Antinociceptive Investigations of Niranthin in Complete Freund’s Adjuvant-induced Chronic Pain in Mice

2022 ◽  
Vol 16 ◽  
Author(s):  
Atul R. Chopade ◽  
Vijay R. Salunkhe ◽  
Pramod A. Patil ◽  
Madhav R. Burade ◽  
Prakash M. Somade ◽  
...  
Keyword(s):  
Thermal Sensitivity ◽  
Complete Freund’S Adjuvant ◽  
Paw Edema ◽  
Multiple Dosing ◽  
Pain Model ◽  
Mechanical Hypersensitivity ◽  
Highly Active ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

Abstract: The main objectives of the present work are to determine the clinical effect of niranthin on visceral or somatic inflammatory pain. The study was performed to determine the effects of niranthin on visceral or somatic inflammatory hypersensitivity of adult Swiss albino mice by using complete Freund’s adjuvant (CFA) induced pain model. The effect of CFA injection was determined after 24 hours of injection by using an aesthesiometer such as Von Frey filaments to evaluate tactile acetone-evoked cooling and thermal sensitivity. We used a digital Plethysmometer to measure paw edema. Single dose of niranthin intraperitoneal injection (5 & 10 mg/kg) was injected into mice having CFA-induced mechanical hypersensitivity and after 30 minutes of administration, reduced mechanical hypersensitivity was observed. In addition, niranthin also reduced acetone-evoked hypersensitivity within 4 hours. Compared to DMSO, niranthin was most highly active to reduce CFA-induced paw edema. To reduce mechanical hypersensitivity, multiple doses of niranthin (bis in die (b.i.d.)) from 1st - 5th day and b.i.d. day 9th and 10th) were given and remarkable results were observed such as did not cause tolerance in multiple dosing and significantly reduced in CFA induced hypersensitivity. This work reported niranthin having antinociceptive activity and indicated that niranthin is conventionally active in the management of persistent pain.

Anti-inflammatory Activity of Taiwan Folk Medicine "Ham-Hong-Chho" in Rats

1995 ◽  
Vol 23 (03n04) ◽  
pp. 273-278 ◽  
Author(s):  
Hwa-Woei Chih ◽  
Chun-Ching Lin ◽  
Kung-Sheng Tang
Keyword(s):  
Folk Medicine ◽  
Chronic Arthritis ◽  
Complete Freund’S Adjuvant ◽  
Aqueous Extracts ◽  
Paw Edema ◽  
Bidens Pilosa ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Anti Inflammatory

"Ham-Hong-Chho" is a folk medicine in Taiwan, derived from the entire plants of Bidens pilosa L. var. minor (Blume) Sherff (Compositae), B. pilosa L. and B. chilensis DC. The anti-inflammatory effect of aqueous extracts of the three plants against paw edema induced by carrageenan and chronic arthritis induced by complete Freund's adjuvant were determined in rats. The results indicated that paw edema induced by carrageenan was significantly decreased by treatment with aqueous extracts (150 or 300 mg/kg) of all three plants ( p < 0.05) and that the effect of Bidens pilosa var. minor was the most potent. However, only extracts (500 mg/kg) of B. pilosa L. var. minor and B.pilosa L. significantly decreased the paw edema induced by complete Freund's adjuvant ( p < 0.05).

scholarly journals Electroacupuncture Confers Antinociceptive Effects via Inhibition of Glutamate Transporter Downregulation in Complete Freund's Adjuvant-Injected Rats

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Ha-Neui Kim ◽  
Yu-Ri Kim ◽  
Ji-Yeon Jang ◽  
Hwa-Kyoung Shin ◽  
Byung-Tae Choi
Keyword(s):  
Thermal Sensitivity ◽  
Antinociceptive Effect ◽  
Glutamate Transporter ◽  
Complete Freund’S Adjuvant ◽  
Double Labeling ◽  
Mrna Content ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Glutamate Transporter 1

