Two Liquid Medium Systems, Mycobacteria Growth Indicator Tube and MB Redox Tube, for Mycobacterium tuberculosis Isolation from Sputum Specimens

2000 ◽  
Vol 38 (3) ◽  
pp. 1227-1230 ◽  
Author(s):  
L. Heifets ◽  
T. Linder ◽  
T. Sanchez ◽  
D. Spencer ◽  
J. Brennan
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Liquid Medium ◽  
Growth Index ◽  
Indicator Tube ◽  
Solid Media ◽  
Valuable Element ◽  
Time To Detection ◽  
Culture Positive ◽  
Laboratory Protocol ◽  
Growth Indicator

Two manual liquid medium systems, the Mycobacteria Growth Indicator Tube (MGIT) and MB Redox tube systems, were evaluated in comparison to the radiometric BACTEC-460 semiautomated system for recovery ofMycobacterium tuberculosis from sputum specimens. The highest level of recovery, from a total of 77 culture-positive specimens, occurred with the BACTEC-460 system (92.2%), followed by the MB Redox tube (80.5%) and the MGIT (63.6%) systems. The shortest time to detection was observed also among the cultures in BACTEC-460: a mean of 12 days to a growth index (GI) of 10 and 15 days to a GI of 500. The mean times for the other systems were 16 days for the MB Redox tube system and 17.4 days for the MGIT system. The proportion of cultures grown after more than 3 weeks of incubation was only 2.8 or 8.4% in BACTEC-460 (for a GI of 10 or 500) but 17.7% in MB Redox and 22.5% in MGIT. Despite these differences in comparison to the BACTEC-460 system and some differences between the MGIT and MB Redox tube systems, either of the two manual liquid medium systems presents a reasonable alternative to the BACTEC-460 system, especially for laboratories with a limited workload, and a valuable element in the laboratory protocol, in conjunction with solid media, for obtaining rapid detection of growth from about 80% of culture-positive specimens and for better overall recovery of M. tuberculosis.

scholarly journals Comparison of MGIT 960 with Lowenstein Jensen Media for Recovery of Mycobacteria from Extrapulmonary Specimens in Southern India

2021 ◽  
Author(s):  
Kalpana Thangavelu ◽  
Imola Jamir ◽  
Kalaiarasan Ellappan ◽  
Krishnapriya Krishnakumariamma ◽  
Pallam Gopichand ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Extrapulmonary Tuberculosis ◽  
Tertiary Care ◽  
Smear Microscopy ◽  
Care Hospital ◽  
Lowenstein Jensen ◽  
Time To Detection ◽  
Culture Positive ◽  
Indicator Tubes ◽  
Growth Indicator

Introduction: Prevalence of Extrapulmonary Tuberculosis (EPTB) due to Mycobacteriumtuberculosis and Non-Tuberculous Mycobacteria (NTM) are on the rise especially in a developing country like India. Smear Microscopy (SM) is commonly used for detection of mycobacteria. Due to the paucibacillary nature in the extrapulmonary specimens SM pose a problem in detection. Though molecular methods are increasingly used now-a-days but there are possibilities that these reactions may get inhibited due to the presence of inhibitors in the extrapulmonary specimens. Aim: To compare Mycobacterium Growth Indicator Tubes (MGIT 960) with Lowenstein Jensen (LJ) medium for the detection of mycobacteria. Materials and Methods: The current prospective study was conducted on 1879 extrapulmonary specimens collected from a tertiary care hospital during the study period from July 2018 to March 2020. Specimens were subjected to Ziehl Neelsen (ZN) staining and Auramine Phenol (AP) staining. Culture was done in both LJ media and MGIT 960 culture. Positive mycobacterial cultures were subjected to MPT64 Immunochromatographic Test (ICT). Data were analysed using the Statistical Package for Social Sciences (SPSS®) for Windows® release 21.0 (SPSS Inc., Chicago, IL, USA). Results: A total of 129 (6.9%) and 105 (5.6%) mycobacteria was isolated by MGIT 960 and by LJ culture respectively among 1879 extrapulmonary specimens. MGIT 960 identified 118 (91.5%) as Mycobacterium tuberculosis complex and 11 (8.5%) as NTM among the total mycobacteria isolated. Out of 105 mycobacteria grown by LJ culture, 95 (90.5%) and 10 (9.5%) were identified as Mycobacterium tuberculosis and NTM, respectively. The rate of contamination associated with MGIT 960 and LJ culture was 4.6% and 4.3% respectively. The Time to Detection (TTD) was found to be significantly shorter for isolation of Mycobacterium tuberculosis by MGIT 960 culture compared to LJ culture. Conclusion: In the current study, authors compared MGIT 960 with solid LJ culture for recovery of both Mycobacterium tuberculosisComplex and NTM from extrapulmonary specimens and authors found increased recovery by MGIT 960 compared to LJ culture and also shorter duration of detection for Mycobacterium tuberculosis by MGIT 960 with comparable contamination rates.

