scholarly journals Evaluation of T-Bet immunostaining in the counting of intraepithelial lymphocytes in celiac disease

2020 ◽  
Vol 6 (5) ◽  
Author(s):  
Amna E. Al-araji ◽  
Tuqa Z. Omran ◽  
Mohanad M. Ahmed ◽  
Nazar J. Metib
Keyword(s):  
Celiac Disease ◽  
Intraepithelial Lymphocytes ◽  
Clinicopathological Features ◽  
P Value ◽  
Formalin Fixed Paraffin ◽  
Histological Criteria ◽  
Formalin Fixed Paraffin Embedded ◽  
Potential Alternative ◽  
Almost All ◽  
Formalin Fixed

Objective: In this study, we aimed to evaluate CD3, T-bet and GATA3 staining of intraepithelial lymphocytes (IELs) in comparison to the routine H&E stains in celiac disease. Methods: a total of 50 patients, in whom celiac disease was diagnosed based on a combination of clinicopathological features, were enrolled in the study. Duodenal biopsied tissues were processed routinely into formalin fixed paraffin embedded (FFPE) blocks. Sections were prepared and stained with H&E and each of CD3, T-bet and GATA3. A number of histological criteria were measured to calculate the Marsh score. The results were analyzed using the IBM SPSS analytic software. Results: a positive correlation was found between the count of T-bet stained cells and the increase of intraepithelial lymphocytes (P-value = 0.001). In addition, low count of GATA3 stained cells was seen in almost all cases. The count of GATA3 stained cells was not affected by the increase in IELs count. Conclusions: the majority of increased IELs were stained with T-bet. Whereas in normal IELs count is less than half the IELs were stained with T-bet. This would indicate that T-bet immunostaining is a potential alternative to H&E and/or CD3 based counting of IELs.

scholarly journals Higher EU-TIRADS-Score Correlated with BRAF V600E Positivity in the Early Stage of Papillary Thyroid Carcinoma

2021 ◽  
Vol 10 (11) ◽  
pp. 2304
Author(s):  
Karolina Skubisz ◽  
Joanna Januszkiewicz-Caulier ◽  
Patrycja Cybula ◽  
Elwira Bakuła-Zalewska ◽  
Krzysztof Goryca ◽  
...  
Keyword(s):  
Early Stage ◽  
Braf V600e ◽  
P Value ◽  
Dna And Rna ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Ion Proton ◽  
Indolent Disease ◽  
Next Generation Sequencing Ngs ◽  
Formalin Fixed

The data demonstrating a correlation between sonographic markers of malignancy of thyroid cancer (TC) and its genetic status are scarce. This study aimed to assess whether the addition of genetic analysis at the preoperative step of TC patients’ stratification could aid their clinical management. The material consisted of formalin-fixed paraffin-embedded tumor fragments of 49 patients who underwent thyroidectomy during the early stages of papillary TC (PTC). Tumor DNA and RNA were subjected to next-generation sequencing (NGS) on Ion Proton using the Oncomine™ Comprehensive Assay panel. We observed a significant correlation between BRAF V600E and a higher EU-TIRADS score (p-value = 0.02) with a correlation between hypoechogenicity and taller-than-wide tumor shape in analysed patients. There were no other significant associations between the identified genetic variants and other clinicopathological features. For TC patient’s stratification, a strong suspicion of BRAF V600E negativity in preoperative management of TC patients could limit the over-treatment of asymptomatic, very low-risk, indolent disease and leave room for active surveillance.

scholarly journals Correction to: A New Intraepithelial γδ T-Lymphocyte Marker for Celiac Disease Classification in Formalin-Fixed Paraffin-Embedded (FFPE) Duodenal Biopsies

2020 ◽  
Author(s):  
Alina Popp ◽  
Juha Taavela ◽  
Paolo Graziano ◽  
Paola Parente ◽  
Claudia Covelli ◽  
...  
Keyword(s):  
Celiac Disease ◽  
T Lymphocyte ◽  
Disease Classification ◽  
Original Version ◽  
Formalin Fixed Paraffin ◽  
Author Group ◽  
Formalin Fixed Paraffin Embedded ◽  
Duodenal Biopsies ◽  
Formalin Fixed

The original version of the article unfortunately contained an error in the first name and the surname of the all authors in the author group. Below is the corrected first name and the surname of the authors.

scholarly journals Immunohistochemical expression of Cyclin D1 among Sudanese patients diagnosed with benign and malignant prostatic lesions

2019 ◽  
Author(s):  
Eiman Siddig Ahmed ◽  
Lubna S. Elnour ◽  
Rowa Hassan ◽  
Emmanuel Edwar Siddig ◽  
Mintu Elsa Chacko ◽  
...  
Keyword(s):  
Family History ◽  
Cyclin D1 ◽  
P Value ◽  
Low Grade ◽  
High Grade ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Embedded Blocks ◽  
History Of ◽  
Formalin Fixed

