scholarly journals The potency of bay leaves extract (Syzygiumpolyanthum) as anti-inflammation in Rattus norvegicus induced with Complete Freund’s Adjuvant

2019 ◽  
Vol 8 (2) ◽  
pp. 64-69
Author(s):  
Dwi Candra Buana ◽  
I Ketut Sudiana ◽  
Jusak Nugraha
Keyword(s):  
Rattus Norvegicus ◽  
Positive Control ◽  
Complete Freund’S Adjuvant ◽  
Male Rat ◽  
Paw Edema ◽  
Herbal Plants ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Anti Inflammation

The using of herbal plants in Indonesia as medicine based on traditional knowledge. One of the herbal plants that believed has medication effect is bay leaves (Syzygiumpolyanthum). Bay leaves contain a flavonoid that can prevent inflammation. The study aims to prove the potency of bay leaves extract to prevent the inflammation, which is indicated by decreasing the paw edema thickness in rat induced with Complete Freund's Adjuvant (CFA). This study used 25 animal models of male rat (Rattus norvegicus) strain Wistar that divided into five groups, one group for a normal group (no treatment), one group for positive control which was injected with CFA sub plantar and given treatment with Na CMC and three test groups which is got three different doses of bay leaves extract peroral and the doses were 100 mg/kg BW, 300 mg/kg BW, 400 mg/kg BW, and injected with CFA into the sub plantar. Bay leaves extract was given one hour per oral before injected with CFA. The measurement of paw edema was done at 24 hours after CFA injection. The result of this study showed that bay leaves extract gave the anti-inflammation effect to the rats which were injected with CFA. It is concluded that bay leaves extract containing flavonoids plays a role in inhibit cytokines proinflammatory synthesis.

Anti-inflammatory Activity of Taiwan Folk Medicine "Ham-Hong-Chho" in Rats

1995 ◽  
Vol 23 (03n04) ◽  
pp. 273-278 ◽  
Author(s):  
Hwa-Woei Chih ◽  
Chun-Ching Lin ◽  
Kung-Sheng Tang
Keyword(s):  
Folk Medicine ◽  
Chronic Arthritis ◽  
Complete Freund’S Adjuvant ◽  
Aqueous Extracts ◽  
Paw Edema ◽  
Bidens Pilosa ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Anti Inflammatory

"Ham-Hong-Chho" is a folk medicine in Taiwan, derived from the entire plants of Bidens pilosa L. var. minor (Blume) Sherff (Compositae), B. pilosa L. and B. chilensis DC. The anti-inflammatory effect of aqueous extracts of the three plants against paw edema induced by carrageenan and chronic arthritis induced by complete Freund's adjuvant were determined in rats. The results indicated that paw edema induced by carrageenan was significantly decreased by treatment with aqueous extracts (150 or 300 mg/kg) of all three plants ( p < 0.05) and that the effect of Bidens pilosa var. minor was the most potent. However, only extracts (500 mg/kg) of B. pilosa L. var. minor and B.pilosa L. significantly decreased the paw edema induced by complete Freund's adjuvant ( p < 0.05).

Antinociceptive Investigations of Niranthin in Complete Freund’s Adjuvant-induced Chronic Pain in Mice

2022 ◽  
Vol 16 ◽  
Author(s):  
Atul R. Chopade ◽  
Vijay R. Salunkhe ◽  
Pramod A. Patil ◽  
Madhav R. Burade ◽  
Prakash M. Somade ◽  
...  
Keyword(s):  
Thermal Sensitivity ◽  
Complete Freund’S Adjuvant ◽  
Paw Edema ◽  
Multiple Dosing ◽  
Pain Model ◽  
Mechanical Hypersensitivity ◽  
Highly Active ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

Abstract: The main objectives of the present work are to determine the clinical effect of niranthin on visceral or somatic inflammatory pain. The study was performed to determine the effects of niranthin on visceral or somatic inflammatory hypersensitivity of adult Swiss albino mice by using complete Freund’s adjuvant (CFA) induced pain model. The effect of CFA injection was determined after 24 hours of injection by using an aesthesiometer such as Von Frey filaments to evaluate tactile acetone-evoked cooling and thermal sensitivity. We used a digital Plethysmometer to measure paw edema. Single dose of niranthin intraperitoneal injection (5 & 10 mg/kg) was injected into mice having CFA-induced mechanical hypersensitivity and after 30 minutes of administration, reduced mechanical hypersensitivity was observed. In addition, niranthin also reduced acetone-evoked hypersensitivity within 4 hours. Compared to DMSO, niranthin was most highly active to reduce CFA-induced paw edema. To reduce mechanical hypersensitivity, multiple doses of niranthin (bis in die (b.i.d.)) from 1st - 5th day and b.i.d. day 9th and 10th) were given and remarkable results were observed such as did not cause tolerance in multiple dosing and significantly reduced in CFA induced hypersensitivity. This work reported niranthin having antinociceptive activity and indicated that niranthin is conventionally active in the management of persistent pain.

