Analysis of Tetracyclines (TCs) Residues in Honey with HPLC - UV

2010 ◽  
Vol 159 ◽  
pp. 89-94
Author(s):  
Hai Ying Zhang ◽  
Dong Hui Chen ◽  
Yi Zheng ◽  
Hong Tao Hu
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Perchloric Acid ◽  
Mass Concentration ◽  
High Performance ◽  
Simple Method ◽  
Coefficient Variation ◽  
Precision And Accuracy

Simultaneous determination of oxytetracycline (OTC), tetracycline (TC), chlortetracycline (CTC), and doxycycline (DC) residues in honey was performed using a high-performance liquid chromatography with ultraviolet spectrophotometer ant this method was validated by analysis of precision and accuracy. Honey was digested using perchloric acid of 2% mass concentration. Matrix matched calibration curves showed a linearity exceeding 0.99. The recovery ratios lay between 75.9% and 92.5% and the coefficient variation was lower than 16.6%. Limits of detection and quantitation were below 0.03µg mL-1 and 0.09µg mL-1, respectively, for OTC and TC, and below 0.07µg mL-1 and 0.20µg mL-1, respectively, for CTC and DC. The experiments demonstrated that it is a simple method suitable for detection of residual TCs in honey.

VALIDATION OF A SIMPLES METHOD FOR SIMULTANEOUS DETERMINATION OF LIPOIC ACID AND RESVERATROL BY HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

2018 ◽  
Vol 2 (1) ◽  
pp. 13-20 ◽  
Author(s):  
Irene C K Guerreiro ◽  
Samuel Davies ◽  
Silvia Guterres ◽  
Simone Jacobus Berlitz
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Lipoic Acid ◽  
High Performance ◽  
Dosage Forms ◽  
Solid Dosage Forms ◽  
Solid Dosage ◽  
Precision And Accuracy

A high-performance liquid chromatography-ultraviolet (HPLC-UV) method was developed and validated for simultaneous determination of Resveratrol (RSV) and Lipoic Acid (LA). A C18 column was used with a mobile phase consisting of acetonitrile and 0.01M phosphoric acid (60:40). The detection wavelength was at 235 nm. The method was specific in the presence of pharmaceutical excipients widely used in solid dosage forms or lipid-core nanocapsules. The results demonstrated linearity between 5 and 50 µg/mL for RSV and 30 and 120 µg/mL for LA. The method presented precision and accuracy (RSD <5%). In addition, the developed method was considered robust. Therefore, the developed method can be applied successfully for simultaneous determination of RSV and LA in the proposed conditions, with a potential application to assay both drugs in several dosage forms.

scholarly journals Simultaneous Determination of Eight β-Lactam Antibiotics, Amoxicillin, Cefazolin, Cefepime, Cefotaxime, Ceftazidime, Cloxacillin, Oxacillin, and Piperacillin, in Human Plasma by Using Ultra-High-Performance Liquid Chromatography with Ultraviolet Detection

2016 ◽  
Vol 60 (8) ◽  
pp. 4734-4742 ◽  
Author(s):  
Tiphaine Legrand ◽  
Dominique Vodovar ◽  
Nicolas Tournier ◽  
Nihel Khoudour ◽  
Anne Hulin
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Human Plasma ◽  
High Performance ◽  
Internal Standard ◽  
Therapeutic Drug ◽  
Simple Method ◽  
Validation Parameters

ABSTRACTA simple and rapid ultra-high-performance liquid chromatography (UHPLC) method using UV detection was developed for the simultaneous determination of eight β-lactam antibiotics in human plasma, including four penicillins, amoxicillin (AMX), cloxacillin (CLX), oxacillin (OXA), and piperacillin (PIP), and four cephalosporins, cefazolin (CFZ), cefepime (FEP), cefotaxime (CTX), and ceftazidime (CAZ). One hundred-microliter samples were spiked with thiopental as an internal standard, and proteins were precipitated by acetonitrile containing 0.1% formic acid. Separation was achieved on a pentafluorophenyl (PFP) column with a mobile phase composed of phosphoric acid (10 mM) and acetonitrile in gradient elution mode at a flow rate of 500 μl/min. Detection was performed at 230 nm for AMX, CLX, OXA, and PIP and 260 nm for CFZ, FEP, CTX, and CAZ. The total analysis time did not exceed 13 min. The method was found to be linear at concentrations ranging from 2 to 100 mg/liter for each compound, and all validation parameters fulfilled international requirements. Between- and within-run accuracy errors ranged from −5.2% to 11.4%, and precision was lower than 14.2%. This simple method requires small-volume samples and can easily be implemented in most clinical laboratories to promote the therapeutic drug monitoring of β-lactam antibiotics. The simultaneous determination of several antibiotics considerably reduces the time to results for clinicians, which may improve treatment efficiency, especially in critically ill patients.

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