scholarly journals Spoligotyping, Resistensi Mycobacterium tuberculosis terhadap obat TB dan Faktor Risiko Penularan Tuberkulosis Paru di Kota Jayapura

2019 ◽  
Vol 47 (1) ◽  
pp. 1-12
Author(s):  
Hana Krismawati ◽  
Antonius Oktavian ◽  
Lydia Chaidir ◽  
Evy Iriani Natalia ◽  
Melda Suebu ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Pulmonary Tuberculosis ◽  
Tuberculosis Patients ◽  
Tuberculosis Transmission ◽  
Mycobacterium Growth Indicator Tube ◽  
Indicator Tube ◽  
Solid Media ◽  
Mdr Tb ◽  
Close Contacts ◽  
Growth Indicator

Identification of the Mycobacterium tuberculosis strain found in pulmonary tuberculosis patients in Jayapura using Spolygotyping was carried out Patients with pulmonary tuberculosis are taken as an index case. Sputum samples were taken to be diagnosed with Acid Fast Staining and to culture M. tuberculosis bacteria using Lowensten Jansen solid media, Ogawa solid media and Mycobacterium Growth Indicator Tube liquid media. The patient's home visit was conducted to determine the environmental conditions of the patient's residence, interview the close contacts and test the bacterial infection M. tuberculosis in close contact cases using the Mantoux test. A thorax radiology of close contacts was carried out in the hospital's radiology unit. The results of spolygotyping showed 8%sputum samples M. tuberculosis of Beijing strain, while the rest are M. tuberculosis non Beijing strains. Among the 46 of Non-Beijing strains, the type U (likely S) is most transmitted by tuberculosis patients to their close contacts, which is as much as 6%. This study confirms the factors that influence tuberculosis transmission are the sleeping location of close contacts with the patients, the frequency of meetings that occur at least once or more in a day, density of house occupants and the presence of clinical symptoms of TB. Drugs Resistance Susceptibility test results showed that 12 patients were resistance to TB drugs and one of them was MDR TB from strain LAMP. Key words : tuberculosis transmission, Spolygotyping, tuberculosis, Jayapura, drugs resistance   Abstrak Identifikasi galur Mycobacterium tuberculosis yang ditemukan pada penderita tuberculosis paru di Jayapura telah dilakukan dengan menggunakan Spoligotyping Penderita tuberkulosis paru diambil sebagai kasus indeks. Sampel sputum juga diambil untuk didiagnosis dengan pengecatan Basil Tahan Asam dan kultur bakteri M.tuberculosis yang dilakukan pada media padat Lowensten Jansen, media padat Ogawa serta media cair Mycobacterium Growth Indicator Tube. Ekstraksi DNA dilakukan pada koloni Mycobacterium tuberculosis untuk dilakukan analisis spoligotyping. Kunjungan rumah pasien dilakukan untuk mengetahui kondisi lingkungan tempat tinggal pasien, mewawancara kontak kasus dan melakukan skrining infeksi bakteri M.tuberkulosis pada kasus kontak menggunakan tes Mantoux. Foto dada pada kontak kasus selanjutnya dilakukan di unit radiologi rumah sakit. Hasil spoligotyping menunjukkan 8% (4/50) sampel sputum penderita tuberkulosis adalah M.tuberculosis galur Beijing, sedangkan sisanya adalah M.tuberculosis galur Non Beijing. Dari 46 galur Non-Beijing, tipe U (likely S) paling banyak ditransmisikan oleh penderita tuberculosis pada kontaknya yaitu sebanyak 6% (3/50). Penelitian ini mengkonfirmasi faktor-faktor yang mempengaruhi transmisi tuberkulosis meliputi lokasi tidur kontak kasus yang serumah dengan penderita, frekuensi pertemuan yang terjadi sedikitnya sekali atau lebih dalam sehari, kondisi rumah padat penghuni dan adanya gejala klinis TB. Hasil uji resistensi menunjukkan 12 pasien mengalami resistensi terhadap OAT dan 1 diantaranya adalah MDR TB dari strain LAMP. Kata kunci : Transmisi, Splygotyping, Mycobacterium tuberculosis, tuberkulosis, Jayapura, resistensi

