scholarly journals Zebrafish Larvae's Response to Electricity is Mediated by Dopaminergic Agonists and Antagonists

2021 ◽  
Author(s):  
Arezoo Khalili ◽  
Ellen van Wijngaarden ◽  
Georg Zoidl ◽  
Pouya Rezai
Keyword(s):  
Dopamine Agonists ◽  
Molecular Mechanisms ◽  
Screening Method ◽  
Dopamine Antagonists ◽  
Electric Response ◽  
Dopaminergic Agonists ◽  
Chemical Screening ◽  
Zebrafish Larvae ◽  
Skf 81297 ◽  
Selective Dopamine

The signaling molecular mechanisms in zebrafish response to electricity are unknown, so here we asked if changes to dopaminergic signaling pathways can affect their electrically-evoked locomotion. To answer this question, the effects of multiple selective and non-selective dopamine compounds on the electric response of zebrafish larvae is investigated. A microfluidic device with enhanced control of experimentation with multiple larvae is used, which features a novel design to immobilize four zebrafish larvae in parallel and expose them to electric current that induces tail locomotion. In 6 days post-fertilization zebrafish larvae, the electric induced locomotor response is quantified in terms of the tail movement duration and beating frequency to discern the effect of non-lethal concentrations of dopaminergic agonists (apomorphine, SKF-81297, and quinpirole), and antagonists (butaclamol, SCH-23390, and haloperidol). All dopamine antagonists decrease locomotor activity, while dopamine agonists do not induce similar behaviours in larvae. The D2- like selective dopamine agonist quinpirole enhances movement. However, exposure to non-selective and D1-selective dopamine agonists apomorphine and SKF-81297 cause no significant change in the electric response. Exposing larvae that were pre-treated with butaclamol and haloperidol to apomorphine and quinpirole, respectively, restores electric locomotion. The results demonstrate a correlation between electric response and the dopamine signalling pathway. We propose that the electrofluidic assay has profound application potential as a chemical screening method when investigating biological pathways, behaviors, and brain disorders.

scholarly journals The incentive amplifying effects of nicotine are reduced by selective and non-selective dopamine antagonists in rats

2014 ◽  
Vol 126 ◽  
pp. 50-62 ◽  
Author(s):  
Matthew I. Palmatier ◽  
Marissa R. Kellicut ◽  
A. Brianna Sheppard ◽  
Russell W. Brown ◽  
Donita L. Robinson
Keyword(s):  
Dopamine Antagonists ◽  
Selective Dopamine

scholarly journals Effects of dopamine and selective dopamine agonists upon platelet accumulation in the cerebral and pulmonary vasculature of the rabbit

1997 ◽  
Vol 122 (4) ◽  
pp. 682-686 ◽  
Author(s):  
Michael Emerson ◽  
William Paul ◽  
Pierpaolo Ferlenga ◽  
Claudio Semeraro ◽  
Clive Page
Keyword(s):  
Dopamine Agonists ◽  
Pulmonary Vasculature ◽  
Platelet Accumulation ◽  
Selective Dopamine

scholarly journals Clinical status and behavior of patients with hyperprolactinemic hypogonadism treated by selected dopamine agonists

2001 ◽  
Vol 47 (1) ◽  
pp. 11-15
Author(s):  
G. A. Melnichenko ◽  
A. Ye. Bobrov ◽  
T. I. Romantsova ◽  
M. G. Pavlova ◽  
L. S. Samsonova ◽  
...  
Keyword(s):  
Mental Status ◽  
Prolactin Level ◽  
Dopamine Agonists ◽  
Clinical Status ◽  
Somatic Symptoms ◽  
Sexual Disorders ◽  
Psychological Pressure ◽  
And Behavior ◽  
Anxiety Depression ◽  
Selective Dopamine

Mental status and behavior were studied in 19 women aged 18-45 years with hyperprolactinemic hygonadism of different forms treated by selective dopamine receptor agonists (4 patients were treated with chinagolide and 15 with cabergolide). Appreciable improvement of the general clinical status was attained: biphasic menstrual cycle was restored, galactorrhea decreased, and prolactin level normalized. Sexual disorders and headaches reduced. Mental status improved: anxiety, depression, and severity of neurovegetative symptoms decreased. Hamilton score (anxiety) decreased from 16.5 to 9.1. Versatile personality investigation showed de creased profiles of the first, third, seventh, and eighth scales, which indicates decreased affective strain, reduction of psychological partitioning and sense of one's own inadequacy, and decreased liability to exert psychological pressure on other people by means of somatic symptoms available. However, these positive shifts in the mental status of patients were insufficiently complete and stable, despite the continuing decrease in prolactin level.

