scholarly journals Genetic Diversity of Dacrycarpus imbricatus At Bukit Tapak, Tabanan, Bali Based on RAPD Marker

2021 ◽  
Vol 9 (2) ◽  
pp. 157-161
Author(s):  
Made Pharmawati ◽  
I Made Anom Sutrisna Wijaya
Keyword(s):  
Genetic Diversity ◽  
Rapd Marker

scholarly journals Analysis of Genetic Diversity of Indonesia Rodent Tuber (Typhonium flagelliforme Lodd.) Cultivars Based on RAPD Marker

2015 ◽  
Vol 2 (1) ◽  
pp. 139
Author(s):  
Danny Laurent ◽  
Nesti F. Sianipar ◽  
Chelen _ ◽  
Listiarini _ ◽  
Ariandana Wantho
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Medicinal Plant ◽  
Genetic Distance ◽  
Genetic Information ◽  
Rapd Marker ◽  
Anti Cancer ◽  
Cancer Agent

<p>Rodent tuber (Typhonium flagelliforme Lodd.) is a plant from Araceae family. The plant has high medical potential as anti-cancer agent. The information regarding Indonesian rodent tuber’s genetic diversity is not available yet. Genetic information is very important for the development of rodent tuber as medicinal plant. In this research, genetic diversity and genetic distance of three Indonesian rodent tuber’s cultivars, from Bogor, Pekalongan, and Medan, were analyzed by using RAPD molecular markers. The data obtained was analyzed by NTsys software. Out of 16 primers used in the study, the 12 primers were found to be polymorphic. There were 83 bands of DNA obtained and 31 of them were polymorphic. Dendogram analysis of the three rodent tuber cultivars showed that these cultivars were clustered into two clusters. The first cluster consists of rodent tuber Bogor and Medan. The second cluster consists of rodent tuber Pekalongan. The coefficient of similarity ranged from 0.81 to 0.87. The highest coefficient of similarity was 0.87, which was detected between rodent tuber Pekalongan and Medan. The lowest coefficient of similarity was 0.81, which was detected between rodent tuber Bogor and Pekalongan. Among these three cultivars of rodent tuber, cultivar Bogor was exclusively different.</p><p><br /><strong>Keywords</strong>: Indonesia-rodent tuber, genetic diversity, RAPD-marker</p>

scholarly journals Analysis of Genetic Diversity of 12 Genotypes of Glycine max by Using RAPD Marker

2017 ◽  
Vol 6 (7) ◽  
pp. 656-663 ◽  
Author(s):  
Shashank A. Tidke ◽  
D. Ramakrishna ◽  
S. Kiran ◽  
Georgina Kosturkova ◽  
G.A. Ravishankar
Keyword(s):  
Genetic Diversity ◽  
Glycine Max ◽  
Rapd Marker

scholarly journals Molecular Evaluation of Three Populations of Farafra Sheep in Comparison to Ossimi and Rahmani Sheep Breeds

2020 ◽  
Vol 68 (6) ◽  
pp. 929-936
Author(s):  
Talaat Bashandy ◽  
Ahmed Hussein ◽  
Mohamed Solma ◽  
Ayman Kassab ◽  
Hatem Hamdon
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Rapd Markers ◽  
Rapd Marker ◽  
Sheep Breeds ◽  
Main Cluster ◽  
Ideal Approach ◽  
Different Populations ◽  
The Mean ◽  
Molecular Evaluation

Molecular markers are the most ideal approach to study genetic diversity. Consequently, we utilized both ISSR and RAPD markers to assess genetic diversity and relationships among three different populations of Farafra, Ossimi and Rahmani Egyptian sheep breeds. Both ISSR and RAPD gave moderate polymorphism 41.3% and 48.51%, respectively. Besides, this value was consistent with the moderate value of the mean of polymorphism information content (0.16 and 0.20, respectively). Farafra-F and Farafra-D populations had the highest similarity which was 0.92 for ISSR and 0.90 for the RAPD marker. Furthermore, ISSR and RAPD constructed dendrogram separated all the studied sheep into two main clusters. All the three populations of Farafra breed combined into one main cluster, while the second cluster contained both Rahmani and Ossimi breeds. The used molecular markers were able to discriminate among evaluated sheep and displayed that Farafra breed more closely related to Ossimi than Rahmani breed.

scholarly journals Genetic diversity and productivity of Gracilaria coronopifolia as alternative for food resource based on RAPD marker

