Shared Epitope for Monoclonal IR162 between Anisakis simplex Larvae and Clonorchis sinensis and Cross-Reactivity between Antigens

2000 ◽  
Vol 86 (5) ◽  
pp. 1145 ◽  
Author(s):  
Jun kyong Cho ◽  
Sung.-Weon. Cho
Keyword(s):  
Shared Epitope ◽  
Clonorchis Sinensis ◽  
Cross Reactivity ◽  
Anisakis Simplex

Cross-reactivity between Anisakis simplex sensitization and visceral larva migrans by Toxocara canis

Acta Tropica ◽  
2003 ◽  
Vol 89 (1) ◽  
pp. 85-89 ◽  
Author(s):  
M.J. Perteguer ◽  
C. Cuéllar ◽  
J.L. Guillén ◽  
C. Águila ◽  
S. Fenoy ◽  
...  
Keyword(s):  
Toxocara Canis ◽  
Cross Reactivity ◽  
Anisakis Simplex ◽  
Larva Migrans ◽  
Visceral Larva Migrans

Immunological cross-reactivity analysis on recombinant histamine-releasing factors from Schistosoma japonicum, Clonorchis sinensis, and Wistar rat

2006 ◽  
Vol 100 (4) ◽  
pp. 749-754 ◽  
Author(s):  
Xiaoxiang Chen ◽  
Xuchu Hu ◽  
Zhongdao Wu ◽  
Xinbing Yu ◽  
Changling Ma ◽  
...  
Keyword(s):  
Schistosoma Japonicum ◽  
Clonorchis Sinensis ◽  
Wistar Rat ◽  
Cross Reactivity ◽  
Releasing Factors

Cross-Reactivity between IgE-Binding Proteins from Anisakis Simplex and Dermatophagoides Pteronyssinus

2005 ◽  
Vol 18 (4) ◽  
pp. 671-675 ◽  
Author(s):  
R. Bernardini ◽  
G. Mistrello ◽  
E. Novembre ◽  
D. Roncarolo ◽  
S. Zanotta ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Binding Proteins ◽  
Specific Ige ◽  
Dermatophagoides Pteronyssinus ◽  
Cross Reactivity ◽  
Anisakis Simplex ◽  
Cross Reaction ◽  
Ige Binding ◽  
Sds Page ◽  
The Cross

An association was found between Anisakis simplex (As) and Dermatophagoides pteronyssinus (Dp) sensitization. One recent study shows a cross-reactivity between As and Dp and tropomyosin (tr) is suspected as being one of the proteins responsible of this cross-reaction. The aim of our study was: 1) to confirm the cross-reactivity between Dp and As; 2) to determine the importance of tr in this cross reaction. SDS-PAGE analysis of Dp and As (metabolic and somatic) extracts was carried out. Then an IgE immunoblotting test using serum from a patient who had specific IgE only to Dp and As and immunoblotting inhibition experiments using Dp extract and tr as inhibitors were performed. We found that patient's serum reacted: 1) against larval As antigens with a molecular weight (mw) of 25 kilodalton (kD) and a mw > 100 kD, 2) against various metabolic As antigens with a mw > 100 kD, a mw ranging approximately from 35 to 50 kD, and a mw around 20 kD, and 3) against Dp proteins with mw between 35 and 55 kD. Preincubation of patient's serum with Dp extract caused the disappearance of reactivity against antigens with a mw > 100 kD in both larval and metabolic As extracts and against proteins with mw ranging approximately from 35 to 50 kD in the metabolic As extract. Preincubation of patient's serum with As extract caused the disappearance of reactivity against antigens with mw between 35 and 55 kD in the Dp extract. Pre-incubation of patient's serum with tr did not induce any change in the immunoblotting profile. The results show that 1) cross-reactive components between Dp and As are some proteins with a mw ranging approximately from 35 to 50 kD and with a mw > 100 kD, and 2) tr is not involved in cross-reactivity between As and Dp.

Cross-reactivity of Anisakis simplex: possible role of Ani s 2 and Ani s 3

2006 ◽  
Vol 0 (0) ◽  
pp. 060720080827026-??? ◽  
Author(s):  
Fabrizio Guarneri ◽  
Claudio Guarneri ◽  
Salvatore Benvenga
Keyword(s):  
Cross Reactivity ◽  
Anisakis Simplex

Cross-reactivity induced by Anisakis simplex and Toxocara canis in mice

2003 ◽  
Vol 77 (4) ◽  
pp. 331-334 ◽  
Author(s):  
M.J. Perteguer ◽  
C. Águila ◽  
S. Fenoy ◽  
J.L. Guillén
Keyword(s):  
Toxocara Canis ◽  
Cross Reactivity ◽  
Rodent Model ◽  
Anisakis Simplex ◽  
Cross Reactions

AbstractThe aim of this study was to verify whether cross-reactivity appeared between Toxocara canis and Anisakis simplex in an experimental rodent model. No cross-reactions were detected using sera from mice infected with T. canis eggs. When responses obtained against T. canis ES antigen using sera from BALB/c and C57BL/10 mice infected with T. canis eggs were compared with those obtained by testing sera from mice infected with one A. simplex L3, an increase in cross-reactions was observed using the C57BL/10 strain.

