Guided bone regeneration with a gelatin layer and adenoviral delivery of c-myb enhances bone healing in rat tibia

2020 ◽  
Vol 15 (7) ◽  
pp. 1877-1890
Author(s):  
Jyoti Shrestha Takanche ◽  
Ji-Eun Kim ◽  
Jeong-Seok Kim ◽  
Ho-Keun Yi
Keyword(s):  
Bone Healing ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
New Bone Formation ◽  
Mineral Density ◽  
Bone Injury ◽  
Adenoviral Delivery ◽  
Gelatin Layer ◽  
Rat Tibia

Aim: Bone healing becomes problematic during certain states, such as trauma. This study verifies whether the application of c-myb with gelatin promotes bone healing during bone injuries. Materials & methods: A biodegradable membrane was modified with adenoviral vector c-myb ( Ad/c-myb) and gelatin and applied in the bone injury site of rat tibia. Results: c-myb enhanced osteogenic differentiation and mineralization in bone marrow stromal cells after induction with osteogenic media. In vivo examination of rat tibia after application of the biodegradable membrane with Ad/c-myb and a gelatin layer demonstrated increased bone volume, bone mineral density, new bone formation and osteogenic molecules, compared with Ad/LacZ. Conclusion: c-myb has the potential to assist bone healing and may be applicable to the treatment of bone during injury.

Dimethyloxallyl Glycine-Incorporated Borosilicate Bioactive Glass Scaffolds for Improving Angiogenesis and Osteogenesis in Critical-Sized Calvarial Defects

2019 ◽  
Vol 16 (6) ◽  
pp. 565-576 ◽  
Author(s):  
Xiangyun Jin ◽  
Dan Han ◽  
Jie Tao ◽  
Yinjun Huang ◽  
Zihui Zhou ◽  
...  
Keyword(s):  
Bioactive Glass ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
In Vivo Studies ◽  
New Bone Formation ◽  
Calvarial Bone ◽  
Calvarial Defects

Background: In the field of bone tissue engineering, there has been an increasing interest in biomedical materials with both high angiogenic ability and osteogenic ability. Among various osteogenesis materials, bioactive borosilicate and borate glass scaffolds possess suitable degradation rate and mechanical strength, thus drawing many scholars’ interests and attention. Objective: In this study, we fabricated bioactive glass scaffolds composed of borosilicate 2B6Sr using the Template-Method and incorporated Dimethyloxalylglycine (DMOG), a small-molecule angiogenic drug possessing good angiogenic ability, to improve bone regeneration. Methods: The in-vitro studies showed that porous borosilicate bioactive glass scaffolds released slowly, a steady amount of DMOG and stimulated the proliferation and osteogenic differentiation of human bone marrow stromal cells hBMSCs. Results: In-vivo studies showed that the borosilicate bioactive glass scaffolds could significantly promote new bone formation and neovascularization in rats’ calvarial bone defects. Conclusion: These results indicated that DMOG-incorporated bioactive glass scaffold is a successful compound with excellent angiogenesis-osteogenesis ability, which has favorable clinical prospects.

scholarly journals New Bone Formation in Tuberculous-Infected Vertebral Body Defect after Administration of Bone Marrow Stromal Cells in Rabbit Model

2016 ◽  
Vol 10 (1) ◽  
pp. 1 ◽  
Author(s):  
Ahmad Jabir Rahyussalim ◽  
Tri Kurniawati ◽  
Nurjati Chairani Siregar ◽  
Agus Syahrurachman ◽  
Ismail Hadisubroto Dilogo ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Bone Formation ◽  
Vertebral Body ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Rabbit Model ◽  
Marrow Stromal Cells ◽  
New Bone Formation

Effectiveness of Cryo-Preserved Human Bone Marrow Stromal Cells at Forming Bone in Vivo

2005 ◽  
Vol 116 (Supplement) ◽  
pp. 150-151
Author(s):  
Mahesh H. Mankani ◽  
Sergei Kuznetsov ◽  
Pamela Gehron Robey
Keyword(s):  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow

In Vivo Matrix Production by Bone Marrow Stromal Cells Seeded on PLGA Scaffolds for Ligament Tissue Engineering

2009 ◽  
Vol 15 (10) ◽  
pp. 3109-3117 ◽  
Author(s):  
Floor van Eijk ◽  
Daniel B.F. Saris ◽  
Natalja E. Fedorovich ◽  
Moyo C. Kruyt ◽  
W. Jaap Willems ◽  
...  
Keyword(s):  
Tissue Engineering ◽  
Bone Marrow ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Matrix Production ◽  
Ligament Tissue Engineering
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