scholarly journals Formulasi bioinsektisida Bacillus thuringiensis isolat indigenos untuk pengendalian Hyposidra talaca pada tanaman teh (Formulation of indigenous isolate of Bacillus thuringiensis bioinsecticide to control Hyposidra talaca on tea)

2019 ◽  
Vol 87 (1) ◽  
Author(s):  
Happy WIDIASTUTI ◽  
TRI - PANJI ◽  
Ciptadi Achmad YUSUP ◽  
Iman RUSMANA ◽  
Tri Eko WAHYONO
Keyword(s):  
Bacillus Thuringiensis ◽  
Integrated Pest Management ◽  
Pest Management ◽  
Phase Contrast ◽  
Phase Contrast Microscope ◽  
Contrast Microscope ◽  
Preliminary Study ◽  
Hyposidra Talaca ◽  
The One ◽  
White Clay

A study has been conducted to develop indigenousBacillusthuringiensisbioinsecticide. Preliminary study has been conducted to explore B. thuringiensisfrom sample of soil, leaf, and infected larvae from selected tea area as well as another area such as cocoa, and acasia. The result showed that based on the colony morphology, it was found 10 isolateswith the characteristics of B. thuringiensis colony.  Assessed of the ability to formed crystal using phase contrast microscope and staining revealed that 4 isolates as crystal protein forming isolates. The four isolates used as active coumpound of bioinsecticide. The best formula based on the viability of bacteria was the one withwhite clay as a carrier. While the best liquid formula based on the similar characteristic was using maltosa as osmoprotectant. Theassessment ofthetoxisity B. thuringiensistowards Hyposidra talacalarvae showed that B. thuringiensiscould control H. talacalarvaeup to 37.5%. However, the toxicity need longer periode compared to that commercial B. thuringiensisbioinsecticide. [Keywords:Bt insecticide, cypermethrine, integrated pest management, Tea looper].AbstrakPenelitian telah dilakukan untuk mengembangkan bioinsektisida berbahan aktif B. thuringiensisdari isolat asli Indonesia. Eksplorasi B. thuringiensisdari contoh berupa tanah, daun, dan ulat dari kebun teh telah dilakukan demikian pula dari habitat lain seperti kebun kakao dan akasia.  Berdasarkan morfologi koloni diperoleh 10 isolat yang menunjukkan ciri-ciri koloni B. thuringiensis. Selanjutnyaisolat yang diperoleh diuji kemampuan pembentukan kristal protein dengan pewarnaan dan pengamatan mikroskop phase kontras dan menghasilkan 4 isolat yang mampu membentuk kristal protein. Selanjutnya keempat isolat yang diperoleh digunakan sebagai bahan aktif dalam formulasi bioinsektisida. Formula terbaik berdasarkan kriteria viabilitas bakteri adalah formula yang menggunakan bahan pembawa berupa white clay. Formula terbaik untuk bioinsektida cair berdasarkan kriteria viabilitas B. thuringiensisdan kejernihan bioinsektisida adalah menggunakan maltosesebagai osmoprotektan.Pada pengujian toksisitas isolat B. thuringiensisterhadap larvaulat jengkal(Hyposidra talaca)menunjukkan bahwa B. thuringiensishasil percobaan dapat mengen-dalikan larva ulat jengkalhingga 37,5%. Namun demikian toksisitasnya memerlukan waktu yang lebih lama dibandingkan dengan bioinsektisida berbahan aktif B. thuringiensiskomersial. [Kata kuci: Bioinsektisida Bt, pengendalian hama terpadu, sipermetrin, ulat jengkal teh].

Histochemical and Morphological Studies of Lipids in the Oogenesis of Pheretima posthuma

1958 ◽  
Vol s3-99 (48) ◽  
pp. 475-484
Author(s):  
VISHWA NATH ◽  
BRIJ L. GUPTA ◽  
S. L. MANOCHA
Keyword(s):  
Phase Contrast ◽  
Chemical Change ◽  
Follicular Epithelium ◽  
Cholesteryl Esters ◽  
Lipid Bodies ◽  
Interference Microscope ◽  
Phase Contrast Microscope ◽  
Morphological Studies ◽  
Contrast Microscope ◽  
Second Category

A study of the oocytes of the earthworm, Pheretima posthuma, examined fresh under the phase-contrast and interference microscopes as well as by histochemical techniques, has revealed that there are two types of lipid bodies in the cytoplasm. The lipid bodies of the first type (L1) are smaller, appear as homogeneous, dark granules under the phase-contrast microscope, and have a protein-phospholipid core surrounded by a thick sheath of phospholipids only. The lipid bodies of the second category (L2), which arise as a result of growth and chemical change in L1 bodies, have a pure phospholipid core surrounded by a thick triglyceride sheath. They give a ringed appearance under the phase-contrast microscope. The study under the interference microscope shows that this ringed appearance is an optical artifact. The lipid spheres present in the follicular epithelium contain phospholipids only. The mitochondria are in the form of minute granules. They remain unchanged throughout oogenesis. Some vacuoles devoid of any lipids, proteins, or carbohydrates have been observed. They also remain unchanged. Pure triglyceride spheres, yolk globules, nucleolar extrusions, as well as cholesterols and cholesteryl esters are absent.

