Morphomolecular identification, metabolic profile, anticancer, and antioxidant capacities of Penicillium sp. NRC F1 and Penicillium sp. NRC F16 isolated from Egyptian remote cave

Abstract Searching for remote locations to screen for microorganisms, identify their metabolites, and investigate their bioactivities against lethal diseases such as cancer is of critical importance. In the current study, two fungal strains where isolated from a remote cave in Asyut governorate, Egypt. These isolates were morphologically and molecularly identified through sequencing their ITS region as Penicillium sp. NRC F1, and Penicillium sp. NRC F16. Investigating the metabolic profiles of the silylated ethyl acetate extracts of these fungi through conducting GC-Ms analysis revealed presence of 114 compounds belonging to different chemical classes. On the other hand, studying the in vitro bioactivity of both extracts showed moderate antioxidant activities. Penicillium sp. NRC F1 extract exhibited higher DPPH scavenging activity (74.41 ± 0.59%) at concentration of 200 µg/ml, in comparison with that exerted by Penicillium sp. NRC F16 extract at the same concentration (65.58 ± 1.55%). Moreover, studying the cytotoxicity of extracts against human colon cancer (HCT116), and human breast cancer (MCF7) cell lines revealed that cytotoxicity of both extracts was dose dependent. Promising cytotoxic effect was achieved against human colon cancer HCT116 using 200 µg/ml of Penicillium sp. NRC F1 extract (95.72 ± 1.13 % cytotoxicity), while Penicillium sp. NRC F16 ethyl acetate extract caused a cytotoxicity of 95.43 ± 1.4 %. Similarly, investigating the in vitro cytotoxicity of the extracts against human breast cancer MCF7 cell line resulted in observing promising activity of Penicillium sp. NRC F1 and Penicillium sp. NRC F16 extracts, and they exhibited 97.29 ± 0.61 %; and 97.08 ± 1.07 % cytotoxicity, respectively. Results from this study nominate those strains as promising isolates and encourage for conducting further in vivo investigations to evaluate their potency.

Download Full-text

CYTOTOXIC EFFECTS OF ETHYL ACETATE FRACTIONS FROM SECONDARY METABOLITES OF STREPTOMYCES SP. GMY01 ON HUMAN BREAST CANCER MCF7 CELL LINES

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2017v10s3.21351 ◽

2017 ◽

Vol 10 (14) ◽

pp. 5

Author(s):

Sista Werdyani ◽

Nastiti Wijayanti ◽

Annisa Fitria ◽

Sari Rahmawati

Keyword(s):

Breast Cancer ◽

Secondary Metabolites ◽

Ethyl Acetate ◽

Human Breast Cancer ◽

Cytotoxic Effect ◽

Mcf7 Cell ◽

Ethyl Acetate Extract ◽

Human Breast ◽

Streptomyces Sp ◽

Breast Cancer Mcf7 Cell

Objective: This research aimed to fractionate the ethyl acetate extract from secondary metabolites of Streptomyces sp. GMY01 and to identify which fraction contains cytotoxic active compounds against human breast cancer MCF7 cell lines.Methods: Secondary metabolites were obtained from fermentation of Streptomyces Sp. GMY01 for 15 d. The supernatant containing these secondary metabolites was extracted through partition using ethyl acetate as the solvent. Fractionation of the ethyl acetate extract was conducted via column chromatography using silica gel as the solid phase while the gradient mobile phase consisted of n-hexane, ethyl acetate, and methanol. The cytotoxicity of each fraction was calculated using MTT-assay.Results: The ethyl acetate extract could be separated into 9 fractions using column chromatography. The cytotoxic effect of each fraction differed from each other. The smallest IC50 value was obtained from fraction 4. Further investigation should be conducted to discover the active anticancer compound. The active compound with cytotoxic effect was found in fraction 4 because of the highest IC50 value.Conclusion: This fraction is potential to be investigated more deeply as anticancer, especially for breast cancer.

Download Full-text