Plant ARGONAUTE Protein Immunopurification  for Pathogen Cross Kingdom Small RNA Analysis

AbstractWe conducted a transcriptomic and small RNA analysis of infective juveniles (IJs) from three behaviourally distinct Steinernema species. Substantial variation was found in the expression of shared gene orthologues, revealing gene expression signatures that correlate with behavioural states. 97% of predicted microRNAs are novel to each species. Surprisingly, our data provide evidence that isoform variation can effectively convert protein-coding neuropeptide genes into non-coding transcripts, which may represent a new family of long non-coding RNAs. These data suggest that differences in neuropeptide gene expression, isoform variation, and small RNA interactions could contribute to behavioural differences within the Steinernema genus.

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De Novo Transcriptome and Small RNA Analysis of Two Chinese Willow Cultivars Reveals Stress Response Genes in Salix matsudana

PLoS ONE ◽

10.1371/journal.pone.0109122 ◽

2014 ◽

Vol 9 (10) ◽

pp. e109122 ◽

Cited By ~ 15

Author(s):

Guodong Rao ◽

Jinkai Sui ◽

Yanfei Zeng ◽

Caiyun He ◽

Aiguo Duan ◽

...

Keyword(s):

Stress Response ◽

Small Rna ◽

De Novo ◽

De Novo Transcriptome ◽

Rna Analysis ◽

Stress Response Genes ◽

Salix Matsudana

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Assembly and function of small RNA – Argonaute protein complexes

Biological Chemistry ◽

10.1515/hsz-2014-0116 ◽

2014 ◽

Vol 395 (6) ◽

pp. 611-629 ◽

Cited By ~ 42

Author(s):

Anne Dueck ◽

Gunter Meister

Keyword(s):

Gene Expression ◽

Gene Silencing ◽

Small Rna ◽

Small Rnas ◽

Atp Hydrolysis ◽

Protein Complexes ◽

Protein Family ◽

Argonaute Protein ◽

Argonaute Proteins ◽

And Function

Abstract Small RNAs such as microRNAs (miRNAs), short interfering RNAs (siRNAs) or Piwi-interacting RNAs (piRNAs) are important regulators of gene expression in various organisms. Small RNAs bind to a member of the Argonaute protein family and are incorporated into larger structures that mediate diverse gene silencing events. The loading of Argonaute proteins with small RNAs is aided by a number of auxiliary factors as well as ATP hydrolysis. This review will focus on the mechanisms of Argonaute loading in different organisms. Furthermore, we highlight the versatile functions of small RNA-Argonaute protein complexes in organisms from all three kingdoms of life.

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Small RNA-mediated genomic silencing promotes telomere stability in the absence of telomerase

10.1101/292722 ◽

2018 ◽

Author(s):

Charlie Longtine ◽

Stephen Frenk ◽

Shawn Ahmed

Keyword(s):

Dna Damage ◽

Caenorhabditis Elegans ◽

Dna Damage Response ◽

Small Rna ◽

Small Rnas ◽

Argonaute Protein ◽

Heterochromatin Formation ◽

Damage Response ◽

The Stability ◽

Telomere Erosion

AbstractTelomerase deficiency in human somatic cells results in telomere erosion and senescence. Small RNAs that target telomeres have been observed in diverse organisms but their functions are not well characterized. We define an endogenous small RNA pathway in Caenorhabditis elegans that promotes heterochromatin formation at telomeres via Dicer, the perinuclear Argonaute protein WAGO-1 and the nuclear Argonaute protein HRDE-1. Loss of telomerase induces biogenesis of siRNAs that target the telomeric lncRNA TERRA, whereas loss of both telomerase and small RNA-mediated telomeric silencing induces TERRA expression, DNA damage, and an accelerated sterility phenotype. These phenotypes can be rescued by exogenous telomeric siRNAs or by loss of the DNA damage response protein EXO-1. Thus, endogenous siRNAs interact with TERRA to promote heterochromatin formation in a manner that is critical for the stability of naturally eroding telomeres. We propose that small RNA-mediated genome silencing could be broadly relevant to regulation of proliferative aging.

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