Evidence for divergent selection and spatial differentiation in a putative zona pellucida gene is indicative of local adaptation in Pacific cod

Genetic differentiation has been observed in marine species even when no obvious barriers to gene flow exist. The study of highly differentiated outlier loci can provide information on how genetic variation might contribute to local adaptation. A locus which aligned to a predicted zona pellucida sperm-binding protein 3 gene (ZP3) in Atlantic cod was previously identified in Pacific cod populations as a high differentiation outlier. In other marine species, ZP3 is involved in reproductive isolation, local adaptation, and has neofunctionalized as an antifreeze gene, but the function of this genomic region is not understood in Pacific cod. We sequenced a 544 bp region of ZP3 in 230 Pacific cod collected from throughout their geographic range. Here we show that ZP3 haplotypes exhibit strong spatial structure and there is evidence for divergent selection at this locus in samples collected from the Bering Sea region. The potential for adaptation to different thermal regimes is particularly relevant given that Pacific cod have demonstrated high natural mortality during recent ocean warming events.

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Differences between antigenic determinants of pig and cat zona pellucida proteins

Reproduction ◽

10.1530/reprod/119.1.15 ◽

2000 ◽

pp. 15-23 ◽

Cited By ~ 15

Author(s):

K Jewgenow ◽

M Rohleder ◽

I Wegner

Keyword(s):

In Vitro Fertilization ◽

Zona Pellucida ◽

Antigenic Determinants ◽

Vitro Fertilization ◽

Contraceptive Vaccine ◽

Sperm Binding ◽

Dependent Inhibition ◽

Dose Dependent Inhibition ◽

Dose Dependent

Despite many efforts, the control of reproduction in feral cat populations is still a problem in urban regions around the world. Immunocontraception is a promising approach; thus the present study examined the suitability of the widely used pig zona pellucida proteins (pZP) for contraception in feral domestic cats. Purified zona pellucida proteins obtained from pig and cat ovaries were used to produce highly specific antisera in rabbits. Antibodies against pZP raised in rabbits or lions were not effective inhibitors of either in vitro sperm binding (cat spermatozoa to cat oocytes) or in vitro fertilization in cats, whereas antibodies against feline zona pellucida proteins (fZP) raised in rabbits showed a dose-dependent inhibition of in vitro fertilization. Immunoelectrophoresis, ELISA and immunohistology of ovaries confirmed these results, showing crossreactivity of anti-fZP sera to fZP and to a lesser extent to pZP, but no interaction of anti-pZP sera with fZP. It is concluded that cat and pig zonae pellucidae express a very small number of shared antigenic determinants, making the use of pZP vaccine in cats questionable. A contraceptive vaccine based on feline zona pellucida determinants will be a better choice for the control of reproduction in feral cats if immunogenity can be achieved.

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Faculty Opinions recommendation of Human sperm binding is mediated by the sialyl-Lewis(x) oligosaccharide on the zona pellucida.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.13396969.14765074 ◽

2011 ◽

Author(s):

Dolores Lamb ◽

Alexander W Pastuszak

Keyword(s):

Zona Pellucida ◽

Human Sperm ◽

Sialyl Lewis X ◽

Lewis X ◽

Sperm Binding ◽

Sialyl Lewis

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Transcriptome Expression of Biomineralization Genes in Littoraria flava Gastropod in Brazilian Rocky Shore Reveals Evidence of Local Adaptation

Genome Biology and Evolution ◽

10.1093/gbe/evab050 ◽

2021 ◽

Vol 13 (4) ◽

Author(s):

Camilla A Santos ◽

Gabriel G Sonoda ◽

Thainá Cortez ◽

Luiz L Coutinho ◽

Sónia C S Andrade

Keyword(s):

Local Adaptation ◽

Differentially Expressed Genes ◽

Evolutionary Biology ◽

Rocky Shore ◽

Expression Patterns ◽

Purifying Selection ◽

Marine Species ◽

Differentially Expressed ◽

Planktonic Larva ◽

Structure Changes

Abstract Understanding how selection shapes population differentiation and local adaptation in marine species remains one of the greatest challenges in the field of evolutionary biology. The selection of genes in response to environment-specific factors and microenvironmental variation often results in chaotic genetic patchiness, which is commonly observed in rocky shore organisms. To identify these genes, the expression profile of the marine gastropod Littoraria flava collected from four Southeast Brazilian locations in ten rocky shore sites was analyzed. In this first L. flava transcriptome, 250,641 unigenes were generated, and 24% returned hits after functional annotation. Independent paired comparisons between 1) transects, 2) sites within transects, and 3) sites from different transects were performed for differential expression, detecting 8,622 unique differentially expressed genes. Araçá (AR) and São João (SJ) transect comparisons showed the most divergent gene products. For local adaptation, fitness-related differentially expressed genes were chosen for selection tests. Nine and 24 genes under adaptative and purifying selection, respectively, were most related to biomineralization in AR and chaperones in SJ. The biomineralization-genes perlucin and gigasin-6 were positively selected exclusively in the site toward the open ocean in AR, with sequence variants leading to pronounced protein structure changes. Despite an intense gene flow among L. flava populations due to its planktonic larva, gene expression patterns within transects may be the result of selective pressures. Our findings represent the first step in understanding how microenvironmental genetic variation is maintained in rocky shore populations and the mechanisms underlying local adaptation in marine species.

