Antioxidant properties of hot water extracts from carpophore and spores of mushroom Ganoderma lucidum

Ganoderma lucidum (Leyss.:Fr.) Karst is one of the medicinal mushrooms, which possesses enviable antioxidant properties. Objective of this investigation was to evaluate antioxidant activity, reducing power, scavenging abilities on 1.1-diphenyl-2-picrylhydrazyl (DPPH) radicals and chelating effects on ferrous ions of hot water extracts obtained from carpophore and spores of this mushroom. Hot water extract from carpophore (Gl-I) showed high antioxidant activity of 85.7 ? 0.7%, at 10 mg/ml, while antioxidant activity of hot water extract from spores (Gl-Is) was 9.2 ? 0.3% at 10 mg/ml. Reducing power of Gl-I reached a plateau of 3.4 ? 0.1 at 20 mg/ml, and 0.3 ? 0.0 at 20 mg/ml for Gl-Is. At 10 mg/ml, scavenging ability on DPPH radicals of Gl-I increased to 96.8 ? 2.5%, whereas Gl-Is scavenged DPPH radicals by 69.6 ? 2.5% at 10 mg/ml. Gl-I chelated 81.6 ? 3.6 % of ferrous ions at 20 mg/ml, while the chelating effect of Gl-Is was 73.8 ? 1.7%. The antioxidative activities of hot water extracts from carpophore and spores of the mushroom G. lucidum were concentration dependent and increased with an increase in the concentration.

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Antioxidant Properties of Glossogyne tenuifolia

The American Journal of Chinese Medicine ◽

10.1142/s0192415x06004223 ◽

2006 ◽

Vol 34 (04) ◽

pp. 707-720 ◽

Cited By ~ 17

Author(s):

Joan-Hwa Yang ◽

Shu-Yao Tsai ◽

Ching-Mei Han ◽

Chun-Chien Shih ◽

Jeng-Leun Mau

Keyword(s):

Cold Water ◽

Antioxidant Properties ◽

Water Extract ◽

Hot Water ◽

Butylated Hydroxyanisole ◽

Ferrous Ions ◽

Water Extracts ◽

Hot Water Extract ◽

Ethanolic Extracts ◽

Glossogyne Tenuifolia

Glossogyne tenuifolia (Labill) Cass. (Compositae) is a special medicinal plant in the Pescadores Islands. Ethanolic, cold and hot water extracts were prepared from the dried herb and their antioxidant properties and components were studied. Ascorbic acid, α-tocopherol, butylated hydroxyanisole, citric and ethylenediaminetetraacetic acids were used in assays for comparison. With regard to EC50 values in antioxidant activity, ethanolic and hot water extracts (0.08 and 0.09 mg/ml) were much more effective than the cold water extract (0.76 mg/ml). At 1.0 mg/ml, reducing capacities were 1.57, 0.31 and 1.04 for ethanolic, cold water and hot water extracts, respectively. Scavenging abilities on 1,1-diphenyl-2-picrylhydrazyl radicals were in descending order: ethanolic > cold water > hot water extracts. At 20 mg/ml, the hot water extract chelated all hydroxyl ions (100%) whereas the scavenging ability of the cold water extract was 68.86%. Chelating abilities on ferrous ions were in descending order: cold water > hot water > ethanolic extracts. Phenols were found to be the major antioxidant components. All EC50 values were below 20 mg/ml, and some even below 0.1 mg/ml, indicating that all three extracts from G. tenuifolia were rich in antioxidant properties.

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Antioxidant Properties of Various Extracts from Laoying Tea (Litsea coreana L.)

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.183-185.45 ◽

2011 ◽

Vol 183-185 ◽

pp. 45-49

Author(s):

Hong Fang Ji ◽

Ling Wen Zhang ◽

Hai Yan Zhang ◽

Ming Duo Yang ◽

Jian Li

Keyword(s):

Cold Water ◽

Southern China ◽

Antioxidant Properties ◽

Water Extract ◽

Reducing Power ◽

Hot Water ◽

Ferrous Ions ◽

Water Extracts ◽

Naturally Occurring ◽

Chelating Ability

Laoying Tea, leaves of Litsea coreana L., is conventionally consumed as healthy tea beverage in southern China for hundreds of years. The ethanolic, cold water and hot water extracts were prepared and their antioxidant properties studied. At 160 µg/mL, the cold water extract showed a high reducing power of 1.056. With regard to the scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radicals, extracts were effective in the order: ethanolic > cold water > hot water extracts. EC50values for chelating ability on ferrous ions for ethanolic, cold water and hot water extracts were 10.23, 0.85 and 1.12 mg/mL, respectively. Total phenols were the major naturally occurring antioxidant components found and were in the order: cold water > hot water > ethanolic extracts. Based on EC50values, the various extracts from laoying tea were effective antioxidants.

