scholarly journals KAJIAN POTENSI ANTIOKSIDAN ALAMI BERBAGAI LIMBAH TANAMAN

2021 ◽  
Vol 26 (1) ◽  
pp. 57-63
Author(s):  
Rukiah Lubis ◽  
Nur Hidayah ◽  
Nopriyeni Nopriyeni
Keyword(s):  
Vitamin A ◽  
Vitamin C ◽  
High Performance ◽  
Natural Antioxidants ◽  
Natural Antioxidant ◽  
Anthocyanin Content ◽  
Banana Plant ◽  
Melastoma Malabathricum ◽  
Materials Used

Tujuan dari penelitian ini adalah untuk menganalisis kandungan antioksidan (antosianin, vitamin A, C, dan E) dari limbah kulit buah senduduk (Melastoma malabathricum L.), jantung pisang, jengkol. Penelitian dilaksanakan selama enam bulan. Analisa antosianin dan vitamin A, C, dan E dilakukan di Balai Besar Industri Agro, Bogor. Bahan yang digunakan pada penelitian ini terdiri dari kulit buah senduduk, jantung pisang, dan jengkol. Analisis kandungan vitamin A dilakukan pada sampel mengunakan alat High Performance Liquid Cromatography (HPLC). Penentuan  kadar  antosianin  dihitung menggunakan metode perbedaan pH. Data yang diperoleh dianalisis secara deskriptif. Hasil penelitian menunjukkan bahwa aktivitas antioksidan alami yang paling tinggi menurut analisis kandungan vitamin A, C, E dan antosianin dari ketiga limbah tanaman (buah senduduk, jantung pisang, kulit jengkol), terdapat pada kulit buah senduduk dan kulit jantung pisang yaitu kandungan vitamin C (111.33 mg/kg dan 263.33 mg/kg) dan kandungan antosianin (684.008 mg/100g dan 174.289 mg/100g). Dapat disimpulkan bahwa buah senduduk dan jantung pisang bisa dijadikan sebagai antioksidan alami yang baik berdasarkan hasil analisis kandungan vitamin A, C, E, dan kandungan antosianin.THE STUDY OF POTENTIAL NATURAL ANTIOXIDANTS FROM PLANT WASTEThis study was aimed at analyzing the content of antioxidants (anthocyanins, vitamins A, C, and E) from the rind of sanduduk’s fruit (Melastoma malabathricum L.), Blossom of the banana plant (Musa acuminata Colla), jengkol (Archidendron pauciflorum) fruit. The research was conducted for six months. Analysis of anthocyanins and vitamins A, C, and E was performed at Balai Besar Industri Agro, Bogor. The materials used in this research consisted of sanduduk rind, banana blossom, and jengkol fruit. Vitamin A content analysis was performed on the samples using High Performance Liquid Chromatography (HPLC). The determination of anthocyanin levels was calculated using the pH difference method. The data obtained were analyzed descriptively. The results showed that the highest natural antioxidant activity according to the analysis of vitamin A, C, E and anthocyanin content from the three wastes, found in sanduduk rind and banana blossom which contain vitamin C ( 111.33 mg/kg and 263.33 mg/kg) and anthocyanin (684.008 mg/100g and 174.289 mg/100g). It can be concluded that sanduduk fruit and banana blossom can be used as a good natural antioxidant based on the results of analysis of vitamin A, C, E, and anthocyanin content.

scholarly journals Evaluation of different methodologies for the determination of phenolic compounds in tropical fruits

2020 ◽  
Vol 23 ◽  
Author(s):  
Karina Carvalho Guimarães ◽  
Derlyene Lucas Salgado ◽  
Elisângela Elena Nunes Carvalho
Keyword(s):  
Phenolic Compounds ◽  
Vitamin C ◽  
Fruits And Vegetables ◽  
Titratable Acidity ◽  
Natural Antioxidants ◽  
Soluble Solids ◽  
Total Phenolic ◽  
Fast Blue ◽  
Recent Method

