Triglyceride-rich lipoproteins and endothelial dysfunction: molecular mechanisms and gene expression studies

2007 ◽  
Vol 2 (2) ◽  
pp. 119-122
Author(s):  
Giuseppe Danilo Norata ◽  
Alberico Luigi Catapano
Keyword(s):  
Gene Expression ◽  
Endothelial Dysfunction ◽  
Molecular Mechanisms ◽  
Expression Studies ◽  
Gene Expression Studies

Post-prandial endothelial dysfunction in hypertriglyceridemic subjects: Molecular mechanisms and gene expression studies

2007 ◽  
Vol 193 (2) ◽  
pp. 321-327 ◽  
Author(s):  
Giuseppe Danilo Norata ◽  
Liliana Grigore ◽  
Sara Raselli ◽  
Laura Redaelli ◽  
Anders Hamsten ◽  
...  
Keyword(s):  
Gene Expression ◽  
Endothelial Dysfunction ◽  
Molecular Mechanisms ◽  
Expression Studies ◽  
Gene Expression Studies

scholarly journals Trisomy 21 and Down syndrome: a short review

2008 ◽  
Vol 68 (2) ◽  
pp. 447-452 ◽  
Author(s):  
CA. Sommer ◽  
F. Henrique-Silva
Keyword(s):  
Gene Expression ◽  
Down Syndrome ◽  
Large Scale ◽  
Molecular Mechanisms ◽  
Global Gene Expression ◽  
Short Review ◽  
Complex Disorder ◽  
Expression Studies ◽  
Gene Expression Studies ◽  
Chromosome Segments

Even though the molecular mechanisms underlying the Down syndrome (DS) phenotypes remain obscure, the characterization of the genes and conserved non-genic sequences of HSA21 together with large-scale gene expression studies in DS tissues are enhancing our understanding of this complex disorder. Also, mouse models of DS provide invaluable tools to correlate genes or chromosome segments to specific phenotypes. Here we discuss the possible contribution of HSA21 genes to DS and data from global gene expression studies of trisomic samples.

Recent advances in psychoneuroimmunology: Inflammation in psychiatric disorders

2011 ◽  
Vol 2 (2) ◽  
Author(s):  
Monojit Debnath ◽  
Karen Doyle ◽  
Camilla Langan ◽  
Colm McDonald ◽  
Brian Leonard ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bipolar Disorder ◽  
Psychiatric Disorders ◽  
Molecular Mechanisms ◽  
Molecular Genetic ◽  
Major Depressive ◽  
Immune System Dysfunction ◽  
Expression Studies ◽  
Gene Expression Studies

AbstractPsychiatric disorders are common and complex and their precise biological underpinnings remain elusive. Multiple epidemiological, molecular, genetic and gene expression studies suggest that immune system dysfunction may contribute to the risk for developing psychiatric disorders including schizophrenia, bipolar disorder, and major depressive disorder. However, the precise mechanisms by which inflammation-related events confer such risk are unclear. In this review, we examine the peripheral and central evidence for inflammation in psychiatric disorders and the potential molecular mechanisms implicated including inhibition of neurogenesis, apoptosis, the HPA-axis, the role of brain-derived neurotrophic factor and the interplay between the glutamatergic, dopaminergic and serotonergic neurotransmitter systems.

scholarly journals Identification of Reference Genes in Human Myelomonocytic Cells for Gene Expression Studies in Altered Gravity

2015 ◽  
Vol 2015 ◽  
pp. 1-20 ◽  
Author(s):  
Cora S. Thiel ◽  
Swantje Hauschild ◽  
Svantje Tauber ◽  
Katrin Paulsen ◽  
Christiane Raig ◽  
...  
Keyword(s):  
Gene Expression ◽  
Reference Genes ◽  
Molecular Mechanisms ◽  
Expression Profiles ◽  
Housekeeping Genes ◽  
Altered Gravity ◽  
Experimental Conditions ◽  
Expression Levels ◽  
Expression Studies ◽  
Gene Expression Studies

Gene expression studies are indispensable for investigation and elucidation of molecular mechanisms. For the process of normalization, reference genes (“housekeeping genes”) are essential to verify gene expression analysis. Thus, it is assumed that these reference genes demonstrate similar expression levels over all experimental conditions. However, common recommendations about reference genes were established during 1 g conditions and therefore their applicability in studies with altered gravity has not been demonstrated yet. The microarray technology is frequently used to generate expression profiles under defined conditions and to determine the relative difference in expression levels between two or more different states. In our study, we searched for potential reference genes with stable expression during different gravitational conditions (microgravity, normogravity, and hypergravity) which are additionally not altered in different hardware systems. We were able to identify eight genes (ALB, B4GALT6, GAPDH, HMBS, YWHAZ, ABCA5, ABCA9, and ABCC1) which demonstrated no altered gene expression levels in all tested conditions and therefore represent good candidates for the standardization of gene expression studies in altered gravity.

scholarly journals Assessment of common housekeeping genes as reference for gene expression studies using RT-qPCR in mouse choroid plexus

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Kim Hoa Ho ◽  
Annarita Patrizi
Keyword(s):  
Gene Expression ◽  
Choroid Plexus ◽  
Sensory Deprivation ◽  
Housekeeping Genes ◽  
Housekeeping Gene ◽  
Experimental Conditions ◽  
Brain Repair ◽  
Expression Studies ◽  
Testing Algorithms ◽  
Gene Expression Studies

AbstractChoroid plexus (ChP), a vascularized secretory epithelium located in all brain ventricles, plays critical roles in development, homeostasis and brain repair. Reverse transcription quantitative real-time PCR (RT-qPCR) is a popular and useful technique for measuring gene expression changes and also widely used in ChP studies. However, the reliability of RT-qPCR data is strongly dependent on the choice of reference genes, which are supposed to be stable across all samples. In this study, we validated the expression of 12 well established housekeeping genes in ChP in 2 independent experimental paradigms by using popular stability testing algorithms: BestKeeper, DeltaCq, geNorm and NormFinder. Rer1 and Rpl13a were identified as the most stable genes throughout mouse ChP development, while Hprt1 and Rpl27 were the most stable genes across conditions in a mouse sensory deprivation experiment. In addition, Rpl13a, Rpl27 and Tbp were mutually among the top five most stable genes in both experiments. Normalisation of Ttr and Otx2 expression levels using different housekeeping gene combinations demonstrated the profound effect of reference gene choice on target gene expression. Our study emphasized the importance of validating and selecting stable housekeeping genes under specific experimental conditions.

Sign in / Sign up

Export Citation Format

Share Document