2019 ◽  
Vol 20 (12) ◽  
Author(s):  
Gut Windarsih ◽  
DWINITA WIKAN UTAMI ◽  
SITI YURIYAH
Keyword(s):  
Genetic Diversity ◽  
Rapd Marker ◽  
Food Resource ◽  
Dry Weight ◽  
Selection Marker ◽  
Genetic Profile ◽  
High Productivity ◽  
Band Size ◽  
Three Samples ◽  
Group B

Abstract. Windarsih G, Utami DW, Yuriyah S. 2019. Genetic diversity and productivity of Gracilaria coronopifolia as alternative for food resource based on RAPD marker. Biodiversitas 20: 3758-3765. Gracilaria coronopifolia is one of potential seaweed as an alternative for food resources, especially for agar source. The information about the genetic profile of G. coronopifolia will be useful for assisting in conservation and breeding efforts. RAPD marker is expected to be utilized for characterization of the species with high productivity based on genetic profile. This research aims to determine the genetic diversity and productivity of G. coronopifolia based on RAPD marker. The samples were collected from Anyer Beach, Banten, Indonesia i.e. Tanjung Tum Beach, Mambruk Hotel Beach, and Patra Comfort Anyer Beach, as well as the cultivation ponds of seaweed in Lontar Village, Banten, Indonesia. Five RAPD primers (OPA-01, OPA-16, OPT-05, OPR-02, and UBC-594) were screened to characterize the genetic profile of samples. The results showed that all primers produced polymorphic bands. Based on the genetic profile, the samples of G. coronopifolia were separated into two main clusters, consisted of group A (Tanjung Tum Beach, Patra Beach, and Mambruk Beach) with a coefficient of similarity of 0.44 and group B (Lontar Village) with a similarity coefficient of 0.43. The sample from Lontar Village was separated outside from the other three samples at the waters of Anyer Beach. The sample from Lontar Village had the highest talus height (18.0 cm) and dry weight (1.250 g/clump). The UBC-594 primer was the most associated with the talus height and dry weight on the band size of 50 bp, thus this primer was assumed can be used as a selection marker for the talus height and dry weight of G. coronopifolia.

scholarly journals Characterization of genetic diversity of cactus species (Opuntia spp.) in Morocco by morphological traits and molecular markers

2017 ◽  
Vol 5 (2) ◽  
pp. 149-159 ◽  
Author(s):  
Y. El Kharrassi ◽  
M.A. Mazri ◽  
M.H. Sedra ◽  
A. Mabrouk ◽  
B . Nasser ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Morphological Traits ◽  
Polymorphic Information Content ◽  
Random Amplified Polymorphic Dna ◽  
Rapd Marker ◽  
Group Method ◽  
Rapd Primer ◽  
Opuntia Spp ◽  
Issr Primers

The genetic diversity within and among 124 accessions of Opuntia spp. collected from different regions of Morocco was assessed using morphological descriptors and molecular markers. Based on 10 morphological traits, the accessions were separated into 3 main clusters; each cluster was containing accessions from different regions and species. Polymerase chain reaction (PCR) was then performed on 22 accessions from different regions and species, with 10 inter-simple sequence repeat (ISSR) primers and one random amplified polymorphic DNA (RAPD) primer. ISSR primers produced 66 bands overall, 64 (96.9 %) of which were polymorphic while 6 bands were generated by the RAPD marker, all polymorphic. The polymorphic information content (PIC) values ranged from 0.62 to 0.97, with an average of 0.82. The dendrogram of genetic differences generated using the unweighted pair-group method using arithmetic averages (UPGMA) method showed 7 different clusters at a similarity of 0.76, which was confirmed by the principal component analysis (PCA). The main conclusion of our work is the high genetic similarity between Opuntia ficus indica and Opuntia megacantha species in Morocco. Our results will be useful for plant breeding and genetic resource conservation programs.

scholarly journals The Study of the genetic diversity of two laccate species of Ganoderma lucidum and Ganoderma resinaceum using RAPD marker

2019 ◽  
Vol 5 (4) ◽  
pp. 379-388
Author(s):  
Somayeh Keypour ◽  
Hossein Riahi ◽  
Mostafa Ebadi ◽  
Ali Borhani ◽  
Mohammad Reza Asef Shayan ◽  
...  
Keyword(s):  
Genetic Diversity ◽  
Ganoderma Lucidum ◽  
Rapd Marker
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