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Antioxidant Properties of Extracts from Chroogomphis rutillus

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.183-185.40 ◽

2011 ◽

Vol 183-185 ◽

pp. 40-44 ◽

Cited By ~ 1

Author(s):

Hong Fang Ji ◽

Ling Wen Zhang ◽

Hai Yan Zhang ◽

Ming Duo Yang ◽

Yuan Zhang

Keyword(s):

Cold Water ◽

Antioxidant Properties ◽

Reducing Power ◽

Ethanolic Extract ◽

Edible Mushroom ◽

Hot Water ◽

Ferrous Ions ◽

Water Extracts ◽

Chelating Ability

Chroogomphis rutillus is an ectomycorrhizal edible mushroom available in China. The ethanolic, cold water and hot water extracts were prepared and their antioxidant properties studied. At 4 mg/mL, the ethanolic extract showed a high reducing power of 1.561, whereas those of cold water and hot water extracts were 1.161 and 0.808. With regard to the scavenging ability on 1,1-diphenyl-2-picrylhydrazyl radicals, extracts were effective in the order: ethanolic > cold water > hot water extracts. EC50values for chelating ability on ferrous ions for ethanolic, cold water and hot water extracts were 15.755, 0.059, and 0.067 mg/mL, respectively. Based on EC50values, the various extracts from C. rutillus were effective antioxidants.

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Extract from wild strain of mushroom Ganoderma lucidum as natural antioxidant

Zbornik Matice srpske za prirodne nauke ◽

10.2298/zmspn1120289k ◽

2011 ◽

pp. 289-297

Author(s):

Maja Kozarski ◽

Anita Klaus ◽

Miomir Niksic

Keyword(s):

Ascorbic Acid ◽

Ganoderma Lucidum ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Wild Strain ◽

Radical Scavenging ◽

Reducing Power ◽

Hot Water ◽

Ferrous Ions ◽

Chelating Ability

Recently, much attention has been paid to revealing natural biomaterials for clinical purposes since use of synthetic antioxidants is restricted due to their carcinogenicity. Among various natural antioxidants, polysaccharides, in general, have strong antioxidant activities and can be explored as novel potential antioxidants. The aim of this work was to examine the antioxidant properties of hot water extracted polysaccharides from Ganoderma lucidum in the form of mature fruit bodies, collected from the Bojcinska forest near Belgrade, the Republic of Serbia. Antioxidant properties were assayed in vitro, by the conjugated diene method, reducing power, scavenging abilities against 1.1-diphenyl-2-picrylhydrazyl (DPPH) radicals and chelating ability on ferrous ions. At concentrations of 1 mg/ml, the scavenging ability of G. lucidum polysaccharide extract on DPPH radicals was 74.7 %. At 1 mg/ml, the radical scavenging ability of the positive controls BHT, ascorbic acid and ?-tocopherol were 11.5, 77.1 and 79.4 %, respectively. The antioxidant activity of the polysaccharide extract increased as the concentration increased to 78.0% at 20 mg/ml. Antioxidant activities of ascorbic acid and ?-tocopherol were 63.8 % and 65.4% at 20 mg/ml. Polysaccharide extract from G. lucidum showed steady increase in the reducing activity as concentrations increased to 2.9 at 20 mg/ml. Ascorbic acid, used as a positive control, had a reducing power of 3.9 at 5 mg/ml. Chelating effects of the polysaccharide extract on ferrous ion increased with the increased concentrations. At 0.1-20 mg/ml, the chelating ability of G. lucidum polysaccharide extract was between 10.3-87.8%. The chelating effect of the synthetic metal chelator EDTA was 100% at 0.1-20 mg/ml, while citric acid did not prove to be good chelating agent for ferrous ions in this assay since its chelating ability was 10.3% at 20 mg/ml.

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Antioxidant Properties of Hot Water Extracts from Armillaria Mellea and Hohenbuehelia Serotina

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.343-344.463 ◽

2011 ◽

Vol 343-344 ◽

pp. 463-468

Author(s):

Hong Fang Ji ◽

Ling Wen Zhang ◽

Hai Yan Zhang ◽

Hai Juan Nan ◽

Da Guang Yang ◽

...

