Composition of Mitochondrial DNA 16S Nucleotide of Dwarf Snakehead  (Channa gachua Hamilton, 1822) from Keji River, Magelang, Central Java

Indonesia has a high marine and freshwater biodiversity including freshwater fish biodiversity. One of freshwater fish which is commonly consumed by Indonesian people is dwarf snakehead (Channa gachua Hamilton, 1822). However, research on genetic characterization, especially the composition of mtDNA 16S nucleotide of dwarf snakehead has poorly understood. Therefore, the aim of this study was to determine the composition of mtDNA 16S nucleotide of dwarf snakehead as a part of genetic characterization of the fish species taken from Keji River, Magelang, Central Java which has not been previously examined. This study analyzed 16S mt-DNA of two samples of dwarf snakehead from Keji River (KTS-01 and KTS-02). In addition, two sequences of Channa gachua with accession number KU986900, KU238074, and HM117234-HM117238 taken from GenBank were used as a comparison. A method used in this research was a PCR method and primers used in this research were 16Sar and 16Sbr. The results revealed that the average of nucleotide composition T, C, A and G of the fish species was 23.04%, 25.13%, 29.06% and 22.77% respectively whereas the average rate of nucleotide composition A+ T and G+ C was 52.10% and 47.90% respectively. The two dwarf snakehead had similar T and C composition but different in A and G composition. In addition, the G+C content in KTS-01 and KTS-02 had the highest frequency compared to other dwarf snakehead taken from GenBank. From this finding it could be assumed that there is genetic variation between the two dwarf snakehead from Keji River which is important genetic data for breeding program of the fish species in the future.

Download Full-text

The 16S and COI Mitochondrial DNA Nucleotide Composition of Stripped Snakehead (Channa striata Bloch,1793) from Lake Sentani, Jayapura, Papua

Jurnal Perikanan Universitas Gadjah Mada ◽

10.22146/jfs.35551 ◽

2019 ◽

Vol 20 (2) ◽

pp. 57

Author(s):

Christine Bawaeda Sitandung Kombong ◽

Tuty Arisuryanti

Keyword(s):

Mitochondrial Dna ◽

Fish Species ◽

Nutritional Value ◽

Genetic Characterization ◽

Mitochondrial Gene ◽

Nucleotide Composition ◽

Omega 3 ◽

Channa Striata ◽

Pcr Method

Stripped snakehead (Channa striata Bloch,1793) has potency to be developed in Indonesia due to high nutritional value including omega 3 which is useful for children growth. However, study on composition of mitochondrial DNA nucleotide as a part of study on genetic characterization of stripped snakehead fish in Indonesia is poorlyunderstood. Genetic characterization of stripped snakehead is important to obtain data information that can be applied for breeding and conservation program. Therefore, the objective of this study was to determine the 16S and COI mitochondrial DNA nucleotide of stripped snakehead collected from Lake Sentani, Jayapura, Papua. Method used in this research is PCR method using primer 16Sar and 16Sbr for 16S mitochondrial gene, and FishF2 and FishR2 for COI mitochondrial gene. The results showed that composition of 16S mtDNA nucleotide from 616 bp was T=21.92%, C=25.49%, A=30.19%, dan G=22.40%, whereas composition of A+T=52.11% and G+C=47.89%. In addition, composition of COI mtDNA nucleotide from 705 bp was T=29.93%, C=28.65%, A=24.26%, and G=17.16% while composition of A+T=54.48% and G+C=45.52%. Data of 16S and COI mitochondrial DNA nucleotide composition of the stripped snakehead collected from Lake Sentani gained in this study are to be expected to complete genetic information of the fish species which is useful to improve breeding program of this fish species in the future.

Download Full-text

Freshwater fish diversity in three tributary streams in Serayu Basin, Central Java, Indonesia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d211244 ◽

2020 ◽

Vol 21 (12) ◽

Author(s):

Suhestri Suryaningsih ◽

Dian Bhagawati ◽

Sri Sukmaningrum ◽

Sugiharto Sugiharto ◽

Ayu ratna I gusti agung

Keyword(s):

Freshwater Fish ◽

Fish Species ◽

Fish Diversity ◽

Random Group ◽

Freshwater Fish Species ◽

Central Java ◽

Gill Net ◽

Southern Central ◽

Temporal And Spatial ◽

Fish Species Diversity

Abstract. Suryaningsih S, Bhagawati D, Sukmaningrum S, Sugiharto, Puspitasari IGAAR. 2020. Freshwater fish diversity in three tributary streams in Serayu Basin, Central Java, Indonesia. Biodiversitas 21: 5811-5817. Determining the diversity of freshwater fish species in Central Java, Indonesia has been confounded by temporal and spatial limitations in past studies. The Serayu Basin is a large watershed in Central Java that is believed to have high freshwater fish diversity. We aimed to determine the diversity and community structure of freshwater fish species in three tributaries of the Serayu, elucidate the factors influencing this diversity, and determine the extent to which these tributaries contribute to the total freshwater fish species richness for southwest Central Java. We conducted gill net sampling from May to August 2018 on the Tulis, Mrawu, and Kali Sapi Rivers located at Banjanegara district Sampling followed a random group technique in upstream, midstream, and downstream river sections of each tributary. In total, we observed 21 freshwater fish species. Broadly, fish species diversity was relatively low in the study area, and water temperature and dissolved oxygen were important in maintaining fish diversity. These three tributaries house 27–46% of the freshwater fish species reported for southern Central Java.

