scholarly journals Isolation and characterization of natural protease producers of Bacillus spp. from Soil samples

2018 ◽  
Author(s):  
Ylberina Baliu ◽  
Bahtir Hyseni ◽  
Shkëlqim Hyseni ◽  
Aida Rushiti ◽  
Flora Ferati ◽  
...  
Keyword(s):  
Soil Samples ◽  
Isolation And Characterization ◽  
Bacillus Spp

PENGEMBANGAN TEKNOLOGI SELEKSI IN VITRO BERULANG (REPEAT CYCLING–IN VITRO SELECTION) TOLERAN STRES KEKERINGAN UNTUK PERBAIKAN MUTU GENETIK DAN PRODUKSI TANAMAN KEDELAI BERMIKORIZA

2017 ◽  
Vol 1 (1) ◽  
pp. 74-84
Author(s):  
Ahmad Riduan ◽  
Rainiyati Rainiyati ◽  
Yulia Alia
Keyword(s):  
Arbuscular Mycorrhizae ◽  
In Vitro Selection ◽  
Soil Samples ◽  
Single Spore ◽  
Isolation And Characterization ◽  
Single Spore Culture ◽  
Spore Culture ◽  
Glomus Sp

Every plant rhizospheres in any ecosystem there are various living microorganisms including Arbuscular Mycorrhizae Fungi (AMF).  An isolation and characterization is required to investigate the species or type of the AMF. This research was aimed at studying the isolation and characterization of AMF sporulation in soybean rhizospheres in Jambi Province. The results of evaluation on soil samples before trapping showed that there are spores from three genus of AMF twelve types Glomus , two types Acaulospora and one type of Enthrophospora.  Following single spore culture in soybean rhizosphere, 5 spore types were obtained:  Glomus sp-1, Glomus sp-4, Glomus sp-7, Glomus sp-8 Glomus sp-10.

scholarly journals Isolation and Characterization of Xenobiotic Pesticide Degrading Bacterial Species in Flower Farms Around Lake Naivasha, Kenya

2018 ◽  
Vol 1 (1) ◽  
Keyword(s):  
Biochemical Characterization ◽  
Bacterial Species ◽  
Rhodococcus Erythropolis ◽  
Soil Samples ◽  
Lake Naivasha ◽  
Organophosphate Pesticide ◽  
Lower Growth ◽  
Isolation And Characterization ◽  
Cell Counts

Certain microorganisms especially bacteria and fungi are able to use xenobiotic organic compounds as their carbon and nitrogen source for metabolism. Flower farms around lake Naivasha basin uses several agrochemicals especially pesticides to control pests and improve flower production. The aim of this study was to isolate and characterize morphologically and biochemically the main bacterial species that are able to grow and tolerate the pesticide contaminated farm soils. Soil samples were collected from randomly selected five greenhouses from each five flower farms namely Crescent, Elsamere, Karuturi, Malewa and Sewage farms around Lake Naivasha basin. The collected samples were processed for bacterial isolation using the nutrient agar, mac’ Conkey agar, blood agar, Luria-Bertani and Minimum Salt Media nutrient media. The conventional methods of swabbing and streaking were used. Pure colonies of isolates organisms were identified and characterized using standard microbiological technique. Morphological, cultural and biochemical characterization of bacterial species isolated from the flower farm soil samples identified mainly Pseudomonas auriginosa, Escherichia coli, Rhodococcus erythropolis and Bacillus subtilis species. Bacterial growth in pesticide consortia was quantified by monitoring colony growth of the species in liquid culture over time. The viable cell counts were determined turbidimetrically at O.D696nm. All the isolated bacterial species were able to grow in flower farm soil contaminated with organochloride and organophosphate pesticide residues. B. subtilis recorded the highest growth at 1.77±0.07 O.D696nm in pesticide mixture consortia. There was lower growth in organochloride pesticide consortia as compared to organophosphate pesticide consortia.

scholarly journals Isolation and Characterization of Thermophilic Bacteria Indigenous to Al-Ahsa Desert

2020 ◽  
Vol 14 (3) ◽  
pp. 2157-2163
Author(s):  
Ahmed Anwar Al-Mulla ◽  
Ashraf Khalifa
Keyword(s):  
Standard Method ◽  
Thermophilic Bacteria ◽  
Soil Samples ◽  
Biotechnological Applications ◽  
Bacterial Isolates ◽  
Phenotypic Characteristics ◽  
Thermostable Enzymes ◽  
Biological Resources ◽  
Isolation And Characterization

Deciphering the biological resources across the Saudi niches is highly recommended for the prosperity. To this end, the aim of the current work was to isolate thermophilic bacteria from unexplored areas of Al-Ahsa region, and investigate their phenotypic characteristics. Three soil samples were collected from different desert sites of Al-Ahsa region. Thermophilic bacteria were isolated directly for soil samples into Thermus medium broth as a standard method. Single colonies of the actively growing bacterial isolates were preserved in 20% glycerol then kept at -80°C. The isolates were screened for production of thermostable enzymes using the commercially available kit API20E strip (bioMerieux, Marcy l’Etoile, France). Incubation were carried out at 50°C. It can be concluded that thermophilic bacteria in Al-Ahsa region harbor novel thermostable enzymes that might have biotechnological applications, in future.

