UV SPECTROPHOTOMETRIC ANALYSIS AND VALIDATION OF ACYCLOVIR IN SOLID DOSAGE FORM

Objective: A new, economical, sensitive, simple, rapid UV spectrophotometric method has been developed for the estimation of Acyclovir in pure form and pharmaceutical formulation. Methods: This UV method was developed using distilled water as a solvent. In the present method, the wavelength selected for analysis was 254 nm. UV-Visible double beam spectrophotometer (Systronic 2201) was used to carry out the spectral analysis. The ICH guidelines were used to validate the method. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was found in the range of 5-30µg/ml. Accuracy was performed by using a recovery study. The amount of drug recovered was found to be in the range of 100.1-100.5 %. The % RSD value was found to be less than 2. Conclusion: The proposed UV spectroscopic method was found to be accurate, precise, stable, linear, specific, and simple for quantitative estimation of acyclovir in bulk and pharmaceutical dosage form. Hence the present UV spectroscopic method is suitable for the routine assay of acyclovir in bulk and pharmaceutical formulations.

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UV Spectrophotometric Analysis and Validation of Dapsone in Semisolid Dosage Form

Research Journal of Pharmacy and Technology ◽

10.52711/0974-360x.2021.00872 ◽

2021 ◽

pp. 5007-5009

Author(s):

Gajanand Nangare ◽

Varsha Tegeli ◽

Suyash Ingle ◽

Vinod Matole ◽

Avinash Birajdar ◽

...

Keyword(s):

Dosage Form ◽

Quantitative Estimation ◽

Spectroscopic Method ◽

Spectrophotometric Analysis ◽

Ich Guidelines ◽

Double Beam ◽

Recovery Study ◽

Uv Visible ◽

Routine Assay ◽

Stable Linear

Objectives: A new, economical, sensitive, simple, rapid UV spectrophotometric method has been developed for the estimation of Dapsone in pure form and Semisolid Dosage form. Method: This UV method was developed using methanol: RO water (75:25) as a solvent. In the present method the wavelength selected for analysis was 260nm. UV-Visible double beam spectrophotometer (Systronic 2201) was used to carry out spectral analysis. The ICH guidelines were used to validate the method. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was found in the range of 3-18µg/ml. Accuracy was performed by using recovery study. The amount of drug recovered was found to be in the range of 100.1-100.5 %. The %RSD value was found to be less than 2. Conclusion: The proposed UV spectroscopic method was found to be accurate, precise, stable, linear, specific, and simple for quantitative estimation of Dapsone in bulk and Semisolid dosage form. Hence the present UV spectroscopic method is suitable for routine assay of Dapsone in bulk and Semisolid dosage form.

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Method Development and Validation of Evening Primrose oil and its Pharmaceutical dosage form by Simple UV- Spectrophotometric Method

Asian Journal of Pharmaceutical Analysis ◽

10.52711/2231-5675.2021.00023 ◽

2021 ◽

pp. 133-138

Author(s):

Shaheen Begum ◽

Jahnavi. B ◽

Nameera Jabeen ◽

Umema Naaz. T ◽

Sandhya. M

Keyword(s):

Dosage Form ◽

Method Development ◽

Quantitative Estimation ◽

Spectroscopic Method ◽

Evening Primrose Oil ◽

Pharmaceutical Dosage Form ◽

Evening Primrose ◽

Ich Guidelines ◽

Method Development And Validation ◽

Development And Validation

In the present work, simple and sensitive UV spectroscopic method has been developed for the quantitative estimation of Evening Primrose Oil in efem capsule. Cyclohexane was used as solvent. In the range of 100-500μg/ml, the linearity of Evening Primrose Oil shows a correlation co-efficient of 0.999. The UV detection wavelength was 233nm. The percentage RSD for precision and accuracy of the method was found to be less than 2%(LOQ) of 23μg/ml and (LOD) of 7.5μg/ml. The method was validated as per the ICH guidelines. The method was successfully applied for routine analysis of Evening Primrose Oil and in formulation dosage form.

