”BIK/NBK Gene Expression as a Possible Marker of Circulating Breast
Cancer Cells in Blood“

The detection of circulating breast cancer cells in blood could be of special interest as an indicator of diagnosis and prognosis, and for the selection of treatment. In a previous report, our research group determined gene expression profiles in samples of breast cancer tissue, identifying over-expression of the BIK/NBK mRNA gene in 90% of the analyzed samples. In this paper, we analyze the BIK/NBK gene expression as a possible biomarker of circulating breast cancer cells in blood. We demonstrate that the BIK/NBK gene expression is not a significant biomarker in the detection of circulating breast cancer cells in the blood of women with breast cancer. Several studies have evaluated the regulation of apoptosis by estrogens in breast cancer cells, demonstrating the importance of BIK/NBK protein, in estrogen-regulated breast cancer cell apoptosis, which suggests that the regulation of its expression may be an important therapeutic target or strategy in the management of cancer, and, although we did not find statistically significant differences among the patient groups to demonstrate that BIK/NBK gene expression is a biomarker of circulating breast cancer cells in blood, we consider it necessary to continue the study of this gene in breast cancer tissue and its role in the development and progression of breast cancer, its prognostic value, and its potential use as therapeutic target.

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Comparison of the global gene expression profiles produced by methylparaben,n-butylparaben and 17β-oestradiol in MCF7 human breast cancer cells

Journal of Applied Toxicology ◽

10.1002/jat.1200 ◽

2006 ◽

Vol 27 (1) ◽

pp. 67-77 ◽

Cited By ~ 34

Author(s):

D. Pugazhendhi ◽

A. J. Sadler ◽

P. D. Darbre

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Cancer Cells ◽

Human Breast Cancer ◽

Breast Cancer Cells ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Global Gene Expression ◽

Human Breast Cancer Cells ◽

Human Breast

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Gene Expression Profiles of Different Breast Cancer Cells Compared with their Responsiveness to Fermented Mistletoe (Viscum album L.) Extracts Iscador from Oak (Quercus), Pine (Pinus), White Fir (Abies) and Apple Tree (Malus) in vitro

Arzneimittelforschung ◽

10.1055/s-0031-1296816 ◽

2011 ◽

Vol 56 (06) ◽

pp. 483-496 ◽

Cited By ~ 1

Author(s):

Jenny Eggenschwiler ◽

Andrea Patrignani ◽

Ulrich Wagner ◽

Hubert Rehrauer ◽

Ralph Schlapbach ◽

...

Keyword(s):

Breast Cancer ◽

Gene Expression ◽

Cancer Cells ◽

Breast Cancer Cells ◽

Apple Tree ◽

Expression Profiles ◽

Gene Expression Profiles ◽

Viscum Album ◽

White Fir

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Identification of AHSA1 as a Potential Therapeutic Target for Breast Cancer: Bioinformatics Analysis and In Vitro Studies

Current Cancer Drug Targets ◽

10.2174/1568009622666220114151058 ◽

2022 ◽

Vol 22 ◽

Author(s):

Wei Shi ◽

Lu Qi ◽

Xiong-Bin You ◽

Yu-Chi Chen ◽

Yu-Lian Xu ◽

...

Keyword(s):

Breast Cancer ◽

Heat Shock ◽

Cancer Cells ◽

Therapeutic Target ◽

Breast Cancer Cells ◽

Bioinformatics Analysis ◽

Expression Profiles ◽

Migration And Invasion ◽

Network Pharmacology ◽

Cancer Tissue

Background: Shenling Baizhu Powder (SBP), a famous Traditional Chinese Medicine (TCM) formulation, has been widely used in the adjuvant treatment of cancers, including breast cancer. This study aims to identify potential new targets for breast cancer treatment based on the network pharmacology of SBP. Methods: By analyzing the relationship between herbs and target proteins, potential targets of multiple herbs in SBP were identified by network pharmacology analysis. Besides, by comparing the data of breast cancer tissue with normal tissue, upregulated genes in two breast cancer expression profiles were found. Thereafter, the expression level and prognosis of activator of heat shock protein 90 (HSP90) ATPase activity 1 (AHSA1) were further analyzed in breast cancer by bioinformatics analysis, and the network module of AHSA1 binding protein was constructed. Furthermore, the effect of knocking down AHSA1 on the proliferation, migration, and invasion of breast cancer cells was verified by MTT, clone formation assay, and transwell assay. Results: Vascular endothelial growth factor A (VEGFA), intercellular adhesion molecule 1 (ICAM1), chemokine (C-X-C motif) ligand 8 (CXCL8), AHSA1, and serpin family E member 1 (SERPINE1) were associated with multiple herbs in SBP. AHSA1 was remarkably upregulated in breast cancer tissues and positively correlated with poor overall survival and disease metastasis-free survival. Furthermore, knockdown of AHSA1 significantly inhibited the migration and invasion in MCF-7 and MDA-MB-231 breast cancer cells but had no obvious effect on proliferation. In addition, among the proteins that bind to AHSAl, the network composed of proteasome, chaperonin, and heat shock proteins is closely connected, and these proteins are associated with poor prognosis in a variety of cancers. Conclusion: AHSA1 is positively correlated with breast cancer progression and might act as a novel therapeutic target for breast cancer.

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