scholarly journals Spoligotyping, Resistensi Mycobacterium tuberculosis terhadap obat TB dan Faktor Risiko Penularan Tuberkulosis Paru di Kota Jayapura

2019 ◽  
Vol 47 (1) ◽  
pp. 1-12
Author(s):  
Hana Krismawati ◽  
Antonius Oktavian ◽  
Lydia Chaidir ◽  
Evy Iriani Natalia ◽  
Melda Suebu ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Tuberculosis Patients ◽  
Tuberculosis Transmission ◽  
Mycobacterium Growth Indicator Tube ◽  
Indicator Tube ◽  
Solid Media ◽  
Mdr Tb ◽  
Close Contacts ◽  
Growth Indicator

Identification of the Mycobacterium tuberculosis strain found in pulmonary tuberculosis patients in Jayapura using Spolygotyping was carried out Patients with pulmonary tuberculosis are taken as an index case. Sputum samples were taken to be diagnosed with Acid Fast Staining and to culture M. tuberculosis bacteria using Lowensten Jansen solid media, Ogawa solid media and Mycobacterium Growth Indicator Tube liquid media. The patient's home visit was conducted to determine the environmental conditions of the patient's residence, interview the close contacts and test the bacterial infection M. tuberculosis in close contact cases using the Mantoux test. A thorax radiology of close contacts was carried out in the hospital's radiology unit. The results of spolygotyping showed 8%sputum samples M. tuberculosis of Beijing strain, while the rest are M. tuberculosis non Beijing strains. Among the 46 of Non-Beijing strains, the type U (likely S) is most transmitted by tuberculosis patients to their close contacts, which is as much as 6%. This study confirms the factors that influence tuberculosis transmission are the sleeping location of close contacts with the patients, the frequency of meetings that occur at least once or more in a day, density of house occupants and the presence of clinical symptoms of TB. Drugs Resistance Susceptibility test results showed that 12 patients were resistance to TB drugs and one of them was MDR TB from strain LAMP. Key words : tuberculosis transmission, Spolygotyping, tuberculosis, Jayapura, drugs resistance   Abstrak Identifikasi galur Mycobacterium tuberculosis yang ditemukan pada penderita tuberculosis paru di Jayapura telah dilakukan dengan menggunakan Spoligotyping Penderita tuberkulosis paru diambil sebagai kasus indeks. Sampel sputum juga diambil untuk didiagnosis dengan pengecatan Basil Tahan Asam dan kultur bakteri M.tuberculosis yang dilakukan pada media padat Lowensten Jansen, media padat Ogawa serta media cair Mycobacterium Growth Indicator Tube. Ekstraksi DNA dilakukan pada koloni Mycobacterium tuberculosis untuk dilakukan analisis spoligotyping. Kunjungan rumah pasien dilakukan untuk mengetahui kondisi lingkungan tempat tinggal pasien, mewawancara kontak kasus dan melakukan skrining infeksi bakteri M.tuberkulosis pada kasus kontak menggunakan tes Mantoux. Foto dada pada kontak kasus selanjutnya dilakukan di unit radiologi rumah sakit. Hasil spoligotyping menunjukkan 8% (4/50) sampel sputum penderita tuberkulosis adalah M.tuberculosis galur Beijing, sedangkan sisanya adalah M.tuberculosis galur Non Beijing. Dari 46 galur Non-Beijing, tipe U (likely S) paling banyak ditransmisikan oleh penderita tuberculosis pada kontaknya yaitu sebanyak 6% (3/50). Penelitian ini mengkonfirmasi faktor-faktor yang mempengaruhi transmisi tuberkulosis meliputi lokasi tidur kontak kasus yang serumah dengan penderita, frekuensi pertemuan yang terjadi sedikitnya sekali atau lebih dalam sehari, kondisi rumah padat penghuni dan adanya gejala klinis TB. Hasil uji resistensi menunjukkan 12 pasien mengalami resistensi terhadap OAT dan 1 diantaranya adalah MDR TB dari strain LAMP. Kata kunci : Transmisi, Splygotyping, Mycobacterium tuberculosis, tuberkulosis, Jayapura, resistensi

scholarly journals Comparison of Line Probe Assay (LPA) and Mycobacterium Growth Indicator Tubes (MGIT) Assay for Second-line TB Drug Susceptibility Testing

2021 ◽  
Vol 13 (3) ◽  
pp. 256-60
Author(s):  
Towifah Fauziah Choerunisa ◽  
Leni Lismayanti ◽  
Tiene Rostini ◽  
Ryan Bayusantika ◽  
Ida Parwati
Keyword(s):  
Susceptibility Testing ◽  
Multidrug Resistant ◽  
Second Line ◽  
Line Probe Assay ◽  
Mycobacterium Growth Indicator Tube ◽  
Indicator Tube ◽  
Significant Difference ◽  
Mdr Tb ◽  
Probe Assay ◽  
Growth Indicator