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Sperm binding to the human zona pellucida and calcium influx in response to GnRH and progesterone

Andrologia ◽

10.1111/j.1439-0272.2002.tb02945.x ◽

2002 ◽

Vol 34 (5) ◽

pp. 301-307 ◽

Cited By ~ 5

Author(s):

P. Morales ◽

E. Pizarro ◽

M. Kong ◽

C. Pasten

Keyword(s):

Zona Pellucida ◽

Calcium Influx ◽

Sperm Binding

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Characterization of Human Sperm Binding to Homologous Recombinant Zona Pellucida Proteins

Reproductive Sciences ◽

10.1177/1933719111398146 ◽

2011 ◽

Vol 18 (9) ◽

pp. 876-885 ◽

Cited By ~ 10

Author(s):

Mayel Chirinos ◽

Cecilia Cariño ◽

María Elena González-González ◽

Ernesto Arreola ◽

Rodrigo Reveles ◽

...

Keyword(s):

Zona Pellucida ◽

Human Sperm ◽

Sperm Binding

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Sperm Binding to the Zona Pellucida, Hyaluronic Acid Binding Assay, and PICSI

Non-Invasive Sperm Selection for In Vitro Fertilization ◽

10.1007/978-1-4939-1411-1_6 ◽

2014 ◽

pp. 59-68 ◽

Cited By ~ 1

Author(s):

Sergio C. Oehninger ◽

Dirk Kotze

Keyword(s):

Hyaluronic Acid ◽

Zona Pellucida ◽

Binding Assay ◽

Sperm Binding ◽

Hyaluronic Acid Binding

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Genetic population structure and tools for the management of European sprat (Sprattus sprattus)

ICES Journal of Marine Science ◽

10.1093/icesjms/fsaa113 ◽

2020 ◽

Vol 77 (6) ◽

pp. 2134-2143

Author(s):

Niall J McKeown ◽

Piera Carpi ◽

Joana F Silva ◽

Amy J E Healey ◽

Paul W Shaw ◽

...

Keyword(s):

Local Adaptation ◽

Population Connectivity ◽

Ne Atlantic ◽

Genetic Population ◽

Sprattus Sprattus ◽

Population Average ◽

Outlier Loci ◽

Baltic Population ◽

Assisted Management ◽

Mixed Stock

Abstract This study used RAD-seq-derived SNPs to explore population connectivity, local adaptation, and individual assignment in European sprat (Sprattus sprattus) and inform the alignment of management units with biological processes. FST, clustering, and outlier analyses support a genetically cohesive population spanning the Celtic Sea-English Channel-North Sea-Kattegat (NE Atlantic) region. The lack of structure among the NE Atlantic samples indicates connectivity across current management boundaries. However, the assumption of demographic panmixia is cautioned against unless verified by a multidisciplinary approach. The data confirm high genetic divergence of a Baltic population (average FST vs. NE Atlantic samples = 0.051) with signatures compatible with local adaptation in the form of outlier loci, some of which are shown to occur within exonic regions. The outliers permit diagnostic assignment of individuals between the NE Atlantic and Baltic populations and thus represent a “reduced panel” of markers for monitoring a potential mixed stock fishery within the western Baltic. Overall, this study provides information that may help refine spatial management boundaries of sprat and resources for genetic-assisted management.

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Application of loop-mediated isothermal amplification (LAMP) for rapid detection of Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus) and haddock (Melanogrammus aeglefinus)

Molecular and Cellular Probes ◽

10.1016/j.mcp.2019.07.003 ◽

2019 ◽

Vol 47 ◽

pp. 101420

Author(s):

Yi Wang ◽

Junli Feng ◽

Xiaolan Tian

Keyword(s):

Rapid Detection ◽

Gadus Morhua ◽

Atlantic Cod ◽

Isothermal Amplification ◽

Melanogrammus Aeglefinus ◽

Pacific Cod ◽

Loop Mediated Isothermal Amplification ◽

Pacific Cod Gadus Macrocephalus ◽

Gadus Macrocephalus ◽

Haddock Melanogrammus Aeglefinus

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A single domain of the ZP2 zona pellucida protein mediates gamete recognition in mice and humans

The Journal of Cell Biology ◽

10.1083/jcb.201404025 ◽

2014 ◽

Vol 205 (6) ◽

pp. 801-809 ◽

Cited By ~ 73

Author(s):

Matteo A. Avella ◽

Boris Baibakov ◽

Jurrien Dean

Keyword(s):

Transgenic Mice ◽

Zona Pellucida ◽

Human Sperm ◽

Recombinant Peptide ◽

Genetic Ablation ◽

Sperm Binding ◽

Gamete Recognition ◽

Mammalian Fertilization ◽

Human And Mouse

The extracellular zona pellucida surrounds ovulated eggs and mediates gamete recognition that is essential for mammalian fertilization. Zonae matrices contain three (mouse) or four (human) glycoproteins (ZP1–4), but which protein binds sperm remains controversial. A defining characteristic of an essential zona ligand is sterility after genetic ablation. We have established transgenic mice expressing human ZP4 that form zonae pellucidae in the absence of mouse or human ZP2. Neither mouse nor human sperm bound to these ovulated eggs, and these female mice were sterile after in vivo insemination or natural mating. The same phenotype was observed with truncated ZP2 that lacks a restricted domain within ZP251–149. Chimeric human/mouse ZP2 isoforms expressed in transgenic mice and recombinant peptide bead assays confirmed that this region accounts for the taxon specificity observed in human–mouse gamete recognition. These observations in transgenic mice document that the ZP251–149 sperm-binding domain is necessary for human and mouse gamete recognition and penetration through the zona pellucida.

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