Production of Fuel Ethanol from Hydrotherm-Pretreated Corn Stover by Pichia stipitis

2013 ◽  
Vol 641-642 ◽  
pp. 943-946
Author(s):  
Qiang Zhang
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Substrate Concentration ◽  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Fuel Ethanol ◽  
Hydrothermal Pretreatment ◽  
Raw Material ◽  
Initial Ph ◽  
Simultaneous Saccharification

Corn stover is an abundant raw material for fuel ethanol production. Finding out the appropriate process for ethanol production will be possible to achieve industrilization. Hydrothermal pretreatment (195°C,15min) and simultaneous saccharification and fermentation (SSF) were adopted to produce ethanol from corn stover. The results showed that 86.5% of cellulose were remained in solid cake and most of hemicellulose were solubilized after pretreatment. The highest ethanol concentrationl of 12.12 g/ L was achieved at initial pH of 5.5，temperature 30°C and 130 rpm shaking rate with substrate concentration of 5%(w/v) by Pichia stipitis58376 after 192 h.The corresponding volumetric productivity were 0.065g/Lh .

Ethanol Production from Corn Stover Using Soaking Pretreatment

2010 ◽  
Vol 171-172 ◽  
pp. 261-265
Author(s):  
Zhuang Zuo ◽  
Xiu Shan Yang
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Pichia Stipitis ◽  
Liquid Ratio ◽  
Mild Conditions ◽  
Whole Process ◽  
Conversion Rates ◽  
Mild Temperature ◽  
Simultaneous Saccharification

Corn stover was pretreated using different soaking conditions at mild temperature. Among the tested conditions, the best was 1% NaOH+8% NH4OH，50°C，48 h, Solid-to-liquid ratio 1:10. The results showed that soaking pretreatment achieved 63.6% delignification, retained the xylan and glucan. After enzymatic hydrolysis, conversion rates of xylan and glucan were 70.9% and 78.5%, respectively. The pretreated filtration re-soaking cause 52.7% xylan and 65.0% glucan conversion. NaOH+NH4OH treatment can be performed under mild conditions, gives a good buffering effect, low carbohydates degradation and extensive removal of lignin. Additionally, simultaneous saccharification and fermentation was conducted with pretreated corn stover to assess the ethanol production. For the whole process, 0.15g ethanol /g corn stover was achieved using Saccharomyces cerevisiae Y5, and 0.19g ethanol /g corn stover when using Pichia stipitis.

Fuel Ethanol Production from Wet Oxidised Corn Stover by S. Cerevisiae

2011 ◽  
Vol 343-344 ◽  
pp. 963-967 ◽  
Author(s):  
Zhang Qiang ◽  
Anne Belinda Thomsen
Keyword(s):  
Ethanol Production ◽  
Corn Stover ◽  
Simultaneous Saccharification And Fermentation ◽  
Ethanol Concentration ◽  
Ethanol Yield ◽  
Liquid Fraction ◽  
Raw Material ◽  
Wet Oxidation ◽  
Inhibition Effect ◽  
Simultaneous Saccharification

In order to find out appropriate process for ethanol production from corn stover, wet oxidation（195°C，15 minutes）and simultaneous saccharification and fermentation (SSF) was carried out to produce ethanol. The results showed that the cellulose recovery of 92.9% and the hemicellulose recovery of 74.6% were obtained after pretreatment. 86.5% of cellulose was remained in the solid cake . After 24h hydrolysis at 50°C using cellulase（Cellubrix L）,the achieved conversion of cellulose to glucose was 64.8%. Ethanol production was evaluated from dried solid cake and the hydrolysate was employed as liquid fraction . After 142 h of SSF with substrate concentration of 8% (W/V), ethanol yield of 73.1 % of the theoretical based on glucose in the raw material was obtained by S. cerevisiae(ordinary baker’ yeast) . The corresponding ethanol concentration and volumetric productivity were 17.2g/L and 0.121g/L.h respectively. The estimated total ethanol production was 257.7 kg/ton raw material by assuming consumption of both C-6 and C-5. No obvious inhibition effect occurred during SSF. These instructions give you the basic guidelines for preparing papers for WCICA/IEEE conference proceedings.

Optimization of Simultaneous Saccharification and Fermentation for Ethanol Production from Steam-Exploded Cotton Stalk

2013 ◽  
Vol 724-725 ◽  
pp. 391-398
Author(s):  
Qin Zhang ◽  
Yan Bin Li ◽  
Zhan Wen Liu ◽  
Yun Feng Pu ◽  
Li Ming Xia
Keyword(s):  
Ethanol Production ◽  
Simultaneous Saccharification And Fermentation ◽  
Ph Value ◽  
Raw Material ◽  
Cotton Stalk ◽  
Fermentation Time ◽  
Box Behnken Design ◽  
Initial Ph ◽  
Experimental Yield ◽  
Simultaneous Saccharification