scholarly journals Accuracy of Xpert Ultra in Diagnosis of Pulmonary Tuberculosis among Children in Uganda: a Substudy from the SHINE Trial

2020 ◽  
Vol 58 (9) ◽  
Author(s):  
Willy Ssengooba ◽  
Jean de Dieu Iragena ◽  
Lydia Nakiyingi ◽  
Serestine Mujumbi ◽  
Eric Wobudeya ◽  
...  
Keyword(s):  
Positive Result ◽  
Diagnostic Challenge ◽  
Indicator Tube ◽  
Lowenstein Jensen ◽  
Mgit Culture ◽  
Mycobacterial Growth ◽  
Culture Positive ◽  
Mycobacterial Growth Indicator Tube ◽  
Higher Sensitivity ◽  
Growth Indicator

ABSTRACT Childhood tuberculosis (TB) presents significant diagnostic challenges associated with paucibacillary disease and requires a more sensitive test. We evaluated the diagnostic accuracy of Xpert MTB/RIF Ultra (Ultra) compared to other microbiological tests using respiratory samples from Ugandan children in the SHINE trial. SHINE is a randomized trial evaluating shorter treatment in 1,204 children with minimal TB disease in Africa and India. Among 352 samples and one cervical lymph node fine needle aspirate, one sample was randomly selected per patient and tested with the Xpert MTB/RIF assay (Xpert) and with Lowenstein-Jensen medium (LJ) and liquid mycobacterial growth indicator tube (MGIT) cultures. We selected only uncontaminated stored sample pellets for Ultra testing. We estimated the sensitivity of Xpert and Ultra against culture and a composite microbiological reference standard (any positive result). Of 398 children, 353 (89%) had culture, Xpert, and Ultra results. The median age was 2.8 years (interquartile range [IQR], 1.3 to 5.3); 8.5% (30/353) were HIV infected, and 54.4% (192/353) were male. Of the 353, 31 (9%) were positive by LJ and/or MGIT culture, 36 (10%) by Ultra, and 16 (5%) by Xpert. Sensitivities (95% confidence intervals [CI]) were 58% (39 to 65% [18/31]) for Ultra and 45% (27 to 64% [14/31]) for Xpert against any culture-positive result, with false positives of <1% and 5.5% for Xpert and Ultra. Against a composite microbiological reference, sensitivities were 72% (58 to 84% [36/50]) for Ultra and 32% (20 to 47% [16/50]) for Xpert. However, there were 17 samples that were positive only with Ultra (majority trace). Among children screened for minimal TB in Uganda, Ultra has higher sensitivity than Xpert. This represents an important advance for a condition which has posed a diagnostic challenge for decades.

