Efficacy of a commercial polymerase chain reaction-based assay for detection of Salmonella spp. in animal feeds

2000 ◽  
Vol 89 (4) ◽  
pp. 710-718 ◽  
Author(s):  
K.G. Maciorowski ◽  
S.D. Pillai ◽  
S.C. Ricke
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Animal Feeds ◽  
Polymerase Chain

Polymerase Chain Reaction Detection of Salmonella spp. in Fecal Samples of Pet Birds

2008 ◽  
Vol 3 (1) ◽  
pp. e29-e29
Author(s):  
B. Sareyyüpoğlu ◽  
A Çelik Ok ◽  
Z. Cantekin ◽  
H. Yardimci ◽  
M. Akan ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Polymerase Chain Reaction Detection ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Fecal Samples ◽  
Pet Birds ◽  
Polymerase Chain

scholarly journals Validation of Droplet Digital Polymerase Chain Reaction for Salmonella spp. Quantification

2020 ◽  
Vol 11 ◽  
Author(s):  
Carolina Villamil ◽  
Martha Nancy Calderon ◽  
Maria Mercedes Arias ◽  
John Emerson Leguizamon
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Polymerase Chain ◽  
Digital Polymerase Chain Reaction

Combination of Immunomagnetic Separation and Polymerase Chain Reaction for the Simultaneous Detection of Listeria monocytogenes and Salmonella spp. in Food Samples

2001 ◽  
Vol 64 (11) ◽  
pp. 1744-1750 ◽  
Author(s):  
HSIEN-YEE HSIH ◽  
HAU-YANG TSEN
Keyword(s):  
Polymerase Chain Reaction ◽  
Listeria Monocytogenes ◽  
Simultaneous Detection ◽  
Meat Products ◽  
Immunomagnetic Separation ◽  
Food Samples ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Pcr Method ◽  
Polymerase Chain

A method that combined the immunomagnetic separation (IMS) technique and the multiplex polymerase chain reaction (PCR) method (i.e., the IMS-mPCR method) was developed for simultaneous detection of Listeria monocytogenes and Salmonella spp. in food samples. When only the multiplex PCR method was used, it was found that if cell numbers of each of the two target organisms (L. monocytogenes and Salmonella spp.) were above the detection limit, but differed by more than 2 logs—e.g., n × 107 to n × 104 or n × 106 to n × 103—the organism presenting the lower numbers might go undetected. Following the enrichment step with universal preenrichment (UP) broth, if an IMS method using equal quantities of anti-Listeria and anti-Salmonella immunomagnetic beads was performed prior to PCR, both pathogens could be detected unambiguously. Such results could be obtained for target organisms in food samples, such as milk, dairy, and meat products, if similar enrichment and IMS steps were performed prior to PCR.

Detection of Salmonella spp. in tropical seafood by polymerase chain reaction

2003 ◽  
Vol 88 (1) ◽  
pp. 91-95 ◽  
Author(s):  
H. Sanath Kumar ◽  
R. Sunil ◽  
M.N. Venugopal ◽  
Indrani Karunasagar ◽  
Iddya Karunasagar
Keyword(s):  
Polymerase Chain Reaction ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Polymerase Chain

Evaluation of a Polymerase Chain Reaction–Based Test for Detecting Salmonella spp. in Food Samples: Probelia Salmonella spp.

1999 ◽  
Vol 62 (12) ◽  
pp. 1387-1393 ◽  
Author(s):  
PATRICK FACH ◽  
FRANÇOISE DILASSER ◽  
JOËL GROUT ◽  
JOCELYNE TACHE
Keyword(s):  
Polymerase Chain Reaction ◽  
Limit Of Detection ◽  
Collaborative Study ◽  
Reference Method ◽  
Food Samples ◽  
Complete Agreement ◽  
Chain Reaction ◽  
Salmonella Spp ◽  
Sandwich Hybridization ◽  
Polymerase Chain

A commercially available polymerase chain reaction (PCR) kit was evaluated for the detection of Salmonella spp. in food samples. The test combines PCR amplification and sandwich hybridization of the amplified DNA in microtiter plates. The sensitivity and specificity was evaluated with 52 Salmonella strains and 51 non-Salmonella strains and showed that the test was entirely reliable. The threshold sensitivity was 102 CFU/ml. The limit of detection of dead cells that determines the minimum detection level of dead cells in food samples was superior to 106 CFU/25 g, a level rarely achieved in naturally contaminated samples. After an 18-h pre-enrichment step, the test could detect viable Salmonella in artificially contaminated food samples, even for the lower contamination level (3 CFU/25 g). There was complete agreement between the PCR test and the ISO 6579 bacteriological reference method with artificially contaminated samples. Regarding the accuracy of the results obtained from 253 naturally or noncontaminated foods and from 32 artificially contaminated foods, the agreement percentage was 99.6%. The fidelity of the technique was evaluated in a collaborative study with eight European laboratories and showed a correlation of 98.4%.

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