Immunological cross-reactivity analysis on recombinant histamine-releasing factors from Schistosoma japonicum, Clonorchis sinensis, and Wistar rat

2006 ◽  
Vol 100 (4) ◽  
pp. 749-754 ◽  
Author(s):  
Xiaoxiang Chen ◽  
Xuchu Hu ◽  
Zhongdao Wu ◽  
Xinbing Yu ◽  
Changling Ma ◽  
...  
Keyword(s):  
Schistosoma Japonicum ◽  
Clonorchis Sinensis ◽  
Wistar Rat ◽  
Cross Reactivity ◽  
Releasing Factors

The Complement Fixation Reaction in Schistosoma japonicum with Cercarial Antigen prepared from Oncomelania hupensis

1935 ◽  
Vol 13 (1) ◽  
pp. 25-40
Author(s):  
Mary N. Andrews
Keyword(s):  
Schistosoma Japonicum ◽  
Positive Reaction ◽  
Complement Fixation ◽  
Clonorchis Sinensis ◽  
Alcoholic Extract ◽  
Human Beings ◽  
Oncomelania Hupensis ◽  
Weak Reaction ◽  
Negative Results ◽  
Fixation Reaction

1. The preparation of a cercarial antigen from infected livers of Oncomelania hupensis the intermediate host of Schistosoma japonicum in China is described.2. The sera of 49 human beings and five dogs have been investigated for the Schistosome complement fixation reaction, using as antigens Bilharzial cercarial antigen, and an alcoholic extract of the livers of Oncomelania hupensis infected with cercariae of S. japonicum.3. Thirty-seven of the 49 sera examined were from cases of Schistosomiasis, and of these 24 gave a positive complement fixation reaction, and 2 others a weak reaction.4. Of the five dogs examined three were infected with Schistosoma japonicum. These gave positive reactions.5. In 24 cases both antigens were used, and the results obtained were almost identical.6. The Bilharzial antigen was used in 33 cases including 25 cases of Schistosomiasis, of which 19 gave a positive reaction.7. In 40 cases the Oncomelania antigen was used. This series included 32 cases of Schistosomiasis of which 23 gave a positive reaction and 3 a weak positive reaction.8. Negative results were obtained with 50 strongly positive Wasserman sera, and with 2 cases of infection with Fasciolopsis buski, and two cases of Clonorchis sinensis. Ascaris and hookworm infestations also gave negative results.

Immunological analysis ofOpisthorchisandClonorchisantigens

1990 ◽  
Vol 64 (2) ◽  
pp. 133-138 ◽  
Author(s):  
S. Sirisinha ◽  
D. Sahassananda ◽  
D. Bunnag ◽  
H. J. Rim
Keyword(s):  
Molecular Weight ◽  
Immunosorbent Assay ◽  
Clonorchis Sinensis ◽  
Cross Reactivity ◽  
Opisthorchis Viverrini ◽  
Metabolic Product ◽  
Enzyme Linked Immunosorbent Assay ◽  
Immunological Analysis ◽  
Metabolic Products ◽  
Liver Flukes

ABSTRACTImmunoreactive components ofOpisthorchis viverriniandClonorchis sinensiswere analysed by enzyme-linked immunosorbent assay (ELISA), radioimmunoprecipitation and immunoblotting. Somatic extracts from these two liver flukes as well as from other related parasites, together with the metabolic products, were tested for their reactivities with sera from patients with opisthorchiasis and clonorchiasis. A significant cross-reactivity in the ELISA was noted betweenOpisthorchisandClonorchis. Immunoblotting and radioimmunoprecipitation analyses showed that the 89-kD protein which was previously shown to be a predominant metabolic product ofO. viverrinireacted with sera from both groups of patients. However, an antigen with a molecular weight of 16 kD, apparently a predominant somatic component, appeared to be specific forO. viverrini.

Evaluation of the mutagenicity and the tumor-promoting activity of parasite extracts: Schistosoma japonicum and Clonorchis sinensis

1989 ◽  
Vol 224 (2) ◽  
pp. 229-233 ◽  
Author(s):  
Akira Ishii ◽  
Hiroyuki Matsuoka ◽  
Toshiki Aji ◽  
Hikoya Hayatsu ◽  
Yusuke Wataya ◽  
...  
Keyword(s):  
Schistosoma Japonicum ◽  
Clonorchis Sinensis

Reactivity of anti-tegument monoclonal antibodies with target epitopes in different worm tissues and developmental stages ofSchistosoma mansoni

Parasitology ◽  
1989 ◽  
Vol 98 (2) ◽  
pp. 213-225 ◽  
Author(s):  
Suda Riengrojpitak ◽  
Maria Vojvodic ◽  
C. Boot ◽  
R. A. Wilson
Keyword(s):  
Protein Synthesis ◽  
Monoclonal Antibodies ◽  
Schistosoma Japonicum ◽  
Indirect Immunofluorescence ◽  
Developmental Stages ◽  
Cross Reactivity ◽  
Tissue Specific ◽  
Different Tissues