BACKGROUND: Tuberculosis (TB) infection is one of the most prominent health issues in the world, including in Indonesia. TB is evolving into multidrug-resistant tuberculosis (MDR-TB) and requiring second-line TB drugs. Mycobacterium growth indicator tube (MGIT) is the gold standard for susceptibility testing of second-line TB drugs. Alternatively, line probe assay (LPA), which detects genes resistant to second-line TB drugs, takes a shorter time to run. This study aims to compare MGIT and LPA's ability to detect TB resistance to second-line TB drugs and observe mutation patterns of genes encoding second-line TB drugs.METHODS: This was an observational analytic study, using cross-sectional method. The data were acquired from the MDR-TB clinic’s medical records at the Dr. Hasan Sadikin Hospital from September to December 2019. LPA and MGIT test were conducted at the Health Laboratory Hall of West Java Province, then tested using Kolmogorov-Smirnov and chi-square statistic.RESULTS: From 121 subjects, 113 people were not resistant to the second-line TB drugs, which was examined using both LPA and MGIT (93.4%), p=0.991. Mutations were found in gyrA and rrs gene. There was no significant difference between the proportion of subjects resistant to the second-line of TB drugs tested using LPA and MGIT.CONCLUSION: LPA is an alternative method to MGIT because it requires a shorter time and reduces the risk of exposure that will improve MDR-TB patients management.KEYWORDS: line probe assay (LPA), multidrug-resistant TB, mycobacterium growth indicator tube (MGIT), second-line TB drugs 

scholarly journals Resistance patterns of Mycobacterium tuberculosis isolates from pulmonary tuberculosis patients in Nairobi

2011 ◽  
Vol 6 (01) ◽  
pp. 33-39 ◽  
Author(s):  
Perpetual Wangui Ndung'u ◽  
Samuel Kariuki ◽  
Zipporah Ng'ang'a ◽  
Gunturu Revathi
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Small Businesses ◽  
Drug Susceptibility ◽  
Multidrug Resistant ◽  
First Line ◽  
Tuberculosis Patients ◽  
Control Programs ◽  
Solid Media ◽  
Resistance Patterns

Introduction: In Kenya, which ranks thirteenth of 27 high tuberculosis burden countries, diagnosis is based on Ziehl-Neelsen staining alone and patients are treated without information on sensitivity patterns. This study aimed to determine resistance patterns of Mycobacterium tuberculosis isolated from pulmonary samples.Methodology: Pulmonary tuberculosis patients in Nairobi were randomly sampled after informed consent and recruited into the study using a structured questionnaire. Specimens were cultured in liquid and solid media, and drug susceptibility tests were performed for first-line drugs including (isoniazid, rifampin, streptomycin, ethambutol and pyrazinamide). Results: Eighty-six (30%) of 286 isolates were resistant to at least one of five antibiotics tested. Thirty-seven (30.2%) isolates were resistant to isoniazid; 15 (11.6%) to streptomycin; 13 (4.5%) to ethambutol; four (1.4%) to rifampin ; and 30 (10.4%) to pyrazinamide. Double resistance was seen as follows: four (1.4%) isolates were resistant to both isoniazid and pyrazinamide; four (1.4%) to streptomycin and isoniazid; and one (0.3%) to rifampin and streptomycin. Two isolates (0.7%) were multidrug resistant, and one was triple resistant with an additional resistance to ethambutol. Results also showed 88.7% of patients were below the age of 40 years, while 26.3% were HIV positive. The majority of the patients (66.5%) were unemployed or self-employed in small businesses, with 79.4% earning less than 100 USD per month.Conclusion: The high resistance observed in isoniazid, which is a first-line drug, could result in an increase in multidrug resistance unless control programs are strengthened. Poverty should be addressed to reduce infection rates.

scholarly journals Failure of BACTEC™ MGIT 960™ to detect Mycobacterium tuberculosis complex within a 42-day incubation period

2017 ◽  
Vol 6 (1) ◽  
Author(s):  
Sharana Mahomed ◽  
Nomonde R. Dlamini-Mvelase ◽  
Moses Dlamini ◽  
Koleka Mlisana
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Retrospective Study ◽  
Incubation Period ◽  
Optimal Recovery ◽  
Tuberculosis Complex ◽  
Mycobacterium Growth Indicator Tube ◽  
Indicator Tube ◽  
Detection Failure ◽  
Growth Detection ◽  
Growth Indicator

For the optimal recovery of Mycobacterium tuberculosis from the BACTEC™ Mycobacterium Growth Indicator Tube 960™ system, an incubation period of 42–56 days is recommended by the manufacturer. Due to logistical reasons, it is common practice to follow an incubation period of 42 days. We undertook a retrospective study to document positive Mycobacterium Growth Indicator Tube cultures beyond the 42-day incubation period. In total, 98/110 (89%) were positive for M. tuberculosis complex. This alerted us to M. tuberculosis growth detection failure at 42 days.

scholarly journals Comparative study of different methods of microscopy followed by a culture method in the detection of mycobacterium tuberculosis in clinically and radiologically suspected cases of pulmonary tuberculosis