Keyword(s):

Radical Scavenging Activity ◽

Antioxidant Properties ◽

Radical Scavenging ◽

Reducing Power ◽

Hot Water ◽

Ferrous Ions ◽

Water Extracts ◽

Armillaria Mellea ◽

Dpph Radical Scavenging ◽

Better Than

Hot water extracts from Armillaria mellea and Hohenbuehelia serotina fruit bodies were prepared and investigated for their antioxidant capacity in three different assays, namely, 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, reducing power and chelating ability on ferrous ions. Among the two mushrooms hot water extracts, extract from A. mellea was better than that from H. serotina in scavenging ability and chelating effect on ferrous ions. With regard to reducing power, EC50 value of extracts from H. serotina was 0.447 mg/mL, while that of extracts from A. mellea was 1.914. From EC50 values obtained, it can be concluded that hot water extracts from A. mellea and H. serotina were good in antioxidant properties.

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Antioxidant Activities of Extracts from Pholiota Nameko

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.343-344.457 ◽

2011 ◽

Vol 343-344 ◽

pp. 457-462

Author(s):

Hong Fang Ji ◽

Ling Wen Zhang ◽

Hai Yan Zhang ◽

Guang Lei Li ◽

Ming Duo Yang

Keyword(s):

Cold Water ◽

Antioxidant Activities ◽

Antioxidant Properties ◽

Water Extract ◽

Reducing Power ◽

Edible Mushroom ◽

Hot Water ◽

Ferrous Ions ◽

Ethanol Extracts ◽

Northeast Of China

Holiota nameko is an edible mushroom widely cultivated in the northeast of China. The cold water, hot water and ethanol extracts were prepared and their antioxidant properties were studied. At 2 mg/mL, the cold water extract showed a high reducing power of 1.502, whereas those of hot water and ethanol extracts were 0.868 and 0.159. With regard to the scavenging ability on DPPH free radicals, extracts were effective in the order: cold water > hot water > ethanol extracts. EC50 values for chelating ability on ferrous ions for cold water, hot water, and ethanol extracts were 1.461, 0.663, and 11.791 mg/mL, respectively. Based on EC50 values, the water extracts of P. nameko were effective antioxidants.

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An insight into in vitro antioxidant activity of Cantharellus cibarius hot water extract for the potential application in meat products

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/333/1/012089 ◽

2019 ◽

Vol 333 ◽

pp. 012089

Author(s):

S Novakovic ◽

I Djekic ◽

J Vunduk ◽

A Klaus ◽

JM Lorenzo ◽

...

Keyword(s):

Antioxidant Activity ◽

Potential Application ◽

Meat Products ◽

Water Extract ◽

Hot Water ◽

Hot Water Extract ◽

Insight Into ◽

In Vitro Antioxidant Activity

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Antioxidant Activity and Total Phenolic Content in Shiitake Mycelial Exudates

Natural Product Communications ◽

10.1177/1934578x1100600622 ◽

2011 ◽

Vol 6 (6) ◽

pp. 1934578X1100600 ◽

Cited By ~ 1

Author(s):

Weihuan Huang ◽

Joo-Shin Kim ◽

Hau Yin Chung

Keyword(s):

Antioxidant Activity ◽

Total Phenolic Content ◽

Phenolic Content ◽

Antioxidant Properties ◽

Reducing Power ◽

In Vitro Assays ◽

Total Phenolic ◽

Shiitake Mushroom ◽

Ferrous Ions

Exudates (DE) secreted from two shiitake mushroom mycelia (strains 1358 and L5458) were evaluated for their antioxidative properties and phenolic content. 1358DE and L5458DE showed distinct antioxidant activity in different in vitro assays, including scavenging activity on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals, hydroxyl radical, superoxide anions and hydrogen peroxide; the ability to chelate ferrous ions; reducing power; hemolysis inhibition activity in rat erythrocyte; and lipid peroxidation inhibition (IC50 values of 1358DE and L5458DE were 3.3 and 132.6; 44.5 and > 1000; 26.9 and 53.7; 153.6 and >175.0; 176.0 and 521.0; 26.7 and 746.4; 47.8 and 736.9; and 3.1 and > 1000 μg/mL, respectively). Their total phenolic content was 237.33 and 24.08 mg gallic acid equivalent (GAE)/g of dry DE, respectively. Overall, these results show that 1358DE generally possesses better antioxidant properties than L5458DE, possibly due to its larger total phenolic content. Shiitake mushroom mycelial exudates, particularly of 1358DE, could be a good source of natural antioxidants.

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