scholarly journals THE EFFICACY OF 200 ΜG/KGBW/IP HEAT SHOCK PROTEIN-70 IN REDUCTION OF CYT C, BAX, AND CASPASE 3 EXPRESSION, AND MORTALITY IN MICE MODEL WITH MULTIPLE ORGAN DYSFUNCTION SYNDROME

2018 ◽  
Vol 11 (11) ◽  
pp. 482
Author(s):  
Igl Sukamto ◽  
Suroto Suroto ◽  
Ambar Mudigdo ◽  
Bambang Purwanto
Keyword(s):  
Survival Rate ◽  
Organ Dysfunction ◽  
Multiple Organ Dysfunction Syndrome ◽  
Heat Shock Protein 70 ◽  
Caspase 3 ◽  
Multiple Organ ◽  
Multiple Organ Dysfunction ◽  
Mice Model ◽  
Lung Epithelial ◽  
Cyt C

Objective: Heat shock protein 70 (HSP70) decreases Cyt expression c, Bax, and Caspase 3 in apoptosis multiple organ dysfunction syndrome (MODS), thus inhibiting death. This study aimed to analyze the efficacy of HSP70 200 μg/KgBB/ip to decrease Cyt c, Bax, and Caspase 3 expression, to reduce mortality, and to increase survival rate, in the MOD alveolar lung epithelial of 78-h sepsis model.Methods: This was a post-test only quasi-experiment conducted at Inter-University Central Laboratory of Gadjah Mada University, Yogyakarta, and the Anatomy Pathology Laboratory, Faculty of Medicine, Universitas Sebelas Maret, Surakarta. The study used a type of Balb/c mice, male, aged of 6–8 weeks, body weight of 25–33 g. Sepsis induction used LIG SIGMA L2880-10MG Lot #025M4040V from Escherichia coli 055:B5 purified by phenol extraction. Medication to reduce mortality used HSP70 Lot #L16020515 and then continued with 400× immunohistochemistry (IHC) examination. A sample of 30 mice were divided into three groups: (1) Control group without 78 h treatment, (2) lipopolysaccharide (LPS) group with a dose of 0.25 mg/kgBW/ip 78 h, and (3) HSP70 group with a dose of 200 μg/kgBB/ip after LPS injection 0.25 mg/kgBW/ip 78 h. The outcome variables included expression of Cyt, Bax, Caspase 3, and mortality in mice model with multiple organ dysfunction syndrome. The data were analyzed by Kruskal–Wallis and continued by Mann–Whitney U-test. Results: Administration of HSP70 200 mg/KgBW/ip after LPS 0.25 mg/kgBW/ip significantly decreased Cyt c expression (p=0.014), Bax (p=0.004), and Caspase 3 (p=0.015) in 78 h pulmonary alveolar cells, reduced mortality rate, and increased the number of survivors. Expressions of Cyt c, Bax, and Caspase 3 of IHC 400× magnification had a near-normal image change.Conclusion: There is a decrease of Cyt c, Bax, and Caspase 3 expression in the MOD alveolar lung epithelial cells of the 78-h sepsis model mice, a decrease of mortality rate, and an increase of survival rate, and the image of IHC is almost normal.

scholarly journals MORTALITY PREVENTION IN SEPTIC MICE WITH MULTIPLE ORGAN DYSFUNCTION SYNDROME: THE OPTIMAL DOSE OF INTRAPERITONEAL HEAT SHOCK PROTEIN 70

2018 ◽  
Vol 11 (11) ◽  
pp. 183
Author(s):  
Igl Sukamto ◽  
Bambang Purwanto ◽  
Ambar Mudigdo ◽  
Suroto Suroto ◽  
Bhisma Murti ◽  
...  
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Effective Dose ◽  
Organ Dysfunction ◽  
Multiple Organ Dysfunction Syndrome ◽  
Heat Shock Protein 70 ◽  
Multiple Organ ◽  
Multiple Organ Dysfunction ◽  
Mice Model ◽  
Experimental Group

Objective: Heat shock protein 70 (HSP70) reduces the expression of cytochrome C, B-cell lymphoma-associated X, and cysteine-aspartic acid protease 3 (Caspase 3), in apoptosis of multiple organ dysfunction syndrome (MODS), and thereby can prevent death. Previous researchers used 226 mg/kgBW/intraperitoneal (ip) of HSP70. This study aimed to determine the lowest effective dose of HSP70 to prevent death in sepsis mice model with MODS.Methods: This was a randomized control trial conducted at Pusat Antar Universitas (PAU), Gadjah Mada University, Yogyakarta, Indonesia, from April 1, 2017 to April 21, 2017. The study subjects were Balb/c strain mice. The drug used to induce death was lipopolysaccharides (LPS). The drug used to prevent death in this study was Rat HSP70. A sample of 25 mice was randomized into five groups with each consisting of five mice: (1) Control group with NaCl, (2) experimental group receiving LPS injection of 0.25 mg/kgBW/i.p, (3) experimental group receiving the same dose of LPS injection with HSP70 injection of 100 μg/kgBW/ip, (4) experimental group receiving the same dose of LPS injection with HSP70 injection of 200 μg/kgBW/ip, and (5) experimental group receiving the same dose of LPS injection with HSP70 injection of 300 μg/kgBW/ip. Percentage of live mice between groups was compared by Chi-square test.Results: 3 days after intervention, 13 (86.7%) live mice in the experimental group with ≥100 μg/kgBW/i.p HSP70 were >2 (40%) live mice in the experimental group with <100 μg/kgBW/i.p HSP70, with p=0.038. All mice receiving ≥200 μg/kgBW/ip HSP70 in the experimental group were Alive 3 days after intervention.Conclusion: The lowest effective dose of HSP70 to prevent death in sepsis mice model with MODS is 200 μg/kgBW/ip. All mice are alive 3 days after receiving ≥200 μg/kgBW/ip HSP70. 

