scholarly journals Endosomal escape enhancing compounds facilitate functional delivery of extracellular vesicle cargo

Nanomedicine ◽  
2019 ◽  
Vol 14 (21) ◽  
pp. 2799-2814 ◽  
Author(s):  
Nikki Heath ◽  
Xabier Osteikoetxea ◽  
Taiana Mia de Oliveria ◽  
Elisa Lázaro-Ibáñez ◽  
Olga Shatnyeva ◽  
...  
Keyword(s):  
Cell Line ◽  
Extracellular Vesicles ◽  
Extracellular Vesicle ◽  
Cre Recombinase ◽  
Endosomal Escape ◽  
Reporter Cell Line ◽  
Reporter Cell ◽  
Delivery Vehicles

Aim: Extracellular vesicles (EVs) are desirable delivery vehicles for therapeutic cargoes. We aimed to load EVs with Cre recombinase protein and determine whether functional delivery to cells could be improved by using endosomal escape enhancing compounds. Materials & methods: Overexpressed CreFRB protein was actively loaded into EVs by rapalog-induced dimerization to CD81FKBP, or passively loaded by overexpression in the absence of rapalog. Functional delivery of CreFRB was analysed using a HEK293 Cre reporter cell line in the absence and presence of endosomal escape enhancing compounds. Results: The EVs loaded with CreFRB by both active and passive mechanisms were able to deliver functional CreFRB to recipient cells only in the presence of endosomal escape enhancing compounds chloroquine and UNC10217832A. Conclusion: The use of endosomal escape enhancing compounds in conjunction with EVs loaded with therapeutic cargoes may improve efficacy of future EV based therapeutics.

Establishment of a Transgenic Pig Fetal Fibroblast Reporter Cell Line for Monitoring Cre Recombinase Activity

2009 ◽  
Vol 28 (6) ◽  
pp. 303-308 ◽  
Author(s):  
Li Li ◽  
Daxin Pang ◽  
Limei Chen ◽  
Tiedong Wang ◽  
Daibang Nie ◽  
...  
Keyword(s):  
Cell Line ◽  
Cre Recombinase ◽  
Reporter Cell Line ◽  
Transgenic Pig ◽  
Reporter Cell ◽  
Fetal Fibroblast

scholarly journals Generation of a NKX2.1 – p63 double transgenic knock-in reporter cell line from human induced pluripotent stem cells (MHHi006-A-4)

2020 ◽  
Vol 42 ◽  
pp. 101659
Author(s):  
Nora Drick ◽  
Anais Sahabian ◽  
Praeploy Pongpamorn ◽  
Sylvia Merkert ◽  
Gudrun Göhring ◽  
...  
Keyword(s):  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Cell Line ◽  
Pluripotent Stem Cells ◽  
Reporter Cell Line ◽  
Reporter Cell ◽  
Induced Pluripotent

scholarly journals Generation of a Jurkat-based fluorescent reporter cell line to evaluate lipid antigen interaction with the human iNKT cell receptor

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Piotr Humeniuk ◽  
Sabine Geiselhart ◽  
Claire Battin ◽  
Tonya Webb ◽  
Peter Steinberger ◽  
...  
Keyword(s):  
Cell Line ◽  
Cell Receptor ◽  
Inkt Cell ◽  
Reporter Cell Line ◽  
Reporter Cell ◽  
Fluorescent Reporter ◽  
Lipid Antigen ◽  
Antigen Interaction

scholarly journals Development and characterization of a novel, megakaryocyte NF‐κB reporter cell line for investigating inflammatory responses

2020 ◽  
Vol 19 (1) ◽  
pp. 107-120
Author(s):  
Thomas M. Vallance ◽  
Jonathan J. Sheard ◽  
Yiming Meng ◽  
Enrico C. Torre ◽  
Ketan Patel ◽  
...  
Keyword(s):  
Cell Line ◽  
Inflammatory Responses ◽  
Reporter Cell Line ◽  
Reporter Cell
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