scholarly journals Polymorphism of COI gene in honey bees from different regions of Ukraine

2021 ◽  
Vol 18 (1-2) ◽  
pp. 22-28
Author(s):  
O. V. Cherevatov ◽  
E. O. Melnik ◽  
R. A. Volkov
Keyword(s):  
Mitochondrial Dna ◽  
Apis Mellifera ◽  
Honey Bees ◽  
Molecular Taxonomy ◽  
Genetic Material ◽  
Pcr Amplification ◽  
Coi Gene ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Geographical Regions

Aim. The rapidly evolving mitochondrial CoI gene is widely used in the molecular taxonomy of insects to identify closely related forms. Accordingly, to assess the distribution of subspecies / breeds of Apis mellifera in Ukraine, sequencing and comparison of this gene was performed for bees from different geographical regions. Methods. PCR amplification and sequencing of CoI. Results. Breed-specific mutations in the CoI gene have been identified for the Dark European, Carpathian and Ukrainian Steppe honey bees, which are widely distributed in Ukraine. It was found that the current distribution of these breeds does not correspond to the traditional zoning. Conclusions. The widespread practice of importing the genetic material of Apis mellifera from different regions of Ukraine leads to uncontrolled hybridization and represents a threat to the conservation of aboriginal breeds of honey bees.Keywords: biodiversity, mitochondrial DNA, molecular markers, cytochrome oxidase, Apis mellifera.

Polymorphism of the СоІІ gene of honey bees in the western regions of Ukraine

2020 ◽  
Vol 12 (2) ◽  
pp. 174-179
Author(s):  
Oleksandr Cherevatov ◽  
Nadiia Roshka
Keyword(s):  
Molecular Markers ◽  
Apis Mellifera ◽  
Cytochrome Oxidase ◽  
Honey Bees ◽  
Molecular Taxonomy ◽  
Genetic Material ◽  
Gene Encoding ◽  
Geographical Regions ◽  
Coii Gene ◽  
Mitochondrial Cytochrome Oxidase

Due to active human intervention in natural genetic and population processes, survival and distribution areas of honey bees have been negatively affected. Preservation of the gene pool of aboriginal bees that are well adapted to local environmental conditions is an urgent problem that cannot be solved without the use of molecular methods to monitor the genetic composition of local populations. This type of research requires the use of state-of-the-art approaches based on application of molecular markers. In the molecular taxonomy of insects, mitochondrial cytochrome oxidase genes are widely used to identify closely related forms. The peculiarity of mtDNA is that it is maternally inherited, and the mitochondrial molecular markers are not separated by recombination. The СоІІ gene encoding the second subunit of cytochrome oxidase is widely used to descriminate the subspecies of honey bee. Therefore, to assess the distribution of different subspecies / breeds of Apis mellifera in Ukraine, the 5′-region of the СоІІ gene was sequenced and compared for bees from different geographical regions. In the CoII gene, base substitutions were found, which makes it possible to distinguish between honey bees of the Carpathian and Ukrainian steppe breeds, which are widespread in Ukraine. It has been revealed that the distribution of these breeds does not always correspond to the official zoning. The widespread practice in Ukraine of transporting the genetic material of Apis mellifera from different regions leads to uncontrolled hybridization and poses a threat to the preservation of aboriginal breeds of honey bees.

scholarly journals Pelacakan Gen Sitokrom Oksidase Sub Unit I (COI) DNA Mitokondria Itik Tegal (Anas domesticus) Menggunakan Primer Universal

2013 ◽  
Vol 15 (1) ◽  
pp. 20 ◽  
Author(s):  
Sarwo Edi Wibowo ◽  
Muhammad Anwar Djaelani ◽  
Hermin Pancasakti Kusumaningrum
Keyword(s):  
Mitochondrial Dna ◽  
Native Species ◽  
Egg Production ◽  
Genetic Characterization ◽  
Coi Gene ◽  
Universal Primers ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Gene Data ◽  
Pcr Conditions