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SPECTROPHOTOMETRIC METHOD FOR THE DETERMINATION AMIKACIN IN PURE AND PHARMACEUTICAL DOSAGE FORM

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2018v10i1.24703 ◽

2018 ◽

Vol 10 (1) ◽

pp. 38

Author(s):

Surya Teja G. ◽

Gurupadayya B. M. ◽

Venkata Sairam K.

Keyword(s):

Dosage Form ◽

Regression Coefficient ◽

Spectroscopic Method ◽

Chloranilic Acid ◽

Pharmaceutical Dosage Form ◽

Ich Guidelines ◽

Highly Sensitive ◽

Acid Reagent ◽

Chromophoric Group ◽

Charge Transfer Complexation

Objective: The aim of the study was to develop an easy, sensible and rapid method for the estimation of amikacin in both pure and marketed formulation using the spectrophotometric method.Methods: Due to lack of chromophoric group in the amikacin, it was derivatized with 0.1 mmol chloranillic acid reagent. For the estimation of amikacin, Shimadzu UV-1700 model spectrophotometer with UV probe software was used. The method was based simple on charge transfer complexation of the drug with a p-chloranilic acid reagent to give a purple coloured product which was measured at 524 nm against blank solution.Results: The derivatised product of amikacin was detected at a wavelength of 524 nm. Linearity was observed with the concentration range of 20-100 µg/ml with a regression coefficient of 0.9803. Results of all the parameters were within the acceptance criteria with % RSD less than 2.Conclusion: The spectroscopic method was validated as per ICH guidelines and was found to be applicable for routine quantitative analysis of amikacin in marketed formulations also. The results of linearity, precision, accuracy LOD and LOQ were within the specified limits. The method is highly sensitive, robust, reproducible and specific.

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Development DEVELOPMENT AND VALIDATION OF NOVEL ULTRAVIOLET SPECTROPHOTOMETRIC METHOD FOR QUANTITATIVE ESTIMATION OF DALFAMPRIDINE IN BULK AND IN PHARMACEUTICAL FORMULATION

Asian Journal of Pharmaceutical and Clinical Research ◽

10.22159/ajpcr.2019.v12i7.33938 ◽

2019 ◽

pp. 275-279

Author(s):

VAIBHAV S KHODKE ◽

GAME MD

Keyword(s):

Spectrophotometric Method ◽

Dosage Form ◽

Quantitative Estimation ◽

Spectroscopic Method ◽

Quality Criteria ◽

Pharmaceutical Dosage Form ◽

Development And Validation ◽

Tablet Dosage ◽

Good Agreement

Objective: The objective of the present study is to develop ultraviolet (UV)-spectroscopic method using pure drug and tablet dosage form that consistently produces a drug with a minimal variation that adheres to quality criteria of purity, identity, and potency. Methods: UV-spectrophotometric method has been developed using a solvent composed of methanol:water (30:70) as a diluent to determine the dalfampridine (DFP) content in bulk and pharmaceutical dosage form at predetermined λmax of 262 nm. Results: It was proved linear in the range of 02–12 μg/ml and exhibited a good correlation coefficient (r2 = 0.9915) and excellent mean recovery (0.004136347%). This method was successfully applied to the determination of DFP content of marketed tablet Dalstep 10 mg (Sun Pharmaceutical Pvt. Ltd.,) from India; the results were in good agreement with the label claims. Conclusion: The method proved to be simple, accurate, precise, specific, robust, and less time consuming and can be applied for the determination of DFP in bulk and marketed formulation.

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Spectrophotometric analysis of Azithromycin and its pharmaceutical dosage forms: Comparison between Spectrophotometry and HPLC

Dhaka University Journal of Pharmaceutical Sciences ◽

10.3329/dujps.v12i2.21981 ◽

2015 ◽

Vol 12 (2) ◽

pp. 171-179 ◽

Cited By ~ 3

Author(s):

Nahid Sharmin ◽

Nazia Sultana Shanta ◽

Sitesh C Bachar

Keyword(s):

