Morphology, distribution, and histochemistry of trichomes of Thymus lykae Degen & Jav. (Lamiaceae)

Micromorphology, distribution, and histochemistry of the trichomes of Thymus lykae were studied using scanning electron microscopy (SEM)and confocal laser scanning electron microscopy (CLSM). The leaves, stem, and calyx bear numerous glandular and non-glandular trichomes. Two types of glandular trichomes are identified - peltate and capitate. Results of histochemical tests showed positive reactions to polysaccharides, proteins, and lipids. Yellow autofluorescence of secreted material was noticed in peltate and capitate trichomes. Strong reddish-yellow autofluorescence of the lipophilic and hydrophilic secreted material was observed with CLSM.

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The micromorphological, histochemical and confocal analysis of satureja subspicata Bartl. ex vis. glandular trichomes

Archives of Biological Sciences ◽

10.2298/abs1004143m ◽

2010 ◽

Vol 62 (4) ◽

pp. 1143-1149 ◽

Cited By ~ 9

Author(s):

Marija Marin ◽

N. Jasnic ◽

D. Lakusic ◽

Sonja Duletic-Lausevic ◽

Lia Ascensao

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Light Microscopy ◽

Positive Reaction ◽

Laser Scanning ◽

Glandular Trichomes ◽

Confocal Laser Scanning ◽

Confocal Laser ◽

Histochemical Tests ◽

Scanning Electron

Micromorphology, histochemical and confocal analyses of the trichomes of Satureja subspicata (Bartl. ex Vis.) were carried out using light microscopy, confocal laser scanning electron microscopy (CLSM), and scanning electron microscopy. Non-glandular unbranched and two types of glandular trichomes - peltate and capitate - are described. The results of histochemical tests showed a positive reaction to phenolics, tannins, lipids, acid lipids, pectins and polysaccharides in both types of glandular trichomes. A strong red autofluorescence of the lipophilic and hydrophilic secreted material in glandular trichomes was observed with CLSM.

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Microscopic investigation of trichomes of wild-growing Satureja montana L.

Archives of Biological Sciences ◽

10.2298/abs1204599m ◽

2012 ◽

Vol 64 (4) ◽

pp. 1599-1605 ◽

Cited By ~ 2

Author(s):

Marija Marin ◽

Lia Ascensão ◽

P.D. Marin

Keyword(s):

Electron Microscopy ◽

Scanning Electron Microscopy ◽

Laser Scanning ◽

Glandular Trichomes ◽

Microscopic Investigation ◽

Fluorescence Microscope ◽

Confocal Laser ◽

Capitate Trichomes ◽

Scanning Electron ◽

Satureja Montana

Considering the importance of S. montana L. as a medicinal plant, due to the essential oils produced in its glandular trichomes, and the lack of data about the leaf ultrastructure and micromorphology, we have carried out a comprehensive study of trichomes distributed in the vegetative and reproductive organs. Micromorphological and ultrastructural investigations of the glandular trichomes of Satureja montana L. and autofluorescence analyses of their secreted products were carried out using scanning electron microscopy (SEM), transmission electron microscopy (TEM), confocal laser scanning electron microscopy (CLSM) and light fluorescence microscope. The leaves, stem and calyx bear numerous nonglandular, unbranched trichomes and two types of glandular trichomes - peltate and capitate. Ultrastructural analyses showed the presence of mitochondria, plastids and proliferations of the granular endoplasmatic reticulum in secretory phases of the heads of glandular trichomes. Strong red autofluorescence of the lipophilic and hydrophilic secreted material was observed with CLSM. Green-yellow autofluorescence of secreted material were noticed in peltate and capitate trichomes observed with a fluorescence microscope.

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