scholarly journals The biotechnological production of xanthan on vegetable oil industry wastewaters. Part I: Modelling and optimization

2017 ◽  
Vol 23 (3) ◽  
pp. 329-339 ◽  
Author(s):  
Bojana Bajic ◽  
Damjan Vucurovic ◽  
Sinisa Dodic ◽  
Zorana Roncevic ◽  
Jovana Grahovac ◽  
...  
Keyword(s):  
Carbon Source ◽  
Vegetable Oil ◽  
Xanthomonas Campestris ◽  
Phosphorus Content ◽  
Oil Industry ◽  
Raw Material ◽  
Nitrogen And Phosphorus ◽  
Biotechnological Production ◽  
Xanthan Production ◽  
Industry Wastewater

The aim of this research was to examine the possibility of using the vegetable oil industry wastewater as the basis of the cultivation media for xanthan production, using Xanthomonas campestris ATCC 13951, in order to reuse the wastewater obtained in one industry as a raw material in another. Furthermore, the aim was to optimize the composition of wastewater based media in terms of carbon, nitrogen, and phosphorus content. Experiments were carried out in accordance with the Box-Behnken design with three factors on three levels (glucose: 10.00- -30.00 g/L; nitrogen: 0.02-0.20 g/L; phosphorus: 0.0045-0.045 g/L) and three repetitions at the central point. The success of biosynthesis was assessed by analysing broths to determine xanthan yield, cultivation broth apparent viscosity, residual carbon, nitrogen, and phosphorus content. Graphical and numerical optimization using response surface methodology was performed to determine the optimal content values of the carbon source, nitrogen, and phosphorus content. The results of graphical optimization suggest that for the defined response interval values, the defined model predicts that the xanthan production is possible when 15.00-16.00 g/L of the carbon source, 0.02-0.09 g/L of nitrogen and 0.01-0.02 g/L of phosphorus are added to the media that as a basis contains vegetable oil industry wastewater.

scholarly journals The biotechnological production of xanthan on vegetable oil industry wastewaters (part II): Kinetic modelling and process simulation

2018 ◽  
Vol 24 (2) ◽  
pp. 127-137
Author(s):  
Bojana Bajic ◽  
Damjan Vucurovic ◽  
Sinisa Dodic ◽  
Zorana Roncevic ◽  
Jovana Grahovac ◽  
...  
Keyword(s):  
Carbon Source ◽  
Vegetable Oil ◽  
Process Simulation ◽  
Kinetic Modelling ◽  
Oil Industry ◽  
Simulation Software ◽  
Production Facility ◽  
Wide Range ◽  
Xanthan Production ◽  
Kinetics Of

Xanthan is a microbial biopolymer with a wide range of industrial applications and it is expected that the demand for this product will significantly increase in the coming decade and for this reason it is important to constantly work on improving all aspects of this biotechnological process. The aim of this research was to examine the kinetics of batch cultivation of Xanthomonas campestris ATCC 13951 using vegetable oil industry wastewaters as a basis for the cultivation medium, in order to produce the biopolymer xanthan. Kinetic modelling is very important for process control, reducing process costs and increasing product quality. By performing xanthan production on a medium with optimized content, the experimental values of content of biomass, carbon source and the desired product were obtained and used to determine the kinetics of biosynthesis. In order to describe biomass multiplication, product formation and carbon source consumption, the logistics, the Luedeking-Piret and modified Luedeking- -Piret equation, respectively, were successfully used. Additionally, using process simulation software (SuperPro Designer?), a process and cost model for a xanthan production facility was developed. The developed model represents the basis for a 21,294.29 and 23,107.97 kg/year xanthan production facility, which uses a vegetable oil industry wastewater-based medium and a semi-synthetic medium. The simulation model of the suggested xanthan production process, developed and based on defined kinetic models, represents an excellent basis for its further improvement and for increasing its efficiency.

scholarly journals Potential of different Xanthomonas campestris strains for xanthan biosynthesis on waste glycerol from biodiesel production

2020 ◽  
Vol 24 (2) ◽  
pp. 62-66
Author(s):  
Zorana Rončević ◽  
Ida Zahović ◽  
Nikolina Danilović ◽  
Siniša Dodić ◽  
Jovana Grahovac ◽  
...  
Keyword(s):  
Xanthomonas Campestris ◽  
Crude Glycerol ◽  
Rapid Expansion ◽  
Raw Material ◽  
Biodiesel Production ◽  
Nitrogen And Phosphorus ◽  
Sustainable Solutions ◽  
Waste Glycerol ◽  
Xanthan Production ◽  
The Media

A rapid expansion of the biodiesel industry has created various ecological issues relative to crude glycerol disposal. Xanthan biosynthesis is considered one of the sustainable solutions for minimizing the adverse effects of waste crude glycerol on the environment. The initial phase of xanthan production on crude glycerol entails the screening of producing microorganism. Therefore, the purpose of this study is to examine the possibility of xanthan production on a crude glycerol-based medium using different Xanthomonas campestris strains. The bioprocesses performed were assessed according to the rheology of the media considered, amounts of xanthan produced and conversion degrees of the most important nutrients present. The pseudoplastic behavior of all the media considered, the amounts of xanthan produced (5.22-7.67 g/L) and the degrees of crude glycerol, total nitrogen and phosphorus conversion (34.44-57.61 %, 23.04-30.35 % and 18.20-22.28 %, respectively) suggest that crude glycerol, after additional bioprocess optimization, can be a suitable raw material for the industrial production of xanthan.

