CYTOTOXIC EFFECTS OF ETHYL ACETATE FRACTIONS FROM SECONDARY METABOLITES OF STREPTOMYCES SP. GMY01 ON HUMAN BREAST CANCER MCF7 CELL LINES

Objective: This research aimed to fractionate the ethyl acetate extract from secondary metabolites of Streptomyces sp. GMY01 and to identify which fraction contains cytotoxic active compounds against human breast cancer MCF7 cell lines.Methods: Secondary metabolites were obtained from fermentation of Streptomyces Sp. GMY01 for 15 d. The supernatant containing these secondary metabolites was extracted through partition using ethyl acetate as the solvent. Fractionation of the ethyl acetate extract was conducted via column chromatography using silica gel as the solid phase while the gradient mobile phase consisted of n-hexane, ethyl acetate, and methanol. The cytotoxicity of each fraction was calculated using MTT-assay.Results: The ethyl acetate extract could be separated into 9 fractions using column chromatography. The cytotoxic effect of each fraction differed from each other. The smallest IC50 value was obtained from fraction 4. Further investigation should be conducted to discover the active anticancer compound. The active compound with cytotoxic effect was found in fraction 4 because of the highest IC50 value.Conclusion: This fraction is potential to be investigated more deeply as anticancer, especially for breast cancer.

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Morphomolecular identification, metabolic profile, anticancer, and antioxidant capacities of Penicillium sp. NRC F1 and Penicillium sp. NRC F16 isolated from Egyptian remote cave

10.21203/rs.3.rs-438364/v1 ◽

2021 ◽

Author(s):

Waill A. Elkhateeb ◽

Walaa S. A. Mettwally ◽

Shireen A. A. Saleh ◽

Walid Fayad ◽

Ibrahim M. Nafady ◽

...

Keyword(s):

Breast Cancer ◽

Colon Cancer ◽

Ethyl Acetate ◽

Human Breast Cancer ◽

Mcf7 Cell ◽

Human Colon ◽

Human Breast ◽

Human Colon Cancer ◽

Breast Cancer Mcf7 Cell

Abstract Searching for remote locations to screen for microorganisms, identify their metabolites, and investigate their bioactivities against lethal diseases such as cancer is of critical importance. In the current study, two fungal strains where isolated from a remote cave in Asyut governorate, Egypt. These isolates were morphologically and molecularly identified through sequencing their ITS region as Penicillium sp. NRC F1, and Penicillium sp. NRC F16. Investigating the metabolic profiles of the silylated ethyl acetate extracts of these fungi through conducting GC-Ms analysis revealed presence of 114 compounds belonging to different chemical classes. On the other hand, studying the in vitro bioactivity of both extracts showed moderate antioxidant activities. Penicillium sp. NRC F1 extract exhibited higher DPPH scavenging activity (74.41 ± 0.59%) at concentration of 200 µg/ml, in comparison with that exerted by Penicillium sp. NRC F16 extract at the same concentration (65.58 ± 1.55%). Moreover, studying the cytotoxicity of extracts against human colon cancer (HCT116), and human breast cancer (MCF7) cell lines revealed that cytotoxicity of both extracts was dose dependent. Promising cytotoxic effect was achieved against human colon cancer HCT116 using 200 µg/ml of Penicillium sp. NRC F1 extract (95.72 ± 1.13 % cytotoxicity), while Penicillium sp. NRC F16 ethyl acetate extract caused a cytotoxicity of 95.43 ± 1.4 %. Similarly, investigating the in vitro cytotoxicity of the extracts against human breast cancer MCF7 cell line resulted in observing promising activity of Penicillium sp. NRC F1 and Penicillium sp. NRC F16 extracts, and they exhibited 97.29 ± 0.61 %; and 97.08 ± 1.07 % cytotoxicity, respectively. Results from this study nominate those strains as promising isolates and encourage for conducting further in vivo investigations to evaluate their potency.

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Cytotoxicity of the Most Active Fraction of the Seeds of Swietenia macrophylla using Human Breast Cancer MCF-7 Cells

Jurnal Kimia Sains dan Aplikasi ◽

10.14710/jksa.23.7.234-237 ◽

2020 ◽

Vol 23 (7) ◽

pp. 234-237

Author(s):

Dudi Tohir ◽

Fitriah Sari

Keyword(s):

Breast Cancer ◽

Ethyl Acetate ◽

Human Breast Cancer ◽

Ethyl Acetate Extract ◽

Artemia Salina ◽

Active Fraction ◽

Human Breast ◽

Swietenia Macrophylla ◽

Brine Shrimp Lethality Assay ◽

Mcf 7

Ethyl acetate fraction from Swietenia macrophylla was reported to have toxicity against the larvae of Artemia salina shrimp larvae. However, there areno reports aboutS. macrophylla, which can inhibit human breast cancer cells MCF-7. Therefore, this study aims to evaluate S. macrophyllaextract's cytotoxicity using human breast cancer MCF-7 cells assay, followed by confirmation of its toxicity using brine shrimp lethality assay. The most active fraction obtained from the ethyl acetate extract of S. macrophylla showed 76.49% inhibition at 50 µg/mL (IC50=34.11 µg/mL). At the same time, the most active fraction may contain a mixture of limonoid compounds after LCMS analysis. The most active fraction obtained from ethyl acetate extract of S. macrophylla showed 76.49%

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