VALIDATION OF A SIMPLES METHOD FOR SIMULTANEOUS DETERMINATION OF LIPOIC ACID AND RESVERATROL BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

2018 ◽  
Vol 2 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Irene C K Guerreiro ◽  
Samuel Davies ◽  
Silvia Guterres ◽  
Simone Jacobus Berlitz
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Lipoic Acid ◽  
High Performance ◽  
Dosage Forms ◽  
Solid Dosage Forms ◽  
Solid Dosage ◽  
Precision And Accuracy

A high-performance liquid chromatography-ultraviolet (HPLC-UV) method was developed and validated for simultaneous determination of Resveratrol (RSV) and Lipoic Acid (LA). A C18 column was used with a mobile phase consisting of acetonitrile and 0.01M phosphoric acid (60:40). The detection wavelength was at 235 nm. The method was specific in the presence of pharmaceutical excipients widely used in solid dosage forms or lipid-core nanocapsules. The results demonstrated linearity between 5 and 50 µg/mL for RSV and 30 and 120 µg/mL for LA. The method presented precision and accuracy (RSD <5%). In addition, the developed method was considered robust. Therefore, the developed method can be applied successfully for simultaneous determination of RSV and LA in the proposed conditions, with a potential application to assay both drugs in several dosage forms.

scholarly journals High performance liquid chromatography for simultaneous determination of xipamide, triamterene and hydrochlorothiazide in bulk drug samples and dosage forms

2016 ◽  
Vol 66 (1) ◽  
pp. 109-118 ◽  
Author(s):  
Soad S. Abd El-Hay ◽  
Hisham Hashem ◽  
Ayman A. Gouda
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
High Performance ◽  
Dosage Forms ◽  
Hplc Method ◽  
Pharmaceutical Dosage Forms ◽  
Temperature Detection ◽  
Bulk Drug

Abstract A novel, simple and robust high-performance liquid chromatography (HPLC) method was developed and validated for simultaneous determination of xipamide (XIP), triamterene (TRI) and hydrochlorothiazide (HCT) in their bulk powders and dosage forms. Chromatographic separation was carried out in less than two minutes. The separation was performed on a RP C-18 stationary phase with an isocratic elution system consisting of 0.03 mol L−1 orthophosphoric acid (pH 2.3) and acetonitrile (ACN) as the mobile phase in the ratio of 50:50, at 2.0 mL min−1 flow rate at room temperature. Detection was performed at 220 nm. Validation was performed concerning system suitability, limits of detection and quantitation, accuracy, precision, linearity and robustness. Calibration curves were rectilinear over the range of 0.195–100 μg mL−1 for all the drugs studied. Recovery values were 99.9, 99.6 and 99.0 % for XIP, TRI and HCT, respectively. The method was applied to simultaneous determination of the studied analytes in their pharmaceutical dosage forms.

scholarly journals A VALIDATED REVERSED-PHASE HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY METHOD FOR SIMULTANEOUS DETERMINATION OF FIVE ANTIEPILEPTIC DRUGS USED IN THE TREATMENT OF LENNOX–GASTAUT SYNDROME IN THEIR PHARMACEUTICAL DOSAGE FORMS

2018 ◽  
Vol 11 (5) ◽  
pp. 167
Author(s):  
Sonia T Hassib ◽  
Hanaa M A Hashem ◽  
Marianne A Mahrouse ◽  
Eman A Mostafa
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Antiepileptic Drugs ◽  
High Performance ◽  
Reversed Phase ◽  
Dosage Forms ◽  
Pharmaceutical Dosage Forms ◽  
Significant Difference

 Objective: Lennox–Gastaut syndrome (LGS) is mainly treated with antiepileptic drugs (AEDs) but using one AED is not sufficient to relieve all or even most patients. A combination of agents is usually preferred. In the current study, an isocratic, selective, sensitive, precise, and accurate reversed-phase high-performance liquid chromatography (RP-HPLC) method was developed for the simultaneous determination of rufinamide (RUF), lamotrigine (LAM), clonazepam (CLO), valproic acid (VAL), and diazepam (DIA) which are commonly used in the management of LGS in their dosage forms using lacosamide as internal standard.Methods: The method depends on using RESTEK C18 column (5 μm, 250 mm × 4.6 mm) and a mobile phase composed of acetonitrile:water (55: 45, v/v), pH = 3.3 adjusted with phosphoric acid. The method was conducted in an isocratic mode with a flow rate of 1ml/min and ultraviolet detection at 210 nm.Results: The linearity range was 2–40 μg/ml for RUF and DIA, 0.5–40 μg/ml for LAM and CLO, and 36–180 μg/ml for VAL.Conclusion: Statistical analysis revealed no significant difference between the results obtained and the official or reported ones for each cited drug. The method is simple to be easily implemented in quality control studies of the mentioned drugs in their pharmaceutical preparations.

Analysis of Tetracyclines (TCs) Residues in Honey with HPLC - UV

2010 ◽  
Vol 159 ◽  
pp. 89-94
Author(s):  
Hai Ying Zhang ◽  
Dong Hui Chen ◽  
Yi Zheng ◽  
Hong Tao Hu
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Perchloric Acid ◽  
Mass Concentration ◽  
High Performance ◽  
Simple Method ◽  
Coefficient Variation ◽  
Precision And Accuracy

Simultaneous determination of oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC), and doxycycline (DC) residues in honey was performed using a high-performance liquid chromatography with ultraviolet spectrophotometer ant this method was validated by analysis of precision and accuracy. Honey was digested using perchloric acid of 2% mass concentration. Matrix matched calibration curves showed a linearity exceeding 0.99. The recovery ratios lay between 75.9% and 92.5% and the coefficient variation was lower than 16.6%. Limits of detection and quantitation were below 0.03µg mL-1 and 0.09µg mL-1, respectively, for OTC and TC, and below 0.07µg mL-1 and 0.20µg mL-1, respectively, for CTC and DC. The experiments demonstrated that it is a simple method suitable for detection of residual TCs in honey.

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