scholarly journals Morphometric characteristics of TGF-β1-positive cells of fetal rat brain in vitro

2016 ◽  
Vol 4 (2) ◽  
pp. 211-215
Author(s):  
L. Liubich ◽  
M. Lisyany ◽  
T. Malysheva ◽  
V. Semenova ◽  
L. Staino ◽  
...  
Keyword(s):  
Rat Brain ◽  
Transforming Growth Factor ◽  
Tumor Therapy ◽  
Fetal Brain ◽  
Morphometric Parameters ◽  
Cross Sectional ◽  
Fetal Rat ◽  
Tgf Β1 ◽  
Fetal Rat Brain

One of the directions of cell therapy being developed for brain gliomas is the use of the neurogenic stem and progenitor cells (NSCs/NPCs). There are data on the anti-tumor and immunomodulating properties of the NSCs/NPCs the mechanisms of which were not disclosed yet. One of the potential targets for tumor therapy is the transforming growth factor β (TGF-β1) which is thought to be one of the key molecules in the regulation of proliferation, differentiation and cell survival or apoptosis. In the view of available information about the possibility of TGF-β1 production by the mammalian multipotent NSCs/NPCs, the aim of this work was to study the TGF-β1-positive cells in the dynamics of cultivation of fetal brain neurogenic cells as a potential source of anti-tumor or immunomodulating effects of these cells.Material and methods. The fetal rat brain cells on 14th (E14) day of gestation were used as the source for cultivation in standard conditions (DМЕМ + 1 % fetal bovine serum) and studied on the 2nd and 37thday by morphometry and immunocytochemistry.Results. In the fetal rat brain cell cultures, the TGF-β1-positive cells made 22.04 ± 2.33 % and the nestin-positive cells made 49.16 ± 10.60 % of the total cells number. The morphometric parameters of TGF-β1-positive cells exceeded the corresponding values of negative cells (average values of cross-sectional areas of the cytoplasm, cross-sectional areas of the nucleus, nuclear-cytoplasmic ratio). During cultivation the relative amount of TGF-β1-positive cells was slightly decreased 15.27 ± 9.80 % (p = 0.7) and their sizes were increased. On the 37th day of cultivation the sizes of TGF-β1-positive and their nuclei were smaller in the comparison with the TGF-β1-negative cells.Conclusions. The presence of TGF-β1 expression by part of neurogenic cells of fetal rat brain (E14) in vitro was found, which persisted throughout cultivation (~5 weeks). Significant quantitative differences of morphometric parameters of TGF-β1-positive and negative cells were detected.

scholarly journals TGF-β1 EXPRESSION BY GLIOMA C6 CELLS IN VITRO

2017 ◽  
Vol 39 (4) ◽  
pp. 258-263 ◽  
Author(s):  
L D Liubich ◽  
L M Kovalevska ◽  
M I Lisyany ◽  
V M Semenova ◽  
T A Malysheva ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Rat Brain ◽  
Glioma Cells ◽  
C6 Glioma ◽  
C6 Glioma Cells ◽  
C6 Cells ◽  
Fetal Rat ◽  
Tgf Β1 ◽  
Fetal Rat Brain

The aim of the work was to study the impact of fetal rat brain cell supernatant (FRBCS) on the expression of transforming growth factor β1 (TGF-β1) and p53 in C6 cells of rat glioma in vitro. Materials and Methods: FRBCS was obtained from suspensions of fetal rat brain cells on the 14th (E14) day of gestation. C6 glioma cells were cultured for 48 h in the presence of FRBCS or FRBCS + anti-TGF-β1 monoclonal antibody. Immunocytochemical staining for TGF-β1 and p53 was performed. Results: The proportion of TGF-β1-immunopositive tumor cells in C6 glioma cultures was statistically significantly higher than in the control cell cultures of normal fetal rat brain. FRBCS reduced the proportion of TGF-β1-immunopositive tumor cells and increased the proportion of p53-immunopositive cells in C6 glioma cultures. In cells cultured with FRBCS + anti-TGF-β1 monoclonal antibody, the above effects of FRBCS were abrogated. Conclusion: The obtained results suggest that TGF-β1 seems to be responsible for decrease in TGF-β1 expression and increase in p53 expression in C6 glioma cells treated with FRBCS.

scholarly journals Nerve growth cones isolated from fetal rat brain. IV. Preparation of a membrane subfraction and identification of a membrane glycoprotein expressed on sprouting neurons.