Redescription of Neelus fimbriatus Bretfeld & Trinklein, 2000 (Collembola: Neelidae) from Colombia

Zootaxa ◽  
2018 ◽  
Vol 4527 (3) ◽  
pp. 414
Author(s):  
ELIDA P. MARÍN ◽  
JOSÉ G. PALACIOS-VARGAS
Keyword(s):  
Phase Contrast ◽  
Phase Contrast Microscope ◽  
Contrast Microscope

Neelus fimbriatus is redescribed using specimens from Colombia. Drawings and phase contrast microscope photos of the species are used. New characters are used as tibiotarsal tuberculate setae and abdominal ventral acetabula. 

Methods of transplanting nuclei from single cultured cells to unfertilized frogs' eggs

Development ◽  
1970 ◽  
Vol 24 (2) ◽  
pp. 249-255
Author(s):  
J. B. Gurdon ◽  
R. A. Laskey
Keyword(s):  
Xenopus Laevis ◽  
Phase Contrast ◽  
Cultured Cells ◽  
The Other ◽  
Phase Contrast Microscope ◽  
Cell Method ◽  
Attached Cell ◽  
Contrast Microscope

Two methods of transplanting single nuclei from monolayers of cultured cells to unfertilized eggs of Xenopus laevis are described, illustrated, and tested. The detached-cell method is simpler and quicker to operate and is suitable for homogeneous populations of cells which are easily removed from the substrate on which they are growing. The other, attached-cell, method is technically more elaborate, but is applicable to cells whose properties can be individually determined under the phase-contrast microscope and to cells which are not readily dissociated from other cells or from their substrate.

scholarly journals Evaluation of Haematuria and Use of Phase Contrast Microscope: A Short Review

1970 ◽  
Vol 20 (1) ◽  
pp. 63-67 ◽  
Author(s):  
T Sultana ◽  
T Sultana ◽  
MQ Rahman ◽  
ANN Ahmed
Keyword(s):  
Phase Contrast ◽  
Diagnostic Marker ◽  
Short Review ◽  
Red Cells ◽  
Phase Contrast Microscopy ◽  
Red Cell ◽  
Phase Contrast Microscope ◽  
Non Invasive ◽  
Contrast Microscope ◽  
Contrast Microscopy

For centuries physicians have been using urine as one of the non-invasive means for assessing diseases. Haematuria is a frequently encountered abnormality in clinical practice. Haematuria may have either a glomerular or a non-glomerular origin. The morphological study of urinary red cells by Phase-Contrast Microscopy (PCM) is a useful diagnostic marker for glomerular bleeding, if correctly interpreted and used. Today, urinalysis and in particular identification of red cells morphology by Phase-Contrast Microscopy has been a widely accepted technique for determining the site of haematuria. A short review on haematuria and Phase-Contrast Microscopy are presented here for updating knowledge and academic interest. Key words: Phase-contrast microscope; Haematuria; Dysmorphic red cell. DOI: http://dx.doi.org/10.3329/jdmc.v20i1.8584 J Dhaka Med Coll. 2011; 20(1) :63-67

scholarly journals Qualities of goat semen in Tris-Citrate-Glucose extender containing glutathione

1970 ◽  
Vol 27 (2) ◽  
pp. 46-55 ◽  
Author(s):  
B Mishra ◽  
MGS Alam ◽  
MAMY Khandokar ◽  
S Mazumder ◽  
MN Munsi
Keyword(s):  
Sperm Motility ◽  
Artificial Insemination ◽  
Phase Contrast ◽  
Phase Contrast Microscope ◽  
Contrast Microscope ◽  
And Control ◽  
Control Samples

Glutathione (GSH) 0 (control), 2, 4 and 8 mM was used in the preservation of chilled goat semen. Treated and control samples were kept at 4 – 5°C up to seven days. Sperm motility and acrosome abnormality were assessed daily under phase contrast microscope. The sperm motility was significantly (P<0.01) higher in the semen treated with 8 mM GSH. Optimum sperm motility (≥50%) for artificial insemination was retained for three days with 2 and 4 mM GSH and up to four days with 8 mM GSH. Acrosomal damage was significantly (P<0.01) reduced to ≤ 1.0% after addition of 8 mM GSH. It is suggested that GSH may be used as an antioxidant for better preservation of goat semen for artificial insemination. DOI: 10.3329/bvet.v27i2.7554 Bangl. vet. 2010. Vol. 27, No. 2, 46 – 55

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