ISOLASI BAKTERI PATTOGEN PADA PASIEN PENDERITA INFEKSI TELINGA Chronic supparative otitis media (OMSK)

AbstrakTujuan penelitian ini adalah mengisolasi bakteri pattogen pada infeksi telinga Chronic suppurative otitis media yang nantinya sebagai tahap awal dari pengembangan analisa antimikroba Bakteri Asam Laktat (BAL) pada proses fermentasi santan kelapa menjadi Virgin Coconut Oil (VCO), yang diharapkan mampu berfungsi sebagai antimikroba/antibakteri dari bakteri pattogen. Menurut Suryani dkk (2014), isolat BAL dari fermentasi santan mampu berfungsi sebagai antibakteri terhadap 5 bakteri uji (E.coli NBRC14237, Staphylococcus aereus NBRC 13276, Bacillus substilis BTCCB, Salmonella thypii, dan Listeria monocytogenes ). Untuk mendapatkan informasi tersebut maka di isolasi bakteri yang ada di cairan telinga pasien penderita infeksi telinga Chronic suppurative otitis media dengan menggunakan media umum Blood Agar dengan metoda Pengenceran. Dari penelitian ini didapatkan 42 isolat dengan 4 jenis bakteri pattogen yaitu Pseudomonas aureginosa, Staphilococus aureus, Staphilococus epidermidis, Proteus mirabilis. Pasien OMSK yang diambil sekret nya 60 % berumur diatas 20 tahun dan 40% berumur dibawah 20 tahun yang sama banyak antara perempuan dan Laki-laki . Kata Kunci: Isolasi bakteri pattogen sekret pasien OMSK, Chronic suppurative otitis media,BAL( Bakteri Asam Laktat), Virgin Coconut Oil (VCO), The Isolation of Pathogen Bacteria on The Sufferer of Ear Infection Chronic Supparative Otitis Media1Suryani, 1Zulmardi,2 Abdi Dharma, 2Yunazar Manjang1Universitas Muhammadiyah Sumatera Barat2Universitas AndalasE-mail [email protected] AbsractThis research is aimed to isolate pathogen bacteria in ear infection Chronic Supparative Otitis Media. This is the first phase in developing analysis Antimicroba Lactic Acid. Bacteria in the process of coconut milk fermentation into Virgin Coconut Oil (VCO). It is expected can be fanctioned as antibacteria of pathogen. According to Suryani et.al (2014), isolate of Antimicroba Lactic Acid of coconut milk fermentation can act as antibacteria against 5 examined bacteria (E.coli NBRC14237, Staphylococcus aereus NBRC 13276, Bacillus substilis BTCCB, Salmonella thypii, dan Listeria monocytogenes). To achieve it, the ear liquid of sufferer containing bacteria is isolated with Blood Agar media and Dilution method. The result is that there 42 isolate in accordance with 3 kinds pathogen bacteria such as Pseudomonas aureginosa, Staphilococus aureus, Staphilococus epidermidis, Proteus mirabilis. The sufferer who has choosen as participant are 72% coming from the age of above 20 and the rest are under 20 years old. The ratio of sexes are similar between male and female.Key Word: Isolation pattogen bacteria sckret OMSK, Chronic suppurative otitis media, LAB(Lactat Acid Bacteria), Virgin Coconut Oil (VCO),

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Microbiology and antibiotic sensitivity of uncomplicated chronic suppurative otitis media at Dr. George Mukhari Academic Hospital, South Africa

International Journal of Otorhinolaryngology and Head and Neck Surgery ◽

10.18203/issn.2454-5929.ijohns20211177 ◽

2021 ◽

Vol 7 (4) ◽

pp. 565

Author(s):

Monica Kotu ◽

Ian Paul Olwoch

Keyword(s):

Staphylococcus Aureus ◽

Pseudomonas Aeruginosa ◽

Otitis Media ◽

Proteus Mirabilis ◽

Antibiotic Sensitivity ◽

Chronic Suppurative Otitis Media ◽

Older Children ◽

Suppurative Otitis Media ◽

Sensitivity Pattern ◽

Age Range

Background: The study was conducted to assess the type and frequency of isolation of different microorganisms in uncomplicated chronic suppurative otitis media (CSOM) and their antibiotic sensitivity in our institution.Methods: A total of 88 consecutive patients with unilateral or bilateral active, chronic suppurative otitis media attending outpatient department at DGMAH were included in the study after obtaining an informed consent. There were 55 males (62.5%) and 33 females (37.9%) with age range between 6 months and 76 years. Pus swabs were taken through the perforation site and from the promontory after ear mopping under direct vision. Results: Children less than 5 years were more affected (23.9%) than older children 5-10 years (13.6%) and 11-15 years (11.45%). One hundred and six microorganisms were isolated from analysis of cultures obtained from 72 patients. Seven cultures were negative (5.9%), 8 specimens were contaminated (6.7%) and 1 specimen was lost (1.1%). Pseudomonas aeruginosa (24.0%) was the most common isolate, followed by Staphylococcus aureus (17%) and Proteus mirabilis (10%). Drug sensitivity pattern showed that Piperacillin-tazobactam was effective against the majority of Pseudomonas aeruginosa isolates at 72%, followed by both Gentamicin and Ceftazidime at 64% and Ciprofloxacin at 48%. Staphylococcus aureus isolates were sensitive to Erythromycin (77%), Cloxacillin and Clindamycin at 72%. Proteus mirabilis was sensitive to Cefuroxime (91%), Co-amoxiclav (72.8%).Conclusions: Isolation rate and susceptibility patterns in CSOM, suggest a need for regular surveillance to monitor antimicrobial resistance and to guide antibacterial therapy. <h1> </h1>