scholarly journals Evaluation of anti-inflammatory effect of beetroot extract in animal models

2017 ◽  
Vol 6 (12) ◽  
pp. 2853 ◽  
Author(s):  
Anjan Adhikari ◽  
Aioshi Saha ◽  
Rania Indu ◽  
Tapas Kumar Sur ◽  
Anup Kumar Das
Keyword(s):  
Complete Freund’S Adjuvant ◽  
Paw Edema ◽  
Standard Drug ◽  
Rat Paw Edema ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Anti Inflammatory ◽  
Rat Paw ◽  
Inflammatory Effect

Background: Inflammation is complex biological response of body tissues to foreign particles or pathogens. However, adverse effects of anti-inflammatory drugs, necessitate the search for a novel compound from herbal origin. Beta vulgaris rubra or red beetroot, a vegetable plant, is known to possess various beneficial effects. Present study aimed to evaluate the anti inflammatory effect of beet roots in animal models.Methods: Carrageenan-induced and Complete Freund’s adjuvant-induced rat paw edema model were used to assess the acute and chronic anti- inflammatory potential of the Beetroot as compared to standard drug, Diclofenac Sodium.Results: Oral administration of Beetroot extract showed carrageenan-induced rat paw edema inhibition by 26.9%, 34.6% and 50% for beetroot 100mg/Kg, 200mg/Kg and 400mg/Kg, respectively, at the end of four hours. In vivo results noted that percentage inhibition of paw volume and ankle diameter of the Complete Freund’s adjuvant-induced rats were significantly higher for the groups consuming 100 and 200mg/Kg Beetroot extracts. Paw volume of the rats in these two groups reduced by 35% and 30% respectively as compared to the control.Conclusions: Thus, beetroot was found to possess anti-inflammatory property in animals. Further investigations are essential to validate the bioactive components responsible for this property.

scholarly journals Appraisal of Anti-Arthritic Potential of Quercus Leucotrichophora Methanolic Extract in Complete Freund’s Adjuvant Induced Arthritic Rats; a Mechanistic Study

2022 ◽  
Author(s):  
Ammara Saleem ◽  
Izza Hameed ◽  
Muhammad Furqan Akhtar ◽  
Md. Habibur Rahman ◽  
Ghulam Md Ash
Keyword(s):  
Research Work ◽  
Sinapic Acid ◽  
Complete Freund’S Adjuvant ◽  
Mechanistic Study ◽  
Paw Edema ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant ◽  
Anti Inflammatory ◽  
Quercus Leucotrichophora

Abstract This research work was conducted to validate the folkloric use and therapeutic potential of Quercus leucotrichophora (QL) leaf methanolic and aqueous extracts against inflammation and arthritis and to determine the chemical composition by HPLC. The in-vitro anti-oxidant and anti-inflammatory activities were carried out along with in-vivo assays such as carrageenan induced paw edema, xylene induced ear edema and Complete Freund’s Adjuvant induced arthritis in Wistar rats. The CFA (0.1 ml) was inoculated to the left hind paw at day 1 to induce arthritis and oral dosing with QLME at 150, 300 and 600 mg/kg was begun at 8th day till the 28th day in all groups while methotrexate was given as standard treatment. There was a noteworthy (p<0.05-0.0001) restoration in body weight, paw edema, arthritic index, altered blood parameters and oxidative stress biomarkers in treated rats as compared to diseased group. Moreover, QLME considerably (p<0.0001) downregulated TNF-α, IL-6, IL-1β, COX-2, and NF-κB, while significantly (p<0.0001) upregulated IL-10, I-κB, IL-4 in relation to diseased group. The QLME exhibited no mortality in acute toxicity study. It was concluded that QLME possessed substantial anti-inflammatory and anti-arthritic potential at all dosage levels, mainly at 600 mg/kg might be due to presence of quercetin, sinapic acid and ferulic acid.

