Evaluation of transgenic peanut plants encoding coat protein and nucleocapsid protein genes for resistance to tobacco streak virus and peanut bud necrosis virus

Background: Peanut stem necrosis disease (PSND) caused by Tobacco streak virus (TSV) is a major constraint for groundnut production in Andhra Pradesh (A.P.). However, studies on prevalence and spread of the disease confined to only few districts of A.P. with this background current study focused on incidence and spread of the disease in entire state of A.P. Further an isolate of TSV occurring in A.P. characterized on the basis of genetic features by comparing with other TSV isolates originated from different hosts and locations from world.Methods: Roving survey was conducted during kharif 2017-18 in groundnut growing districts of Andhra Pradesh (A.P.) for peanut stem necrosis disease incidence. Groundnut plants showing PSND symptoms were collected and tested with direct antigen coating enzyme linked immunosorbent assay (DAC-ELISA). Groundnut samples found positive by ELISA once again tested by reverse transcription polymerase chain reaction (RT-PCR). The representative TSV-GN-INDVP groundnut isolate from Prakasham district was maintained on cowpea seedlings by standard sap inoculation method in glasshouse for further molecular characterization. The Phylogenetic tree for coat protein (CP) gene was constructed using aligned sequences with 1000 bootstrap replicates following neighbor-joining phylogeny.Result: Thirty-eight (52.7%) of seventy-two groundnut samples collected from different locations in A.P were given positive reaction to TSV by DAC-ELISA. For the first time, PSND incidence observed in coastal districts (Krishna, Guntur, Sri Pottisriramulu Nellore, Prakasham) of A.P. Maximum PSND incidence recorded from Bathalapalli (22.2%) and the minimum incidence in Mulakalacheruvu (4.1%). The coat protein (CP) gene of TSV-GN-INDVP groundnut isolate was amplified by RT-PCR and it shared maximum per cent nucleotide identity (97.51-98.62%) with TSV isolates from groundnut and other different crops reported in India. All Indian isolates cluster together irrespective of crop and location based on the phylogenetic analysis.

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A serological report on groundnut bud necrosis virus (GBNV) and tobacco streak virus (TSV) infecting groundnut and parthenium in Guntur and Prakasam districts of A.P.

AGRICULTURE UPDATE ◽

10.15740/has/au/12.techsear(6)2017/1646-1651 ◽

2017 ◽

Vol 12 (Special-6) ◽

pp. 1646-1651

Author(s):

G. SOWMYA LAKSHMI ◽

V. MANOJ KUMAR

Keyword(s):

Tobacco Streak Virus ◽

Streak Virus ◽

Necrosis Virus ◽

Groundnut Bud Necrosis Virus

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Transgenic tobacco expressing tobacco streak virus or mutated alfalfa mosaic virus coat protein does not cross-protect against alfalfa mosaic virus infection

Virology ◽

10.1016/0042-6822(88)90551-x ◽

1988 ◽

Vol 164 (2) ◽

pp. 383-389 ◽

Cited By ~ 78

Author(s):

Cees M.P. van Dun ◽

Bert Overduin ◽

Lous van Vloten-Doting ◽

John F. Bol

Keyword(s):

Coat Protein ◽

Virus Infection ◽

Mosaic Virus ◽

Transgenic Tobacco ◽

Alfalfa Mosaic Virus ◽

Tobacco Streak Virus ◽

Streak Virus ◽

Virus Coat Protein ◽

Virus Coat ◽

Mosaic Virus Infection

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Standardization of duplex RT-PCR for simultaneous detection of Peanut bud necrosis virus and Tobacco streak virus naturally infecting peanut (Arachis hypogeae L.)

International Journal of Clinical and Biological Sciences ◽

10.7324/ijcbs.2017.220109 ◽

2017 ◽

Keyword(s):

Simultaneous Detection ◽

Tobacco Streak Virus ◽

Streak Virus ◽

Rt Pcr ◽

Necrosis Virus

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Nucleotide sequence of the putative recognition site for coat protein in the RN As of alfalfa mosaic virus and tobacco streak virus

Nucleic Acids Research ◽

10.1093/nar/8.15.3307 ◽

1980 ◽

Vol 8 (15) ◽

pp. 3307-3318 ◽

Cited By ~ 47

Author(s):

Ellen C. Koper-Zwarthoff ◽

John F. Bol

Keyword(s):

Nucleotide Sequence ◽

Coat Protein ◽

Mosaic Virus ◽

Alfalfa Mosaic Virus ◽

Recognition Site ◽

Tobacco Streak Virus ◽

Streak Virus

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Viral Coat Protein Peptides with Limited Sequence Homology Bind Similar Domains of Alfalfa Mosaic Virus and Tobacco Streak Virus RNAs

Journal of Virology ◽

10.1128/jvi.72.4.3227-3234.1998 ◽

1998 ◽

Vol 72 (4) ◽

pp. 3227-3234 ◽

Cited By ~ 16

Author(s):

Maud M. Swanson ◽

Patricia Ansel-McKinney ◽

Felicia Houser-Scott ◽

Vidadi Yusibov ◽

L. Sue Loesch-Fries ◽

...

