The polyphasic analysis of two native Raphidiopsis isolates supports the unification of the genera Raphidiopsis and Cylindrospermopsis (Nostocales, Cyanobacteria)

Four strains isolated in the last 15 years were revealed to be identical in their 16S rRNA gene sequences to MCRO19, the sequence of which was deposited in GenBank in 1995. In a polyphasic analysis including phenotypic and genotypic features, the five strains (including MCRO19), which had been isolated in four European countries, turned out to represent a unique taxonomic entity. They are scotochromogenic slow growers and are genetically related to the group that included Mycobacterium simiae and 15 other species. The novel species Mycobacterium europaeum sp. nov. is proposed to accommodate these five strains. Strain FI-95228T ( = DSM 45397T = CCUG 58464T) was chosen as the type strain. In addition, a thorough revision of the phenotypic and genotypic characters of the species related to M. simiae was conducted which leads us to suggest the denomination of the ‘Mycobacterium simiae complex’ for this group.

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Polyphasic analysis in the description of Sulfitobacter salinus sp. nov., a marine alphaproteobacterium isolated from seawater

Annals of Microbiology ◽

10.1007/s13213-019-01515-1 ◽

2019 ◽

Vol 69 (12) ◽

pp. 1301-1308 ◽

Cited By ~ 5

Author(s):

Jaewoo Yoon

Keyword(s):

Polyphasic Analysis

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Brachybacterium zhongshanense sp. nov., a cellulose-decomposing bacterium from sediment along the Qijiang River, Zhongshan City, China

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.64968-0 ◽

2007 ◽

Vol 57 (11) ◽

pp. 2519-2524 ◽

Cited By ~ 24

Author(s):

Guoxia Zhang ◽

Guoqu Zeng ◽

Xiaowei Cai ◽

Suier Deng ◽

Huidong Luo ◽

...

Keyword(s):

Dna Hybridization ◽

Insertion Sequence ◽

Gene Sequence ◽

Rrna Gene ◽

Ratio Analysis ◽

Polyphasic Analysis ◽

Hybridization Data ◽

Dna Base ◽

Gene Sequence Analysis ◽

Bacterium Strain

A cellulose-decomposing bacterium, strain JBT, was isolated from sediments along the Qijiang River, Zhongshan City, China. Results of morphological, biochemical and chemotaxonomic characterization and 16S rRNA gene sequence analysis revealed that strain JBT belonged to the genus Brachybacterium. Insertion sequence-PCR fingerprinting patterns, DNA base ratio analysis and DNA–DNA hybridization data showed that strain JBT differed from recognized species of the genus Brachybacterium. Based on polyphasic analysis, strain JBT represents a novel species of the genus Brachybacterium, for which the name Brachybacterium zhongshanense sp. nov. is proposed. The type strain is JBT (=LMG 23926T=CGMCC 1.6508T=DSM 18832T).

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Polyphasic Analysis of Intraspecific Diversity in Epicoccum nigrum Warrants Reclassification into Separate Species

PLoS ONE ◽

10.1371/journal.pone.0014828 ◽

2011 ◽

Vol 6 (8) ◽

pp. e14828 ◽

Cited By ~ 29

Author(s):

Léia Cecilia de Lima Fávaro ◽

Fernando Lucas de Melo ◽

Carlos Ivan Aguilar-Vildoso ◽

Welington Luiz Araújo

Keyword(s):

Intraspecific Diversity ◽

Polyphasic Analysis ◽

Separate Species ◽

Epicoccum Nigrum

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Polyphasic analysis reveals correlation between phenotypic and genotypic analysis in soybean bradyrhizobia (Bradyrhizobium spp.)

Systematic and Applied Microbiology ◽

10.1016/j.syapm.2020.126073 ◽

2020 ◽

Vol 43 (3) ◽

pp. 126073

Author(s):

P. Joglekar ◽

C.P. Mesa ◽

V.A. Richards ◽

S.W. Polson ◽

K.E. Wommack ◽

...

Keyword(s):

Polyphasic Analysis ◽

Genotypic Analysis ◽

Bradyrhizobium Spp

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Polyphasic Analysis of a Middle Ages Coprolite Microbiota, Belgium

PLoS ONE ◽

10.1371/journal.pone.0088376 ◽

2014 ◽

Vol 9 (2) ◽

pp. e88376 ◽

Cited By ~ 28

Author(s):

Sandra Appelt ◽

Fabrice Armougom ◽

Matthieu Le Bailly ◽

Catherine Robert ◽

Michel Drancourt

Keyword(s):

Middle Ages ◽

Polyphasic Analysis

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Polyphasic analysis of Thermus isolates from geothermal areas in Iceland

Extremophiles ◽

10.1007/s00792-006-0530-3 ◽

2006 ◽

Vol 10 (6) ◽

pp. 563-575 ◽

Cited By ~ 11

Author(s):

Gudmundur O. Hreggvidsson ◽

Sigurlaug Skirnisdottir ◽

Bart Smit ◽

Sigridur Hjorleifsdottir ◽

Viggo Th. Marteinsson ◽

...

Keyword(s):

Polyphasic Analysis

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Permianibacter aggregans gen. nov., sp. nov., a bacterium of the family Pseudomonadaceae capable of aggregating potential biofuel-producing microalgae

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY ◽

10.1099/ijs.0.065003-0 ◽

2014 ◽

Vol 64 (Pt_10) ◽

pp. 3503-3507 ◽

Cited By ~ 8

Author(s):

Hui Wang ◽

Tianling Zheng ◽

Russell T. Hill ◽

Xiaoke Hu

Keyword(s):

Bacterial Strain ◽

Algal Culture ◽

Rrna Gene ◽

The Novel ◽

Polyphasic Analysis ◽

Respiratory Quinone ◽

Polar Lipid Profile ◽

Content Type ◽

Link Type ◽

The Family

A novel bacterial strain, capable of aggregating potential biofuel-producing microalgae, was isolated from the phycosphere of an algal culture and designated HW001T. The novel bacterial strain was identified on the basis of its phylogenetic, genotypic, chemotaxonomic and phenotypic characteristics in this study. Cells were aerobic, Gram-negative rods. 16S rRNA gene-based phylogenetic analysis revealed that strain HW001T is affiliated with the family Pseudomonadaceae in the phylum Proteobacteria , but forms a distinct clade within this family. The DNA G+C content of strain HW001T was 55.4 mol%. The predominant cellular fatty acids were iso-C15 : 0, summed feature 9 (iso-C17 : 1ω9c), C16 : 0 and summed feature 3 (C16 : 1ω7c/C16 : 1ω6c). Q-8 was the main respiratory quinone. The polar lipid profile contained phosphatidylethanolamine, an unidentified aminophospholipid and some unidentified lipids. Based on the extensive polyphasic analysis, strain HW001T represents a novel species of a new genus in the family Pseudomonadaceae , for which the name Permianibacter aggregans gen. nov., sp. nov., is proposed. The type strain of the type species is HW001T ( = CICC 10856T = KCTC 32485T).

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