Chemical Composition and in-vitro antimicrobial screening of leaf essential oil of Plectranthus gerardianus Benth., and isolation of the major constituent of oil

2020 ◽  
Vol 10 ◽  
Author(s):  
Navadha Bhatt ◽  
Navabha Joshi ◽  
Kapil Ghai ◽  
Om Prakash
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Major Constituent ◽  
In Vitro Antimicrobial Activity ◽  
Gram Positive Bacteria ◽  
Medicinal Value ◽  
Leaf Essential Oil ◽  
Plant Families ◽  
Total Oil

Background: The Lamiaceae (Labiatae) is one of the most diverse and widespread plant families’ in terms of ethno medicine and its medicinal value is based on the volatile oils concentration. This family is important for flavour, fragrance and medicinal properties. Manyplants belonging to this family have indigenous value. Method: The essential oil of Plectranthus gerardianusBenth. (Lamiaceae), was analysed by GC and GC-MS analysis, while the major component was isolated and conformed by NMR spectroscopy. Result: The oil was found to be rich in oxygenated monoterpenes, which contribute around 62% of the total oil. The major components identified were fenchone (22.90%) and carvenone oxide (16.75%), besides other mono and sesquiterpenoids. The in-vitro antimicrobial activity of essential oil was tested against three gram negative bacteria viz. Pasteurellamultocida, Escherichia coli, and Salmonella enterica, two gram positive bacteria viz. Staphylococcus aureus and Bacillus subtilis and two fungi viz. Candida albicans and Aspergillusflavus. The antimicrobial activity of the oil was also compared to the antimicrobial activity of leaf essential oil of another Himalayan plant viz. Nepetacoerulescens. Conclusion: The oil showed in-vitro antimicrobial activity against all the microbial strains and can lessen the ever-growing demand of potentially hazardous antibiotics for treatment.

In vitro antimicrobial activity of the leaf essential oil of Spiraea alpina Pall.

2009 ◽  
Vol 26 (1) ◽  
pp. 9-14 ◽  
Author(s):  
Yun Teng ◽  
Qian Yang ◽  
Zhiyi Yu ◽  
Guoping Zhou ◽  
Qiu Sun ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
In Vitro Antimicrobial Activity ◽  
Leaf Essential Oil

scholarly journals Composition and in vitro antimicrobial activity of the essential oil of Dorema ammoniacum D. Don. fruit from Iran

2011 ◽  
Vol 76 (6) ◽  
pp. 857-863 ◽  
Author(s):  
Morteza Yousefzadi ◽  
Hossein Mirjalili ◽  
Naba Alnajar ◽  
Amineh Zeinali ◽  
Mitra Parsa
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Staphylococcus Epidermidis ◽  
Diffusion Method ◽  
Gram Negative Bacteria ◽  
In Vitro Antimicrobial Activity ◽  
Main Components ◽  
Total Oil ◽  
Antimicrobial Assay

The genus Dorema (Apiaceae) is represented in the flora of Iran with seven species of which two, D. ammoniacum D. Don. and D. aucheri Boiss. are endemic. Ripe fruits of D. ammoniacum collected just in the deciduous time were subjected to hydrodistillation to yield the essential oil, which was subsequently analyzed by GC and GC-MS. Twenty-nine compounds were identified and quantified, representing 95.1 % of the total oil. (Z)-Ocimenone (22.3 %) and (E)-ocimenone (18.1 %) were the main components of the oil. In vitro antimicrobial activity of the oil was evaluated against seven Gram-positive and Gram-negative bacteria (Bacillus subtilis, Enterococcus faecalis, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) and three fungi (Candida albicans, Saccharomyces cerevisiae and Aspergillus niger). The results of the antimicrobial assay of the oil by the disc diffusion method and the MIC values indicated that the oil exhibited moderate to high antimicrobial activity, especially against B. subtilis and S. epidermidis with MIC value of 3.75 mg ml-1.

