scholarly journals Analysis of mitochondrial DNA in honey bees (Apis mellifera) from Serbia

2007 ◽  
Vol 57 (5-6) ◽  
pp. 465-476 ◽  
Author(s):  
Kozmus P. ◽  
Stevanovic Jevrosima ◽  
Stanimirovic Z. ◽  
Stojic V. ◽  
Kulisic Z. ◽  
...  
2018 ◽  
Vol 62 (2) ◽  
pp. 223-232
Author(s):  
Dylan Cleary ◽  
Allen L. Szalanski ◽  
Clinton Trammel ◽  
Mary-Kate Williams ◽  
Amber Tripodi ◽  
...  

Abstract A study was conducted on the mitochondrial DNA genetic diversity of feral colonies and swarms of Apis mellifera from ten counties in Utah by sequencing the intergenic region of the cytochrome oxidase (COI-COII) gene region. A total of 20 haplotypes were found from 174 honey bee colony samples collected from 2008 to 2017. Samples belonged to the A (African) (48%); C (Eastern Europe) (43%); M (Western Europe) (4%); and O (Oriental) lineages (5%). Ten African A lineage haplotypes were observed with two unique to Utah among A lineage haplotypes recorded in the US. Haplotypes belonging to the A lineage were observed from six Utah counties located in the southern portion of the State, from elevations as high as 1357 m. All five C lineage haplotypes that were found have been observed from queen breeders in the US. Three haplotypes of the M lineage (n=7) and two of the O lineage (n=9) were also observed. This study provides evidence that honey bees of African descent are both common and diverse in wild populations of honey bees in southern Utah. The high levels of genetic diversity of A lineage honey bee colonies in Utah provide evidence that the lineage may have been established in Utah before the introduction of A lineage honey bees from Brazil to Texas in 1990.


2009 ◽  
Vol 47 (9-10) ◽  
pp. 717-721 ◽  
Author(s):  
Fulya Özdil ◽  
Bahman Fakhri ◽  
Hasan Meydan ◽  
Mehmet Ali Yıldız ◽  
H. Glenn Hall

2021 ◽  
Vol 18 (1-2) ◽  
pp. 22-28
Author(s):  
O. V. Cherevatov ◽  
E. O. Melnik ◽  
R. A. Volkov

Aim. The rapidly evolving mitochondrial CoI gene is widely used in the molecular taxonomy of insects to identify closely related forms. Accordingly, to assess the distribution of subspecies / breeds of Apis mellifera in Ukraine, sequencing and comparison of this gene was performed for bees from different geographical regions. Methods. PCR amplification and sequencing of CoI. Results. Breed-specific mutations in the CoI gene have been identified for the Dark European, Carpathian and Ukrainian Steppe honey bees, which are widely distributed in Ukraine. It was found that the current distribution of these breeds does not correspond to the traditional zoning. Conclusions. The widespread practice of importing the genetic material of Apis mellifera from different regions of Ukraine leads to uncontrolled hybridization and represents a threat to the conservation of aboriginal breeds of honey bees.Keywords: biodiversity, mitochondrial DNA, molecular markers, cytochrome oxidase, Apis mellifera.


Insects ◽  
2021 ◽  
Vol 12 (7) ◽  
pp. 620
Author(s):  
Valerio Joe Utzeri ◽  
Anisa Ribani ◽  
Valeria Taurisano ◽  
Carles Hernández i Banqué ◽  
Luca Fontanesi

Growing interest has been emerging on the need to monitor the genetic integrity of the European Apis mellifera subspecies that could be threatened by the human-mediated dispersion of non-native populations and lines. Mitochondrial DNA (mtDNA) lineages can provide useful information for this purpose. In this study, we took advantage of the environmental DNA (eDNA) contained in the honey, which can be analyzed to detect the main groups of mitotypes of the honey bees that produced it. In this study, we applied this eDNA to produce a distribution map all over the Italian peninsula and the two major islands (Sicily and Sardinia) of the following three honey bee mtDNA lineages: A, C and M. A total of 607 georeferenced honey samples, produced in all Italian regions, was analyzed to detect these lineages. The A lineage was widespread in Sicily, as expected, considering that A. m. siciliana carries the African lineage. Surprisingly, this lineage was also reported in about 14% of all other samples produced in almost all continental regions, and in Sardinia. The applied method obtained an updated distribution map of honey bee mtDNA lineages that could be useful to design policies for the conservation of Italian honey bee genetic resources.


Apidologie ◽  
2015 ◽  
Vol 47 (5) ◽  
pp. 679-687 ◽  
Author(s):  
Allen L. Szalanski ◽  
Amber D. Tripodi ◽  
Clinton E. Trammel ◽  
Danielle Downey

2013 ◽  
pp. 59-63
Author(s):  
Erika Zakar ◽  
Edit Zajácz ◽  
Tímea Rácz ◽  
János Oláh ◽  
András Jávor ◽  
...  

The honey bees are essential for the pollination of agricultural plants. The Pannonian honey bee, Apis mellifera pannonica, is native to Hungary, only these subspecies are being bread in our country. The parameters have been separated the pannon and italian honey bee subspecies, the colour of tergit, the cubital index and proboscis length. The morphometric analisys is of special importance because this, on the one hand, shows correlation with honey bee production and on the other hand, the pure morphometric charactersitics is the basis of any potential honey bee export. Mitochondrial DNA and microsatellites are the common methods to define genetic diversity and the separation of subspecies.


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