Multiple antibody reactivities to citrullinated antigens in sera from patients with rheumatoid arthritis: association with HLA-DRB1 alleles

2008 ◽  
Vol 68 (5) ◽  
pp. 736-743 ◽  
Author(s):  
O Snir ◽  
M Widhe ◽  
C von Spee ◽  
J Lindberg ◽  
L Padyukov ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Shared Epitope ◽  
Type Ii Collagen ◽  
Cross Reactivity ◽  
Igg Antibodies ◽  
Serum Samples ◽  
Hla Dr ◽  
Citrullinated Proteins ◽  
The Relationship ◽  
Citrullinated Protein

Background:Autoantibodies to cyclic citrullinated peptides (anti-CCP) are present in most patients with rheumatoid arthritis (RA), and associate with HLA-DRB1 shared epitope (SE) alleles.Objective:To investigate reactivities of anti-CCP to various citrullinated proteins/peptides, which represent potential autoantigens in RA, and to examine the relationship between such antibodies, and their association with genetic variants within HLA-DRB1 SE alleles.Methods:Serum samples from 291 patients with established RA and 100 sex- and age-matched healthy subjects were included in this study. Sera were first analysed for presence of anti-CCP antibodies and further for IgG and IgA antibodies towards candidate autoantigens in both their native and citrullinated form including: fibrinogen, α-enolase peptide-1 and the C1-epitope of type II collagen (C1III). Antibody specificity was confirmed by cross-reactivity tests. HLA-DR genotyping was performed.Results:72% of patients with RA were anti-CCP positive. Among the candidate autoantigens examined, IgG antibodies to citrullinated fibrinogen were found in 66% of patients’ sera and in 41% for both citrullinated α-enolase peptide-1 and citrullinated C1III. These antibodies were mainly seen in the anti-CCP-positive patient group; they were specific for their respective antigen and displayed limited cross reactivity. IgA responses were also detected, but less frequently than IgG. Anti-CCP and anti-citrullinated protein antibodies were associated with HLA-DRB1*04 rather than with HLA-DRB1*01 alleles.Conclusions:Antibodies directed against several citrullinated antigens are present in CCP-positive RA, with many patients displaying multireactivity. All specific reactivities were primarily associated with the HLA-DRB1*04 alleles, suggesting common pathways of anti-citrulline immunity.

Antigenic cross-reactivity in mice between third-stage larvae of Anisakis simplex and other nematodes

1996 ◽  
Vol 82 (4) ◽  
pp. 378-381 ◽  
Author(s):  
R. Iglesias ◽  
J. Leiro ◽  
F. M. Ubeira ◽  
M. T. Santamarina ◽  
I. Navarrete ◽  
...  
Keyword(s):  
Cross Reactivity ◽  
Anisakis Simplex ◽  
Third Stage

O -glycans as a source of cross-reactivity in determinations of human serum antibodies to Anisakis simplex antigens

2000 ◽  
Vol 30 (4) ◽  
pp. 551-559 ◽  
Author(s):  
Lorenzo ◽  
Romarís ◽  
Iglesias ◽  
Audícana ◽  
Alonso ◽  
...  
Keyword(s):  
Human Serum ◽  
Cross Reactivity ◽  
Anisakis Simplex ◽  
Serum Antibodies

Immunological analysis ofOpisthorchisandClonorchisantigens

1990 ◽  
Vol 64 (2) ◽  
pp. 133-138 ◽  
Author(s):  
S. Sirisinha ◽  
D. Sahassananda ◽  
D. Bunnag ◽  
H. J. Rim
Keyword(s):  
Molecular Weight ◽  
Immunosorbent Assay ◽  
Clonorchis Sinensis ◽  
Cross Reactivity ◽  
Opisthorchis Viverrini ◽  
Metabolic Product ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunological Analysis ◽  
Metabolic Products ◽  
Liver Flukes

ABSTRACTImmunoreactive components ofOpisthorchis viverriniandClonorchis sinensiswere analysed by enzyme-linked immunosorbent assay (ELISA), radioimmunoprecipitation and immunoblotting. Somatic extracts from these two liver flukes as well as from other related parasites, together with the metabolic products, were tested for their reactivities with sera from patients with opisthorchiasis and clonorchiasis. A significant cross-reactivity in the ELISA was noted betweenOpisthorchisandClonorchis. Immunoblotting and radioimmunoprecipitation analyses showed that the 89-kD protein which was previously shown to be a predominant metabolic product ofO. viverrinireacted with sera from both groups of patients. However, an antigen with a molecular weight of 16 kD, apparently a predominant somatic component, appeared to be specific forO. viverrini.

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