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Perbedaan sensitivitas tetes telinga antibiotik terhadap Pseudomonas aeruginosa pada otitis media supuratif kronik

Oto Rhino Laryngologica Indonesiana ◽

10.32637/orli.v42i2.22 ◽

2012 ◽

Vol 42 (2) ◽

Author(s):

Anton Budhi Darmawan ◽

Dwi Utami Anjarwati

Keyword(s):

Pseudomonas Aeruginosa ◽

Otitis Media ◽

Chronic Suppurative Otitis Media ◽

Test Results ◽

Sectional Study ◽

Suppurative Otitis Media ◽

Number Of Patients ◽

Significant Difference ◽

Cochran Test ◽

Post Hoc

Background: Chronic suppurative otitis media (CSOM) is one infectious disease of the middle ear, most commonly caused by Pseudomonas aeruginosa. A high number of patients come to the ENT outpatient clinic with active benign type of CSOM. The bacteria Pseudomonas aeruginosa is capable of producing biofilm which protects itself from penetration of antibiotics, and therefore creates resistance towards antibiotics and difficult to eradicate. Objective: The aim of this study was to compare the sensitivity levels of chloramphenicol, polymyxin-neomycin, cyprofloxacin and ofloxacine against Pseudomonas aeruginosa in patients with active benign type CSOM in ENT clinic. Method: The method used was across sectional study on 25 patients, from August 2010 until December 2010. Samples were taken withear swab and then put on sensitivity test to chloramphenicol, polymyxin-neomycin, cyprofloxacin andofloxacine using the diffusion disc method. The analysis used in this study was Cochran test. Results: Results showed a significant difference in sensitivity among chloramphenicol (38,70%), polymyxinneomycin(83,87%),cyprofloxacin(90,32%)andofloxacin(58,06%)withp=0,000(p<0,05).PostHocanalysisusing the Mc Nemar indicated that there were significant differences in sensitivity betweenpolymyxin-neomycin to chloramphenicol with p=0,000 (p<0,05), ciprofloxacin to chloramphenicol andciprofloxacin to ofloxacine with p= 0,002, but there were no significant differences between cyprofloxacinto polymyxin-neomycin with p=0,687, polymyxin-neomycin to ofloxacin p=0.057 and ofloxacin tochloramphenicol p=0,109. There were significant differences in antibiotic ear dropssensitivity to Pseudomonas aeruginosa in patients with active benign type of CSOM. Cyprofloxacin andpolymyxin-neomycin were more sensitive than ofloxacin and chloramphenicol. Keywords: Pseudomonas aeruginosa, active benign type of chronic suppurative otitis media, antibioticear drops. Abstrak : Latar belakang: Otitis media supuratif kronik (OMSK) merupakan penyakit infeksi kronik telinga tengah yang sering dijumpai di klinik THT. Penyebab tersering OMSK adalah bakteri Pseudomonasaeruginosa. Pseudomonas aeruginosa mempunyai kemampuan untuk membentuk biofilm yangmelindunginya dari penetrasi antibiotik sehingga menimbulkan resistensi terhadap antibiotik dan sulituntuk eradikasinya. Tujuan: Untuk mengetahui perbandingan tingkat sensitivitas kloramfenikol,polimiksin-neomisin, ciprofloksasin dan ofloksasin terhadap isolat Pseudomonas aeruginosa padapasien OMSK benigna aktif di klinik THT RSMS. Metode: Metode yang digunakan adalah crosssectional terhadap 29 pasien OMSK di klinik THT RSMS periode bulan Agustus 2010 - Desember2010. Pengambilan sampel dilakukan dengan swab telinga. Uji sensitivitas terhadap kloramfenikol,polimiksin-neomisin, ciprofloksasin dan ofloksasin dilakukan dengan metode cakram secara difusi.Analisis yang digunakan dalam penelitian ini adalah Cochran dan analisis post hoc. Hasil: Didapatkansensitivitas kloramfenikol sebesar 38,70%, polimiksin-neomisin sebesar 83,87%, ciprofloksasin sebesar90,32% dan ofloksasin sebesar 58,06% dengan p=0,01 (P<0,05), yang menunjukkan adanya perbedaansensitivitas yang bermakna antara kloramfenikol, polimiksin-neomisin, ciprofloksasin dan ofloksasinterhadap Pseudomonas aeruginosa. Analisis post hoc menggunakan Mc Nemar menunjukkan bahwaterdapat perbedaan sensitivitas yang bermakna antara tetes telinga polimiksin-neomisin terhadapkloramfenikol, dan ciprofloksasin terhadap kloramfenikol p=0,000 (p<0,05), serta terdapat perbedaanyang bermakna antara tetes telinga ciprofloksasin terhadap ofloksasin, p=0,002, tetapi tidak terdapatperbedaan yang bermakna antara ciprofloksasin terhadap polimiksin-neomisin, p=0,687, polimiksinneomisinterhadap ofloksasin p=0,057, dan kloramfenikol terhadap ofloksasin p=0,109. Kesimpulan:Terdapat perbedaan sensitivitas yang bermakna tetes telinga antibiotik terhadap Pseudomonas aeruginosa pada pasien OMSK benigna aktif. Ciprofloksasin dan polimiksin-neomisin tetes telinga mempunyai sensitivitas yang lebih baik dibanding ofloksasin dan kloramfenikol. Kata kunci: Pseudomonas aeruginosa, otitis media supuratif kronik, tetes telinga antibiotik

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