scholarly journals Direct PCR amplification of the HVSI region in mitochondrial DNA from buccal cell swabs

2012 ◽  
Vol 64 (3) ◽  
pp. 851-858 ◽  
Author(s):  
Natasa Kovacevic-Grujicic ◽  
S. Davidovic ◽  
Dijana Takic ◽  
Marija Mojsin ◽  
Milena Stevanovic
Keyword(s):  
Mitochondrial Dna ◽  
Dna Analysis ◽  
Pcr Amplification ◽  
Buccal Cell ◽  
Buccal Cells ◽  
Direct Pcr ◽  
Long Term Storage ◽  
Short Period ◽  
Term Storage

Amplification of human mitochondrial DNA (mtDNA) has been widely used in population genetics, human evolutionary and molecular anthropology studies. mtDNA hypervariable segments I and II (HVSI and HVSII) were shown to be a suitable tool in genetic analyses due to the unique properties of mtDNA, such as the lack of recombination, maternal mode of inheritance, rapid evolutionary rate and high population-specific polymorphisms. Here we present a rapid and low-cost method for direct PCR amplification of a 330 bp fragment of HVSI from buccal cell samples. Avoiding the DNA isolation step makes this method appropriate for the analysis of a large number of samples in a short period of time. Since the transportation of samples and fieldwork conditions can affect the quality of samples and subsequent DNA analysis, we tested the effects of long-term storage of buccal cell swabs on the suitability of such samples for direct PCR amplification. We efficiently amplified a 330 bp fragment of HVSI even after the long-term storage of buccal cells at room temperature, +4?C or at -20?C, for up to eight months. All examined PCR products were successfully sequenced, regardless of sample storage time and conditions. Our results suggest that the direct PCR amplification of the HVSI region from buccal cells is a method well suited for large-scale mtDNA population studies.

scholarly journals Buccal Dna Collection Using Treated Paper

2020 ◽  
Author(s):  
S. Atavliyeva ◽  
P. Tarlykov
Keyword(s):  
Bacterial Growth ◽  
Tandem Repeat ◽  
Buccal Epithelium ◽  
Buccal Cells ◽  
Hypervariable Segment ◽  
Long Term Storage ◽  
Dna Collection ◽  
Term Storage ◽  
Short Tandem

This study presents a domestic kit suitable for the collection of human buccal cells, and long-term storage of nucleic acids on a piece of paper. A filter paper with a density of 70 g/m2 was selected and treated with chemicals that make up the lysing solution, which contributes to the destruction of cells and prevents bacterial growth. A total of 25 buccal cell samples were collected on the treated paper cards. The samples had a median DNA concentration of 20 ng/μl (range 0.5–45 ng/μl). The collected DNA samples were tested using a real-time PCR, sequencing of the first hypervariable segment of the human mtDNA control region, and multiplex genotyping of 15 short tandem repeat loci. We conclude that the domestic pretreated cards could be used for collecting and isolating DNA from buccal epithelium for genetic and other applications.

scholarly journals Long-term Storage and Recovery of Buccal Cell DNA from Treated Cards

2006 ◽  
Vol 15 (2) ◽  
pp. 385-388 ◽  
Author(s):  
Alice J. Sigurdson ◽  
Mina Ha ◽  
Mark Cosentino ◽  
Tracie Franklin ◽  
Kashif A. Haque ◽  
...  
Keyword(s):  
Buccal Cell ◽  
Long Term Storage ◽  
Term Storage

scholarly journals Long-term storage of samples for flow cytometric DNA analysis

Cytometry ◽  
1983 ◽  
Vol 3 (5) ◽  
pp. 317-322 ◽  
Author(s):  
Lars L. Vindeløv ◽  
Ib J. Christensen ◽  
Niels Keiding ◽  
Mogens Spang-Thomsen ◽  
Nis I. Nissen
Keyword(s):  
Dna Analysis ◽  
Long Term Storage ◽  
Flow Cytometric ◽  
Term Storage

scholarly journals Evaluation of long-term storage effects on buccal cell DNA from untreated cards for STR profiling