2021 ◽  
Vol 7 (2) ◽  
pp. 108-115
Author(s):  
Aarthi Sridhar ◽  
Anjana Gopi ◽  
Abhilasha Dalal ◽  
Divya Ravi
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Culture Method ◽  
Drug Susceptibility ◽  
Cross Sectional Study ◽  
Drug Susceptibility Testing ◽  
Cross Sectional ◽  
Indicator Tube ◽  
Resistant Tuberculosis ◽  
Mdr Tb

To compare the sensitivity of 2 microscopic methods for the diagnosis of Mycobacterium tuberculosis (M.tb) along with culture and drug susceptibility testing to first line drugs.: The cross-sectional study comprises 200 suspected cases of pulmonary tuberculosis both clinically and radiologically in KIMS, Bangalore over a period of 2 years. Samples (sputum/BAL fluid) were collected, processed and stained by Ziehl Neelson (ZN) and Fluorescent methods. Culture and drug susceptibilty testing was done for Streptomycin, Isoniazid, Rifampicin and Ethambutol by Mycobacterium growth indicator tube (MGIT) method after decontamination.Fischer’s test : 1. Out of 200 samples: 1.120 were male and 80 were female; 2. 18 were positive by Ziehl Neelson, 21 by Fluorescent and 28 by culture; 3. Majority of the patients belonged to age group 41-50 years (23%); 4. InMGIT, 26 were M.tb and 2 were Non-tubercular mycobacteria; 5. Out of 26 M.tb isolates, 4 were resistant to streptomycin, 6 to isoniazid, 2 to rifampicin and 9 to ethambutol.1. The sensitivity of Fluorescent staining (64.28%) is higher than that of Ziehl-Neelson (51.7%); 2. In MGIT, 26 were M.tb and 2 were Non tubercular mycobacteria; 3. 2 were Multi-drug resistant- tuberculosis (MDR-TB) This study made us aware of the need for prompt detection, identification and appropriate treatment of Tuberculosis due to the rising incidence of MDR-TB.

Two Liquid Medium Systems, Mycobacteria Growth Indicator Tube and MB Redox Tube, for Mycobacterium tuberculosis Isolation from Sputum Specimens

2000 ◽  
Vol 38 (3) ◽  
pp. 1227-1230 ◽  
Author(s):  
L. Heifets ◽  
T. Linder ◽  
T. Sanchez ◽  
D. Spencer ◽  
J. Brennan
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Liquid Medium ◽  
Growth Index ◽  
Indicator Tube ◽  
Solid Media ◽  
Valuable Element ◽  
Time To Detection ◽  
Culture Positive ◽  
Laboratory Protocol ◽  
Growth Indicator

Two manual liquid medium systems, the Mycobacteria Growth Indicator Tube (MGIT) and MB Redox tube systems, were evaluated in comparison to the radiometric BACTEC-460 semiautomated system for recovery ofMycobacterium tuberculosis from sputum specimens. The highest level of recovery, from a total of 77 culture-positive specimens, occurred with the BACTEC-460 system (92.2%), followed by the MB Redox tube (80.5%) and the MGIT (63.6%) systems. The shortest time to detection was observed also among the cultures in BACTEC-460: a mean of 12 days to a growth index (GI) of 10 and 15 days to a GI of 500. The mean times for the other systems were 16 days for the MB Redox tube system and 17.4 days for the MGIT system. The proportion of cultures grown after more than 3 weeks of incubation was only 2.8 or 8.4% in BACTEC-460 (for a GI of 10 or 500) but 17.7% in MB Redox and 22.5% in MGIT. Despite these differences in comparison to the BACTEC-460 system and some differences between the MGIT and MB Redox tube systems, either of the two manual liquid medium systems presents a reasonable alternative to the BACTEC-460 system, especially for laboratories with a limited workload, and a valuable element in the laboratory protocol, in conjunction with solid media, for obtaining rapid detection of growth from about 80% of culture-positive specimens and for better overall recovery of M. tuberculosis.

scholarly journals Kanamycin Susceptibility Testing of Mycobacterium tuberculosis Using Mycobacterium Growth Indicator Tube and a Colorimetric Method

2001 ◽  
Vol 45 (6) ◽  
pp. 1934-1936 ◽  
Author(s):  
Ivan Bastian ◽  
Leen Rigouts ◽  
Juan Carlos Palomino ◽  
Françoise Portaels
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Susceptibility Testing ◽  
Colorimetric Method ◽  
Alamar Blue ◽  
Mycobacterium Growth Indicator Tube ◽  
Indicator Tube ◽  
Proportion Method ◽  
Susceptibility Tests ◽  
Growth Indicator

ABSTRACT Two novel systems were evaluated for performing indirect kanamycin susceptibility tests on 72 strains of Mycobacterium tuberculosis. The microplate Alamar blue colorimetric method (breakpoint, 2.5 μg/ml) and the Mycobacterium Growth Indicator Tube (MGIT) system (breakpoint, 5.0 μg/ml) both produced 98.6% agreement when compared with the conventional proportion method performed on 7H10 agar using 5.0 μg of kanamycin/ml. Both systems provided results within an average of 1 week.

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