Abstract Objectives: Prostate cancer (PC) is common cancer worldwide. Several markers have been developed to differentiate between benign prostatic hyperplasia (BPH) from PC. A descriptive retrospective hospital-based study aimed at determining the expression of Cyclin D1 in BPH and PC. the study took place at different histopathology laboratories in Khartoum state, Sudan, from December 2016 to January 2019. Formalin-fixed paraffin-embedded blocks were sectioned and fixed in 3-aminopropyltriethoxysilane coated slides incubated into primary antibody for Cyclin D1. The assessment of immunoreactivity of Cyclin D1 of each section was done using the Gleason scoring system. Results: A total of 153 males’ prostate sections included in this study, of them, 120 (78.4%) were PC, and 33 (21.6%) were BPH. Their age ranged from 45 to 88 years, mean age was 66.19 ± 8.599. 142 (92.8%) did not have a family history of PC, while 11 (7.2%) patients reported having a family history. The Gleason scoring showed a total of 81 (52.9%) patients with high-grade and 39 (25.5%) with low-grade. 118 (97.5%) patients had PC showed positive results for Cyclin D1, while BPH was 3 (2.5%). P value < 0.001. Cyclin D1 staining was associated with high-grade Gleason score and perineural invasion, P value 0.001.

scholarly journals p16 Expression Correlates with Invasive Ocular Surface Squamous Neoplasms in HIV-Infected Mozambicans

2019 ◽  
Vol 6 (2) ◽  
pp. 123-128 ◽  
Author(s):  
Carla Carrilho ◽  
Chau Miu ◽  
Yeji Kim ◽  
Susan Karki ◽  
Alexandra Balmaceda ◽  
...  
Keyword(s):  
Ocular Surface ◽  
P Value ◽  
P16 Immunohistochemistry ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Intraepithelial Neoplasms ◽  
Hematoxylin And Eosin ◽  
Tissue Specimens ◽  
Formalin Fixed ◽  
P16 Expression

Background: p16 immunohistochemistry is widely used to diagnose human papillomavirus (HPV)-related squamous neoplasms of cervix, anogenital, head, and neck tissues. The incidence of these HPV-related squamous neoplasms is markedly increased in the HIV-infected population. Ocular surface squamous neoplasia (OSSN) is also more common in HIV-infected patients. However, the expression pattern of p16 in OSSN among HIV-infected patients is unclear. Here, we examined the expression of p16 in OSSN surgical excisions collected from a large HIV-infected cohort from ­Mozambique. Methods: OSSN surgical tissue specimens were collected from 75 Mozambican patients. Formalin-fixed, paraffin-embedded tissue blocks from these OSSNs were sectioned, stained with hematoxylin and eosin (H&amp;E), and p16 expression by immunohistochemistry. H&amp;E slides were reviewed to determine if OSSNs were noninvasive conjunctival intraepithelial neoplasms or invasive squamous cell carcinomas (SCC). Cases were classified as p16 positive or negative based on diffuse nuclear and cytoplasmic expression of p16 in neoplastic cells. Results: p16 positivity was found in a minority of OSSN cases (14/75). p16 positivity was significantly associated with the invasive SCC type of OSSN in HIV-infected patients (p value of 0.026). Conclusions: The majority of OSSNs in our HIV-infected cohort do not express p16. However, those cases that are p16-positive are significantly more likely to be the invasive SCC form of OSSN. We propose that p16 expression may identify more aggressive OSSNs in HIV-infected populations.

scholarly journals The Effect of PT Link and Waterbath Antigenic Retrieval Procedures On The Expression of Common Receptors In Breast Cancer Cases

2017 ◽  
Vol 12 (1) ◽  
pp. 1 ◽  
Author(s):  
Ahmed Abdelbadie ◽  
Elsadig Ahmed Adam ◽  
Manar Abdelrahman
Keyword(s):  
Breast Cancer ◽  
Water Bath ◽  
Antigen Retrieval ◽  
P Value ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Embedded Blocks ◽  
Weak Expression ◽  
Immunohistochemical Techniques ◽  
Formalin Fixed

<p><strong>Background:</strong> Antigen retrieval (AR) techniques play a major role in determining the quality and functional state of tissue proteins recovered from formalin fixed paraffin embedded blocks (FFPEB). Different techniques are used each with its merits and drawbacks.<br /><strong>Materials and Method:</strong> FFPEB from cases diagnosed as breast cancer were examined for the expression of estrogen- receptor (ER), progesterone receptor (PR) and HER2 by immunohistochemical techniques using two different AR methods.<br /><strong>Results:</strong> Fifty cases, 49 were from females, the age ranged from 28–85 years were examined. The expression of ER using water bath and PT link (pretreatment system) as antigen retrieval methods was equal, both methods showed 20(40%) positive cases and 30(60%) negative cases, whereas, the expression of PR was found positive in 18 cases (36%) and negative in 32 (64%) when water bath was used, in comparison to10 positive (20%) and 40 negative (80%) when PT link was used (P value 0.312).The expression of HER2 was as follows; water bath HER2 weak positive 20 cases (40%), moderate expression 10 cases (20%), overexpression 2 cases (4%). PT link weak expression 13 cases (26%), moderate expression 15 cases (30%), overexpression 4cases (8%); yet, the total number of negative cases and positive cases was equal for both methods (p-value 0.035).<br />Conclusion: Antigen retrieval methods were found to affect the expression of common receptors in breast cancer. The magnitude of this effect was found to be significant in PR and HER2 receptors, however, the different methods of antigen retrieval did not affect the expression of ER. The appropriate and suitable retrieval methods have to be chosen individually for each receptor.</p><p><br /><strong>Keywords:</strong> Antigen retrieval, ER, PR, HER2 expression, PT link, waterbath.</p>

scholarly journals Lack of KBTBD4 Mutations in Molecularly Classified Brazilian Medulloblastomas