scholarly journals Isoniazid Resistance in Mycobacterium tuberculosis Is a Heterogeneous Phenotype Composed of Overlapping MIC Distributions with Different Underlying Resistance Mechanisms

2019 ◽  
Vol 63 (7) ◽  
Author(s):  
Arash Ghodousi ◽  
Elisa Tagliani ◽  
Eranga Karunaratne ◽  
Stefan Niemann ◽  
Jennifer Perera ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Resistance Mechanisms ◽  
Whole Genome ◽  
Tube System ◽  
Isoniazid Resistance ◽  
Content Type ◽  
Indicator Tube ◽  
Promoter Mutations ◽  
Level Resistance ◽  
Growth Indicator

ABSTRACT MIC testing using the Bactec mycobacteria growth indicator tube system 960 of 70 phylogenetically diverse, isoniazid-resistant clinical strains of Mycobacterium tuberculosis revealed a complex pattern of overlapping MIC distributions. Whole-genome sequencing explained most of the levels of resistance observed. The MIC distribution of strains with only inhA promoter mutations was split by the current concentration endorsed by the Clinical and Laboratory Standards Institute to detect low-level resistance to isoniazid and is, consequently, likely not optimally set.

scholarly journals Evaluation of a manual identification system for detection of Mycobacterium tuberculosis in a primary tuberculosis laboratory in China

2019 ◽  
Vol 47 (6) ◽  
pp. 2666-2673 ◽  
Author(s):  
Ping Zhao ◽  
Qin Yu ◽  
Yu Zhang
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Diagnostic Performance ◽  
Culture Method ◽  
Cross Sectional Study ◽  
Identification System ◽  
Culture Methods ◽  
Cross Sectional ◽  
Indicator Tube ◽  
Conventional Culture ◽  
Time To Detection

Objective To compare the diagnostic performance of the manual BACTEC™ Mycobacteria Growth Indicator Tube (MGIT™) system (M-MGIT) with the automated BACTEC™ MGIT™ 960 system (A-MGIT) and Löwenstein-Jensen (L-J) culture method in detecting mycobacteria in sputum specimens from patients with suspected pulmonary tuberculosis (TB). Methods For this cross-sectional study, sputum samples were taken from patients aged ≥18 years attending a TB clinic in Beijing, China between July 2015 and October 2016. Processed sputum samples were inoculated into the MGIT systems and L-J medium for up to 6 and 8 weeks, respectively. Results The M-MGIT and A-MGIT methods detected significantly more Mycobacterium tuberculosis complex (MTC) isolates than L-J culture from the 565 sputum samples (39%, 40% and 32%, respectively). Using a positive result from any of the three culture systems as reference, the sensitivity of M-MGIT, A-MGIT and L-J methods were 92%, 94%, and 74%, respectively. The time-to-detection of mycobacteria was 12.9±4.2 days for M-MGIT, 11.8±5.2 days for A-MGIT and 24.2±8.7 days for L-J. Conclusions M-MGIT has a similar diagnostic performance to A-MGIT, and is a fast and reliable alternative to conventional culture methods in the diagnosis of pulmonary TB in a developing country.

scholarly journals Multicenter Evaluation of the BACTEC MGIT 960 System for Recovery of Mycobacteria

1999 ◽  
Vol 37 (3) ◽  
pp. 748-752 ◽  
Author(s):  
Bruce A. Hanna ◽  
Adeleh Ebrahimzadeh ◽  
L. Bruce Elliott ◽  
Margie A. Morgan ◽  
Susan M. Novak ◽  
...  
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Liquid Medium ◽  
Mycobacterium Avium ◽  
Culture Systems ◽  
Solid Media ◽  
Lowenstein Jensen ◽  
The Mean