In vitro antimicrobial activity of the essential oil from Vanillosmopsis arborea Barker (Asteraceae) and its major constituent, α-bisabolol

2018 ◽  
Vol 125 ◽  
pp. 144-149 ◽  
Author(s):  
Fabíola Fernandes Galvão Rodrigues ◽  
Aracélio Viana Colares ◽  
Carla de Fatima Alves Nonato ◽  
Fabío Fernandes Galvão-Rodrigues ◽  
Magaly Lima Mota ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Major Constituent ◽  
In Vitro Antimicrobial Activity

scholarly journals Chemical Composition and Antimicrobial Activity of Anthriscus nemorosa Root Essential Oil

2011 ◽  
Vol 6 (2) ◽  
pp. 1934578X1100600
Author(s):  
Milica Pavlović ◽  
Silvana Petrović ◽  
Marina Milenković ◽  
Maria Couladis ◽  
Olga Tzakou ◽  
...  
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
Gram Positive Bacteria ◽  
Microdilution Method ◽  
Broth Microdilution Method ◽  
Bacillus Subtilis Atcc ◽  
Germacrene D ◽  
Main Components ◽  
Total Oil ◽  
Root Essential Oil

The essential oil obtained by hydrodistillation from the roots of Anthriscus nemorosa (Bieb.) Sprengel (Umbelliferae) was analyzed by GC and GC-MS. Among sixty-two compounds identified (representing 89.0% of the total oil), the main components were: n-nonane (12.1%), n-hexadecanol (6.9%), δ-cadinene (6.4%), β-pinene (6.0%) and germacrene D (5.4%). Furthermore, the antimicrobial activity of the oil was evaluated against the Gram-positive bacteria Staphylococcus epidermidis (ATCC 12228) and Bacillus subtilis (ATCC 6633), the Gramnegative bacterium Escherichia coli (ATCC 25922), and a yeast Candida albicans (ATCC 10259 and ATCC 24433) using the broth microdilution method.

scholarly journals Chemical Composition, Antimicrobial, Antiradical and Anticholinesterase activity of the Essential Oil of Pulicaria stephanocarpa from Soqotra

2012 ◽  
Vol 7 (1) ◽  
pp. 1934578X1200700
Author(s):  
Nasser A. Awadh Ali ◽  
Rebecca A. Crouch ◽  
Mohamed A. Al-Fatimi ◽  
Norbert Arnold ◽  
Axel Teichert ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Essential Oil ◽  
Radical Scavenging ◽  
Gram Positive Bacteria ◽  
Ache Inhibitory Activity ◽  
Leaf Essential Oil ◽  
Radical Scavenging Ability ◽  
Main Components ◽  
Total Oil ◽  
Ellman’S Method

The chemical composition of the hydrodistilled leaf essential oil from Pulicaria stephanocarpa Balf Fil was determined by GC-MS analysis, and its antimicrobial, antioxidant and anticholinesterase (AChE) activities were evaluated. Eighty-three compounds were identified representing 97.2% of the total oil. ( E)-Caryophyllene 13.4%, ( E)-nerolidol 8.5%, caryophyllene oxide 8.5%, α-cadinol 8.2% spathulenol 6.8% and τ-cadinol 4.7%, were the main components. Antimicrobial activity of the oil, evaluated using the disc diffusion and broth dilution methods, demonstrated the highest susceptibility on Gram-positive bacteria and Candida albicans. The free radical scavenging ability of the oil was assessed by the DPPH assay to show antiradical activity with IC50 of 330 μg/mL. Moreover, the oil revealed an AChE inhibitory activity of 47% at a concentration of 200 μg/mL using Ellman's method.

Chemical Composition And In-Vitro Antimicrobial Activity Of The Essential Oils Of Three Greek Achillea Species

2002 ◽  
Vol 57 (3-4) ◽  
pp. 287-290 ◽  
Author(s):  
Prokopios Magiatis ◽  
Alexios-Leandros Skaltsounis ◽  
Ioanna Chinou ◽  
Serkos A. Haroutounian
Keyword(s):  
Antimicrobial Activity ◽  
Chemical Composition ◽  
Essential Oil ◽  
Essential Oils ◽  
Pathogenic Fungi ◽  
The Other ◽  
Gram Negative ◽  
In Vitro Antimicrobial Activity ◽  
Ms Analysis

The chemical composition of the essential oils of Achillea holosericea, Achillea taygetea, Achillea fraasii was determined by GC/MS analysis. Among the ninety-five assayed constituents, camphor, borneol and 1,8-cineol were found to be the major components. The in-vitro antimicrobial activity of these essential oils was evaluated against six bacteria indicating that the first is totally inactive, while the other two possess moderate to strong activities mainly against the Gram negative strains. The essential oil of A. fraasii was also active against the tested pathogenic fungi

The constituents of essential oil and in vitro antimicrobial activity of Micromeria cilicica from Turkey

2004 ◽  
Vol 94 (1) ◽  
pp. 43-48 ◽  
Author(s):  
Mehmet Emin Duru ◽  
Mehmet Öztürk ◽  
Aysel Uğur ◽  
Özgür Ceylan
Keyword(s):  
Antimicrobial Activity ◽  
Essential Oil ◽  
In Vitro Antimicrobial Activity
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