1985 ◽  
Vol 101 (5) ◽  
pp. 1977-1989 ◽  
Author(s):  
L Ellis ◽  
I Wallis ◽  
E Abreu ◽  
K H Pfenninger
Keyword(s):  
Rat Brain ◽  
Fetal Brain ◽  
Neurite Growth ◽  
Membrane Glycoprotein ◽  
Polyacrylamide Gels ◽  
Nerve Growth ◽  
Adult Rat ◽  
Fetal Rat ◽  
Fetal Rat Brain

This study describes the preparation of a membrane subfraction from isolated nerve growth cone particles (GCPs) (see Pfenninger, K. H., L. Ellis, M. P. Johnson, L. B. Friedman, and S. Somlo, 1983, Cell, 35:573-584) and the identification in this fraction of a glycoprotein expressed during neurite growth. While approximately 40 major polypeptides are visible in Coomassie Blue-stained SDS polyacrylamide gels of pelleted (partially disrupted) GCPs, a salt-washed membrane fraction prepared from lysed, detergent-permeabilized GCPs contains only 14% of this protein and has an unusually simple polypeptide pattern of seven major bands. Monoclonal antibodies have been generated to GCP membranes isolated from fetal rat brain. These antibodies have been screened differentially with synaptosomes from adult rat brain in order to identify those which recognize antigens expressed selectively during neurite growth. One such antibody (termed 5B4) recognizes a developmentally regulated membrane glycoprotein that is enriched in GCP membranes and expressed in fetal neurons sprouting in vitro. The 5B4 antigen in fetal brain migrates in SDS polyacrylamide gels as a diffuse band of approximately 185-255 kD, is rich in sialic acid, and consists of a small family of isoelectric variants. Freezing-thawing and neuraminidase digestion result in the cleavage of the native antigen into two new species migrating diffusely around 200 and 160 kD. Prolonged neuraminidase digestion sharpens these bands at about 180 and 135 kD, respectively. In the mature brain, antibody 5B4 recognizes a sparse polypeptide migrating at approximately 140 kD. As shown in the following paper (Wallis, I., L. Ellis, K. Suh, and K. H. Pfenninger, 1985, J. Cell Biol., 101:1990-1998), the fetal antigen is specifically associated with regions of neuronal sprouting and, therefore, can be used as a molecular marker of neurite growth.

Interaction between human brain tumour biopsies and fetal rat brain tissue in vitro

1990 ◽  
Vol 81 (2) ◽  
pp. 130-140 ◽  
Author(s):  
O. Engebraaten ◽  
R. Bjerkvig ◽  
M. Lund-Johansen ◽  
K. Wester ◽  
P.-H. Pedersen ◽  
...  
Keyword(s):  
Human Brain ◽  
Rat Brain ◽  
Brain Tissue ◽  
Brain Tumour ◽  
Fetal Rat ◽  
Rat Brain Tissue ◽  
Fetal Rat Brain

INTERACTIONS BETWEEN FETAL RAT BRAIN CELLS AND MATURE BRAIN TISSUE IN VIVO AND IN VITRO

1993 ◽  
Vol 52 (3) ◽  
pp. 295 ◽  
Author(s):  
Kirsten Marienhagen ◽  
Paal-Henning Pedersen ◽  
Sverre Mork ◽  
Rolf Bierkvig
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Brain Cells ◽  
Mature Brain ◽  
Fetal Rat ◽  
Fetal Rat Brain

Interactions between fetal rat brain cells and mature brain tissue in vivo and in vitro

1994 ◽  
Vol 20 (2) ◽  
pp. 130-143 ◽  
Author(s):  
K. Marienhagen ◽  
P.-H. Pedersen ◽  
A. J. A. Terzis ◽  
O. D. Laerum ◽  
H. Arnold ◽  
...  
Keyword(s):  
Rat Brain ◽  
Brain Tissue ◽  
Brain Cells ◽  
Mature Brain ◽  
Fetal Rat ◽  
Fetal Rat Brain

scholarly journals Lack of Action of Exogenously Administered T3 on the Fetal Rat Brain Despite Expression of the Monocarboxylate Transporter 8