Steam-exploded cotton stalk was used as raw material in ethanol production through simultaneous saccharification and fermentation by Penicillium Q59 and Saccharomyces cerevisiae P1. The fermentative conditions were firstly examined by single factor experiments to determine the central point in Box-Behnken design, which was explored with expectation to get optimized fermentative conditions for enhancement of ethanol production. The results of optimized fermentative conditions were determined as follows: fermentation time was 10.5 days, bran added percent was 15%, initial pH value was 5.5. Under the optimal conditions, the experimental yield of ethanol was 99.85 ± 4.21 g·kg-1SECS (steam-exploded cotton stalk), which was close to the theoretical predicting value, it showed the model was feasible. The research results will provide technical reference for further exploitation of cotton stalk.

scholarly journals Effect of Various Pretreatment Methods on Bioethanol Production from Cotton Stalks

Fermentation ◽  
2019 ◽  
Vol 5 (1) ◽  
pp. 5 ◽  
Author(s):  
Konstantinos Dimos ◽  
Thomas Paschos ◽  
Argiro Louloudi ◽  
Konstantinos G. Kalogiannis ◽  
Angelos A. Lappas ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Substrate Concentration ◽  
Ethanol Yield ◽  
Direct Conversion ◽  
Hydrothermal Pretreatment ◽  
Solid Loading ◽  
Hydrolysis Time ◽  
Cotton Stalks ◽  
Sequential Combination ◽  
Simultaneous Saccharification

Cotton stalks (CS) are considered a good candidate for fuel-ethanol production due to its abundance and high carbohydrate content, but the direct conversion without pretreatment always results in extremely low yields due to the recalcitrant nature of lignocelluloses. The present study was undertaken to investigate the effect of various chemical and physicochemical pretreatment methods, i.e., alkali, microwave-assisted acid, organosolv, hydrothermal treatment, and sequentially organosolv and hydrothermal pretreatment, on chemical composition of CS and subsequent ethanol production applying pre-hydrolysis and simultaneous saccharification and fermentation (PSSF) at high solid loading. The best results in terms of ethanol production were achieved by the sequential combination of organosolv and hydrothermal pretreatment (32.3 g/L, using 15% w/v substrate concentration and 6 h pre-hydrolysis) with an improvement of 32% to 50% in ethanol production compared to the other pretreatments. Extending pre-hydrolysis time to 14 h and increasing substrate concentration to 20% w/v, ethanol production reached 47.0 g/L (corresponding to an ethanol yield of 52%) after 30 h of fermentation.

scholarly journals Optimisation of Simultaneous Saccharification and Fermentation (SSF) for Biobutanol Production Using Pretreated Oil Palm Empty Fruit Bunch

Molecules ◽  
2018 ◽  
Vol 23 (8) ◽  
pp. 1944 ◽  
Author(s):  
Nur Md Razali ◽  
Mohamad Ibrahim ◽  
Ezyana Kamal Bahrin ◽  
Suraini Abd-Aziz
Keyword(s):  
Oil Palm ◽  
Substrate Concentration ◽  
Clostridium Acetobutylicum ◽  
Simultaneous Saccharification And Fermentation ◽  
Consolidated Bioprocessing ◽  
Empty Fruit Bunch ◽  
Initial Ph ◽  
Central Composite ◽  
Simultaneous Saccharification ◽  
Clostridium Acetobutylicum Atcc 824

This study was conducted in order to optimise simultaneous saccharification and fermentation (SSF) for biobutanol production from a pretreated oil palm empty fruit bunch (OPEFB) by Clostridium acetobutylicum ATCC 824. Temperature, initial pH, cellulase loading and substrate concentration were screened using one factor at a time (OFAT) and further statistically optimised by central composite design (CCD) using the response surface methodology (RSM) approach. Approximately 2.47 g/L of biobutanol concentration and 0.10 g/g of biobutanol yield were obtained after being screened through OFAT with 29.55% increment (1.42 fold). The optimised conditions for SSF after CCD were: temperature of 35 °C, initial pH of 5.5, cellulase loading of 15 FPU/g-substrate and substrate concentration of 5% (w/v). This optimisation study resulted in 55.95% increment (2.14 fold) of biobutanol concentration equivalent to 3.97 g/L and biobutanol yield of 0.16 g/g. The model and optimisation design obtained from this study are important for further improvement of biobutanol production, especially in consolidated bioprocessing technology.

Evaluation of fuel ethanol production from aqueous ammonia-treated rice straw via simultaneous saccharification and fermentation

2016 ◽  
Vol 93 ◽  
pp. 150-157 ◽  
Author(s):  
Paripok Phitsuwan ◽  
Chutidet Permsriburasuk ◽  
Rattiya Waeonukul ◽  
Patthra Pason ◽  
Chakrit Tachaapaikoon ◽  
...  
Keyword(s):  
Ethanol Production ◽  
Rice Straw ◽  
Simultaneous Saccharification And Fermentation ◽  
Aqueous Ammonia ◽  
Fuel Ethanol ◽  
Simultaneous Saccharification
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