Kitchens ◽  
Tong Ying Shun ◽  
...  
Keyword(s):  
High Throughput ◽  
Drug Targets ◽  
Glioma Cells ◽  
Genomic Screening ◽  
Novel Drug ◽  
Novel Drug Targets

scholarly journals A novel FRET-based screen in high-throughput format to identify inhibitors of malarial and human glucose transporters

2016 ◽  
pp. AAC.00218-16 ◽  
Author(s):  
Thomas E. Kraft ◽  
Monique R. Heitmeier ◽  
Marina Putanko ◽  
Rachel L. Edwards ◽  
Ma. Xenia G. Ilagan ◽  
...  
Keyword(s):  
High Throughput ◽  
High Throughput Screening ◽  
Drug Targets ◽  
Glucose Transporter ◽  
Glucose Sensor ◽  
Resonance Energy Transfer ◽  
Resonance Energy ◽  
Pharmacological Intervention ◽  
A Cell ◽  
Novel Drug

The glucose transporter PfHT is essential to the survival of the malaria parasitePlasmodium falciparumand has been shown to be a druggable target with high potential for pharmacological intervention. Identification of compounds against novel drug targets is crucial to combating resistance against current therapeutics. Here, we describe the development of a cell-based assay system readily adaptable to high-throughput screening that directly measures compound effects on PfHT-mediated glucose transport. Intracellular glucose concentrations are detected using a genetically encoded fluorescence resonance energy transfer (FRET)-based glucose-sensor. This allows assessment of the ability of small molecules to inhibit glucose uptake with high accuracy (Z'-factor of >0.8), thereby eliminating the need for radiolabeled substrates. Furthermore, we have adapted this assay to counter screen PfHT hits against the human orthologues GLUT1, 2, 3 and 4. We report the identification of several hits after screening the Medicines for Malaria Venture (MMV) Malaria Box, a library of 400 compounds known to inhibit erythrocytic development ofP. falciparum. Hit compounds were characterized by determining the half-maximal inhibitory concentration (IC50) for the uptake of radiolabeled glucose into isolatedP. falciparumparasites. One of our hits, compound MMV009085, shows high potency and ortholog selectivity, thereby successfully validating our assay for anti-malarial screening.

scholarly journals High-Throughput Analysis of Genome-Wide Receptor Tyrosine Kinase Expression in Human Cancers Identifies Potential Novel Drug Targets

2004 ◽  
Vol 10 (4) ◽  
pp. 1241-1249 ◽  
Author(s):  
Carsten Müller-Tidow ◽  
Joachim Schwäble ◽  
Björn Steffen ◽  
Nicola Tidow ◽  
Burkhardt Brandt ◽  
...  
Keyword(s):  
Tyrosine Kinase ◽  
High Throughput ◽  
Receptor Tyrosine Kinase ◽  
Drug Targets ◽  
High Throughput Analysis ◽  
Throughput Analysis ◽  
Genome Wide ◽  
Kinase Expression ◽  
Novel Drug ◽  
Novel Drug Targets

Novel drug targets in 2019

2020 ◽  
Vol 19 (5) ◽  
pp. 300-300 ◽  
Author(s):  
Sorin Avram ◽  
Liliana Halip ◽  
Ramona Curpan ◽  
Tudor I. Oprea
Keyword(s):  
Drug Targets ◽  
Novel Drug ◽  
Novel Drug Targets
Sign in / Sign up

Export Citation Format

Share Document