Isolation and characterization of native Azospirillium isolates from normal soils grown rice areas of Chhattisgarh

Sixty soil samples were collected from normal soils of Rice growing areas during year 2014-2015 from various blocks of Raipur district of Chattisgarh for isolation of Azospirillium. In this study 60 Azospirillium isolates were obtained and were characterized on the basis of Gram’s staining, starch hydrolysis, catalase test, urease test, gelatin liquefaction, growth performance in terms of intensity of blue colour produced in N-free malate broth, indole acetic acid (IAA) production and N-fixation capacity. Local Azospirillium isolate AZP-R03 was found most effective native Nitrogen fixing bacteria for Rice with a great potential for its use as nitrogen fixing bacterial fertilizer especially for Rice crop under agroclimatic conditions of Chattisgarh.

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Isolation and Characterization of Nitrogen-Fixing Bacteria and Producing IAA (Indole Acetic Acid) From Rice Rhizosphere from The Soppeng Regency

International Journal of Pharmaceutical Research ◽

10.31838/ijpr/2021.13.01.138 ◽

2020 ◽

Vol 13 (01) ◽

Keyword(s):

Acetic Acid ◽

Indole Acetic Acid ◽

Nitrogen Fixing ◽

Nitrogen Fixing Bacteria ◽

Rice Rhizosphere ◽

Isolation And Characterization

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Exploration, isolation and characterization of indigenous rhizobacteria from patchouli rhizosphere as PGPR candidates in producing IAA and solubilizing phosphate

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/951/1/012055 ◽

2022 ◽

Vol 951 (1) ◽

pp. 012055

Author(s):

H Halimursyadah ◽

Syamsuddin ◽

Nurhayati ◽

DN Rizva

Keyword(s):

Phosphate Solubilization ◽

Descriptive Analysis ◽

Soil Samples ◽

Gram Negative ◽

Iaa Production ◽

Isolation And Characterization ◽

Aceh Province ◽

Qualitative Descriptive ◽

Phosphate Solubilizing

Abstract Microorganisms that are active and aggressive colonizing the rhizosphere are known as rhizobacteria. They are able to act as biofertilizers, bioprotectants, biostimulants and bioremediation. This study aims to identify and characterize groups of rhizobacteria present in the patchouli rhizosphere that can produce IAA compounds and have the ability to solubilize phosphate in the soil. Soil samples were taken from the patchouli rhizosphere at Purwosari Village, Nagan Raya, Aceh Province, Indonesia. This study used quantitative and qualitative descriptive analysis through serial dilutions to obtain rhizobacterial strains. Parameters observed were macroscopic and microscopic characteristics, gram test, IAA production and phosphate solubilization. The study obtained 37 isolates of rhizobacteria from Purwosari (PS), comprising 25 isolates of gram positive and 12 isolates of gram negative. The rhizobacteria PS 5/1 produced the lowest IAA at 21.66 ppm, whereas isolate 5/6 C produced the highest IAA at 83.38 ppm. Twenty-five isolates of rhizobacteria could solubilize phosphate while the remaining 12 isolates did not have this ability. The rhizobacteria PS 7/1 resulted in the highest PSI at 2.55 and isolates PS 8/7 produced the lowest PSI at 1.33. The rhizobacteria isolates that can produce IAA and phosphate solubilizing have the potential to be used as PGPR candidates.

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PENGEMBANGAN TEKNOLOGI SELEKSI IN VITRO BERULANG (REPEAT CYCLING–IN VITRO SELECTION) TOLERAN STRES KEKERINGAN UNTUK PERBAIKAN MUTU GENETIK DAN PRODUKSI TANAMAN KEDELAI BERMIKORIZA

Jurnal Ilmiah Ilmu Terapan Universitas Jambi|JIITUJ| ◽

10.22437/jiituj.v1i1.3753 ◽

2017 ◽

Vol 1 (1) ◽

pp. 74-84

Author(s):

Ahmad Riduan ◽

Rainiyati Rainiyati ◽

Yulia Alia

Keyword(s):

Arbuscular Mycorrhizae ◽

In Vitro Selection ◽

Soil Samples ◽

Single Spore ◽

Isolation And Characterization ◽

Single Spore Culture ◽

Spore Culture ◽

Glomus Sp

Every plant rhizospheres in any ecosystem there are various living microorganisms including Arbuscular Mycorrhizae Fungi (AMF). An isolation and characterization is required to investigate the species or type of the AMF. This research was aimed at studying the isolation and characterization of AMF sporulation in soybean rhizospheres in Jambi Province. The results of evaluation on soil samples before trapping showed that there are spores from three genus of AMF twelve types Glomus , two types Acaulospora and one type of Enthrophospora. Following single spore culture in soybean rhizosphere, 5 spore types were obtained: Glomus sp-1, Glomus sp-4, Glomus sp-7, Glomus sp-8 Glomus sp-10.

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