Currently local ducks are generally quite difficult to find in a big farm in Inonesia, including Tegal ducks. Tegal ducks is one of the genetic resources native to Indonesia with it’s advantages in terms of high and large egg production. Conservation and development of local ducks have strived to maintain our existence of Indonesian livestock germplasm. If such information is not superior to native species exist, the opportunity to increase his lead further also getting smaller. Tracking the mitochondrial COI gene DNA of Tegal ducks may underlie the process of an organism's genetic characterization. Information about Tegal duck mitochondrial DNA has not been done. The information obtained can be used for optimization of duck products native to Indonesia both in physiological aspects, phylogeny and genetic engineering. The research method used in this research is tracking COI gene data from Gen Bank with the programs Clustal X and Genedoc. Tracking then continued using universal primers HCO and LCO. The results of the data followed up with the isolation and amplification of COI gene mitochondrial DNA as well as the optimization of PCR conditions. The results showed mitochondrial DNA COI gene Tegal ducks were amplified with primer LCO obtain DNA fragments of length less than 250 bp. Kata kunci: duck’s from Tegal, COI gene, mitochondrial DNA

Pilumnus Leach (Decapoda:Pilumnidae Samouelle) from Mediterranean and adjacent Atlantic waters: a COI analysis

2010 ◽  
Vol 24 (2) ◽  
pp. 182 ◽  
Author(s):  
Ulla Oliveira-Biener ◽  
Roland R. Melzer ◽  
Michael A. Miller
Keyword(s):  
Atlantic Ocean ◽  
Mediterranean Sea ◽  
Base Pair ◽  
Molecular Taxonomy ◽  
Morphological Characters ◽  
The Other ◽  
Coi Gene ◽  
Mediterranean Species ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene

A molecular taxonomy is presented based on 544 base pair sequences of the mitochondrial COI gene (Palumbi segment) from 141 bristle crabs of the genus Pilumnus from the Mediterranean Sea and adjacent Atlantic Ocean, plus outgroups. The sample includes all currently valid autochthonous Mediterranean species. Six well-supported COI lineages were detected, three of which exclusively comprise representatives of Pilumnus inermis, P. spinifer and P. villosissimus, respectively. On the other hand, crabs that would be attributed to P. hirtellus according to current morphology-based taxonomy were distributed over three COI clades, two of which are interpreted as representing cryptic lineages. We compare our findings to previous analyses using sets of external morphological characters, and discuss the partial mismatch between the morphology- and gene-based classifications.

Deep divergence and evidence for translocations between Iranian and European populations of the alfalfa weevil (Coleoptera: Curculionidae) based on mitochondrial DNA

2016 ◽  
Vol 148 (6) ◽  
pp. 703-715 ◽  
Author(s):  
E. Sanaei ◽  
M. Seiedy ◽  
J. Skuhrovec ◽  
M.A. Mazur ◽  
Ł. Kajtoch ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Coi Gene ◽  
Invasive Pest ◽  
Alfalfa Weevil ◽  
Hypera Postica ◽  
Genetic Lineages ◽  
Mitochondrial Coi ◽  
Operational Taxonomic Units ◽  
Mitochondrial Coi Gene ◽  
Holarctic Region

AbstractThe alfalfa weevil (Hypera postica(Gyllenhal): Coleoptera: Curculionidae) is an invasive pest of alfalfa (Medicago sativaLinnaeus; Fabaceae) in the Holarctic region. Across the wide geographic distribution of the species different local adaptions have been observed. Further, several distinct mitochondrial lineages have been discovered, which have been treated as western and Egyptian/eastern strains. However, our knowledge of the biogeography ofH. posticais largely limited to North American and Japanese populations. We sampled the species from four locations in Iran and two countries in Europe (Poland and Czech Republic) and sequenced the mitochondrial COI gene to detect patterns of mitochondrial DNA divergence among Iranian and European strains. We detected two groups separated by a deep molecular split, even justifying the distinction of two molecular operational taxonomic units. Joint analyses with previously published sequences suggest that the European samples may represent the western strain, whereas both the western and Egyptian/eastern strains can be found in Iran. This distribution of genetic lineages may be the result of human-mediated translocations. The directionality, however, cannot be inferred. Our data suggest that translocations may have led to the coexistence of multipleH. posticalineages in some parts of the world,e.g., Iran. We discuss these findings against the background of the current taxonomy ofH. postica.