When we evaluated changes of glial fibrillary acidic protein (GFAP) and two glutamate transporter (GTs) by immunohistochemistry, expression of GFAP showed a significant increase in complete Freund's adjuvant (CFA)-injected rats; however, this expression was strongly inhibited by electroacupuncture (EA) stimulation. Robust downregulation of glutamate-aspartate transporter (GLAST) and glutamate transporter-1 (GLT-1) was observed in CFA-injected rats; however, EA stimulation resulted in recovery of this expression. Double-labeling staining showed co-localization of a large proportion of GLAST or GLT-1 with GFAP. Using Western blot, we confirmed protein expression of two GTs, but no differences in the mRNA content of these GTs were observed. Because EA treatment resulted in strong inhibition of CFA-induced proteasome activities, we examined the question of whether thermal sensitivities and GTs expression could be regulated by proteasome inhibitor MG132. CFA-injected rats co-treated with EA and MG132 showed a significantly longer thermal sensitivity, compared with CFA-injected rats with or without MG132. Both EA and MG132 blocked CFA-induced GLAST and GLT-1 downregulation within the spinal cord. These results provide evidence for involvement of GLAST and GLT-1 in response to activation of spinal astrocytes in an EA antinociceptive effect. Antinociceptive effect of EA may be induced via proteasome-mediated regulation of spinal GTs.

scholarly journals The potency of bay leaves extract (Syzygiumpolyanthum) as anti-inflammation in Rattus norvegicus induced with Complete Freund’s Adjuvant

2019 ◽  
Vol 8 (2) ◽  
pp. 64-69
Author(s):  
Dwi Candra Buana ◽  
I Ketut Sudiana ◽  
Jusak Nugraha
Keyword(s):  
Rattus Norvegicus ◽  
Positive Control ◽  
Complete Freund’S Adjuvant ◽  
Male Rat ◽  
Paw Edema ◽  
Herbal Plants ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Anti Inflammation

The using of herbal plants in Indonesia as medicine based on traditional knowledge. One of the herbal plants that believed has medication effect is bay leaves (Syzygiumpolyanthum). Bay leaves contain a flavonoid that can prevent inflammation. The study aims to prove the potency of bay leaves extract to prevent the inflammation, which is indicated by decreasing the paw edema thickness in rat induced with Complete Freund's Adjuvant (CFA). This study used 25 animal models of male rat (Rattus norvegicus) strain Wistar that divided into five groups, one group for a normal group (no treatment), one group for positive control which was injected with CFA sub plantar and given treatment with Na CMC and three test groups which is got three different doses of bay leaves extract peroral and the doses were 100 mg/kg BW, 300 mg/kg BW, 400 mg/kg BW, and injected with CFA into the sub plantar. Bay leaves extract was given one hour per oral before injected with CFA. The measurement of paw edema was done at 24 hours after CFA injection. The result of this study showed that bay leaves extract gave the anti-inflammation effect to the rats which were injected with CFA. It is concluded that bay leaves extract containing flavonoids plays a role in inhibit cytokines proinflammatory synthesis.

scholarly journals Evaluation of anti-inflammatory effect of beetroot extract in animal models

2017 ◽  
Vol 6 (12) ◽  
pp. 2853 ◽  
Author(s):  
Anjan Adhikari ◽  
Aioshi Saha ◽  
Rania Indu ◽  
Tapas Kumar Sur ◽  
Anup Kumar Das
Keyword(s):  
Complete Freund’S Adjuvant ◽  
Paw Edema ◽  
Standard Drug ◽  
Rat Paw Edema ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Inflammatory Effect

Background: Inflammation is complex biological response of body tissues to foreign particles or pathogens. However, adverse effects of anti-inflammatory drugs, necessitate the search for a novel compound from herbal origin. Beta vulgaris rubra or red beetroot, a vegetable plant, is known to possess various beneficial effects. Present study aimed to evaluate the anti inflammatory effect of beet roots in animal models.Methods: Carrageenan-induced and Complete Freund’s adjuvant-induced rat paw edema model were used to assess the acute and chronic anti- inflammatory potential of the Beetroot as compared to standard drug, Diclofenac Sodium.Results: Oral administration of Beetroot extract showed carrageenan-induced rat paw edema inhibition by 26.9%, 34.6% and 50% for beetroot 100mg/Kg, 200mg/Kg and 400mg/Kg, respectively, at the end of four hours. In vivo results noted that percentage inhibition of paw volume and ankle diameter of the Complete Freund’s adjuvant-induced rats were significantly higher for the groups consuming 100 and 200mg/Kg Beetroot extracts. Paw volume of the rats in these two groups reduced by 35% and 30% respectively as compared to the control.Conclusions: Thus, beetroot was found to possess anti-inflammatory property in animals. Further investigations are essential to validate the bioactive components responsible for this property.

scholarly journals Appraisal of Anti-Arthritic Potential of Quercus Leucotrichophora Methanolic Extract in Complete Freund’s Adjuvant Induced Arthritic Rats; a Mechanistic Study

