scholarly journals Accurate and Rapid Methods for Detecting Salmonella spp. Using Polymerase Chain Reaction and Aptamer Assay from Dairy Products: A Review

2020 ◽  
Vol 38 (4) ◽  
pp. 169-188
Author(s):  
Ji-Yeon Hyeon ◽  
Kun-Ho Seo ◽  
Jung-Whan Chon ◽  
Dongryeoul Bae ◽  
Dongkwan Jeong ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Dairy Products ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Rapid Methods ◽  
Aptamer Assay ◽  
Polymerase Chain

Polymerase Chain Reaction Detection of Salmonella spp. in Fecal Samples of Pet Birds

2008 ◽  
Vol 3 (1) ◽  
pp. e29-e29
Author(s):  
B. Sareyyüpoğlu ◽  
A Çelik Ok ◽  
Z. Cantekin ◽  
H. Yardimci ◽  
M. Akan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Fecal Samples ◽  
Pet Birds ◽  
Polymerase Chain

Development of a Multiplex Polymerase Chain Reaction Assay for Detection and Differentiation of Staphylococcus aureus in Dairy Products†

2001 ◽  
Vol 64 (5) ◽  
pp. 664-668 ◽  
Author(s):  
SUDHIR TAMARAPU ◽  
JOHN L. McKILLIP ◽  
MARYANNE DRAKE
Keyword(s):  
Staphylococcus Aureus ◽  
Polymerase Chain Reaction ◽  
Multiplex Pcr ◽  
Dairy Products ◽  
Multiplex Polymerase Chain Reaction ◽  
Skim Milk ◽  
Cheddar Cheese ◽  
Polymorphism Analysis ◽  
Chain Reaction ◽  
Polymerase Chain

A multiplex polymerase chain reaction (PCR) assay was developed for the detection and differentiation of enterotoxigenic Staphylococcus aureus in dairy products. A solvent extraction procedure was successfully modified for extraction of S. aureus DNA from 10 ml of artificially contaminated skim milk or 20 g cheddar cheese. Primers targeting the enterotoxin C gene (entC) and thermostable nuclease gene (nuc) were used in the multiplex PCR. PCR products were confirmed using restriction fragment length polymorphism analysis. DNA was consistently quantified and amplified by uniplex PCR from 10 CFU/ml of S. aureus in skim milk or 10 CFU/20 g cheddar cheese. The sensitivity of the multiplex PCR was 100 CFU/ml of skim milk or 100 CFU/20 g cheddar cheese. The developed methodology allows presumptive identification and differentiation of enterotoxigenic S. aureus in less than 6 h.

scholarly journals Validation of Droplet Digital Polymerase Chain Reaction for Salmonella spp. Quantification

2020 ◽  
Vol 11 ◽  
Author(s):  
Carolina Villamil ◽  
Martha Nancy Calderon ◽  
Maria Mercedes Arias ◽  
John Emerson Leguizamon
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Polymerase Chain ◽  
Digital Polymerase Chain Reaction

Rapid Methods for Quality Assurance of Foods: the Next Decade with Polymerase Chain Reaction (PCR)-Based Food Monitoring

2014 ◽  
Vol 8 (2) ◽  
pp. 255-271 ◽  
Author(s):  
D. De Medici ◽  
T. Kuchta ◽  
R. Knutsson ◽  
A. Angelov ◽  
B. Auricchio ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Quality Assurance ◽  
Chain Reaction ◽  
Rapid Methods ◽  
Food Monitoring ◽  
Polymerase Chain

Combination of Immunomagnetic Separation and Polymerase Chain Reaction for the Simultaneous Detection of Listeria monocytogenes and Salmonella spp. in Food Samples

2001 ◽  
Vol 64 (11) ◽  
pp. 1744-1750 ◽  
Author(s):  
HSIEN-YEE HSIH ◽  
HAU-YANG TSEN
Keyword(s):  
Polymerase Chain Reaction ◽  
Listeria Monocytogenes ◽  
Simultaneous Detection ◽  
Meat Products ◽  
Immunomagnetic Separation ◽  
Food Samples ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Pcr Method ◽  
Polymerase Chain

A method that combined the immunomagnetic separation (IMS) technique and the multiplex polymerase chain reaction (PCR) method (i.e., the IMS-mPCR method) was developed for simultaneous detection of Listeria monocytogenes and Salmonella spp. in food samples. When only the multiplex PCR method was used, it was found that if cell numbers of each of the two target organisms (L. monocytogenes and Salmonella spp.) were above the detection limit, but differed by more than 2 logs—e.g., n × 107 to n × 104 or n × 106 to n × 103—the organism presenting the lower numbers might go undetected. Following the enrichment step with universal preenrichment (UP) broth, if an IMS method using equal quantities of anti-Listeria and anti-Salmonella immunomagnetic beads was performed prior to PCR, both pathogens could be detected unambiguously. Such results could be obtained for target organisms in food samples, such as milk, dairy, and meat products, if similar enrichment and IMS steps were performed prior to PCR.

Detection of Salmonella spp. in tropical seafood by polymerase chain reaction

2003 ◽  
Vol 88 (1) ◽  
pp. 91-95 ◽  
Author(s):  
H. Sanath Kumar ◽  
R. Sunil ◽  
M.N. Venugopal ◽  
Indrani Karunasagar ◽  
Iddya Karunasagar
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Polymerase Chain
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