Statistical Analysis ◽

Spectrophotometric Method ◽

Dosage Form ◽

Dosage Forms ◽

Spectrophotometric Analysis ◽

Pharmaceutical Dosage Form ◽

Pharmaceutical Dosage Forms ◽

Ich Guidelines ◽

Good Accordance

A simple, reliable, precise and sensitive UV-spectrophotometric method was developed and validated for the estimation of azithromycin in pharmaceutical dosage form and compared with official USP 2010 method. The proposed method utilizes the oxidation of azithromycin with potassium permanganate to liberate formaldehyde. This formaldehyde reacts with acetone-ammonium reagent and produces yellow colored chromogen 3,5-diacetyl-2,6-dihydrolutidine. The colored solution exhibited a maximum absorption at 412 nm which can be detected with UVspectrophotometer. The method was found linear over the concentration range 80% to 120% of the working concentration (R2=0.999). The intra- and inter-day RSD (n = 6) was ? 2.0%. The developed method was validated according to ICH guidelines and values of accuracy, precision and other statistical analysis were found to be in good accordance with the prescribed values. The proposed method was successfully applied for determination of azithromycin and the results have been compared with HPLC and thus enabling the utility of this new method for routine analysis azithromycin in pharmaceutical dosage forms DOI: http://dx.doi.org/10.3329/dujps.v12i2.21981 Dhaka Univ. J. Pharm. Sci. 12(2): 171-179, 2013 (December)

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Development and validation of new analytical method for the simultaneous estimation of amitriptyline and perphenazine in bulk and pharmaceutical dosage form by RP-HPLC

10.33974/ijrpst/ ◽

2018 ◽

Vol 1 (1) ◽

pp. 12-21

Keyword(s):

Flow Rate ◽

Dosage Form ◽

Pharmaceutical Formulations ◽

Hplc Method ◽

Simultaneous Estimation ◽

Pharmaceutical Dosage Form ◽

Rp Hplc ◽

Ich Guidelines ◽

Development And Validation ◽

Isocratic Mode

A new, simple, precise, accurate and reproducible RP-HPLC method for simultaneous estimation of Amitriptyline and Perphenazine in bulk and pharmaceutical formulations was developed. Separation of Amitriptyline and Perphenazine was successfully achieved on Inertsil ODS (250x4.6mm) 5µm column in an isocratic mode utilizing Methanol: ACN: Water (50:30:20) at a flow rate of 1.0 ml/min and eluents were monitored at 253nm with a retention time of 2.440 and 5.503 minutes for Amitriptyline and Perphenazine respectively. The method was validated and it was found to be linear. The values of the correlation coefficient were found to 0.992 for Amitriptyline and 0.9992 for Perphenazine respectively. The LOD for Perphenazine and Amitriptyline were found to be and 33.8µg/ml and 4.2 µg/ml. The LOQ for Perphenazine and Amitriptyline were found to be 20.88µg/ml and 12.12µg/ml respectively. The percentage recoveries for Amitriptyline and Perphenazine were found to be within the limit indicates that the proposed method is highly accurate. The method was extensively validated according to ICH guidelines.

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Analytical Method Development and Validation for the Simultaneous Estimation of Aspirin, Clopidogrel and Rosuvastatin in Pharmaceutical Dosage Form

Journal of Drug Delivery and Therapeutics ◽

10.22270/jddt.v9i4-s.3351 ◽

2019 ◽

Vol 9 (4-s) ◽

pp. 432-438

Author(s):

Pradip Kumar Tiwari ◽

Amit Jain ◽

BK Dubey ◽

GK Pandey ◽

Suresh Dhakad

Keyword(s):