HIGHLY COMMERCIAL FISHERIES TAWAR FISH: MOLECULAR ANALYSIS DNA MITOCHONDRIAL COI GENE SEQUENCE AND PROXIMATE ANALYSIS FROM MALACCA STRAIT, RIAU

2016 ◽  
Vol 78 (4-2) ◽  
Author(s):  
Delianis Pringgenis ◽  
Ragil Susilowati
Keyword(s):  
Local Community ◽  
Sequence Similarity ◽  
Economic Value ◽  
Pcr Amplification ◽  
Proximate Analysis ◽  
Coi Gene ◽  
Swim Bladder ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Malacca Strait

Tawar fish is one of fish that live at the Coast of Malacca strait, Riau. Even though they live at sea but the local community has mentioned it as “tawar” fish, which means freshwater fish. Tawar fish is not found in the other place. The most interesting thing of this fish is their dried swim bladder that have an expensive price can reach as much as IDR 15 000 000                 (± USD 1 200) per kg. The local community usually sell them directly to Singapore. The myth has found that this swim bladder is useful for health and could recover diseases although the actual benefit for health has not been well explored. The aim of this research was to investigate molecular approach with PCR amplification COI gene (Cytochrome Oxidase subunit I) and by means proximate analysis on the content muscle and swim bladder. The result showed that tawar fish (FBR_BCL with accession number LC064301) have 92 % sequence similarity with Boesemania microlepsis. While through the proximate analysis, it was found that this species had high protein mainly in swim bladder as much as 36.21 %. Boesemania microlepsis (Bleeker, 1858) have an economic value.

A review of the genus Forficuloecus Conci (Phthiraptera: Philopteridae) from parrots (Psittaciformes: Psittacidae), with descriptions of four new species

Zootaxa ◽  
2008 ◽  
Vol 1859 (1) ◽  
pp. 49 ◽  
Author(s):  
ROGER D. PRICE ◽  
KEVIN P. JOHNSON ◽  
RICARDO L. PALMA
Keyword(s):  
New Species ◽  
Coi Gene ◽  
Chewing Louse ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
Genetic Distinctiveness

Ten species, including four new species, of the chewing louse genus Forficuloecus Conci, 1941, are recognized from Australasian parrots, and a key is given for their identification. The new species and their type hosts are: F. cameroni ex the Red-winged Parrot, Aprosmictus erythropterus (J.F. Gmelin, 1788); F. banksi ex the Mulga Parrot, Psephotus varius Clark, 1910; F. wilsoni ex the Northern Rosella, Platycercus venustus (Kuhl, 1820); and F. josephi ex the Bourke's Parrot, Neopsephotus bourkii (Gould, 1841). Partial sequences of the mitochondrial COI gene support the genetic distinctiveness of these new species.

Are Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara (Acari: Tetranychidae) one species? Morphological and molecular evidences for synonymy between these two spider mite species and a note on invasiveness of T. macfarlanei on okra and eggplant in India

2017 ◽  
Vol 22 (4) ◽  
pp. 467
Author(s):  
Mahran Zeity ◽  
Nagappa Srinivas ◽  
Chinnamade Channegowde Gowda
Keyword(s):  
Spider Mite ◽  
Morphological Characters ◽  
Molecular Data ◽  
Mite Species ◽  
Coi Gene ◽  
High Similarity ◽  
Mitochondrial Coi ◽  
Mitochondrial Coi Gene ◽  
High Degree ◽  
Degree Of Similarity

Study of morphological characters of Tetranychus macfarlanei Baker & Pritchard and Tetranychus malaysiensis Ehara revealed high similarity by comparing all the important characters in addition to the characters pointed out by Ehara to separate those two species. Molecular phylogeny of seven Indian populations of T. macfarlanei and one population of T. malaysiensis from Philippines along with few distantly related species of Tetranychus was attempted. High degree of similarity between these two species at mitochondrial COI gene (96%) as well as ITS2 (rDNA) (96–99%) region was evident. Based on both morphological features and molecular data, T. malaysiensis is proposed as a junior synonym of T. macfarlanei based on ICZN’s law of priority. Also more female characters are prompted in this study to distinctly discriminate T. macfarlanei from its most resembling species, Tetranychus ludeni Zacher. Tetranychus macfarlanei has emerged as a pest of several cultivated crop plants in India. 

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