scholarly journals Tris(methylthio)methane Produced by Mortierella hyalina Affects Sulfur Homeostasis in Arabidopsis

2021 ◽  
Author(s):  
Yu-Heng Tseng ◽  
Stefan Bartram ◽  
Michael Reichelt ◽  
Sandra Scholz ◽  
Anja Meents ◽  
...  
Keyword(s):  
Essential Oil ◽  
Plant Growth ◽  
Sulfur Metabolism ◽  
Nmr Analysis ◽  
Sulfur Deficiency ◽  
Symbiotic Interactions ◽  
Ms Analysis ◽  
Gsh Metabolism ◽  
Microbial Volatiles ◽  
Sulfur Containing

Microbial volatiles are important factors in symbiotic interactions with plants. Mortierella hyalina is a beneficial root-colonizing fungus with a garlic-like smell, and promotes growth of Arabidopsis seedlings. GC-MS analysis of the M. hyalina headspace and NMR analysis of the extracted essential oil identified the sulfur-containing volatile tris(methylthio)methane (TMTM) as the major compound. Its incorporation in seedlings was shown by 34S labeling experiment. Under sulfur deficiency, TMTM downregulated sulfur deficiency-responsive genes, prevented glucosinolate (GSL) and glutathione (GSH) diminishment, and sustained plant growth. However, excess TMTM led to accumulation of GSH and GSL and reduced plant growth. Since TMTM is not directly incorporated into cysteine, we propose that the volatile from M. hyalina influences the plant sulfur metabolism by interfering with the GSH metabolism, and alleviates sulfur imbalances under sulfur stress.

scholarly journals Tris(methylthio)methane Produced by Mortierella hyalina Affects Sulfur Homeostasis in Arabidopsis

2021 ◽  
Author(s):  
Yu-Heng Tseng ◽  
Stefan Bartram ◽  
Sandra Scholz ◽  
Michael Reichelt ◽  
Anja Meents ◽  
...  
Keyword(s):  
Essential Oil ◽  
Plant Growth ◽  
Sulfur Metabolism ◽  
Nmr Analysis ◽  
Sulfur Deficiency ◽  
Symbiotic Interactions ◽  
Ms Analysis ◽  
Gsh Metabolism ◽  
Microbial Volatiles ◽  
Sulfur Containing

Microbial volatiles are important factors in symbiotic interactions with plants. Mortierella hyalina is a beneficial root-colonizing fungus with a garlic-like smell, and promotes growth of Arabidopsis seedlings. GC-MS analysis of the M. hyalina headspace and NMR analysis of the extracted essential oil identified the sulfur-containing volatile tris(methylthio)methane (TMTM) as the major compound. Its incorporation in seedlings was shown by 34S labeling experiment. Under sulfur deficiency, TMTM downregulated sulfur deficiency-responsive genes, prevented glucosinolate (GSL) and glutathione (GSH) diminishment, and sustained plant growth. However, excess TMTM led to accumulation of GSH and GSL and reduced plant growth. Since TMTM is not directly incorporated into cysteine, we propose that the volatile from M. hyalina influences the plant sulfur metabolism by interfering with the GSH metabolism, and alleviates sulfur imbalances under sulfur stress.

scholarly journals GC/GC-MS Analysis, Isolation and Identification of Bioactive Essential Oil Components from the Bhutanese Medicinal Plant, Pleurospermum Amabile

2013 ◽  
Vol 8 (9) ◽  
pp. 1934578X1300800 ◽  
Author(s):  
Phurpa Wangchuk ◽  
Paul A. Keller ◽  
Stephen G. Pyne ◽  
Malai Taweechotipatr ◽  
Sumalee Kamchonwongpaisan
Keyword(s):  
Antibacterial Activity ◽  
Essential Oil ◽  
Medicinal Plant ◽  
Multidrug Resistant ◽  
Methyl Eugenol ◽  
Nmr Analysis ◽  
Isolation And Identification ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Oil Components

We have hydrodistilled the essential oil (EO) from the aerial parts of the Bhutanese medicinal plant, Pleurospermum amabile using a Clevenger apparatus and evaluated this EO by GC/GC-MS and NMR analysis followed by testing for bioactivity. The GC-MS analysis identified 52 compounds with ( E)-isomyristicin as a major component (32.2%). Repeated purification yielded four compounds; ( E)-isomyristicin (1), ( E)-isoapiol (2), methyl eugenol (3) and ( E)-isoelemicin (4). Compound 2 and the mother EO showed the best antiplasmodial activity against the Plasmodium falciparum strains, TM4/8.2 (chloroquine and antifolate sensitive) and K1CB1 (multidrug resistant). They exhibited mild antibacterial activity against Bacillus subtilis. None of the test samples showed cytotoxicity.

GC-MS Analysis of Essential Oil from Lavandula angustifolia Cultivated in Garhwal Himalaya

2015 ◽  
Vol 5 (4) ◽  
pp. 268-272 ◽  
Author(s):  
Pramod Singh ◽  
Harish Andola ◽  
M.S.M. Rawat ◽  
Geeta Joshi nee Pant ◽  
J.S. Jangwan
Keyword(s):  
Essential Oil ◽  
Garhwal Himalaya ◽  
Lavandula Angustifolia ◽  
Ms Analysis

scholarly journals Optimizing hydroponic culture media and NO3−/NH4+ ratio for improving essential oil compositions of purple coneflower (Echinacea purpurea L.)

