scholarly journals Monitoring Caries Risks before the Window of Infection and Later Caries Increment: A Caries Prediction Study on Rapid Detection of Streptococcus mutans Using Monoclonal Antibodies

2014 ◽  
Vol 55 (1) ◽  
pp. 19-23 ◽  
Author(s):  
Yoshinobu Maki ◽  
Takaharu Sakayori ◽  
SoIchiro Hirata ◽  
Takuo Ishii ◽  
Tachino Atsushi
Keyword(s):  
Monoclonal Antibodies ◽  
Streptococcus Mutans ◽  
Rapid Detection ◽  
Caries Increment ◽  
Caries Prediction

The Capacity of a Specific Anti-Streptococcus Mutans Monoclonal Antibodies Test to Identify the Diabetic Patients with Increased Risk of Periodontal Disease

2017 ◽  
Vol 68 (11) ◽  
pp. 2556-2559
Author(s):  
Mona Ionas ◽  
Sebastian Ioan Cernusca Mitariu ◽  
Adela Dancila ◽  
Tiberiu Horatiu Ionas ◽  
Raluca Monica Comaneanu ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Periodontal Disease ◽  
Streptococcus Mutans ◽  
Mutans Streptococci ◽  
Diabetic Patients ◽  
A Value ◽  
Salivary Level ◽  
Increased Risk ◽  
The Status

By means of a specific anti-Streptococcus mutans monoclonal antibodies test we want to identify the diabetic patients which have an increased risk to develop the periodontal disease. The highest percentage, of 88.1% of all patients included in this study represents the subjects with a level greater than 500,000 cfu / mL of streptococcus mutans. The Kruskal-Wallis test reveals a value of p = 0.283 resulted from the status of diabetes in patients and the level of streptococcus mutans in saliva. In conclusion, the status of diabetes in patients seems not to influence the salivary level of mutans streptococci determined with the method used in our study.

Rapid detection of human rotavirus strains in stools by single-sandwich enzyme-linked immunosorbent assay systems using monoclonal antibodies

1989 ◽  
Vol 24 (1-2) ◽  
pp. 43-56 ◽  
Author(s):  
Giuseppe Gerna ◽  
Antonella Sarasini ◽  
Angela Di Matteo ◽  
Maurizio Parea ◽  
Maria Torseilini ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Immunosorbent Assay ◽  
Rapid Detection ◽  
Enzyme Linked Immunosorbent Assay ◽  
Human Rotavirus

Rapid detection of infectious cytomegalovirus in blood with the aid of monoclonal antibodies

1987 ◽  
Vol 23 (1) ◽  
pp. 31-40 ◽  
Author(s):  
Jurjen Schirm ◽  
Wilma Timmerije ◽  
Wim van der Bij ◽  
T. Hauw The ◽  
Jan B. Wilterdink ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Rapid Detection

Rapid Detection of Salmonella spp. in Foods by Combination of a New Selective Enrichment and a Sandwich ELISA Using Two Monoclonal Antibodies against Dulcitol 1-Phosphate Dehydrogenase

1996 ◽  
Vol 59 (11) ◽  
pp. 1158-1163 ◽  
Author(s):  
HUAIZE TIAN ◽  
TAKAHISA MIYAMOTO ◽  
TAKASHI OKABE ◽  
YOICHIRO KURAMITSU ◽  
KEN-ICHI HONJOH ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Rapid Detection ◽  
Enrichment Culture ◽  
Sandwich Elisa ◽  
False Negative ◽  
Detection Sensitivity ◽  
Elisa Method ◽  
Salmonella Spp ◽  
Selective Enrichment ◽  
Raw Foods

A rapid-detection method was developed for food-borne dulcitol-positive Salmonella spp. in foods that involves a new preenrichment and selective enrichment system and a sandwich ELISA using two monoclonal antibodies against dulcitol 1-phosphate dehydrogenase. Preenrichment and selective enrichment were in Enterobacteriaceae enrichment mannitol (EEM) broth at 42°C for 6 h and in a new dulcitol-magnesium chloride-pyridinesulfonic acid brilliant green-novobiocin (DMPBN) medium at 42°C for 27 h, respectively. The cells were collected from the selective enrichment culture and suspended in 0.1 ml of 1 N NaOH for 2 min. The solution was neutralized with 0.1 ml of 2 M Tris-HCl buffer (pH 7.5) and the mixture was used as a sample for ELISA. The detection sensitivity of the ELISA was 105 CFU of Salmonella spp. per ml of culture. Competing non-Salmonella organisms in raw food did not interfere with the detection of Salmonella cells even when present at 107: 1 (non-Salmonella: Salmonella ratio) in food. Nonmotile Salmonella gallinarum was detected by the ELISA. The minimum detectable number of initial inoculum of Salmonella typhimurium was 0.69 CFU/25 g of raw chicken after the preenrichment in EEM broth and the selective enrichment in DMPBN medium. The present ELISA method required a total analysis time of 36 h including the preenrichment and selective enrichment periods. The ELISA method was compared with a conventional cultural method for the detection of Salmonella cells in 130 samples of raw foods. Of the samples tested, 16 were Salmonella-positive and 114 samples were negative by both methods. False-positive and false-negative results were not encountered.

scholarly journals Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification

2020 ◽  
Vol 605 ◽  
pp. 113812
Author(s):  
Masae Kitagawa ◽  
Kentaro Nagamine ◽  
Hiroko Oka ◽  
Kazuhisa Ouhara ◽  
Ikuko Ogawa ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Rapid Detection ◽  
Isothermal Amplification ◽  
Loop Mediated Isothermal Amplification
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