2019 ◽  
Vol 78 (9) ◽  
pp. 788-790 ◽  
Author(s):  
Letícia Ferro Leal ◽  
Rodrigo de Oliveira Cavagna ◽  
Nathalia Cristina Campanella ◽  
Bruna Mançano ◽  
Gisele Caravina Almeida ◽  
...  
Keyword(s):  
Sanger Sequencing ◽  
Molecular Subtype ◽  
Clinicopathological Features ◽  
Histological Subtype ◽  
Childhood Brain Tumors ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Hotspot Mutations ◽  
Formalin Fixed

Abstract Medulloblastoma is the most frequent malignant brain tumor in children, representing 20% of all childhood brain tumors. Currently, medulloblastomas are molecularly classified in 4 subgroups that are associated with distinctive clinicopathological features. KBTBD4 mutations were recently described in a subset of MBGRP3 and MBGRP4 medulloblastomas subgroups. However, no other studies reported KBTBD4 mutations in medulloblastomas. Thus, our aim was to investigate KBTBD4 mutations in a Brazilian series of medulloblastoma. We evaluated 128 medulloblastoma patients molecularly classified from 4 Brazilian reference centers. DNA from formalin-fixed, paraffin-embedded samples was screened for KBTBD4 hotspot mutations by Sanger sequencing. Most of the patients were male, average age was 16.5 years old and average overall survival was 55.9 months. The predominant histological subtype was the classic subtype, followed by nodular/desmoplastic, and the predominant medulloblastoma molecular subtype was the MBSHH subgroup (46%), followed by MBGRP3 and MBGRP4 (19%/each), and MBWNT (16%). Among the 128 samples, 111 were successfully sequenced. No KBTBD4 mutations were identified in 111 samples. Our findings suggest that KBTBD4 mutations are uncommon in Brazilian MBGRP3 and MBGRP4 medulloblastomas subgroups. Further studies in a larger series of MBGRP3 and MBGRP4 medulloblastomas are warranted to better assess role of KBTBD4 mutations.

Anti-TCR gamma antibody in celiac disease: the value of count on formalin-fixed paraffin-embedded biopsies.

2013 ◽  
Vol 463 (3) ◽  
pp. 409-413 ◽  
Author(s):  
Silvia Lonardi ◽  
Vincenzo Villanacci ◽  
Luisa Lorenzi ◽  
Alberto Lanzini ◽  
Francesco Lanzarotto ◽  
...  
Keyword(s):  
Celiac Disease ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Formalin Fixed

scholarly journals Immunohistochemical expression of Cyclin D1 among Sudanese patients diagnosed with benign and malignant prostatic lesions

2020 ◽  
Author(s):  
Eiman Siddig Ahmed ◽  
Lubna S. Elnour ◽  
Rowa Hassan ◽  
Emmanuel Edwar Siddig ◽  
Mintu Elsa Chacko ◽  
...  
Keyword(s):  
Family History ◽  
Cyclin D1 ◽  
P Value ◽  
Low Grade ◽  
High Grade ◽  
Formalin Fixed Paraffin ◽  
Formalin Fixed Paraffin Embedded ◽  
Embedded Blocks ◽  
History Of ◽  
Formalin Fixed

Abstract Objectives: Prostate cancer (PC) is common cancer worldwide. Several markers have been developed to differentiate between benign prostatic hyperplasia (BPH) from PC. A descriptive retrospective hospital-based study aimed at determining the expression of Cyclin D1 in BPH and PC. the study took place at different histopathology laboratories in Khartoum state, Sudan, from December 2016 to January 2019. Formalin-fixed paraffin-embedded blocks were sectioned and fixed in 3-aminopropyltriethoxysilane coated slides incubated into primary antibody for Cyclin D1. The assessment of immunoreactivity of Cyclin D1 of each section was done using the Gleason scoring system. Results: A total of 153 males’ prostate sections included in this study, of them, 120 (78.4%) were PC, and 33 (21.6%) were BPH. Their age ranged from 45 to 88 years, mean age was 66.19 ± 8.599. 142 (92.8%) did not have a family history of PC, while 11 (7.2%) patients reported having a family history. The Gleason scoring showed a total of 81 (52.9%) patients with high-grade and 39 (25.5%) with low-grade. 118 (97.5%) patients had PC showed positive results for Cyclin D1, while BPH was 3 (2.5%). P value < 0.001. Cyclin D1 staining was associated with high-grade Gleason score and perineural invasion, P value 0.001.

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