Abstract Phenolic compounds are natural antioxidants and can be found with abundance in fruits and vegetables. One of the first methods created for analyzing phenolic compounds was the Folin-Denis method; and hereafter, the Folin Ciocalteu method was developed and recently, one of the most recent method is the Fast Blue. Due to the importance of these compounds in food, this work aimed to determine the total phenolic compounds in three fruits, such as: Passion Fruit, “Palmer” mango and “Pera” orange. In addition, these fruits were analyzed through three different determination methods. Furthermore, the content of vitamin C, soluble solids, titratable acidity and pH of these fruits were evaluated. Based on the results, it was verified that there was no difference among the values of vitamin C, soluble solids and TA in relation to those found in the literature. When comparing the three methods (Fast Blue BB, Folin-Ciocalteu Reagent and Folin-Denis Reagent), the results showed a difference in the phenolic compounds that may be related to the distinct reagents used in each method and its different action.

Simultaneous determination of retinol and retinyl esters in serum or plasma by reversed-phase high-performance liquid chromatography.

1978 ◽  
Vol 24 (11) ◽  
pp. 1920-1923 ◽  
Author(s):  
M G DeRuyter ◽  
A P De Leenheer
Keyword(s):  
Vitamin A ◽  
High Performance ◽  
Chromatographic Determination ◽  
Reversed Phase ◽  
Retinyl Palmitate ◽  
Serum Retinol ◽  
Liquid Chromatographic Determination ◽  
Retinyl Esters ◽  
Liquid Chromatographic

Abstract We propose a single-run liquid-chromatographic determination, with ultraviolet detection at 330 nm, for serum retinol and retinyl esters. The vitamin A derivatives are extracted according to the Bligh-Dyer procedure. With 200 microliter or serum, the lower detection limit is 50 microgram/liter for retinol and about 100 microgram/liter for retinyl esters. Within-run precision (CV) was 2.3% for retinol, 4.3% for retinyl palmitate. Day-to-day percision (CV, n = 20) for retinol was 4.9% during a month. The method can be used for the assessment of vitamin A absorption tests and for the determination of serum retinol (normal, subnormal, and above-normal concentrations). Serum retinyl esters can only be measured in conditions where concentrations exceed 100 microgram/liter.

scholarly journals Analysis of Vitamin A in Multivitamin Products

2021 ◽  
Vol 11 (3) ◽  
pp. 174-184
Author(s):  
A. S. Alekseeva ◽  
T. B. Shemeryankina ◽  
M. N. Lyakina ◽  
M. S. Smirnova ◽  
E. P. Fedorova ◽  
...  
Keyword(s):  
Vitamin A ◽  
High Performance ◽  
Beta Carotene ◽  
Test Methods ◽  
Retinyl Palmitate ◽  
Test Method ◽  
Medicinal Products ◽  
Retinyl Acetate ◽  
Preliminary Purification

Vitamin A is present in multivitamin products mainly in the form of retinol esters: retinyl acetate, retinyl palmitate, and beta carotene—retinol precursor (dimer) found in plants, which is capable of converting into retinol in liver cells. Retinol is determined in medicinal products primarily by high performance liquid chromatography (HPLC), with preliminary purification and vitamin isolation by liquid-liquid extraction. However, scientific literature also describes other methods of sample preparation and analysis of such compounds. An important issue is differentiation of vitamin A from other fat-soluble vitamins often included as components in multivitamin products. The aim of the study was to analyse and summarise data on current methods used for determination of vitamin A and its derivatives in medicinal products. The authors analysed the range of vitamin A products authorised in the Russian Federation, and the test methods described in their product specification files. The study demonstrated that the test method most often used for determination of retinol esters was HPLC with isocratic elution mode using octadecylsilyl packing in the reverse-phase mode, and, less frequently, aminopropylsilyl packing in the normal phase mode. Determination of beta carotene in medicinal products is most often performed using spectrophotometry. 

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