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Fatemeh Ahmadi ◽  
Abbas Samadi ◽  
Ebrahim Sepehr ◽  
Amir Rahimi ◽  
Sergey Shabala
Keyword(s):  
Particle Size ◽  
Essential Oil ◽  
Plant Growth ◽  
Plant Height ◽  
Growth Parameters ◽  
Echinacea Purpurea ◽  
Peat Moss ◽  
Root Weight ◽  
Total Biomass ◽  
Growing Media

AbstractMedicinal plants represent a valuable commodity due to beneficial effects of their natural products on human health, prompting a need for finding a way to optimize/increase their production. In this study, a novel growing media with various perlite particle size and its mixture with peat moss was tested for hydroponic-based production of Echinacea purpurea medicinal plant under greenhouse conditions. The plant growth parameters such as plant height, total fresh leave weight, fresh root weight, total biomass, total chlorophyll, leaf area, and essential oil compositions were assessed. Perlite particle size in the growing media was varied from very coarse (more than 2 mm) to very fine (less than 0.5 mm), and the ratio between perlite and peat moss varied from 50:50 v/v to 30:70 v/v. In addition, two nitrate (NO3−) to ammonium (NH4+) ratios (90:10 and 70:30) were tested for each growing media. The medium containing very fine-grade perlite and 50:50 v/v perlite to peat moss ratio was found to be most optimal and beneficial for E. purpurea performance, resulting in maximal plant height, fresh and dry weight, leaf surface area, and chlorophyll content. It was also found that an increase in NO3−/NH4+ ratio caused a significant increase in plant growth parameters and increase the plant essential oil content. The major terpene hydrocarbons found in extract of E. purpurea with the best growth parameters were germacrene D (51%), myrcene (15%), α-pinene (12%), β-caryophyllene (11%), and 1-Pentadecene (4.4%), respectively. The percentages of these terpene hydrocarbons were increased by increasing of NO3−/NH4+ ratio. It can be concluded that decreasing the perlite particle size and increasing the NO3−/NH4+ ratio increased the plant growth parameters and essential oil compositions in E. purpurea.

scholarly journals GC-MS analysis of clove (Syzygium aromaticum) bud essential oil from Java and Manado

2017 ◽  
Author(s):  
B. Amelia ◽  
E. Saepudin ◽  
A. H. Cahyana ◽  
D. U. Rahayu ◽  
A. S. Sulistyoningrum ◽  
...  
Keyword(s):  
Essential Oil ◽  
Syzygium Aromaticum ◽  
Ms Analysis

scholarly journals Mycorrhizal fungi inoculation and phosphorus fertilizer on growth, essential oil production and nutrient uptake in peppermint (Mentha piperita L.)

2012 ◽  
Vol 14 (4) ◽  
pp. 692-699 ◽  
Author(s):  
M.C. Arango ◽  
M.F. Ruscitti ◽  
M.G. Ronco ◽  
J. Beltrano
Keyword(s):  
Essential Oil ◽  
Plant Growth ◽  
Arbuscular Mycorrhizal Fungi ◽  
Mycorrhizal Fungi ◽  
Glomus Intraradices ◽  
Arbuscular Mycorrhizal ◽  
Fungal Species ◽  
Mentha Piperita ◽  
Essential Oil Yield ◽  
Essential Oil Production

This study evaluated the effects of inoculation with the arbuscular mycorrhizal fungi Glomus mosseae, Glomus intraradices A4 and Glomus intraradices B1 and two phosphorus levels (10 and 40 mg kg-1) on root colonization, plant growth, nutrient uptake and essential oil content in Mentha piperita L. The experiment was carried out in a greenhouse, in 4x2 factorial arrangement, in completely randomized design. At sixty days after transplanting, the mycorrhizal plants had significantly higher fresh matter, dry matter and leaf area compared to non-mycorrhizal plants. The inoculation increased P, K and Ca levels in the shoot which were higher under 40 mg P kg-1 of soil. Plants grown with 40 mg P kg-1 soil increased the essential oil yield per plant by about 40-50% compared to those cultivated with 10 mg P kg-1, regardless of the mycorrhizal treatment. Among the studied fungal species, inoculation with G. intraradices A4 and a high level of P significantly increased plant growth and essential oil yield, compared to the other studied mycorrhizal fungal species. In conclusion, inoculation of arbuscular mycorrhizal fungi into peppermint plants is a feasible alternative to increase the essential oil production and reduce the use of fertilizers required to obtain economic production of peppermint under phosphorus-deficient soil condition.

scholarly journals Medicinal and pesticidal potentials of the constituents of the essential oil from Adenia cissampeloides leaves

2019 ◽  
Vol 68 (1-2) ◽  
pp. 7-21
Author(s):  
Modupe Ogunlesi ◽  
Wesley Okiei ◽  
Edith Ofor ◽  
Anthony Eniola
Keyword(s):  
Essential Oil ◽  
Alternative Medicine ◽  
Essential Oils ◽  
Linolenic Acid ◽  
Hexadecanoic Acid ◽  
Ms Analysis ◽  
Hexahydrofarnesyl Acetone ◽  
Potential Applications

Adenia cissampeloides (Planch. ex Hook.) Harms is used in alternative medicine primarily for the management of hypertension and several other diseases. Isolation and GC-MS analysis of five essential oils collected fractionally and over 4 h by hydrodistillation from the dried leaves were carried out. The constituents included phytol, α-linolenic acid, n-hexadecanoic acid, hexahydrofarnesyl acetone, (13S)-8,13-epoxy-labd-14-ene, kaur-16-ene, guaiol, α-gurjunene, and α-elemene. Several bioactivities of the constituents as well as their potential applications in medicine and the pharmaceutical and pesticidal industries are discussed. Essential oils from some other plants have several constituents in common with those isolated from A. cissampeloides. The bioactivities of such plants are used to suggest other possible applications of A. cissampeloides.

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