2022 ◽  
Author(s):  
Ammara Saleem ◽  
Izza Hameed ◽  
Muhammad Furqan Akhtar ◽  
Md. Habibur Rahman ◽  
Ghulam Md Ash
Keyword(s):  
Research Work ◽  
Sinapic Acid ◽  
Complete Freund’S Adjuvant ◽  
Mechanistic Study ◽  
Paw Edema ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Anti Inflammatory ◽  
Quercus Leucotrichophora

Abstract This research work was conducted to validate the folkloric use and therapeutic potential of Quercus leucotrichophora (QL) leaf methanolic and aqueous extracts against inflammation and arthritis and to determine the chemical composition by HPLC. The in-vitro anti-oxidant and anti-inflammatory activities were carried out along with in-vivo assays such as carrageenan induced paw edema, xylene induced ear edema and Complete Freund’s Adjuvant induced arthritis in Wistar rats. The CFA (0.1 ml) was inoculated to the left hind paw at day 1 to induce arthritis and oral dosing with QLME at 150, 300 and 600 mg/kg was begun at 8th day till the 28th day in all groups while methotrexate was given as standard treatment. There was a noteworthy (p<0.05-0.0001) restoration in body weight, paw edema, arthritic index, altered blood parameters and oxidative stress biomarkers in treated rats as compared to diseased group. Moreover, QLME considerably (p<0.0001) downregulated TNF-α, IL-6, IL-1β, COX-2, and NF-κB, while significantly (p<0.0001) upregulated IL-10, I-κB, IL-4 in relation to diseased group. The QLME exhibited no mortality in acute toxicity study. It was concluded that QLME possessed substantial anti-inflammatory and anti-arthritic potential at all dosage levels, mainly at 600 mg/kg might be due to presence of quercetin, sinapic acid and ferulic acid.

scholarly journals Effect of Electroacupuncture on Activation of p38MAPK in Spinal Dorsal Horn in Rats with Complete Freund's Adjuvant-Induced Inflammatory Pain

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Yi Liang ◽  
Jian-Qiao Fang ◽  
Jun-Ying Du ◽  
Jun-Fan Fang
Keyword(s):  
P38 Mapk ◽  
Inflammatory Pain ◽  
Saline Group ◽  
Complete Freund’S Adjuvant ◽  
Paw Edema ◽  
Intraplantar Injection ◽  
Male Adult ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

Activation of mitogen-activated protein kinases (MAPKs), especially p38 MAPK, plays an important role in the development of central sensitization related to persistent inflammatory pain. Electroacupuncture (EA) is well known to relieve persistent inflammatory pain. However, little is known about relationship between EA and p38 MAPK. Inflammatory pain rat model was induced by intraplantar injection of complete Freund's adjuvant (CFA). Male adult SD rats were randomly divided into the saline group, CFA group, and CFA + EA group. EA (constant saquare wave, 2 Hz and 100 Hz alternating frequencies, intensities ranging from 1 to 2 mA) was applied to bilateral “Zusanli” (ST 36) and “Kunlun” acupoints (BL 60) for 30 min, once per day. The paw edema and paw withdrawal threshold (PWT) were measured at preinjection and days postinjection 1, 3, and 14. Spinal p-p38MAPK- immunoreactivty (p-p38MAPK-IR) cells were detected by immunohistochemistry at postinjection day 3 and 14. EA significantly inhibited paw edema at postinjection days 14 and increased PWT at postinjection days 3 and 14. Moreover, the increasing number of spinal p-p38MAPK-IR cells which was induced by CFA injection was suppressed by EA stimulation. These results indicate that anti-inflammatory and analgesic effect of EA might be associated with its inhibition of spinal p38 MAPK activation and thereby provide a potential mechanism for the treatment of inflammatory pain by EA.

scholarly journals Total Western Diet Alters Mechanical and Thermal Sensitivity and Prolongs Hypersensitivity Following Complete Freund's Adjuvant in Mice

2016 ◽  
Vol 17 (1) ◽  
pp. 119-125 ◽  
Author(s):  
Stacie K. Totsch ◽  
Megan E. Waite ◽  
Ashleigh Tomkovich ◽  
Tammie L. Quinn ◽  
Barbara A. Gower ◽  
...  
Keyword(s):  
Thermal Sensitivity ◽  
Western Diet ◽  
Complete Freund’S Adjuvant ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant
Sign in / Sign up

Export Citation Format

Share Document