Protective effect of sulfated polysaccharide from Lentinula edodes on lung and liver of MODS mice

2021 ◽  
Author(s):  
Wenxue Sun ◽  
Yanbo Feng ◽  
Mengyu Zhang ◽  
Xinling Song ◽  
Le Jia
Keyword(s):  
Protective Effect ◽  
Organ Dysfunction ◽  
Multiple Organ Dysfunction Syndrome ◽  
Lentinula Edodes ◽  
Sulfated Polysaccharide ◽  
Multiple Organ ◽  
Multiple Organ Dysfunction ◽  
Mice Model

In this work, the effects of sulfated polysaccharide from Lentinula edodes (SPLE) on zymosan (ZYM)-induced multiple organ dysfunction syndrome (MODS) mice were investigated. Using MODS mice model, biochemical works have...

Multiple Organ Dysfunction Syndrome of Alcoholic Origin in an 18-Year-Old Patient

2018 ◽  
Vol 2 (12) ◽  
Author(s):  
Francesco Gazia ◽  
Giacomo De Luca ◽  
Imbalzano Gabriele ◽  
Vincenzo Pellicanò
Keyword(s):  
Organ Dysfunction ◽  
Multiple Organ Dysfunction Syndrome ◽  
Multiple Organ ◽  
Multiple Organ Dysfunction

IMPACT probability of poor outcome and plasma cytokine concentrations are associated with multiple organ dysfunction syndrome following traumatic brain injury

2019 ◽  
Vol 131 (6) ◽  
pp. 1931-1937 ◽  
Author(s):  
Sungho Lee ◽  
Hyunsoo Hwang ◽  
Jose-Miguel Yamal ◽  
J. Clay Goodman ◽  
Imoigele P. Aisiku ◽  
...  
Keyword(s):  
Traumatic Brain Injury ◽  
Brain Injury ◽  
Organ Dysfunction ◽  
Multiple Organ Dysfunction Syndrome ◽  
Sofa Score ◽  
Plasma Concentrations ◽  
Multiple Organ ◽  
Multiple Organ Dysfunction ◽  
Poor Outcome ◽  
Initial Plasma

OBJECTIVETraumatic brain injury (TBI) is a major cause of morbidity and mortality. Multiple organ dysfunction syndrome (MODS) occurs frequently after TBI and independently worsens outcome. The present study aimed to identify potential admission characteristics associated with post-TBI MODS.METHODSThe authors performed a secondary analysis of a recent randomized clinical trial studying the effects of erythropoietin and blood transfusion threshold on neurological recovery after TBI. Admission clinical, demographic, laboratory, and imaging parameters were used in a multivariable Cox regression analysis to identify independent risk factors for MODS following TBI, defined as maximum total Sequential Organ Failure Assessment (SOFA) score > 7 within 10 days of TBI.RESULTSTwo hundred patients were initially recruited and 166 were included in the final analysis. Respiratory dysfunction was the most common nonneurological organ system dysfunction, occurring in 62% of the patients. International Mission for Prognosis and Analysis of Clinical Trials (IMPACT) probability of poor outcome at admission was significantly associated with MODS following TBI (odds ratio [OR] 8.88, 95% confidence interval [CI] 1.94–42.68, p < 0.05). However, more commonly used measures of TBI severity, such as the Glasgow Coma Scale, Injury Severity Scale, and Marshall classification, were not associated with post-TBI MODS. In addition, initial plasma concentrations of interleukin (IL)–6, IL-8, and IL-10 were significantly associated with the development of MODS (OR 1.47, 95% CI 1.20–1.80, p < 0.001 for IL-6; OR 1.26, 95% CI 1.01–1.58, p = 0.042 for IL-8; OR 1.77, 95% CI 1.24–2.53, p = 0.002 for IL-10) as well as individual organ dysfunction (SOFA component score ≥ 1). Finally, MODS following TBI was significantly associated with mortality (OR 5.95, 95% CI 2.18–19.14, p = 0.001), and SOFA score was significantly associated with poor outcome at 6 months (Glasgow Outcome Scale score < 4) when analyzed as a continuous variable (OR 1.21, 95% CI 1.06–1.40, p = 0.006).CONCLUSIONSAdmission IMPACT probability of poor outcome and initial plasma concentrations of IL-6, IL-8, and IL-10 were associated with MODS following TBI.

Sign in / Sign up

Export Citation Format

Share Document