Keyword(s):

Coat Protein ◽

Rna Binding ◽

Alfalfa Mosaic Virus ◽

Rna Replication ◽

Tobacco Streak Virus ◽

Streak Virus ◽

Coat Proteins ◽

Rna Fragments ◽

Viral Coat ◽

Genome Activation

ABSTRACT An unusual and distinguishing feature of alfalfa mosaic virus (AMV) and ilarviruses such as tobacco streak virus (TSV) is that the viral coat protein is required to activate the early stages of viral RNA replication, a phenomenon known as genome activation. AMV-TSV coat protein homology is limited; however, they are functionally interchangeable in activating virus replication. For example, TSV coat protein will activate AMV RNA replication and vice versa. Although AMV and TSV coat proteins have little obvious amino acid homology, we recently reported that they share an N-terminal RNA binding consensus sequence (Ansel-McKinney et al., EMBO J. 15:5077–5084, 1996). Here, we biochemically compare the binding of chemically synthesized peptides that include the consensus RNA binding sequence and lysine-rich (AMV) or arginine-rich (TSV) environment to 3′-terminal TSV and AMV RNA fragments. The arginine-rich TSV coat protein peptide binds viral RNA with lower affinity than the lysine-rich AMV coat protein peptides; however, the ribose moieties protected from hydroxyl radical attack by the two different peptides are localized in the same area of the predicted RNA structures. When included in an infectious inoculum, both AMV and TSV 3′-terminal RNA fragments inhibited AMV RNA replication, while variant RNAs unable to bind coat protein did not affect replication significantly. The data suggest that RNA binding and genome activation functions may reside in the consensus RNA binding sequence that is apparently unique to AMV and ilarvirus coat proteins.

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First Report of Tobacco streak virus Infecting Guizotia abyssinica from India

Plant Disease ◽

10.1094/pdis-91-3-0330a ◽

2007 ◽

Vol 91 (3) ◽

pp. 330-330 ◽

Cited By ~ 4

Author(s):

N. Arun Kumar ◽

M. Lakshmi Narasu ◽

U. B. Zehr ◽

K. S. Ravi

Keyword(s):

Amino Acid ◽

Coat Protein ◽

The United States ◽

Tobacco Streak Virus ◽

Streak Virus ◽

Vegetable Crops ◽

Guizotia Abyssinica ◽

Oil Seed ◽

Cp Gene ◽

Seed Crops

Guizotia abyssinica (L.f.) Cass. (niger), an important oil seed crop grown in India, is used in foods, paints, soaps, and as an illuminant. During a survey conducted in 2004 to monitor Tobacco streak virus (TSV) in Helianthus annuus L. (sunflower) and Arachis hypogaea L. (groundnut), typical symptoms of leaf and petiole necrosis were observed in niger plants from Karnataka State, India. The field-collected samples reacted with TSV-specific polyclonal antiserum in direct antigen coated (DAC)-ELISA. Indicator host species were mechanically inoculated with extracts from symptomatic leaves and grown under greenhouse conditions. The inoculations resulted in local necrotic lesions on Vigna unguiculata cv. C-152 (cowpea), Gomphrena globosa, and Nicotiana tabacum cv. Xanthi (tobacco) at 3 to 4 days postinoculation (dpi) and systemic mosaic mottling on sunflower and G. globosa at 7 to 9 dpi. To identify the virus at the molecular level, total RNA was isolated (RNeasy kit, Qiagen Inc., Chatsworth, CA) from the virus-inoculated cowpea leaf and used for reverse transcription-PCR using TSV CP (coat protein) specific primers (2). The resulting ~720-bp amplicon corresponding to the CP gene of TSV was cloned into pGem-T vector (Promega, Madison, WI) and sequenced. The resulting sequence of the TSV-niger isolate (TSV-NG) comprised 717 nucleotides encoding 238 amino acid residues of the viral coat protein (GenBank Accession No. DQ864458). Comparison of the sequence with those of other TSV CP gene indicated 98.5 to 99.3% nucleotide and 97.9 to 99.6% amino acid sequence identity with TSV isolates from India (1,2; GenBank Accession Nos. AF505073, AY061930, AY061929, AF515823, AF515824, and AF515825). The sequence of TSV-NG had 89.5 and 80.0% amino acid identity with TSV-WC, type strain from the United States (GenBank Accession No. X00435) and TSV-BR, isolate from Brazil (GenBank Accession No. AY354406), respectively. On the basis of symptoms, transmission, and serological and molecular data, the causal agent of necrosis in niger was identified as a strain of TSV widely prevalent in other oil seed and vegetable crops in India. The new report of Tobacco streak virus infecting niger from India, indicated the expansion of host range among oil seed crops. References: (1) A. I. Bhat et al. Indian J Biotechnol. 1:350, 2002. (2) K. S. Ravi et al. Plant Pathol. 50:800, 2001.

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