2020 ◽  
Vol 596 ◽  
pp. 012009
Author(s):  
Hashom Mohd Hakim ◽  
Japareng Lalung ◽  
Hussein Omar Khan ◽  
Siti Afifah Ismail ◽  
Mohd Yusmaidie Aziz ◽  
...  
Keyword(s):  
Buccal Cell ◽  
Str Profiling ◽  
Long Term Storage ◽  
Storage Effects ◽  
Term Storage

Studies of long-term storage of high quality raw sugar

2010 ◽  
pp. 419-426
Author(s):  
Vadim Kochergin
Keyword(s):  
Sugar Content ◽  
Storage Period ◽  
Future Research ◽  
High Quality ◽  
The Core ◽  
Long Term Storage ◽  
Short Period ◽  
Raw Sugar ◽  
Term Storage

Although existing contracts do not provide sufficient economic justification to increase raw sugar quality, the trend of manufacturing better quality raw sugar in the factories will likely continue. Relatively few studies have been conducted on storage of VHP (very high pol) and VLC (very low color) sugars, especially when the storage period approaches 9–10 months. Monitoring of commercial sugar shipments indicated that, after an initial relatively safe period of storage, sugar color might double or even triple in a short period of time. Large experimental piles of VHP and VLC sugar were monitored in two sugar factories with different crystallization sequences (conventional and double magma) for two consecutive seasons. Temperature and relative humidity probes were placed up to 15 m inside the piles. Samples were taken periodically to evaluate the effects of storage conditions on color, purity, invert sugar content and other parameters. It was concluded that sugar of high quality stores better compared to conventional sugar. However, even VHP sugar can double its color during long-term storage. Sampling near the surface of the sugar pile (up to 1.5 m inside the pile) is not representative of the bulk of sugar. It has been found that sugar temperature follows the ambient trend as deep as 3 m inside the pile. Sugar within 1.5 m of the surface that was not subjected to temperature increase stored well compared to sugar in the core of the pile. It is unclear what conditions trigger color increase in storage. Changes in temperature profiles of raw sugar during storage in commercial warehouses indicate that some exothermic reactions take place in the core of the piles that result in color increase and reduction of sucrose content. The reactions take place even when the initial sugar water content and temperature meet the requirements accepted for safe storage (safety factor below 0.25 and temperature below 30 °C). Lower sugar pH value may be one of the reasons that make sugar less stable in storage. Options of cooling sugar before or during the storage will be considered in future research.

Modelling of Moisture Movement in Wood during Outdoor Storage

2001 ◽  
Vol 6 (2) ◽  
pp. 3-14 ◽  
Author(s):  
R. Baronas ◽  
F. Ivanauskas ◽  
I. Juodeikienė ◽  
A. Kajalavičius
Keyword(s):  
Experimental Data ◽  
Finite Difference ◽  
Climatic Conditions ◽  
Two Dimensional ◽  
Moisture Movement ◽  
Finite Difference Technique ◽  
Long Term Storage ◽  
Space Formulation ◽  
Term Storage

A model of moisture movement in wood is presented in this paper in a two-dimensional-in-space formulation. The finite-difference technique has been used in order to obtain the solution of the problem. The model was applied to predict the moisture content in sawn boards from pine during long term storage under outdoor climatic conditions. The satisfactory agreement between the numerical solution and experimental data was obtained.

Trehalose: a cryoprotectant that enhances recovery and preserves function of human pancreatic islets after long-term storage

Diabetes ◽  
1997 ◽  
Vol 46 (3) ◽  
pp. 519-523 ◽  
Author(s):  
G. M. Beattie ◽  
J. H. Crowe ◽  
A. D. Lopez ◽  
V. Cirulli ◽  
C. Ricordi ◽  
...  
Keyword(s):  
Pancreatic Islets ◽  
Human Pancreatic Islets ◽  
Long Term Storage ◽  
Term Storage
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