scholarly journals Protease Inhibitor-Dependent Inhibition of Light-Induced Stomatal Opening

2021 ◽  
Vol 12 ◽  
Author(s):  
Tenghua Wang ◽  
Wenxiu Ye ◽  
Yin Wang ◽  
Maoxing Zhang ◽  
Yusuke Aihara ◽  
...  
Keyword(s):  
Matrix Metalloproteinase ◽  
Protease Inhibitor ◽  
Signaling Pathway ◽  
Stomatal Closure ◽  
Screening Method ◽  
Stomatal Opening ◽  
Matrix Metalloproteinase 2 ◽  
Aba Signaling ◽  
Bioinformatics Analyses ◽  
Chemical Screening

Stomata in the epidermis of plants play essential roles in the regulation of photosynthesis and transpiration. Stomata open in response to blue light (BL) by phosphorylation-dependent activation of the plasma membrane (PM) H+-ATPase in guard cells. Under water stress, the plant hormone abscisic acid (ABA) promotes stomatal closure via the ABA-signaling pathway to reduce water loss. We established a chemical screening method to identify compounds that affect stomatal movements in Commelina benghalensis. We performed chemical screening using a protease inhibitor (PI) library of 130 inhibitors to identify inhibitors of stomatal movement. We discovered 17 PIs that inhibited light-induced stomatal opening by more than 50%. Further analysis of the top three inhibitors (PI1, PI2, and PI3; inhibitors of ubiquitin-specific protease 1, membrane type-1 matrix metalloproteinase, and matrix metalloproteinase-2, respectively) revealed that these inhibitors suppressed BL-induced phosphorylation of the PM H+-ATPase but had no effect on the activity of phototropins or ABA-dependent responses. The results suggest that these PIs suppress BL-induced stomatal opening at least in part by inhibiting PM H+-ATPase activity but not the ABA-signaling pathway. The targets of PI1, PI2, and PI3 were predicted by bioinformatics analyses, which provided insight into factors involved in BL-induced stomatal opening.

Phenotypic chemical and mutant screening of zebrafish larvae using an on-demand response to electric stimulation

2019 ◽  
Vol 11 (10) ◽  
pp. 373-383 ◽  
Author(s):  
Arezoo Khalili ◽  
Amir Reza Peimani ◽  
Nickie Safarian ◽  
Khaled Youssef ◽  
Georg Zoidl ◽  
...  
Keyword(s):  
Electric Stimulation ◽  
Gene Knockout ◽  
Beat Frequency ◽  
Movement Behavior ◽  
Model Organisms ◽  
Phenotypic Characterization ◽  
Electric Response ◽  
Zebrafish Larvae ◽  
On Demand ◽  
6 Hydroxydopamine

Abstract Behavioral responses of zebrafish larvae to environmental cues are important functional readouts that should be evoked on-demand and studied phenotypically in behavioral, genetical and developmental investigations. Very recently, it was shown that zebrafish larvae execute a voluntary and oriented movement toward the positive electrode of an electric field along a microchannel. Phenotypic characterization of this response was not feasible due to larva’s rapid movement along the channel. To overcome this challenge, a microfluidic device was introduced to partially immobilize the larva’s head while leaving its mid-body and tail unrestrained in a chamber to image motor behaviors in response to electric stimulation, hence achieving quantitative phenotyping of the electrically evoked movement in zebrafish larvae. The effect of electric current on the tail-beat frequency and response duration of 5–7 days postfertilization zebrafish larvae was studied. Investigations were also performed on zebrafish exposed to neurotoxin 6-hydroxydopamine and larvae carrying a pannexin1a (panx1a) gene knockout, as a proof of principle applications to demonstrate on-demand movement behavior screening in chemical and mutant assays. We demonstrated for the first time that 6-hydroxydopamine leads to electric response impairment, levodopa treatment rescues the response and panx1a is involved in the electrically evoked movement of zebrafish larvae. We envision that our technique is broadly applicable as a screening tool to quantitatively examine zebrafish larvae’s movements in response to physical and chemical stimulations in investigations of Parkinson’s and other neurodegenerative diseases, and as a tool to combine recent advances in genome engineering of model organisms to uncover the biology of electric response.

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