Changes in the Rapid Phase of Chlorophyll Fluorescence During Tomato Ripening

2021 ◽  
Vol 1 (42) ◽  
pp. 80-85
Author(s):  
Yuriy Sudnik ◽  
◽  
Mahmud Abdelhamid ◽  
Valeriya Strokina ◽  
◽  
...  

Sorting tomatoes by controlling their maturation is an important direction that characterizes the quality and marketability of tomatoes. Therefore, reliable control of the maturation of tomatoes is an actual and practically significant task. Currently, there are known methods of such control (RGB), based on determining the color of tomatoes. These methods have disadvantages, for example, insufficient reliability of monitoring the maturation of tomatoes of various (especially not contrasting in color) varieties, complexity and expensive equipment. (Research purpose) The research purpose is in proposing a new method of rapid sorting of tomatoes based on the control of the minimum (initial) level (Fo) of their rapid phase of chlorophyll fluorescence induction. The time of a single Fo measurement is from 0.01 to 0.07 seconds. (Materials and methods) The article presents mathematical models and conducted experimental studies for the fast phase of chlorophyll fluorescence induction, which confirmed the functional relationship between the maturation of tomatoes and their minimum (initial) level (Fo) of the fast phase of chlorophyll fluorescence induction. Authors used widely distributed botanical varieties of tomatoes "Alcazar", "Lezginka", "Rozanchik" with five stages of maturation (green, brown, pink, light red and red), for each of which we determined the key indicators of chlorophyll fluorescence induction: maximum (Fm), minimum (Fo), variable (Fv) values, the ratio of indicators (Fv/Fm). (Results and discussion) Chlorophyll fluorescence can be used to assess the maturation of tomatoes. As tomatoes ripen, the values of chlorophyll fluorescence parameters, such as Fo, Fm, Fv, and Fv/Fm, decrease. The article presents models that allowed us to estimate the stage of maturation of tomatoes for all three varieties. (Conclusions) The model that used both parameters of chlorophyll fluorescence was the best for three varieties of tomatoes.

2013 ◽  
Vol 52 (1-2) ◽  
pp. 149-152 ◽  
Author(s):  
Elżbieta Skórska

Naked oat variety of STH296 showed higher tolerance than traditional variety Bajka on short-term UV-B radiation (UV-B<sub>BE</sub>=1 1 kJ·m<sup>-2</sup>) on the stage of primary photosynthesis reaction recorded using chlorophyll fluorescence induction of the leaves.


Author(s):  
Yuriy A. Sudnik ◽  
Makhmud Abdelkhamid Abdelkhamid

The maturity of tomatoes serves as a basic parameter that determines their quality. Available maturity monitoring tools allow to measure the reflection coefficients of light from the surfaces of tomato fruits at certain wavelengths. These devices have the disadvantage of insufficient reliability in assessing the stages of maturity of tomatoes, they do not take into account their internal quality and structure. (Research purpose) The research purpose is in studying the dependence of the maturity of tomatoes on the level of slow induction of chlorophyll fluorescence. (Materials and methods) The article proposes a new method for separating tomato fruits according to their maturity, eliminating the inaccuracies of existing methods, based on the physical phenomenon of slow induction of chlorophyll fluorescence. During the experiment, three botanical varieties of tomatoes with different colors of mature fruits were used: “Alcazar” (red); “Wild rose” (pink); “Orange” (yellow). Five levels of maturity of single-sized tomato fruits were selected for each variety. Authors performed a variance analysis and comparison of the average values for the Duncan criterion with the significance level at α ≤ 0.05 using statistical analysis of the SPSS V. 20 software. (Results and discussion) The article presents general pattern for these tomato varieties: as the fruit matures, the value of their maximum fluorescence induction Fm and the coefficient of specific photosynthetic activity of tomatoes Kf decreases. The differences between the maturation stages with Fm and Kf are statistically significant at α ≤ 0.05. Regardless of the botanical variety, there are high negative correlations obtained for parameters of slow chlorophyll fluorescence induction, such as Fm and Kf with maturity. (Conclusions) The dependence of the decrease in the maximum fluorescence levels of chlorophyll with an increase in the maturity of tomato fruits was found. The proposed method allows to reliably separate tomato fruits with any color of mature fruits.


2014 ◽  
Vol 4 (1) ◽  
pp. 47-56 ◽  
Author(s):  
Luděk Sehnal ◽  
Peter Váczi ◽  
Miloš Barták

Trebouxia sp., a lichen symbiotic alga, was isolated from lichen Usnea antarctica collected at James Ross Island, Antarctica. After isolation, the alga was cultivated on Bold‘s Basal Medium (BBM-agar) with addition of nitrogen for 12 days. Growth of alga and its photosynthetic properties were studied in relation to increased concentration of CO2 (850±50 ppm) and two cultivation temperature (8 and 12°C). Physiological status of algae was evaluated by chlorophyll fluorescence parameters. Simultaneously, content of pigments and changes in biomass were evaluated during cultivation period. Evaluation of physiological state of Trebouxia sp. was carried out after the end of experiment. Results of chlorophyll fluorescence induction parameters and content of pigments showed that the highest efficiency of primary processes of photosynthesis was found at the treatment with elevated concentration of CO2 and temperature 12°C. In this treatment, potential quantum yield of photochemical processes in photosystem II (FV/FM) was 0.44 and content of Chl a was 5.14 µg ml-1. In contrast, the lowest efficiency of primary processes was found at the treatment with addition of CO2 and temperature 8°C, where value of FV/FM reached 0.37 and content of Chl a was 3.71 µg ml-1.


2010 ◽  
Vol 12 (4) ◽  
pp. 546-563 ◽  
Author(s):  
Esa Tyystjärvi ◽  
Michael Nørremark ◽  
Heta Mattila ◽  
Mika Keränen ◽  
Marja Hakala-Yatkin ◽  
...  

1985 ◽  
Vol 104 (3) ◽  
pp. 501-504 ◽  
Author(s):  
M. Havaux ◽  
R. Lannoye

SummaryDisks of hard wheat (Triticum durum Desf.) leaves subjected to rapid desiccation over 4 h showed noticeable changes in the shape of the in vivo chlorophyll fluorescence induction curves. In drought-sensitive varieties (such as Claridoc), water stress resulted in a strong inhibition of the slow fluorescence induction transients. In particular, the fluorescence quenching rate was markedly decreased in water-stressed leaf disks. In contrast, leaves of drought-resistant varieties (such as Aouedj) showed only minor changes in chlorophyll fluorescence. The results of this investigation suggest that the slow transient of the in vivo chlorophyll fluorescence induction phenomenon may provide a simple method for selecting drought-tolerant wheats.


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