scholarly journals Capacity of Histoplasma capsulatum to Survive the Composting Process

2019 ◽  
Vol 2019 ◽  
pp. 1-9
Author(s):  
Luisa Fernanda Gómez Londoño ◽  
Laura Carolina Pérez León ◽  
Juan Guillermo McEwen Ochoa ◽  
Alejandra Zuluaga Rodriguez ◽  
Carlos Alberto Peláez Jaramillo ◽  
...  
Keyword(s):  
Nested Pcr ◽  
Phosphorus Content ◽  
Histoplasma Capsulatum ◽  
Organic Fertilizer ◽  
Raw Material ◽  
Chicken Manure ◽  
High Nitrogen ◽  
Dimorphic Fungus ◽  
Nitrogen And Phosphorus ◽  
Composting Process

Histoplasma capsulatum (H. capsulatum) is a thermal-dimorphic fungus, the causal agent of histoplasmosis. Its presence in the environment is related with chicken manure due to their high nitrogen and phosphorus content. In Colombia, chicken manure is the most used raw material in the composting process; however, there is no information about the capacity of H. capsulatum to survive and remain viable in a composted organic fertilizer. To address this question, this study shows three assays based on microbiological culture and the Hc100 nested PCR. First, a composting reactor system was designed to transform organic material under laboratory conditions, and the raw material was inoculated with the fungus. From these reactors, the fungus was not isolated, but its DNA was detected. In the second assay, samples from factories where the DNA of the fungus was previously detected by PCR were analyzed. In the raw material samples, 3 colonies of H. capsulatum were isolated and its DNA was detected. However, after the composting process, neither the fungus was recovered by culture nor DNA was detected. In the third assay, sterilized and nonsterilized organic composted samples were inoculated with H. capsulatum and then evaluated monthly during a year. In both types of samples, the fungus DNA was detected by Hc100 nested PCR during the whole year, but the fungus only grew from sterile samples during the first two months evaluated. In general, the results of the assays showed that H. capsulatum is not able to survive a well-done composting process.

scholarly journals Xanthan production on wastewaters from wine industry

2017 ◽  
Vol 71 (2) ◽  
pp. 145-153 ◽  
Author(s):  
Zorana Roncevic ◽  
Bojana Bajic ◽  
Damjan Vucurovic ◽  
Sinisa Dodic ◽  
Jovana Grahovac ◽  
...  
Keyword(s):  
Xanthomonas Campestris ◽  
Sugar Content ◽  
Wine Industry ◽  
Raw Material ◽  
Experimental Conditions ◽  
Wine Production ◽  
Xanthan Production ◽  
Valuable Product ◽  
Rosé Wine ◽  
Sugar Conversion

Wine industry generates large volumes of wastewaters resulting from numerous cleaning operations that occur during the different stages of winemaking. Disposal of these effluents in the environment causes huge problems due to their high organic and inorganic load and seasonal variability. The bioconversion of winery wastewaters in valuable product, such as xanthan, is an important alternative to overcome environmental problems. In this research, the possibility of xanthan production using Xanthomonas campestris on blended wastewaters from different stages of white and rose wine production with initial sugar content of 50 g/L was investigated. In addition to the media parameters (content of sugars, total and assimilable nitrogen, phosphorus, total dissolved salts and apparent viscosity), raw xanthan yield and degree of sugar conversion into product were determined in order to examine the success of xanthan biosynthesis. In applied experimental conditions, xanthan yield of 20.92 and 30.64 g/L and sugar conversion into product of 40.23 and 60.73% were achieved on wastewaters from white and rose wine production, respectively. The results of these experiments suggest that winery wastewaters, after additional optimization of the process in terms of the substrate composition and the cultivation conditions, may be a suitable raw material for industrial xanthan production.

scholarly journals Effect of the initial glycerol concentration in the medium on the xanthan biosynthesis

2014 ◽  
pp. 239-246 ◽  
Author(s):  
Zorana Roncevic ◽  
Bojana Bajic ◽  
Jovana Grahovac ◽  
Sinisa Dodic ◽  
Jelena Dodic
Keyword(s):  
Xanthomonas Campestris ◽  
Average Molecular Weight ◽  
Process Efficiency ◽  
Glycerol Concentration ◽  
Agitation Rate ◽  
Nitrogen And Phosphorus ◽  
Experimental Conditions ◽  
Batch Mode ◽  
Residual Content ◽  
Xanthan Production

This study is concerned with the effect of different initial glycerol concentrations in the medium on xanthan production by Xanthomonas campestris ATCC 13951. Xanthan biosynthesis was carried out in batch mode under aerobic conditions at a temperature of 30oC and agitation rate of 150 rpm for 7 days. The process efficiency was estimated based on the values of raw xanthan yield, average molecular weight of the polymer and residual content of glycerol, total nitrogen and phosphorus. Based on these results, the initial concentration of glycerol as a carbon source in the production medium was suggested. In the applied experimental conditions, high raw xanthan yield (12.15 g/l) of good quality (Mw = 2.86?105 g/mol) and the lowest amount of residual nutrients (glycerol 2.75 g/l, nitrogen 0.46 g/l and phosphorus 0.67 g/l) was achieved in the medium with the initial glycerol content of 20 g/l. The obtained results are the basis for optimization of xanthan production on glycerol containing media in order to increase the product yield and quality.

Sign in / Sign up

Export Citation Format

Share Document