Download Full-text

Freshwater fish distribution in the Maghreb: a call to contribute

10.31219/osf.io/kx4gc ◽

2020 ◽

Author(s):

Matthew Ford ◽

Amina Brahimi ◽

Lyas Baikeche ◽

Laura Bergner ◽

Miguel Clavero ◽

...

Keyword(s):

Freshwater Fish ◽

Fish Species ◽

Iucn Red List ◽

Fish Distribution ◽

Freshwater Biodiversity ◽

Global Biodiversity Information Facility ◽

Geographic Ranges ◽

Freshwater Fish Species ◽

Comprehensive Knowledge ◽

Corrected Data

In order to update the IUCN Red List of all freshwater fish species inhabiting the Maghreb region, comprehensive knowledge of their respective geographic ranges is essential. Here we present site-scale distribution records derived from all known sources for native and alien freshwater fish species distributed in Morocco, Algeria and Tunisia. Corrected data compiled from the Global Biodiversity Information Facility plus author contributions and digitised literature data are presented as GIS maps, and the wider scientific and conservation communities are called upon to help fill knowledge gaps and maximise the value of this new database for a region in which freshwater biodiversity is undergoing a serious decline.

Download Full-text

Identifikasi Keanekaragaman dan Potensi Jenis-Jenis Ikan Air Tawar Pulau Bangka

Akuatik Jurnal Sumberdaya Perairan ◽

10.33019/akuatik.v12i1.694 ◽

2018 ◽

Vol 12 (1) ◽

pp. 74-80

Author(s):

Irma Akhrianti ◽

Andi Gustomi

Keyword(s):

Water Quality ◽

Freshwater Fish ◽

Fish Species ◽

Conservation Value ◽

Economic Potential ◽

Freshwater Biodiversity ◽

Potential Analysis ◽

Freshwater Fish Species ◽

Fish Resources ◽

Fishery Resources

Characteristics of Bangka Island which is an archipelago region with high economic potential of marine and fishery resources, it is deemed necessary to conduct research on the diversity of freshwater fish to support the increase of economic, ecological and conservation value of freshwater fish resources. Because of considering the research especially freshwater biodiversity of fish is seldom doing in Bangka Island, this research need to done. The aim of this research is to compile and identify the diversity of freshwater fish species in Bangka Island and to identify the potential utilization of freshwater fish species in Bangka Island based on the characteristics of dichromaticism and dimorphism. There are 63 species of 24 families of freshwater fish in the waters of Bangka Island. 2 unidentified fish species. Family Cyprinidae is the most caught type of 18 individuals. Fish potential analysis shows that there are 26 species of fish with potential for consumption, 33 species of fish have ornamental potential, 5 types of fish have ornamental potential and consumption, and 1 species of unidentified fish potential. Analysis of water quality in three observation streams indicates that water quality is still good for fish life

Download Full-text

Diversity and longitudinal distribution of freshwater fish in Klawing River, Central Java, Indonesia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d190114 ◽

2018 ◽

Vol 19 (1) ◽

pp. 85-92

Author(s):

SUHESTRI SURYANINGSIH ◽

SRI SUKMANINGRUM ◽

SORTA BASAR IDA SIMANJUNTAK ◽

KUSBIYANTO KUSBIYANTO

Keyword(s):

Cluster Analysis ◽

Freshwater Fish ◽

Fish Species ◽

The Other ◽

Longitudinal Distribution ◽

Number Of Species ◽

Central Java ◽

Key Factor ◽

Single Cluster ◽

Distribution Of Fish

Suryaningsih S, Sukmaningrum S, Simanjuntak SBI, Kusbiyanto. 2018. Diversity and longitudinal distribution of freshwater fish in Klawing River, Central Java, Indonesia. Biodiversitas 19: 85-92. The aims of this study were to evaluate the diversity and longitudinal distribution of fish in Klawing River, Purbalingga (Central Java). The survey was performed using a clustered randomsampling technique. The river was divided into upstream, midstream and downstream regions. Species diversity was measured as the number of species, and the longitudinal distribution was assessed by determining the fish species present in each of the three regions. Eighteen fish species of eleven families were identified in the Klawing River: Cyprinidae, Bagridae, Mastacembelidae, Anabantidae, Cichlidae, Channidae, Eleotrididae, Beleontinidae, Osphronemidae, Poecilidae, and Siluridae. Cyprinidae exhibited the highest number of species (six), followed by Bagridae and Cichlidae (two species each). The other families were represented by one species each. A single cluster analysis showed that the upstream population had a similarity of 78% and 50% with the midstream and downstream populations, respectively. Species and family diversities were higher in the midstream populations than in the upstream and downstream populations. This longitudinal distribution may be due to differences in environmental conditions and suggests that management of the land surrounding the Klawing River is a key factor in the conservation of freshwater fish.

Download Full-text