scholarly journals ISOLATION AND CHARACTERIZATION OF DIESEL DEGRADING BACTERIA FROM PETROLEUM OIL CONTAMINATED SOIL

2021 ◽  
pp. e313
Author(s):  
Tanvir Ahmed ◽  
Sababa Alam ◽  
Tasnia Ahmed
Keyword(s):  
Contaminated Soil ◽  
Energy Production ◽  
Petroleum Products ◽  
Low Ph ◽  
Isolation And Characterization ◽  
Degrading Bacteria ◽  
Bacillus Spp ◽  
Higher Temperature ◽  
Petroleum Oil

Petroleum products are used for energy production and an essential part of our day-to-day lives especially in vehicles, ships, and industries. Accidental leakages occur easily and wastage petroleum is also discarded in the environment without any further processing causing environmental pollution. Diesel contributea big part topetroleum pollution. The current study was aimed to identify diesel degrading bacteria and determine some conditions to evaluate their best degradation capability. We identified Aeromonas spp., Bacillus spp., and Enterobacter spp. from diesel contaminated soil and found that Aeromonas spp. and Bacillus spp. grow best with 10% to 15% diesel whereas Enterobacter spp. can grow quite well with 20% diesel concentration at a higher temperature (40oC) than the previous two bacteria. Aeromonas spp. worked well at low pH (pH 4 to pH 6) whereas Bacillus spp. and Enterobacter spp. worked best at higher pH (pH 10).

scholarly journals Isolation and characterization of phosphate solubilizing bacteria from corn rhizosfer

2021 ◽  
Vol 911 (1) ◽  
pp. 012063
Author(s):  
Haswania ◽  
H Karim ◽  
A.A. Azis ◽  
N Iriany ◽  
O Jumadi
Keyword(s):  
Soil Samples ◽  
Phosphate Solubilizing Bacteria ◽  
Methyl Red ◽  
Biochemical Tests ◽  
Gram Staining ◽  
Isolation And Characterization ◽  
Sucrose Fermentation ◽  
Phosphate Solubilizing ◽  
Sample Dilution

Abstract The aim of this study was to isolate and characterize the Phosphate solubilizing bacteria from the rhizosphere of Zea mays L., Jeneponto Regency. This research was conducted in several stages; i.e, sampling, medium preparation, sample dilution, isolation, characterization in the form of gram staining, biochemical tests, and quantitative tests of phosphate solubility. Soil samples were diluted in 0.9% NaCl and soil containing microbes was isolated on the Picovskaya medium. Three isolates were obtained which could dissolve phosphate, namely J2KN1, J3KR2, and J3TG3 isolates. The isolates were generally round in shape with raised elevations, white, slimy, smooth, shiny surface, milky white, shape like coccus and bacillus, and gram-negative. Some of the isolates had positive motility, indole, voges, methyl red, glucose, and sucrose fermentation in the biochemical test. The quantitative tests of the ability to dissolve phosphate showed that J2KN1 isolate had the highest concentration of 51.1 μM, and the J3KR1 and J3TG3 isolates had a concentration of 45.2 μM and 37.6 μM, respectively.

scholarly journals Isolation and Characterization of Carbonoclastic Bacteria Diversity in Oil-contaminated Soil in Cape Coast Metropolis, Ghana

2020 ◽  
pp. 62-74
Author(s):  
H. D. Nyarko ◽  
G. C. Okpokwasili ◽  
O. F. Joel ◽  
I. A. K. Galyuon
Keyword(s):  
Petroleum Hydrocarbon ◽  
Heterotrophic Bacteria ◽  
Soil Samples ◽  
Cape Coast ◽  
Bacterial Populations ◽  
Isolation And Characterization ◽  
Sampling Locations ◽  
Degrading Bacteria ◽  
Polluted Sites

Aims: The study aimed at the quantification, isolation and characterization of hydrocarbon degrading bacteria in oil-contaminated and pristine soils. Methodology: Soil samples from petroleum hydrocarbon polluted sites at auto-mechanic workshops, a mechanic village, as well as pristine (control) soils, comprising of 14 sampling locations within Cape Coast Metropolis in the Central Region of Ghana were collected using standard sampling techniques. Collected soil samples were treated and cultured while enumerations, isolations and characterization of carbonoclastic bacteria associated were evaluated. Results: Bacterial populations isolated from hydrocarbon-polluted sites had higher aerobic counts ranging from 7.24-8.02 log10 cfu/g of soil when compared with the pristine sites (from 6.79-7.61 log10 cfu/g of soil). Also, soil samples from the mechanic village (8.76 to 7.48 log10 cfu/g of soil) recorded more bacterial counts than those from the mechanic garages (8.02 to 7.24 log10 cfu/g of soil). The calculated percentage profiles of all the hydrocarbon utilizing bacteria in the total culturable heterotrophic bacteria were low throughout the study, even though the percentage scores were all above 50%. A total of 19 hydrocarbon degraders were isolated. The isolates identified belong to the genera Pseudomonas, Proteus, Bacillus and Enterobacter. Conclusion: The outcome of the study based on the bacteria populations, identification profiles, coupled with their survival and multiplications in designated medium amended with crude oil as the carbon and energy sources, suggest their petroleum hydrocarbon degrading capabilities, hence may be used in bioremediation applications.

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