We evaluated the BACTEC MGIT 960 system, which is a fully automated, noninvasive system for the growth and detection of mycobacteria with a capacity to incubate and continuously monitor 960 7-ml culture tubes. We studied 3,330 specimens, 2,210 respiratory and 1,120 nonrespiratory specimens, collected from 2,346 patients treated at six sites. Processed specimens were inoculated into the BACTEC MGIT 960 and BACTEC 460 TB systems, as well as onto Lowenstein-Jensen slants and Middlebrook 7H11/7H11 selective plates. From all culture systems, a total of 362 isolates of mycobacteria were recovered; these were recovered from 353 specimens collected from 247 patients. The greatest number of isolates of mycobacteria (289, or 80% of the 362 isolates) was recovered with the BACTEC MGIT 960, followed by the BACTEC 460 TB (271, or 75%) and solid media (250, or 69%). From all culture systems a total of 132 isolates of Mycobacterium tuberculosiscomplex were recovered. The greatest number of isolates of M. tuberculosis complex was recovered when liquid medium was combined with conventional solid media; the number recovered with BACTEC 460 TB plus solid media was 128 (97%), that recovered with BACTEC MGIT 960 plus solid media was 121 (92%), that recovered with BACTEC 460 TB was 119 (90%) and that recovered with all solid media combined was 105 (79%). The recovery with BACTEC MGIT 960 alone was 102 (77%). The mean times to detection (TTD) for M. tuberculosis complex were 14.4 days for BACTEC MGIT, 15.2 days for BACTEC 460 TB, and 24.1 days for solid media. The numbers of isolates of Mycobacterium avium complex (MAC) recovered were 172 (100%) for all systems, 147 (85%) for BACTEC MGIT 960, 123 (72%) for BACTEC 460 TB, and 106 (62%) for all solid media combined. The TTD for MAC in each system were 10.0 days for BACTEC MGIT 960, 10.4 days for BACTEC 460 TB, and 25.9 days for solid media. Breakthrough contamination rates (percentages of isolates) for each of the systems were 8.1% for BACTEC MGIT 960, 4.9% for BACTEC 460 TB, and 21.1% for all solid media combined.

scholarly journals Macrocolonies (Granules) Formation as a Cause of False-Negative Results in the MGIT 960 System: Cause Analysis and Correlation with Mycobacterial Species

2015 ◽  
Vol 2015 ◽  
pp. 1-5 ◽  
Author(s):  
Xia Yu ◽  
Liping Zhao ◽  
Guanglu Jiang ◽  
Yifeng Ma ◽  
Weimin Li ◽  
...  
Keyword(s):  
Bacterial Load ◽  
False Negative ◽  
Drug Susceptibility ◽  
Granule Formation ◽  
Negative Results ◽  
Indicator Tube ◽  
False Negative Results ◽  
Specific Species ◽  
Culture Positive ◽  
Growth Indicator

Background. The viable mycobacterial bacilli can sometimes form granules in the Mycobacterium Growth Indicator Tube (MGIT) to produce instrument-negative outcomes when BACTEC MGIT 960 culture is performed. The cause of this phenomenon has never been analyzed.Methods. Thirty-one instrument-negative, granule presenting MGIT vials were collected for conducting acid-fast staining and also liquid and solid subculture. Species identification and drug susceptibility test were performed with the recovered strains. Cultivation test was done by inoculating small amount of bacilli into the MGIT vials.Results. Twenty-four and twenty-nine of the tested MGIT vials were smear and culture positive, respectively. In total, 18, 4, and 7 of the cultivated strains were identified asMycobacterium tuberculosiscomplex,M. intracellulare, andM. xenopi, respectively. When a limited amount of bacilli was inoculated, the granule formation was observed forM. xenopistrains in the MGIT system.Conclusions. The granules observed in the instrument-negative MGIT vials consisted of viable bacilli, which emphasized the need of visual inspection to increase recovery rate. Limited bacterial load and specific species might be the cause of granule forming.

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