High Performance ◽

Dosage Form ◽

Method Development ◽

Quantitative Estimation ◽

Hplc Method ◽

Simultaneous Estimation ◽

Pharmaceutical Dosage Form ◽

Rp Hplc ◽

Ich Guidelines ◽

Relative Standard

A new, simple, novel, accurate, precise, reliable, rapid and linear reverse phase high-performance liquid chromatography (RP-HPLC) method was developed and fully validated for simultaneous qualitative and quantitative estimation of Rosuvastatin (ROS), Clopidogrel (CLOP) and Aspirin (ASP) in bulk and pharmaceutical dosage form as per International Conference on Harmonization (ICH) guidelines. In the present work, good chromatographic separation was achieved by isocratic method using a Hypersil BDS C18 column (250 mm ×4.6, 5 μm) and a mobile phase consisting of KH2Po4 buffer pH-6.0: acetonitrile in the ratio 60:40, at a flow rate of 1 ml/min. The effluents obtained were monitored at 242nm with the UV-visible detector. The calibration curves obtained were linear (r2=0.999) over the concentration range of 7.5-22.5μg/ml and 1-3μg/ml for CLOP, ASP and ROS respectively. A run time of 7.0 minutes for each sample made it possible to analyze more than 200 samples per day. The retention time of ASP, CLOP and ROS was found to be 3.103 min, 4,277 min and 5.707 min respectively. The high recovery values (99%-101%) indicate a satisfactory accuracy. The low percent relative standard deviation (% RSD) values in the precision study reveal that the method is precise therefore the method can be used for routine monitoring of CLOP, ASP and ROS in industry in the assay of bulk drug and dosage form. Keywords: RP-HPLC, Rosuvastatin, Clopidogrel, Aspirin, Method validation, ICH guidelines.

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Development and validation of new analytical method for the simultaneous estimation of amitriptyline and perphenazine in bulk and pharmaceutical dosage form by RP-HPLC

International Journal of Research in Pharmaceutical Sciences and Technology ◽

10.33974/ijrpst.v1i1.19 ◽

2018 ◽

Vol 1 (1) ◽

pp. 12-21

Author(s):

V Pavan Kumar ◽

B Ramadevi ◽

Shaik Karishma ◽

P Divya ◽

B Sivagami ◽

...

Keyword(s):

Flow Rate ◽

Dosage Form ◽

Pharmaceutical Formulations ◽

Hplc Method ◽

Simultaneous Estimation ◽

Pharmaceutical Dosage Form ◽

Rp Hplc ◽

Ich Guidelines ◽

Development And Validation ◽

Isocratic Mode

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Colorimetric Quantitative Estimation of Abacavir Sulphate in Pharmaceutical Dosage Form

Asian journal of biochemical and pharmaceutical research ◽

10.24214/ajbpr/8/1/4749 ◽

2018 ◽

Vol 8 (1) ◽

Keyword(s):

Dosage Form ◽

Quantitative Estimation ◽

Pharmaceutical Dosage Form

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UV SPECTROPHOTOMETRIC ANALYSIS AND VALIDATION OF OSELTAMIVIRPHOSPHATE IN PURE AND PHARMACEUTICAL FORMULATION

International Journal of Current Pharmaceutical Research ◽

10.22159/ijcpr.2020v12i2.37505 ◽

2020 ◽

pp. 111-114 ◽

Cited By ~ 1

Author(s):

SACHIN A. YANJANE ◽

SHRISHAIL M. GHURGHURE ◽

VINOD K. MATOLE

Keyword(s):

Spectrophotometric Method ◽

Spectrophotometric Analysis ◽

Pharmaceutical Formulation ◽

Ich Guidelines ◽

Highly Sensitive ◽

Double Beam ◽

Recovery Study ◽

Uv Visible ◽

Oseltamivir Phosphate ◽

Simple Economic

Objective: A new, simple, economical, precise, sensitive, linear, accurate, rapid UV spectrophotometric method has been developed for the estimation of Oseltamivir Phosphate in pure form and pharmaceutical formulation. Methods: This UV method was developed using Methanol as a solvent. In the present method, the wavelength selected for analysis was 218 nm. UV-Visible double beam spectrophotometer (Systronic 2201) was used to carry out spectral analysis. The ICH guidelines were used to validate the method. Results: The method was validated for linearity, range, accuracy, precision, robustness, LOD and LOQ. Linearity was found in the range of 10-50µg/ml. Accuracy was performed by using a recovery study. The amount of drug recovered was found to be in the range of 99.01-100.1%. The % RSD value was found to be less than 2. Conclusion: The developed UV spectrophotometric method was found to be simple, economic, sensitive, easy, accurate, linear, specific and highly sensitive and can be used for routine estimation of Oseltamivir Phosphate.

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