scholarly journals POTENCY AND EFFICACY TEST OF A VACCINE IN ADDITION WITH ADJUVANT AGAINST KOI HERPESVIRUS IN KOI (Cyprinus carpio)

2016 ◽  
Vol 11 (1) ◽  
pp. 41 ◽  
Author(s):  
Lili Sholichah ◽  
Munti Yuhana ◽  
Angela Mariana Lusiastuti ◽  
Tri Heru Prihadi
Keyword(s):  
Positive Control ◽  
Negative Control ◽  
Complete Freund’S Adjuvant ◽  
Post Inoculation ◽  
Inactivated Vaccine ◽  
Koi Herpesvirus ◽  
Test Fish ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

Koi Herpesvirus (KHV) is a malignant virus infecting the goldfish and koi in all stadia and cause mortality up to 95%. The purpose of this study was to determine the potency and efficacy of inactivated-vaccine in addition with adjuvant against KHV in koi fish. The viral propagation was done using a KF-1 cell line in 25 cm3 flask. The cultured virus was harvested on 12 days post inoculation, and then the harvested virus was inactivated with 0.1% formalin as inactivated-vaccine. Three hundred of test fish (10.38 ± 1.25 g) maintained in 126 L of plastic containers with aeration, and fed with pellets twice a day. After 14 days of adaptation, the fish were divided into five treatments (A= vaccine; B= vaccine + Complete Freund’s Adjuvant; C= vaccine + Incomplete Freund’s Adjuvant; K+= positive control, and K-= negative control) and each treatment has four replicates. Vaccine was given by injecting intramuscularly of 0.1 mL per fish. All fish were challenged by injecting intramuscularly of 0.1 mL of KHV virus with concentration of 104.58 TCID50/mL after 21 days post vaccination. The results showed that the B treatment had higher (P<0.05) values of hematocrit level, lysozyme activity, and titer of antibody compared with positive control. In addition, the survival of fish in B treatment also had the highest percentages and significantly different compared to other treatments (P<0.05). The conclusion of this research was the application of inactivated KHV vaccine in 0.1% formalin with the addition of Complete Freund’s Adjuvant through the injection dose 0.1 mL fish-1 in 104.58 TCID50/mL capable to enhance the immune responses and raised the optimal protection of KHV antibody in koi fish.

scholarly journals Effect of Electroacupuncture on Activation of p38MAPK in Spinal Dorsal Horn in Rats with Complete Freund's Adjuvant-Induced Inflammatory Pain

2012 ◽  
Vol 2012 ◽  
pp. 1-6 ◽  
Author(s):  
Yi Liang ◽  
Jian-Qiao Fang ◽  
Jun-Ying Du ◽  
Jun-Fan Fang
Keyword(s):  
P38 Mapk ◽  
Inflammatory Pain ◽  
Saline Group ◽  
Complete Freund’S Adjuvant ◽  
Paw Edema ◽  
Intraplantar Injection ◽  
Male Adult ◽  
Freund’S Adjuvant ◽  
Complete Freund's Adjuvant ◽  
Freund's Adjuvant

Activation of mitogen-activated protein kinases (MAPKs), especially p38 MAPK, plays an important role in the development of central sensitization related to persistent inflammatory pain. Electroacupuncture (EA) is well known to relieve persistent inflammatory pain. However, little is known about relationship between EA and p38 MAPK. Inflammatory pain rat model was induced by intraplantar injection of complete Freund's adjuvant (CFA). Male adult SD rats were randomly divided into the saline group, CFA group, and CFA + EA group. EA (constant saquare wave, 2 Hz and 100 Hz alternating frequencies, intensities ranging from 1 to 2 mA) was applied to bilateral “Zusanli” (ST 36) and “Kunlun” acupoints (BL 60) for 30 min, once per day. The paw edema and paw withdrawal threshold (PWT) were measured at preinjection and days postinjection 1, 3, and 14. Spinal p-p38MAPK- immunoreactivty (p-p38MAPK-IR) cells were detected by immunohistochemistry at postinjection day 3 and 14. EA significantly inhibited paw edema at postinjection days 14 and increased PWT at postinjection days 3 and 14. Moreover, the increasing number of spinal p-p38MAPK-IR cells which was induced by CFA injection was suppressed by EA stimulation. These results indicate that anti-inflammatory and analgesic effect of EA might be associated with its inhibition of spinal p38 MAPK activation and thereby provide a potential mechanism for the treatment of inflammatory pain by EA.

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