SUMMARYSixteen monoclonal antibodies (MABs) were selected for their reactivity with adult schistosome tegument. The distribution of target epitopes in different tissues of the adult and in various developmental stages was investigated by indirect immunofluorescence. The distinct patterns of reactivity of the MABs permitted their classification into 9 groups. The distribution of epitopes in larvae, particularly 3 h schistosomula, generally mirrored that in adults. A change in distribution of epitopes coincided with transformation from cercaria to schistosomulum providing a marker for this process. Two MABs reacted with surface membranes of intact cercariae, and 5 with the surfaces of intact 3 h schistosomula. The target epitopes of these 5 MABs were present in the tegument of adults, but not accessible externally. These observations suggest masking of antigens, not shedding, in the course of development. Indeed, no MAB reacted with intact lung or liver worms unless they were damaged. Three MABs reacted with membranes of the tegument and most other tissues, implying properties in common. Evidence for shared functions between epithelia was provided by the reactivity of 2 MABs with the tegument and protonephridia. Eight MABs bound both to the tegument and its cell bodies. Since the latter contains the machinery for protein synthesis, it is difficult to explain why 5 MABs bound to the tegument alone. Little cross-reactivity was found with the tissues of the tapewormHymenolepis, rather more with the closely relatedSchistosoma japonicum. It was concluded that tegument antigens are to a degree tissue specific but definitely not stage specific.

scholarly journals Serodiagnostic antigens of Clonorchis sinensis identified and evaluated by high-throughput proteogenomics

2020 ◽  
Vol 14 (12) ◽  
pp. e0008998
Author(s):  
Pyo Yun Cho ◽  
Ji-Yun Lee ◽  
Tae Im Kim ◽  
Jin-Ho Song ◽  
Sung-Jong Hong ◽  
...  
Keyword(s):  
Large Scale ◽  
Point Of Care ◽  
Clonorchis Sinensis ◽  
Cross Reactivity ◽  
In Vitro Translation ◽  
Translation System ◽  
Antigenic Proteins ◽  
Human Sera ◽  
Expressed Sequence

Clonorchiasis caused by Clonorchis sinensis is endemic in East Asia; approximately 15 million people have been infected thus far. To diagnose the infection, serodiagnostic tests with excellent functionality should be performed. First, 607 expressed sequence tags encoding polypeptides with a secretory signal were expressed into recombinant proteins using an in vitro translation system. By protein array-based screening using C. sinensis-infected sera, 18 antigen candidate proteins were selected and assayed for cross-reactivity against Opisthorchis viverrini-infected sera. Of the six antigenic proteins selected, four were synthesized on large scale in vitro and evaluated for antigenicity against the flukes-infected human sera using ELISA. CsAg17 antigen showed the highest sensitivity (77.1%) and specificity (71.2%). The sensitivity and specificity of the bacterially produced CsAg17-28GST fusion antigen was similar to those of CsAg17 antigen. CsAg17 antigen can be used to develop point-of-care serodiagnostic tests for clonorchiasis.

Ultrastructure of Cercarial Tails

1974 ◽  
Vol 32 ◽  
pp. 180-181
Author(s):  
Richard S. Demaree ◽  
Donald M. Wootton
Keyword(s):  
Schistosoma Japonicum ◽  
Intermediate Hosts ◽  
Suitable Host ◽  
Ultrastructural Studies ◽  
Tail Structure

Cercariae (juvenile trematodes with tails) emerge from mollusk intermediate hosts and swim toward definitive hosts or encystment objects. The locomotor power is furnished by the tail. Upon reaching a suitable host or encystment object, the tail is cast off and the cercariae penetrate and/or encyst. Ultrastructural studies of cercariae are sparse. There is even lessUltrastructural studies of cercariae are sparse. There is even less information about the tail structure; and body-to-tail morphology has been documented only for Acanthatrium oregonense and Schistosoma japonicum.

An Electron Microscopic Study of the Acute Effects of Hypothalamic Releasing Factors on the Anterior Pituitary Gland

1968 ◽  
Vol 26 ◽  
pp. 232-233
Author(s):  
P.W. Coates ◽  
E.A. Ashby ◽  
L. Krulich ◽  
A. Dhariwal ◽  
S. McCann
Keyword(s):  
Anterior Pituitary ◽  
Microscopic Investigation ◽  
Uranyl Acetate ◽  
Electron Microscopic Investigation ◽  
Electron Microscopic ◽  
Tissue Samples ◽  
Thin Sections ◽  
Dense Membrane ◽  
Membrane Bound ◽  
Releasing Factors

The morphologic effects on somatotrophs of crude sheep hypothalamic extract prepared from stalk-median eminence were studied by electron microscopy in conjunction with concurrently run bioassays performed on the same tissue samples taken from young adult male Sherman rats.Groups were divided into uninjected controls and injected experimentals sacrificed at 5', 15', and 30' after injection. Half of each anterior pituitary was prepared for electron microscopic investigation, the other half for bioassay. Fixation using collidine buffered osmium tetroxide was followed by dehydration and embedment in Maraglas. Uranyl acetate and lead citrate were used as stains. Thin sections were examined in a Philips EM 200.Somatotrophs from uninjected controls appeared as described in the literature (Fig. 1). In addition to other components, these cells contained moderate numbers of spherical, electron-dense, membrane-bound granules approximately 350 millicrons in diameter.

Surface epithelial ultrastructure of the polycystic ovary in the BB wistar rat

1986 ◽  
Vol 44 ◽  
pp. 252-253
Author(s):  
R. C. Kaufmann ◽  
F. K. Khosho ◽  
K. S. Amankwah
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Wistar Rats ◽  
Polycystic Ovary ◽  
Wistar Rat ◽  
Polycystic Ovaries ◽  
Chemical Diabetes ◽  
Scanning Electron

Diabetes decreases the fertility of females, but the mechanisms are not completely understood. In our investigations, we have found that 13% of the female BB Wistar rats that spontaneously developed chemical diabetes had persistent estrous. In this study the ovaries of these rats were examined by scanning electron microscopy(SEM) and compared to normal-cycling controls as well as to rats that had developed polycystic ovaries(PCO) by exposure to constant 1ight.

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