Endocrinology ◽  
2011 ◽  
Vol 152 (4) ◽  
pp. 1713-1721 ◽  
Author(s):  
Carmen Grijota-Martínez ◽  
Diego Díez ◽  
Gabriella Morreale de Escobar ◽  
Juan Bernal ◽  
Beatriz Morte
Keyword(s):  
Gene Expression ◽  
Cerebral Cortex ◽  
Rat Brain ◽  
Organic Anion ◽  
Fetal Brain ◽  
Monocarboxylate Transporter ◽  
Anion Transporter ◽  
Fetal Rat ◽  
Fetal Rat Brain

Abstract Mutations of the monocarboxylate transporter 8 gene (MCT8, SLC16A2) cause the Allan-Herndon-Dudley syndrome, an X-linked syndrome of severe intellectual deficit and neurological impairment. Mct8 transports thyroid hormones (T4 and T3), and the Allan-Herndon-Dudley syndrome is likely caused by lack of T3 transport to neurons during critical periods of fetal brain development. To evaluate the role of Mct8 in thyroid hormone action in the fetal brain we administered T4 or T3 to thyroidectomized pregnant dams treated with methyl-mercapto-imidazol to produce maternal and fetal hypothyroidism. Gene expression was then measured in the fetal cerebral cortex. T4 increased Camk4, Sema3c, and Slc7a3 expression, but T3 was without effect. To investigate the cause for the lack of T3 action we analyzed the expression of organic anion transport polypeptide (Oatp14, Slco1c1), a T4 transporter, and Mct8 (Slc16a2), a T4 and T3 transporter, by confocal microscopy. Both proteins were present in the brain capillaries forming the blood-brain barrier and in the epithelial cells of the choroid plexus forming the blood-cerebrospinal fluid barrier. It is concluded that T4 from the maternal compartment influences gene expression in the fetal cerebral cortex, possibly after transport via organic anion transporter polypeptide and/or Mct8, and conversion to T3 in the astrocytes. On the other hand, T3 does not reach the target neurons despite the presence of Mct8. The data indicate that T4, through local deiodination, provides most T3 in the fetal rat brain. The role of Mct8 as a T3 transporter in the fetal rat brain is therefore uncertain.

scholarly journals Dynamics of CD133+ cells in cultures of glioma c6 and fetal rat brain under the neurogenic cells supernatant influence

2015 ◽  
Vol 3 (2) ◽  
pp. 150-154 ◽  
Author(s):  
L. Liubich ◽  
M. Lisyany ◽  
V. Semenova ◽  
L. Stayno
Keyword(s):  
Stem Cells ◽  
Rat Brain ◽  
Cell Cultures ◽  
Culture Conditions ◽  
Cross Sectional ◽  
Rat Glioma ◽  
Glioma C6 ◽  
Fetal Rat ◽  
Number Of Cells ◽  
Fetal Rat Brain

Cellular and molecular similarities between brain tumor stem cells (BTSCs) and normal neurogenic stem cells (NSCs) motivate the search for new methods of treatment of malignant glioma using NSCs. CD133 molecule could be one of the most typical markers of BTSCs and considered as a target for therapy of brain tumors.The aim of this study was to evaluate the effect of rat neurogenic cells supernatant (NCsS) on the content of CD133+ cells in glioma C6 cell cultures.Materials and methods. The cells of rat brain glioma C6 were used as the source for the cultivation; for comparative assessment of tested compound impact on the intact nervous system the fetal rat brain cells on 14th (E14) day of gestation were used. The study was performed in control cultures under standard culture conditions without NCsS adding and tested cultures with adding NCsS (0.10 mg/ml of protein) for 48 hours. NCsS was received from suspensions of rat brain neurogenic cells (E14).Results. CD133-positive cells were 12.05 ± 4.77 % of the total number of cells in C6 glioma culture and 37.36 ± 12.33 % of the total number of cells in fetal rat brain culture. CD133-positive cells had a smaller size than negative cells (average values of cross-sectional area of cells and nucleus) and greater nuclear-cytoplasmic ratio. The cell and nucleus sizes of CD133-positive cells in cell cultures of fetal rat brain were twice larger than sizes of such cells in cultures of glioma C6.Under the conditions of NCsS for 48 hours the reducing in the number of CD133-positive cells in rat glioma C6 cell cultures (2.88 ± 0.41 %) and lack of such effects in cell cultures of fetal rat brain (E14) were found.Conclusion. The morphological differences of CD133-positive cells in glioma C6 cultures and in cell cultures of fetal rat brain (E14) were detected. The decrease of CD133-positive cells in glioma C6 cells culture under the influence of neurogenic cells supernatant was shown.

Sign in / Sign up

Export Citation Format

Share Document